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The map label for this gene is ygfA [H]

Identifier: 85373865

GI number: 85373865

Start: 1075119

End: 1075661

Strand: Reverse

Name: ygfA [H]

Synonym: ELI_05190

Alternate gene names: 85373865

Gene position: 1075661-1075119 (Counterclockwise)

Preceding gene: 85373866

Following gene: 85373864

Centisome position: 35.24

GC content: 62.8

Gene sequence:

>543_bases
TTGCGCAAGGCCCGCCGCGAACATGTCGCGACCCAACCGCCATCGATACGTGCGCTGCTGTTCAATCGACCGCCGGCCCC
GCTTCTCGACATGATCGACGAGGATGCGGTCATTGGTCTCTACCACGCTACGGCAGACGAAGCGCCGACGACATCCTATA
CCAAGTTCTTCTACGAACGCGGCCATCGCATCGCCCTGCCCCGTTTCAGCAGCGCCAATGAGCCCATGACGTTTGCAGAG
CACACGGATCCCTTCGACGAAAGCGATCTTGTCGCAGGGCCCTTCGAGCTGCGCCAACCCGTGGAGGATGCGCCGCGGCT
GGAGCCGGACTGCCTGCTCATCCCGTTGATCGGTTTCACGGCAACCGGAGAGCGCCTCGGCCAGGGCGGTGGGCATTACG
ACCAGTGGCTTGCCGATCATCAAGGCCGGACCAAGATCGGTCTTGCCTGGGATGTACAGCTGGTCGAGAGCCTGCCCATC
GAACCGCACGATCAGCGGCTCGATGCTGTCGTCACTCCGACGCGCCTGTACGGACCATTCTGA

Upstream 100 bases:

>100_bases
TCGCATCGCTGCGGGCGGACAGTCGCCCTTTGGGCCCTTCAGGCCGATGATAACGTGAGGATTGATAGCCTTGGAGATTA
AGGCTCAACTACGTCGAGAA

Downstream 100 bases:

>100_bases
TGCGAGACCAACCCACTTGGCGAATCCCGATCGGGCTCCTTGCACTCACGCTAGGCCTGACGGTCTATGCCGTGCTCATT
GCACGCTACGTGCCGGAGTG

Product: 5-formyltetrahydrofolate cyclo-ligase

Products: ADP; phosphate; 5,10-methenyltetrahydrofolate

Alternate protein names: NA

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MRKARREHVATQPPSIRALLFNRPPAPLLDMIDEDAVIGLYHATADEAPTTSYTKFFYERGHRIALPRFSSANEPMTFAE
HTDPFDESDLVAGPFELRQPVEDAPRLEPDCLLIPLIGFTATGERLGQGGGHYDQWLADHQGRTKIGLAWDVQLVESLPI
EPHDQRLDAVVTPTRLYGPF

Sequences:

>Translated_180_residues
MRKARREHVATQPPSIRALLFNRPPAPLLDMIDEDAVIGLYHATADEAPTTSYTKFFYERGHRIALPRFSSANEPMTFAE
HTDPFDESDLVAGPFELRQPVEDAPRLEPDCLLIPLIGFTATGERLGQGGGHYDQWLADHQGRTKIGLAWDVQLVESLPI
EPHDQRLDAVVTPTRLYGPF
>Mature_180_residues
MRKARREHVATQPPSIRALLFNRPPAPLLDMIDEDAVIGLYHATADEAPTTSYTKFFYERGHRIALPRFSSANEPMTFAE
HTDPFDESDLVAGPFELRQPVEDAPRLEPDCLLIPLIGFTATGERLGQGGGHYDQWLADHQGRTKIGLAWDVQLVESLPI
EPHDQRLDAVVTPTRLYGPF

Specific function: Unknown

COG id: COG0212

COG function: function code H; 5-formyltetrahydrofolate cyclo-ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5-formyltetrahydrofolate cyclo-ligase family [H]

Homologues:

Organism=Escherichia coli, GI1789278, Length=149, Percent_Identity=32.8859060402685, Blast_Score=70, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002698 [H]

Pfam domain/function: PF01812 5-FTHF_cyc-lig [H]

EC number: 6.3.3.2

Molecular weight: Translated: 20199; Mature: 20199

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKARREHVATQPPSIRALLFNRPPAPLLDMIDEDAVIGLYHATADEAPTTSYTKFFYER
CCCCHHHHCCCCCCCEEEEEECCCCCHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHC
GHRIALPRFSSANEPMTFAEHTDPFDESDLVAGPFELRQPVEDAPRLEPDCLLIPLIGFT
CCEEECCCCCCCCCCCEECCCCCCCCCCCEEECCHHHHCCHHHCCCCCCCEEEEECCCCC
ATGERLGQGGGHYDQWLADHQGRTKIGLAWDVQLVESLPIEPHDQRLDAVVTPTRLYGPF
CCCHHHCCCCCCHHHHHHCCCCCEEEEEEEHHHHHHHCCCCCCCCCCCEEECCHHHCCCC
>Mature Secondary Structure
MRKARREHVATQPPSIRALLFNRPPAPLLDMIDEDAVIGLYHATADEAPTTSYTKFFYER
CCCCHHHHCCCCCCCEEEEEECCCCCHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHC
GHRIALPRFSSANEPMTFAEHTDPFDESDLVAGPFELRQPVEDAPRLEPDCLLIPLIGFT
CCEEECCCCCCCCCCCEECCCCCCCCCCCEEECCHHHHCCHHHCCCCCCCEEEEECCCCC
ATGERLGQGGGHYDQWLADHQGRTKIGLAWDVQLVESLPIEPHDQRLDAVVTPTRLYGPF
CCCHHHCCCCCCHHHHHHCCCCCEEEEEEEHHHHHHHCCCCCCCCCCCEEECCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 5-formyltetrahydrofolate

Specific reaction: ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]