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The map label for this gene is dfrA [H]

Identifier: 85373804

GI number: 85373804

Start: 1007351

End: 1008388

Strand: Direct

Name: dfrA [H]

Synonym: ELI_04885

Alternate gene names: 85373804

Gene position: 1007351-1008388 (Clockwise)

Preceding gene: 85373803

Following gene: 85373805

Centisome position: 33.0

GC content: 64.07

Gene sequence:

>1038_bases
ATGGCTGGCGCTGTTCTGGTAACCGGCGGCACCGGCTATATCGGCGGTGAGCTGATCAAGCAGTTGCTGGCCAAGGGATG
GACGGTGCACACCACCATCCGCGACACGGCCAAGAGCGAGGTGCGGCTGTTCGACCGCTTCGGCCAGCCGCCTGCGGACA
AGCTCAAGGTCTTCCAGGCCGAATTGCTGAGCGACGATGGCTGGGCCGAAGCAGTTGCCGGATGCACCCACGTCGCCCAC
GTCGCTTCGCCGGTTTCCGACACCACGCCGGACGACGAGGACGAGCTGATCGTCCCGGCTCGCGAAGGCACTCTGCGCGC
GCGCCGCTTTGCCCGGCAAGCAGGCGTGCAGCGCTTCGTCCATACCAGCTCGATCGCCGCCGTCGCCTATGGTCGCGGGG
GCAAGGAATACACCTCCACCGAGGCGGACTGGACCGACGTCAATTCGCCGGACACCACCGCCTATGCCAAGTCCAAGACC
ATTGCCGAACGCACAGCGCGGGACTGGATCGCGGCGGAGGGCGCCAAAGACGGAAAGAGCACGGAATATGTCTCGATCAA
TCCCGTGCTGGTGCTCGGCCCGGTCGACAGCAACGACTTCTCCGCCTCGGTCACTCCGGTGACGATGCTGCTGACCGGCA
AGGTACCGATGGCACCTGACATTGGCTTCGGCGTGGTCGATCTGCGCGACGTGGCCGACCTGCATGTGCGCTGTCTCGAA
GCGCCGGGCATCAATGGCGAGCGCTTCATCGCTTCCGGTAAGTTCATGAAGATGATCGACCTCGCGAATGTCATGCGCAG
AGGGCTGACGAAGGAGCAGGCGAAGAAACTGCCCAAGCGGTCAATGCCCGGTTGGGTCGCGAGCCTGTTCATGCTCATCA
ATCCGCCGTTCCGCTCCGTGAAGAACGAGCTGGGCAGGGTGCGCCATTCGGATGCGAGCCATGCCGAGGAAGTGCTGGGC
TGGAAGACGCGGCCCGAGGAAGACAGCATCCTCGATTGCGCGCGCAGCCTGTTCGAACATGGGGTGGTGAAGGTCTAG

Upstream 100 bases:

>100_bases
TGCCGAAGAAGCAAGCTACACCATCCTTCTCCAACTGGGAGACGGATGCGCAGGCTTCGCACGCCGTGCGGTAGCCGGAG
TGGGATGAGGGAGAGAAGGC

Downstream 100 bases:

>100_bases
GTCTGCAACGATCACAATCGGTTGACGCTGCGGCAATGCGCTGGCTAGGCGCAATGCAAACGCACACAGGGGAACTGCAT
GTCCGCCAATATCGCCGAAA

Product: putative dihydroflavonol-4-reductase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MAGAVLVTGGTGYIGGELIKQLLAKGWTVHTTIRDTAKSEVRLFDRFGQPPADKLKVFQAELLSDDGWAEAVAGCTHVAH
VASPVSDTTPDDEDELIVPAREGTLRARRFARQAGVQRFVHTSSIAAVAYGRGGKEYTSTEADWTDVNSPDTTAYAKSKT
IAERTARDWIAAEGAKDGKSTEYVSINPVLVLGPVDSNDFSASVTPVTMLLTGKVPMAPDIGFGVVDLRDVADLHVRCLE
APGINGERFIASGKFMKMIDLANVMRRGLTKEQAKKLPKRSMPGWVASLFMLINPPFRSVKNELGRVRHSDASHAEEVLG
WKTRPEEDSILDCARSLFEHGVVKV

Sequences:

>Translated_345_residues
MAGAVLVTGGTGYIGGELIKQLLAKGWTVHTTIRDTAKSEVRLFDRFGQPPADKLKVFQAELLSDDGWAEAVAGCTHVAH
VASPVSDTTPDDEDELIVPAREGTLRARRFARQAGVQRFVHTSSIAAVAYGRGGKEYTSTEADWTDVNSPDTTAYAKSKT
IAERTARDWIAAEGAKDGKSTEYVSINPVLVLGPVDSNDFSASVTPVTMLLTGKVPMAPDIGFGVVDLRDVADLHVRCLE
APGINGERFIASGKFMKMIDLANVMRRGLTKEQAKKLPKRSMPGWVASLFMLINPPFRSVKNELGRVRHSDASHAEEVLG
WKTRPEEDSILDCARSLFEHGVVKV
>Mature_344_residues
AGAVLVTGGTGYIGGELIKQLLAKGWTVHTTIRDTAKSEVRLFDRFGQPPADKLKVFQAELLSDDGWAEAVAGCTHVAHV
ASPVSDTTPDDEDELIVPAREGTLRARRFARQAGVQRFVHTSSIAAVAYGRGGKEYTSTEADWTDVNSPDTTAYAKSKTI
AERTARDWIAAEGAKDGKSTEYVSINPVLVLGPVDSNDFSASVTPVTMLLTGKVPMAPDIGFGVVDLRDVADLHVRCLEA
PGINGERFIASGKFMKMIDLANVMRRGLTKEQAKKLPKRSMPGWVASLFMLINPPFRSVKNELGRVRHSDASHAEEVLGW
KTRPEEDSILDCARSLFEHGVVKV

Specific function: Unknown

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI71987463, Length=343, Percent_Identity=32.0699708454811, Blast_Score=167, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6324421, Length=360, Percent_Identity=25.8333333333333, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320750, Length=360, Percent_Identity=24.4444444444444, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6321399, Length=359, Percent_Identity=26.1838440111421, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321281, Length=357, Percent_Identity=25.7703081232493, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 37446; Mature: 37315

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGAVLVTGGTGYIGGELIKQLLAKGWTVHTTIRDTAKSEVRLFDRFGQPPADKLKVFQA
CCCCEEEECCCCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ELLSDDGWAEAVAGCTHVAHVASPVSDTTPDDEDELIVPAREGTLRARRFARQAGVQRFV
HHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHH
HTSSIAAVAYGRGGKEYTSTEADWTDVNSPDTTAYAKSKTIAERTARDWIAAEGAKDGKS
HHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TEYVSINPVLVLGPVDSNDFSASVTPVTMLLTGKVPMAPDIGFGVVDLRDVADLHVRCLE
CCEEEECCEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHC
APGINGERFIASGKFMKMIDLANVMRRGLTKEQAKKLPKRSMPGWVASLFMLINPPFRSV
CCCCCCCEEEECCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHCCCHHHH
KNELGRVRHSDASHAEEVLGWKTRPEEDSILDCARSLFEHGVVKV
HHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AGAVLVTGGTGYIGGELIKQLLAKGWTVHTTIRDTAKSEVRLFDRFGQPPADKLKVFQA
CCCEEEECCCCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ELLSDDGWAEAVAGCTHVAHVASPVSDTTPDDEDELIVPAREGTLRARRFARQAGVQRFV
HHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHH
HTSSIAAVAYGRGGKEYTSTEADWTDVNSPDTTAYAKSKTIAERTARDWIAAEGAKDGKS
HHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TEYVSINPVLVLGPVDSNDFSASVTPVTMLLTGKVPMAPDIGFGVVDLRDVADLHVRCLE
CCEEEECCEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHC
APGINGERFIASGKFMKMIDLANVMRRGLTKEQAKKLPKRSMPGWVASLFMLINPPFRSV
CCCCCCCEEEECCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHCCCHHHH
KNELGRVRHSDASHAEEVLGWKTRPEEDSILDCARSLFEHGVVKV
HHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]