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The map label for this gene is otsB [H]

Identifier: 85373763

GI number: 85373763

Start: 967700

End: 968431

Strand: Direct

Name: otsB [H]

Synonym: ELI_04680

Alternate gene names: 85373763

Gene position: 967700-968431 (Clockwise)

Preceding gene: 85373762

Following gene: 85373764

Centisome position: 31.7

GC content: 64.48

Gene sequence:

>732_bases
ATGTTGCAGCGCAGCACTCTCCCACCGCCGCCGCCTCTGGCCGAACTGACCGAGCACGCCTTGTTCGTCGATTTCGATGG
AACGCTGGTGAAGATTGCCGAGGGTCCCGACAAAATCGACGTCGCCAAGGACCTTGGATCGCGGCTGGCGCGGCTCGCCG
CGAGGCTCGGCCAGCGGCTTGCCGTCGTGACAGGCCGCAGCCTCGAAAATCTCGAGAGTTTCCTCGACACCGCAGACTTT
TATCGTGCGGGTTCGCATGGTGCGCATGTGGTCGCACCAAATGGCGCGATGTTGCGGGAGGCGCAGGCTCTGCCGGATGA
GGTCATCGCAACGCTCAAGCGCTTCGCCGCTGAGCGTGGTCTGCTCTATGAGCGCAAAACCCACGGCGGTGCTATCCACT
ACCGGGCCGAGCCATCGCAGAAGCGGGCAGTGCAGGACATCGCCGCGTCTGTGGCGAGCGAACACGGCCTTTCGGCCAAG
CATGGCAAGTGCGTCGTCGAGCTGGTCTGGCCAGGCGCCGACAAGGGCGGGGCGGTACGATTGCTCGCCGAACGCGCGCC
TTTCGCCGGAGCCAGACCCGTCTTCATCGGTGACGATGTTACCGACGAAGACGGCATGGCCGCCGCCGCCGCGCTGGGCG
GTTTCGGCATCGCTGTCGGCGAGCGCCCGAGCGACAACGCCATCTATCACCTGAATTCTGTGAAAGACGTCCACGCATGG
CTGAAGCTGTAA

Upstream 100 bases:

>100_bases
ACGAGATGACGCGGCGTACCGACCCTCTAGTCGATCCTGCATAGGTATCCTTCCGCCGCTCGCCTTGCACCGCGCTCGCA
AGCCGCTATGCGTGTCGCCA

Downstream 100 bases:

>100_bases
TGTCCGACCTGCAATTGTGGCCGATCGGCAATTGCCAGGTCTCCGGCCTGATCGACCGGACGGGCGCGCTGGTCTGGGGC
TGTGTGCCGCGGGTCGATGG

Product: trehalose-phosphatase

Products: NA

Alternate protein names: TPP; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MLQRSTLPPPPPLAELTEHALFVDFDGTLVKIAEGPDKIDVAKDLGSRLARLAARLGQRLAVVTGRSLENLESFLDTADF
YRAGSHGAHVVAPNGAMLREAQALPDEVIATLKRFAAERGLLYERKTHGGAIHYRAEPSQKRAVQDIAASVASEHGLSAK
HGKCVVELVWPGADKGGAVRLLAERAPFAGARPVFIGDDVTDEDGMAAAAALGGFGIAVGERPSDNAIYHLNSVKDVHAW
LKL

Sequences:

>Translated_243_residues
MLQRSTLPPPPPLAELTEHALFVDFDGTLVKIAEGPDKIDVAKDLGSRLARLAARLGQRLAVVTGRSLENLESFLDTADF
YRAGSHGAHVVAPNGAMLREAQALPDEVIATLKRFAAERGLLYERKTHGGAIHYRAEPSQKRAVQDIAASVASEHGLSAK
HGKCVVELVWPGADKGGAVRLLAERAPFAGARPVFIGDDVTDEDGMAAAAALGGFGIAVGERPSDNAIYHLNSVKDVHAW
LKL
>Mature_243_residues
MLQRSTLPPPPPLAELTEHALFVDFDGTLVKIAEGPDKIDVAKDLGSRLARLAARLGQRLAVVTGRSLENLESFLDTADF
YRAGSHGAHVVAPNGAMLREAQALPDEVIATLKRFAAERGLLYERKTHGGAIHYRAEPSQKRAVQDIAASVASEHGLSAK
HGKCVVELVWPGADKGGAVRLLAERAPFAGARPVFIGDDVTDEDGMAAAAALGGFGIAVGERPSDNAIYHLNSVKDVHAW
LKL

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=240, Percent_Identity=33.75, Blast_Score=117, Evalue=7e-28,
Organism=Drosophila melanogaster, GI19920676, Length=235, Percent_Identity=26.8085106382979, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI221473368, Length=207, Percent_Identity=29.4685990338164, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI20129307, Length=207, Percent_Identity=29.4685990338164, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24582490, Length=207, Percent_Identity=29.4685990338164, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI20129309, Length=249, Percent_Identity=26.5060240963855, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 25850; Mature: 25850

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQRSTLPPPPPLAELTEHALFVDFDGTLVKIAEGPDKIDVAKDLGSRLARLAARLGQRL
CCCCCCCCCCCCHHHHHCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCEE
AVVTGRSLENLESFLDTADFYRAGSHGAHVVAPNGAMLREAQALPDEVIATLKRFAAERG
EEECCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHCCHHHHHHHHHHHHHHCC
LLYERKTHGGAIHYRAEPSQKRAVQDIAASVASEHGLSAKHGKCVVELVWPGADKGGAVR
CEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCEEE
LLAERAPFAGARPVFIGDDVTDEDGMAAAAALGGFGIAVGERPSDNAIYHLNSVKDVHAW
EEECCCCCCCCCCEEECCCCCCCCCHHHHHHHCCCEEEECCCCCCCEEEEECCHHHHHHH
LKL
HCC
>Mature Secondary Structure
MLQRSTLPPPPPLAELTEHALFVDFDGTLVKIAEGPDKIDVAKDLGSRLARLAARLGQRL
CCCCCCCCCCCCHHHHHCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCEE
AVVTGRSLENLESFLDTADFYRAGSHGAHVVAPNGAMLREAQALPDEVIATLKRFAAERG
EEECCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHCCHHHHHHHHHHHHHHCC
LLYERKTHGGAIHYRAEPSQKRAVQDIAASVASEHGLSAKHGKCVVELVWPGADKGGAVR
CEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCEEE
LLAERAPFAGARPVFIGDDVTDEDGMAAAAALGGFGIAVGERPSDNAIYHLNSVKDVHAW
EEECCCCCCCCCCEEECCCCCCCCCHHHHHHHCCCEEEECCCCCCCEEEEECCHHHHHHH
LKL
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]