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The map label for this gene is smf [H]

Identifier: 85373787

GI number: 85373787

Start: 991583

End: 992686

Strand: Direct

Name: smf [H]

Synonym: ELI_04800

Alternate gene names: 85373787

Gene position: 991583-992686 (Clockwise)

Preceding gene: 85373786

Following gene: 85373788

Centisome position: 32.49

GC content: 68.93

Gene sequence:

>1104_bases
ATGGCAGAGGTCGCTCTCTCGCAAGCCGAAGCTTTCGCCCGCATCCGGCTGCTGCGCTCGCCGCATATCGGGCCGGTGAG
CTATCGCCAGCTAATGGCGCGCTTCGGCACGGCGGAAGCTGCGCTGGAGGCGCTGCCCGATCTCGGCAAGAAAGGCGGCC
GGCAATACAGGCCCGCGCCGCGCGACAGGATCGAGCGCGAAGTCGATGCGGTCCGCGAGGCAGGAGCGCGCTACCTGTTC
CACGACCAGCCCGATTATCCCGCGCTGCTCGCCCAGCTCGACAGCGCGCCGCCTATCCTGACCTTTCGCGGCGACCTGGC
CATTGCCGCACAGACATGCGTCGCCATGGTCGGCGCCCGCAACGCCAGCGCGGCCTCGATCAAGCTGGCACGCGAATTCG
CCAGCGCGCTGGCGGGCGAGGGGATTGCGGTCGTCTCAGGGCTCGCGCGCGGGATCGACGGGGCCTGCCACGAAGGGGCG
TTTCCGGGGACGATCGGGGTCATCGCCAGCGGTATCGACATCGCCTACCCGCCTCAACATGCCGAACTGCAGGAGCGGAT
CGCGAGCGAGGCGCTGCTCCTTGCCGAACAGCCACCGGGCACAGAGCCGCGCGGAAGCCACTTTCCCAGCCGCAACCGCA
TCATTGCCGGGCTGGCGGCGGGCACGCTGGTGGTCGAGGCTGCGCCCAAGTCCGGCTCGCTCATCACCGCGCGGTTGGCG
GGCGAGGCGGGGAGGGAGGTCATGGCGATTCCCGGCTCCCCACTCGATGCGCGCTCGCAAGGGTGCAACCAGCTCATCCG
CGACGGGGCTGTACTGGTGCAGCAGCCGGAAGACGTGGTCGAATTGCTGCAGGGCTTCGACGGCTCGCCGCGCTCGAGTT
TTCGCGAAGCCGCCGCGCTGGAATTCGATCCCGCGCCGGAAGAGCTTGCCGAGGCCGAACCCGCCGACGTCGCGGGTCTG
CTGACAACGGCACCGGTAGCGGTCGACGAATTGATCCGCCAGTCGGGCGAGCCCGCGGCAGCGGTACAACTCGCCTTACT
CGAGCTTGAAATCTCGGGACAATTGACGCGGCACGCTGGCGGGCGTGTATCGCGCACGGCATAG

Upstream 100 bases:

>100_bases
GGTGATCATTGCGGTCATCATCATCTGGCTGCACCGCGCCAATATCCGCCGCCTGCTGCGCGGAGAGGAACCCAGGGTGG
GGAACAAACCGTAGCGCTTG

Downstream 100 bases:

>100_bases
CGAGAGGTCTTTCCGGTCGAGTGGGGGTACGAAAATGAAGAGAGATGCGGATTTCGCAGGGTTTCTGGGCGAGACCTTCG
CCGTCGCCCGCGACGCGCTG

Product: DNA processing chain A

Products: NA

Alternate protein names: DNA-processing chain A [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MAEVALSQAEAFARIRLLRSPHIGPVSYRQLMARFGTAEAALEALPDLGKKGGRQYRPAPRDRIEREVDAVREAGARYLF
HDQPDYPALLAQLDSAPPILTFRGDLAIAAQTCVAMVGARNASAASIKLAREFASALAGEGIAVVSGLARGIDGACHEGA
FPGTIGVIASGIDIAYPPQHAELQERIASEALLLAEQPPGTEPRGSHFPSRNRIIAGLAAGTLVVEAAPKSGSLITARLA
GEAGREVMAIPGSPLDARSQGCNQLIRDGAVLVQQPEDVVELLQGFDGSPRSSFREAAALEFDPAPEELAEAEPADVAGL
LTTAPVAVDELIRQSGEPAAAVQLALLELEISGQLTRHAGGRVSRTA

Sequences:

>Translated_367_residues
MAEVALSQAEAFARIRLLRSPHIGPVSYRQLMARFGTAEAALEALPDLGKKGGRQYRPAPRDRIEREVDAVREAGARYLF
HDQPDYPALLAQLDSAPPILTFRGDLAIAAQTCVAMVGARNASAASIKLAREFASALAGEGIAVVSGLARGIDGACHEGA
FPGTIGVIASGIDIAYPPQHAELQERIASEALLLAEQPPGTEPRGSHFPSRNRIIAGLAAGTLVVEAAPKSGSLITARLA
GEAGREVMAIPGSPLDARSQGCNQLIRDGAVLVQQPEDVVELLQGFDGSPRSSFREAAALEFDPAPEELAEAEPADVAGL
LTTAPVAVDELIRQSGEPAAAVQLALLELEISGQLTRHAGGRVSRTA
>Mature_366_residues
AEVALSQAEAFARIRLLRSPHIGPVSYRQLMARFGTAEAALEALPDLGKKGGRQYRPAPRDRIEREVDAVREAGARYLFH
DQPDYPALLAQLDSAPPILTFRGDLAIAAQTCVAMVGARNASAASIKLAREFASALAGEGIAVVSGLARGIDGACHEGAF
PGTIGVIASGIDIAYPPQHAELQERIASEALLLAEQPPGTEPRGSHFPSRNRIIAGLAAGTLVVEAAPKSGSLITARLAG
EAGREVMAIPGSPLDARSQGCNQLIRDGAVLVQQPEDVVELLQGFDGSPRSSFREAAALEFDPAPEELAEAEPADVAGLL
TTAPVAVDELIRQSGEPAAAVQLALLELEISGQLTRHAGGRVSRTA

Specific function: Essential for efficient chromosomal DNA transformation but not required for plasmid transformation [H]

COG id: COG0758

COG function: function code LU; Predicted Rossmann fold nucleotide-binding protein involved in DNA uptake

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smf family [H]

Homologues:

Organism=Escherichia coli, GI48994932, Length=332, Percent_Identity=34.9397590361446, Blast_Score=158, Evalue=5e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003488 [H]

Pfam domain/function: PF02481 DNA_processg_A [H]

EC number: NA

Molecular weight: Translated: 38339; Mature: 38208

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEVALSQAEAFARIRLLRSPHIGPVSYRQLMARFGTAEAALEALPDLGKKGGRQYRPAP
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCC
RDRIEREVDAVREAGARYLFHDQPDYPALLAQLDSAPPILTFRGDLAIAAQTCVAMVGAR
HHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCC
NASAASIKLAREFASALAGEGIAVVSGLARGIDGACHEGAFPGTIGVIASGIDIAYPPQH
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHCCCCCCCHHHHHHCCCCCCCCCCH
AELQERIASEALLLAEQPPGTEPRGSHFPSRNRIIAGLAAGTLVVEAAPKSGSLITARLA
HHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCEEEEEECCEEEEEECCCCCCEEEEEEC
GEAGREVMAIPGSPLDARSQGCNQLIRDGAVLVQQPEDVVELLQGFDGSPRSSFREAAAL
CCCCCEEEECCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHCC
EFDPAPEELAEAEPADVAGLLTTAPVAVDELIRQSGEPAAAVQLALLELEISGQLTRHAG
CCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEECCCCHHHHCC
GRVSRTA
CCCCCCC
>Mature Secondary Structure 
AEVALSQAEAFARIRLLRSPHIGPVSYRQLMARFGTAEAALEALPDLGKKGGRQYRPAP
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCC
RDRIEREVDAVREAGARYLFHDQPDYPALLAQLDSAPPILTFRGDLAIAAQTCVAMVGAR
HHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCC
NASAASIKLAREFASALAGEGIAVVSGLARGIDGACHEGAFPGTIGVIASGIDIAYPPQH
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHCCCCCCCHHHHHHCCCCCCCCCCH
AELQERIASEALLLAEQPPGTEPRGSHFPSRNRIIAGLAAGTLVVEAAPKSGSLITARLA
HHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCEEEEEECCEEEEEECCCCCCEEEEEEC
GEAGREVMAIPGSPLDARSQGCNQLIRDGAVLVQQPEDVVELLQGFDGSPRSSFREAAAL
CCCCCEEEECCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHCC
EFDPAPEELAEAEPADVAGLLTTAPVAVDELIRQSGEPAAAVQLALLELEISGQLTRHAG
CCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEECCCCHHHHCC
GRVSRTA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7768823; 7542800 [H]