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The map label for this gene is bioB

Identifier: 85373811

GI number: 85373811

Start: 1015230

End: 1016255

Strand: Direct

Name: bioB

Synonym: ELI_04920

Alternate gene names: 85373811

Gene position: 1015230-1016255 (Clockwise)

Preceding gene: 85373810

Following gene: 85373813

Centisome position: 33.26

GC content: 64.42

Gene sequence:

>1026_bases
ATGACCCCCATCCGTACCAATTGGAGCCGTGACGAAATCGCGGCCCTGTTCGAACAACCGTTCACCGAGCTGCTATTCCA
GGCCGCCACGGTCCACCGCGCCTACCATCCGCCCGAGCAGGTGCAGCTCTGCACACTGCTGTCGATCAAGACCGGCGGGT
GCCCGGAGGATTGCGGCTATTGTTCGCAGTCGGTGAAGGCCGATAGCGGCGTCGAAGCGACCAAGCTGATGGACGTCCAG
CGCGTGCTGCAATCGGCGGCGCAGGCCAAGGATGCGGGCTCGCAGCGCTTCTGCATGGGCGCGGCGTGGCGCAATCCCAA
GGACCGCGACATGCCCGCGATCGTCGAGATCGTGAAGGGCGTGCGCGACATGGGGCTGGAGACCTGCATGACGCTGGGCA
TGCTGACGCCGAAGCAGGCGGACATGCTCAAGGACGCGGGCCTCGATTACTACAACCACAACGTCGACACCGGGCCGGAA
TATTACGAGCGCGTAATCTCGACCCGCAATTACCAGGACCGTCTCGACACGCTCCAGAACGTCCGCGATGCCGGGATCAA
CGTGTGCAGCGGCGGGATCGTCGGCATGGGCGAAACGCGCGAGGACCGCGTGGGCTTCGTCCACACGCTCGCCACGCTCG
AACGCCATCCAGAAAGCGTGCCGGTCAATGCGTTGGTGCCGGTCAAGGGCACGGTGCTCGGCGACATGCTGGCCGACACG
CCGCTCGCCAAGATCGACGATATCGAATTCGTCCGCACCGTGGCAGTGGCGCGCATCACCATGCCGCTGAGCATGGTGCG
GCTGTCCGCAGGACGCGAGAGCATGAGCGAAGCGACGCAGGCGCTGTGTTTCATGGCGGGCGCGAACTCGATCTTCACCG
GCGACAAGCTGCTGACGGCAGCCAATGCCGGCGATGACAAGGACGCGGCGCTGTTCGACAAGCTCGGGTTAACAGCCTTG
CAAGGTGAAGAACCCTTGCGACGCGCCAAAGATGAAGCAGGGAAGGCGGCGATTCCCGCGGAGTGA

Upstream 100 bases:

>100_bases
CTTGCATCAAGCTCGCTGAAGGCATGGACTTTTGCTATCAGTTCTCTGCTGAGGGCTACAAACTATATTTGATCTTCAAG
GACGTCACGAGACAAACTCA

Downstream 100 bases:

>100_bases
CTCTCGGGTTCTCAGTACGCGAGCTCCTCCCGCACGCCCGCAATAGCGAGAAACTGAAGATCGGATTGTGTGCGCTGGGG
GAGGACGAATGGCTCCAACC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MTPIRTNWSRDEIAALFEQPFTELLFQAATVHRAYHPPEQVQLCTLLSIKTGGCPEDCGYCSQSVKADSGVEATKLMDVQ
RVLQSAAQAKDAGSQRFCMGAAWRNPKDRDMPAIVEIVKGVRDMGLETCMTLGMLTPKQADMLKDAGLDYYNHNVDTGPE
YYERVISTRNYQDRLDTLQNVRDAGINVCSGGIVGMGETREDRVGFVHTLATLERHPESVPVNALVPVKGTVLGDMLADT
PLAKIDDIEFVRTVAVARITMPLSMVRLSAGRESMSEATQALCFMAGANSIFTGDKLLTAANAGDDKDAALFDKLGLTAL
QGEEPLRRAKDEAGKAAIPAE

Sequences:

>Translated_341_residues
MTPIRTNWSRDEIAALFEQPFTELLFQAATVHRAYHPPEQVQLCTLLSIKTGGCPEDCGYCSQSVKADSGVEATKLMDVQ
RVLQSAAQAKDAGSQRFCMGAAWRNPKDRDMPAIVEIVKGVRDMGLETCMTLGMLTPKQADMLKDAGLDYYNHNVDTGPE
YYERVISTRNYQDRLDTLQNVRDAGINVCSGGIVGMGETREDRVGFVHTLATLERHPESVPVNALVPVKGTVLGDMLADT
PLAKIDDIEFVRTVAVARITMPLSMVRLSAGRESMSEATQALCFMAGANSIFTGDKLLTAANAGDDKDAALFDKLGLTAL
QGEEPLRRAKDEAGKAAIPAE
>Mature_340_residues
TPIRTNWSRDEIAALFEQPFTELLFQAATVHRAYHPPEQVQLCTLLSIKTGGCPEDCGYCSQSVKADSGVEATKLMDVQR
VLQSAAQAKDAGSQRFCMGAAWRNPKDRDMPAIVEIVKGVRDMGLETCMTLGMLTPKQADMLKDAGLDYYNHNVDTGPEY
YERVISTRNYQDRLDTLQNVRDAGINVCSGGIVGMGETREDRVGFVHTLATLERHPESVPVNALVPVKGTVLGDMLADTP
LAKIDDIEFVRTVAVARITMPLSMVRLSAGRESMSEATQALCFMAGANSIFTGDKLLTAANAGDDKDAALFDKLGLTALQ
GEEPLRRAKDEAGKAAIPAE

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=317, Percent_Identity=60.2523659305994, Blast_Score=399, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6321725, Length=318, Percent_Identity=49.685534591195, Blast_Score=350, Evalue=2e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_ERYLH (Q2NB65)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_457873.1
- STRING:   Q2NB65
- GeneID:   3870147
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_04920
- NMPDR:   fig|314225.3.peg.2087
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- PhylomeDB:   Q2NB65
- ProtClustDB:   CLSK2391555
- BioCyc:   ELIT314225:ELI_04920-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 37046; Mature: 36914

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPIRTNWSRDEIAALFEQPFTELLFQAATVHRAYHPPEQVQLCTLLSIKTGGCPEDCGY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEECCCCCCHHHHH
CSQSVKADSGVEATKLMDVQRVLQSAAQAKDAGSQRFCMGAAWRNPKDRDMPAIVEIVKG
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHH
VRDMGLETCMTLGMLTPKQADMLKDAGLDYYNHNVDTGPEYYERVISTRNYQDRLDTLQN
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
VRDAGINVCSGGIVGMGETREDRVGFVHTLATLERHPESVPVNALVPVKGTVLGDMLADT
HHHHCCEEECCCEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHCC
PLAKIDDIEFVRTVAVARITMPLSMVRLSAGRESMSEATQALCFMAGANSIFTGDKLLTA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEECCCHHEEE
ANAGDDKDAALFDKLGLTALQGEEPLRRAKDEAGKAAIPAE
CCCCCCHHHHHHHHHCCHHHCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TPIRTNWSRDEIAALFEQPFTELLFQAATVHRAYHPPEQVQLCTLLSIKTGGCPEDCGY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEECCCCCCHHHHH
CSQSVKADSGVEATKLMDVQRVLQSAAQAKDAGSQRFCMGAAWRNPKDRDMPAIVEIVKG
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHH
VRDMGLETCMTLGMLTPKQADMLKDAGLDYYNHNVDTGPEYYERVISTRNYQDRLDTLQN
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
VRDAGINVCSGGIVGMGETREDRVGFVHTLATLERHPESVPVNALVPVKGTVLGDMLADT
HHHHCCEEECCCEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHCC
PLAKIDDIEFVRTVAVARITMPLSMVRLSAGRESMSEATQALCFMAGANSIFTGDKLLTA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEECCCHHEEE
ANAGDDKDAALFDKLGLTALQGEEPLRRAKDEAGKAAIPAE
CCCCCCHHHHHHHHHCCHHHCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA