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The map label for this gene is paaF [C]

Identifier: 85373760

GI number: 85373760

Start: 964643

End: 965446

Strand: Reverse

Name: paaF [C]

Synonym: ELI_04665

Alternate gene names: 85373760

Gene position: 965446-964643 (Counterclockwise)

Preceding gene: 85373768

Following gene: 85373759

Centisome position: 31.63

GC content: 63.31

Gene sequence:

>804_bases
GTGGGCGATTTCGTGAAACTGGAAAAGCACGGCAAGGTCGCAGTGCTGACGCTCAATAGCCCGGAGACATTGAACGCCAT
CGGCACGCAGGAGGACTGCGAGGATGTGATCGCTGCGCTCCACGCCATTGGCGAAGATGCGGGCGTGTCGTGCGCAATCC
TCACCGGCACCGGCAAGGGATTCTCCGCCGGCGGCAATATCAAGGCGATGAAGGAAAAGTCCGGCATCGGACCGCGCGAG
AAACCAGACGAGACGCGCAGCAATTACCGCCGCGGCGTCCAGCGCATCACCCGCGCCTTCCTCGATTGCGAGGTCCCGCT
GATCGCGGCGGTCAACGGTCATGCTGTGGGCCTCGGCTGCGACCTCGCCTGCCTGTGCGACATCCGCATCTCCGCAGAAA
GCGCGAAATATTCCGCCAGCTTCATCAAGGTCGGGATCGTCCCCGGCGACGGCGGCGCATGGTCGCTCCAGCGCGTGGTC
GGCTATTCTCAGGCGGCAGAGATGTTCTTGACCGGCGATCGCTATTCCGCCGAAGAGGCCAAGAGCTTCGGCCTTGTCAG
CCGTGTCGTGCCCGACGACCAAATGCTCGACACCGCGATGGAGCTTGCCGAACGCATCGCCGCCAATCCGGCACGCTCAC
TGCGCCTGACCAAGCGCCTGCTCCGCGAAGCTCAGCATAGCCGCATGTCGGATATTCTCGAACTCAGCGCGGCCTATCAG
GCCCTCGCCCACACGACCGACGACCACGGCGAAGCTGTCGACGCTTTCCTTGAAAAGCGCGAACCCAACTTCACTGGTGG
CTAG

Upstream 100 bases:

>100_bases
TTGATGTGCCAATCGTTGGGCAAGAGCAAGGGGGCACAAAGGCTGGCCTGCGCGCGCCGCCGTGCTAGGGATGCGCGAAA
GCTATAGGAGGAGAGGCCCG

Downstream 100 bases:

>100_bases
CTGGTATCAGCAAGCCGTTTCGACACTCTGACACCAAGCATCAGGCACCGCGCCGGGAGACACAATGAGCGATCAATCCG
AGTGGGTACAGCGGCCAGCG

Product: putative enoyl-CoA hydratase/isomerase family protein

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MGDFVKLEKHGKVAVLTLNSPETLNAIGTQEDCEDVIAALHAIGEDAGVSCAILTGTGKGFSAGGNIKAMKEKSGIGPRE
KPDETRSNYRRGVQRITRAFLDCEVPLIAAVNGHAVGLGCDLACLCDIRISAESAKYSASFIKVGIVPGDGGAWSLQRVV
GYSQAAEMFLTGDRYSAEEAKSFGLVSRVVPDDQMLDTAMELAERIAANPARSLRLTKRLLREAQHSRMSDILELSAAYQ
ALAHTTDDHGEAVDAFLEKREPNFTGG

Sequences:

>Translated_267_residues
MGDFVKLEKHGKVAVLTLNSPETLNAIGTQEDCEDVIAALHAIGEDAGVSCAILTGTGKGFSAGGNIKAMKEKSGIGPRE
KPDETRSNYRRGVQRITRAFLDCEVPLIAAVNGHAVGLGCDLACLCDIRISAESAKYSASFIKVGIVPGDGGAWSLQRVV
GYSQAAEMFLTGDRYSAEEAKSFGLVSRVVPDDQMLDTAMELAERIAANPARSLRLTKRLLREAQHSRMSDILELSAAYQ
ALAHTTDDHGEAVDAFLEKREPNFTGG
>Mature_266_residues
GDFVKLEKHGKVAVLTLNSPETLNAIGTQEDCEDVIAALHAIGEDAGVSCAILTGTGKGFSAGGNIKAMKEKSGIGPREK
PDETRSNYRRGVQRITRAFLDCEVPLIAAVNGHAVGLGCDLACLCDIRISAESAKYSASFIKVGIVPGDGGAWSLQRVVG
YSQAAEMFLTGDRYSAEEAKSFGLVSRVVPDDQMLDTAMELAERIAANPARSLRLTKRLLREAQHSRMSDILELSAAYQA
LAHTTDDHGEAVDAFLEKREPNFTGG

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=269, Percent_Identity=36.4312267657993, Blast_Score=154, Evalue=6e-38,
Organism=Homo sapiens, GI70995211, Length=261, Percent_Identity=31.4176245210728, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI45643119, Length=217, Percent_Identity=27.6497695852535, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI260275230, Length=217, Percent_Identity=27.6497695852535, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI260274832, Length=217, Percent_Identity=27.6497695852535, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI31542718, Length=213, Percent_Identity=27.2300469483568, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI4502327, Length=266, Percent_Identity=28.9473684210526, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI68989263, Length=222, Percent_Identity=27.4774774774775, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI116235487, Length=235, Percent_Identity=27.2340425531915, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI62530384, Length=220, Percent_Identity=27.7272727272727, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI37594469, Length=195, Percent_Identity=27.6923076923077, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI37594471, Length=195, Percent_Identity=27.6923076923077, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI20127408, Length=183, Percent_Identity=27.8688524590164, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI295842266, Length=218, Percent_Identity=26.605504587156, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=260, Percent_Identity=34.6153846153846, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1787660, Length=266, Percent_Identity=36.8421052631579, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI221142681, Length=275, Percent_Identity=32.7272727272727, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788597, Length=254, Percent_Identity=28.740157480315, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI87082183, Length=268, Percent_Identity=27.2388059701493, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1788682, Length=214, Percent_Identity=29.9065420560748, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790281, Length=194, Percent_Identity=26.8041237113402, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=256, Percent_Identity=36.328125, Blast_Score=140, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17554946, Length=265, Percent_Identity=32.8301886792453, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17535521, Length=257, Percent_Identity=27.2373540856031, Blast_Score=114, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17560910, Length=207, Percent_Identity=29.4685990338164, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=272, Percent_Identity=27.5735294117647, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17549921, Length=219, Percent_Identity=25.5707762557078, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=271, Percent_Identity=27.3062730627306, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17540714, Length=210, Percent_Identity=26.6666666666667, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=230, Percent_Identity=28.695652173913, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17558304, Length=218, Percent_Identity=28.4403669724771, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25144276, Length=188, Percent_Identity=27.1276595744681, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508953, Length=188, Percent_Identity=27.1276595744681, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508951, Length=188, Percent_Identity=27.1276595744681, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20129971, Length=266, Percent_Identity=35.7142857142857, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24653477, Length=266, Percent_Identity=35.7142857142857, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24653139, Length=261, Percent_Identity=29.5019157088123, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24654903, Length=217, Percent_Identity=27.1889400921659, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19922422, Length=264, Percent_Identity=27.6515151515151, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21357171, Length=246, Percent_Identity=28.0487804878049, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19920382, Length=259, Percent_Identity=29.7297297297297, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24650670, Length=259, Percent_Identity=27.027027027027, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28527; Mature: 28395

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDFVKLEKHGKVAVLTLNSPETLNAIGTQEDCEDVIAALHAIGEDAGVSCAILTGTGKG
CCCCEEEECCCCEEEEEECCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCC
FSAGGNIKAMKEKSGIGPREKPDETRSNYRRGVQRITRAFLDCEVPLIAAVNGHAVGLGC
CCCCCCEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEECC
DLACLCDIRISAESAKYSASFIKVGIVPGDGGAWSLQRVVGYSQAAEMFLTGDRYSAEEA
CEEEEEEEEECCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHEEECCCCCHHHH
KSFGLVSRVVPDDQMLDTAMELAERIAANPARSLRLTKRLLREAQHSRMSDILELSAAYQ
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAHTTDDHGEAVDAFLEKREPNFTGG
HHHHCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GDFVKLEKHGKVAVLTLNSPETLNAIGTQEDCEDVIAALHAIGEDAGVSCAILTGTGKG
CCCEEEECCCCEEEEEECCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCC
FSAGGNIKAMKEKSGIGPREKPDETRSNYRRGVQRITRAFLDCEVPLIAAVNGHAVGLGC
CCCCCCEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEECC
DLACLCDIRISAESAKYSASFIKVGIVPGDGGAWSLQRVVGYSQAAEMFLTGDRYSAEEA
CEEEEEEEEECCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHEEECCCCCHHHH
KSFGLVSRVVPDDQMLDTAMELAERIAANPARSLRLTKRLLREAQHSRMSDILELSAAYQ
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAHTTDDHGEAVDAFLEKREPNFTGG
HHHHCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA