Click here to switch to the map view.

The map label for this gene is folE [H]

Identifier: 85373831

GI number: 85373831

Start: 1031112

End: 1031723

Strand: Direct

Name: folE [H]

Synonym: ELI_05020

Alternate gene names: 85373831

Gene position: 1031112-1031723 (Clockwise)

Preceding gene: 85373830

Following gene: 85373833

Centisome position: 33.78

GC content: 60.29

Gene sequence:

>612_bases
ATGGCCTGCGAAGCCGACTATGTCGAAGATCCCGCGATCAAGAAGCCGGACGTGCCCGAGCACGTGCAAGATGCCATCCG
CACATTGATCGAATGGTCGGGCGACGATCCGGCACGCGAAGGTCTGCTGGACACTCCCAAGCGGGTCGGGCGTGCCTGGC
TCGAATATTGCCAGGGTTACAAAGAGGATCCTGCGATCCACCTCAGCCGGGTATTCGAGGAGGTTGGGGGCTATGACGAG
ATCGTCTTGCTCAAAGACATCCCCTTCCAGAGCCATTGCGAGCATCACATGGCGCCGATCACCGGAAAGGCGGCGATTGC
CTACCTTCCCAGAGACAAGGTCGTCGGTATCTCCAAGCTCGCCCGCGTACTCCACGGCTTTGCCCGCCGGCTGCAAATCC
AGGAACGTCTGACGGCCGAAGTCGCGAACTGCATCTGGGACAATCTCGAGCCAGAGGGCGTTGCAGTCGTGATCGAAGCC
CAGCATGGCTGCATGACCGGTCGCGGTGTACGAACGCCCGGGGTCAGCATGGTCACATCAAAGCTGTGCGGCTGTTTCCT
CGATGACGAACGGAGCCGCAAGGAAGTCCTCAGCCTGATGGGTTACGGTTGA

Upstream 100 bases:

>100_bases
GGACCAAAGTTGGGCTTTACCGCTCAGGCACTTTCCGCTTTGGCGCTCGCACATATCGGCATGGCCCTTGGTGCCGTGCC
CAGAAAGAGAGGTAGACGCC

Downstream 100 bases:

>100_bases
GCCTTGGTCAGGCAGTAATGCCGCGCTTGAGGATCTGCATCGCCCGCTCGGGAACGTCTTCGATGGCATCGTCATCCTTC
GCCCAGACAGCGTAGCGAAG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MACEADYVEDPAIKKPDVPEHVQDAIRTLIEWSGDDPAREGLLDTPKRVGRAWLEYCQGYKEDPAIHLSRVFEEVGGYDE
IVLLKDIPFQSHCEHHMAPITGKAAIAYLPRDKVVGISKLARVLHGFARRLQIQERLTAEVANCIWDNLEPEGVAVVIEA
QHGCMTGRGVRTPGVSMVTSKLCGCFLDDERSRKEVLSLMGYG

Sequences:

>Translated_203_residues
MACEADYVEDPAIKKPDVPEHVQDAIRTLIEWSGDDPAREGLLDTPKRVGRAWLEYCQGYKEDPAIHLSRVFEEVGGYDE
IVLLKDIPFQSHCEHHMAPITGKAAIAYLPRDKVVGISKLARVLHGFARRLQIQERLTAEVANCIWDNLEPEGVAVVIEA
QHGCMTGRGVRTPGVSMVTSKLCGCFLDDERSRKEVLSLMGYG
>Mature_202_residues
ACEADYVEDPAIKKPDVPEHVQDAIRTLIEWSGDDPAREGLLDTPKRVGRAWLEYCQGYKEDPAIHLSRVFEEVGGYDEI
VLLKDIPFQSHCEHHMAPITGKAAIAYLPRDKVVGISKLARVLHGFARRLQIQERLTAEVANCIWDNLEPEGVAVVIEAQ
HGCMTGRGVRTPGVSMVTSKLCGCFLDDERSRKEVLSLMGYG

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=177, Percent_Identity=43.5028248587571, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI4503949, Length=177, Percent_Identity=43.5028248587571, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI66932970, Length=147, Percent_Identity=42.1768707482993, Blast_Score=120, Evalue=6e-28,
Organism=Homo sapiens, GI66932972, Length=137, Percent_Identity=44.5255474452555, Blast_Score=118, Evalue=4e-27,
Organism=Escherichia coli, GI1788476, Length=172, Percent_Identity=36.6279069767442, Blast_Score=114, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17560486, Length=203, Percent_Identity=42.8571428571429, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6321706, Length=184, Percent_Identity=45.6521739130435, Blast_Score=164, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24656782, Length=178, Percent_Identity=43.8202247191011, Blast_Score=144, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24656796, Length=178, Percent_Identity=43.8202247191011, Blast_Score=144, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24656791, Length=178, Percent_Identity=43.8202247191011, Blast_Score=143, Evalue=6e-35,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 22600; Mature: 22469

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACEADYVEDPAIKKPDVPEHVQDAIRTLIEWSGDDPAREGLLDTPKRVGRAWLEYCQGY
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHCCC
KEDPAIHLSRVFEEVGGYDEIVLLKDIPFQSHCEHHMAPITGKAAIAYLPRDKVVGISKL
CCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCCCCCEEEECCCCCCCCHHHH
ARVLHGFARRLQIQERLTAEVANCIWDNLEPEGVAVVIEAQHGCMTGRGVRTPGVSMVTS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHH
KLCGCFLDDERSRKEVLSLMGYG
HHHHHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
ACEADYVEDPAIKKPDVPEHVQDAIRTLIEWSGDDPAREGLLDTPKRVGRAWLEYCQGY
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHCCC
KEDPAIHLSRVFEEVGGYDEIVLLKDIPFQSHCEHHMAPITGKAAIAYLPRDKVVGISKL
CCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCCCCCEEEECCCCCCCCHHHH
ARVLHGFARRLQIQERLTAEVANCIWDNLEPEGVAVVIEAQHGCMTGRGVRTPGVSMVTS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHH
KLCGCFLDDERSRKEVLSLMGYG
HHHHHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]