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The map label for this gene is psdht [H]

Identifier: 85373770

GI number: 85373770

Start: 974557

End: 975519

Strand: Reverse

Name: psdht [H]

Synonym: ELI_04715

Alternate gene names: 85373770

Gene position: 975519-974557 (Counterclockwise)

Preceding gene: 85373774

Following gene: 85373769

Centisome position: 31.96

GC content: 67.71

Gene sequence:

>963_bases
ATGCGCTATCACGGCAAAATGATCCGAAAACCCACCCGCGACGGCGTGCTCGAAGCAGCCGCGAAGATCGCAGACATCCT
GCCGCCGACCCCGCTGCTGCCGGTCGAAATCGACGGCACAAATTGCTGGGTAAAGGCGGAGAACCTGCAGCCCGTCGGCG
CGTTCAAGATCCGTGGCGCGTGGCATCGGCTCTCGGCGATGAGCGAGGAGGAGAGGTCGCGCGGCGTGGTCGCGGTGTCC
TCGGGCAATCACGCGCGAGGCGTGGCCTGGGCCGCACGGCGGCTGGGTATCGCCGCGACCATCGTGATGCCGCGCGATGC
GCCGAAGGTGAAGCTGGAAGCGACCAAGGCGCTCGGTGCCGAGGTGGTGCTCTATGAGCGCCCCGGCGAGGATCGCGATG
AAGTGGCCGCGACGCTGATTGCGGAACGCGGCGGCACGCTGGTTCACGCCTTTGGCGATCCGTGGGTCATCGAGGGACAA
GGCAGCGCAGGGATCGAGATTACTGAACAGCTGGGTCGCCAGCCCTCGCGCATCGTCGCTTGTTGCGGCGGCGGAGGCTT
GGCCGCTGGGCTCGCCCTCGCCTGCCCGGACAGCGCGATCGTGCCGGTCGAGCCGGATGGCTGGGACATGGTCGGGCAAG
CATTGAAAGCGGGCGAGATCGTGGCCGTTGGCGACGATGCACCTGCGACCATCTGCGATGCGCTGCAGCCCACGACGACC
AAGCCGGTCAACCTCGCTGTCTTGCAAGGACGCTCGGAGCCCGGCGTCACCGTCAGCGACGATGAAGTCCGCGCGGCGCA
GCGCTTTGCGTTTTCGCACCTGCGCCTCGTCGTGGAACCCGGCGGTGCCGCAGCGCTGGCCGCGGCGATTGCAGGCAAGG
TGTCGCTCGACGCGCACACGGTTCTGATGATCACCGGTGGCAACACCGACGCCGCCAGCTTCGCGGCAACCATTGCCGCC
TGA

Upstream 100 bases:

>100_bases
TTGTGGACGCAGACCGAGCTGACGCCGCCGGCACCGATTACGAGGACTGTGGACATGGAGGGGGCCTTTCGAATGCGAAT
CTTGCGCTGCTCTTAGTCGA

Downstream 100 bases:

>100_bases
TTGACAATCCCCGGCGCCCCCGCCACCAAAGCCGTTCCATAAGGGAAGGGGAACCCATGCAAGCGCAAATGCTCGCACCG
GCCGCCGTGCTGGTGCTGTG

Product: pyridoxal-phosphate dependent enzyme

Products: 2-oxobutanoate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MRYHGKMIRKPTRDGVLEAAAKIADILPPTPLLPVEIDGTNCWVKAENLQPVGAFKIRGAWHRLSAMSEEERSRGVVAVS
SGNHARGVAWAARRLGIAATIVMPRDAPKVKLEATKALGAEVVLYERPGEDRDEVAATLIAERGGTLVHAFGDPWVIEGQ
GSAGIEITEQLGRQPSRIVACCGGGGLAAGLALACPDSAIVPVEPDGWDMVGQALKAGEIVAVGDDAPATICDALQPTTT
KPVNLAVLQGRSEPGVTVSDDEVRAAQRFAFSHLRLVVEPGGAAALAAAIAGKVSLDAHTVLMITGGNTDAASFAATIAA

Sequences:

>Translated_320_residues
MRYHGKMIRKPTRDGVLEAAAKIADILPPTPLLPVEIDGTNCWVKAENLQPVGAFKIRGAWHRLSAMSEEERSRGVVAVS
SGNHARGVAWAARRLGIAATIVMPRDAPKVKLEATKALGAEVVLYERPGEDRDEVAATLIAERGGTLVHAFGDPWVIEGQ
GSAGIEITEQLGRQPSRIVACCGGGGLAAGLALACPDSAIVPVEPDGWDMVGQALKAGEIVAVGDDAPATICDALQPTTT
KPVNLAVLQGRSEPGVTVSDDEVRAAQRFAFSHLRLVVEPGGAAALAAAIAGKVSLDAHTVLMITGGNTDAASFAATIAA
>Mature_320_residues
MRYHGKMIRKPTRDGVLEAAAKIADILPPTPLLPVEIDGTNCWVKAENLQPVGAFKIRGAWHRLSAMSEEERSRGVVAVS
SGNHARGVAWAARRLGIAATIVMPRDAPKVKLEATKALGAEVVLYERPGEDRDEVAATLIAERGGTLVHAFGDPWVIEGQ
GSAGIEITEQLGRQPSRIVACCGGGGLAAGLALACPDSAIVPVEPDGWDMVGQALKAGEIVAVGDDAPATICDALQPTTT
KPVNLAVLQGRSEPGVTVSDDEVRAAQRFAFSHLRLVVEPGGAAALAAAIAGKVSLDAHTVLMITGGNTDAASFAATIAA

Specific function: Acts On Both Serine And Threonine, And Properly Considered As A Hydroxy Amino Acid Deaminase. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11345492, Length=316, Percent_Identity=31.0126582278481, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI19923959, Length=323, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI33469958, Length=262, Percent_Identity=30.5343511450382, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1789505, Length=313, Percent_Identity=31.9488817891374, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1790207, Length=252, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1789235, Length=197, Percent_Identity=26.9035532994924, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17508781, Length=315, Percent_Identity=29.5238095238095, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71991565, Length=289, Percent_Identity=30.7958477508651, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17537387, Length=284, Percent_Identity=29.5774647887324, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6322631, Length=317, Percent_Identity=35.9621451104101, Blast_Score=182, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6320930, Length=262, Percent_Identity=35.8778625954198, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6319788, Length=298, Percent_Identity=26.8456375838926, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21355833, Length=136, Percent_Identity=32.3529411764706, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19

Molecular weight: Translated: 33119; Mature: 33119

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYHGKMIRKPTRDGVLEAAAKIADILPPTPLLPVEIDGTNCWVKAENLQPVGAFKIRGA
CCCCCCHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCCEEEECCH
WHRLSAMSEEERSRGVVAVSSGNHARGVAWAARRLGIAATIVMPRDAPKVKLEATKALGA
HHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHCCEEEEEEECCCCCCEEEEEHHCCCC
EVVLYERPGEDRDEVAATLIAERGGTLVHAFGDPWVIEGQGSAGIEITEQLGRQPSRIVA
EEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCCEEHHHHHCCCCCCEEE
CCGGGGLAAGLALACPDSAIVPVEPDGWDMVGQALKAGEIVAVGDDAPATICDALQPTTT
EECCCCHHHCEEEECCCCCEEEECCCCHHHHHHHHCCCCEEEECCCCCHHHHHCCCCCCC
KPVNLAVLQGRSEPGVTVSDDEVRAAQRFAFSHLRLVVEPGGAAALAAAIAGKVSLDAHT
CCEEEEEEECCCCCCCEECCHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHCCEEECCEE
VLMITGGNTDAASFAATIAA
EEEEECCCCCHHHHEEEECC
>Mature Secondary Structure
MRYHGKMIRKPTRDGVLEAAAKIADILPPTPLLPVEIDGTNCWVKAENLQPVGAFKIRGA
CCCCCCHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCCEEEECCH
WHRLSAMSEEERSRGVVAVSSGNHARGVAWAARRLGIAATIVMPRDAPKVKLEATKALGA
HHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHCCEEEEEEECCCCCCEEEEEHHCCCC
EVVLYERPGEDRDEVAATLIAERGGTLVHAFGDPWVIEGQGSAGIEITEQLGRQPSRIVA
EEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCCEEHHHHHCCCCCCEEE
CCGGGGLAAGLALACPDSAIVPVEPDGWDMVGQALKAGEIVAVGDDAPATICDALQPTTT
EECCCCHHHCEEEECCCCCEEEECCCCHHHHHHHHCCCCEEEECCCCCHHHHHCCCCCCC
KPVNLAVLQGRSEPGVTVSDDEVRAAQRFAFSHLRLVVEPGGAAALAAAIAGKVSLDAHT
CCEEEEEEECCCCCCCEECCHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHCCEEECCEE
VLMITGGNTDAASFAATIAA
EEEEECCCCCHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} 40 {L-Ser}} 5 {L-Ser}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = 2-oxobutanoate + NH3

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]