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The map label for this gene is alsS [H]

Identifier: 85373722

GI number: 85373722

Start: 926001

End: 927641

Strand: Direct

Name: alsS [H]

Synonym: ELI_04475

Alternate gene names: 85373722

Gene position: 926001-927641 (Clockwise)

Preceding gene: 85373720

Following gene: 85373723

Centisome position: 30.34

GC content: 58.32

Gene sequence:

>1641_bases
ATGAAAGCCTCTGACTTGTTTATCGCTGCATTAGAGGCTGAAAAGGTGGAATACATCTTTGCCGTTCCCGGCGAAGAAAA
CCTCGACCTGCTGGAATCCCTGCGCACGTCCTCAATCGAATTGGTATTGACGCGCCATGAGCAAGGTGCGGGCTTTATGG
CGGCGACCTATGGCCGTCTGACCGGCAAGGCGGGGGTTTGCCTCGCAACTCTCGGGCCTGGTGCGACGAACCTGACAACT
CCGGCGGCCTATGCCGCGCTCGGGGCCTTTCCGCTCATCATCATCACCGGGCAGAAACCGATAAAGACATCGAAACAGGC
GCAGTTCCAAATTGTCGACGTTGTTTCGCTGTTTACTCCGCTATGCAAGGCTTCGACCCAGATCGTGAACGGCAATCGCA
TCCCCTCGCTCGTTCGCGAAGGTTTCCGACTTGCTCAGGAAGAAAGACCGGGCGCTGTGCTGCTCGAGCTTCCGGAAGAT
ATTGCTGAAGAGGAAACGATCGAACCGGTCATACCGCCGCATCAAAGGCGTTATGCGGTCGCCGGAGATGCGGCGCTCGA
CGAAGCGGCCGAGCGGATCATCGCGGCTGAGCGGCCACTGCTGCTAATCGGAGCCGGAGCAAACCGCCGCCGCGCTTCGA
AGGCGCTGCGGAAATTCGTGTCCGATACCGGTTTTCCCTTTTTCGACACCCAGATGGGCAAGGGCGTGGTCGACGAAGAT
AGCGAACTCTATCTGGGCACCGCGGCCCTATCTTCGGACGATTATGTTCACTGCGCGATCGAGAAGGCCGATCTGATCGT
CAATATCGGTCACGACGTGGTGGAGAAGCCGCCATTCTTCATGGAGCCGGACGGTCAGACTGTCATTCATATCAATTACA
AGGCGGCCGAAGTCGATCAGGTCTATTTCCCGCAGCTGGAAGTCATCGGTGACATTGCCGGTTCGGTCGAGCGGCTGGGA
AACCGTTTGGATGGCAACTTGCAGTGTGATCGCAGCTACTACACCGCCCTGCATCACGAGATTGCTTCGCACATTTCCGA
AACCAGCGACGACGACCGTTTTCCAATGGTTCCCCAACGCGTCGTTGCCGACGTTCGCAGCGTAATGGCACGCGATGATA
TCGTCGCGCTCGACAACGGCATCTACAAGATCTGGTTCGCTAGAAACTACATCGCTCATGAGCCTGGCACCATTCTGCTC
GACAATGCATTGGCGACGATGGGCGCGGGCCTTCCATCCGCCATGATGGCGGCGATTCTCACGCCCGGGCGCAGGGTTCT
CGCGGTGTGCGGCGATGGCGGGTTCATGATGAACTCGCAGGAACTGGAAACGGCCGTCAGGCTTGGCCTGAACCTCGTCG
TGCTCATCCTGAACGACGCGGCATACGGCATGATCCGGTGGAAGCAGGGCCAGCTCGGCTTTCCGGACTACGGCCTGACC
TTTTCCAATCCCGACTTTGTCACTTATGCCAAGAGCTATGGAGCGACCGGCCACCGTGTCGAGCGAAGCGCGGATCTGGT
TTCGGTCCTGAACGCCGCATTCGAGGCTGGCGGCGTGCACCTTGTCGACCTGCCCGTCGACTATTCCGAAAACAAGAAAG
TGCTGATCGACGAACTCGGCGCGAAGGTGTGCGAGCTATGA

Upstream 100 bases:

>100_bases
CGAATGAGGCGAGGCTCCTTGCGTCTGTGACTCTCTTTGCGGTTGTAGTGAGAAAGCATTGCCGTAGGGCATTCATGCTT
CTGAAAAGGGAATATCGAAA

Downstream 100 bases:

>100_bases
AAACGATCGTTCACAATCCCTTCGACCGCTCGCCGATTGCAGAAGTGGCGCTTGTCGACTGGGCTCAAATCGATGAATGG
CTGAATGAAGCGCAGTTGCT

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MKASDLFIAALEAEKVEYIFAVPGEENLDLLESLRTSSIELVLTRHEQGAGFMAATYGRLTGKAGVCLATLGPGATNLTT
PAAYAALGAFPLIIITGQKPIKTSKQAQFQIVDVVSLFTPLCKASTQIVNGNRIPSLVREGFRLAQEERPGAVLLELPED
IAEEETIEPVIPPHQRRYAVAGDAALDEAAERIIAAERPLLLIGAGANRRRASKALRKFVSDTGFPFFDTQMGKGVVDED
SELYLGTAALSSDDYVHCAIEKADLIVNIGHDVVEKPPFFMEPDGQTVIHINYKAAEVDQVYFPQLEVIGDIAGSVERLG
NRLDGNLQCDRSYYTALHHEIASHISETSDDDRFPMVPQRVVADVRSVMARDDIVALDNGIYKIWFARNYIAHEPGTILL
DNALATMGAGLPSAMMAAILTPGRRVLAVCGDGGFMMNSQELETAVRLGLNLVVLILNDAAYGMIRWKQGQLGFPDYGLT
FSNPDFVTYAKSYGATGHRVERSADLVSVLNAAFEAGGVHLVDLPVDYSENKKVLIDELGAKVCEL

Sequences:

>Translated_546_residues
MKASDLFIAALEAEKVEYIFAVPGEENLDLLESLRTSSIELVLTRHEQGAGFMAATYGRLTGKAGVCLATLGPGATNLTT
PAAYAALGAFPLIIITGQKPIKTSKQAQFQIVDVVSLFTPLCKASTQIVNGNRIPSLVREGFRLAQEERPGAVLLELPED
IAEEETIEPVIPPHQRRYAVAGDAALDEAAERIIAAERPLLLIGAGANRRRASKALRKFVSDTGFPFFDTQMGKGVVDED
SELYLGTAALSSDDYVHCAIEKADLIVNIGHDVVEKPPFFMEPDGQTVIHINYKAAEVDQVYFPQLEVIGDIAGSVERLG
NRLDGNLQCDRSYYTALHHEIASHISETSDDDRFPMVPQRVVADVRSVMARDDIVALDNGIYKIWFARNYIAHEPGTILL
DNALATMGAGLPSAMMAAILTPGRRVLAVCGDGGFMMNSQELETAVRLGLNLVVLILNDAAYGMIRWKQGQLGFPDYGLT
FSNPDFVTYAKSYGATGHRVERSADLVSVLNAAFEAGGVHLVDLPVDYSENKKVLIDELGAKVCEL
>Mature_546_residues
MKASDLFIAALEAEKVEYIFAVPGEENLDLLESLRTSSIELVLTRHEQGAGFMAATYGRLTGKAGVCLATLGPGATNLTT
PAAYAALGAFPLIIITGQKPIKTSKQAQFQIVDVVSLFTPLCKASTQIVNGNRIPSLVREGFRLAQEERPGAVLLELPED
IAEEETIEPVIPPHQRRYAVAGDAALDEAAERIIAAERPLLLIGAGANRRRASKALRKFVSDTGFPFFDTQMGKGVVDED
SELYLGTAALSSDDYVHCAIEKADLIVNIGHDVVEKPPFFMEPDGQTVIHINYKAAEVDQVYFPQLEVIGDIAGSVERLG
NRLDGNLQCDRSYYTALHHEIASHISETSDDDRFPMVPQRVVADVRSVMARDDIVALDNGIYKIWFARNYIAHEPGTILL
DNALATMGAGLPSAMMAAILTPGRRVLAVCGDGGFMMNSQELETAVRLGLNLVVLILNDAAYGMIRWKQGQLGFPDYGLT
FSNPDFVTYAKSYGATGHRVERSADLVSVLNAAFEAGGVHLVDLPVDYSENKKVLIDELGAKVCEL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=543, Percent_Identity=25.5985267034991, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI21361361, Length=561, Percent_Identity=24.9554367201426, Blast_Score=124, Evalue=3e-28,
Organism=Escherichia coli, GI1790104, Length=539, Percent_Identity=28.0148423005566, Blast_Score=205, Evalue=7e-54,
Organism=Escherichia coli, GI87081685, Length=551, Percent_Identity=27.7676950998185, Blast_Score=199, Evalue=5e-52,
Organism=Escherichia coli, GI1786717, Length=536, Percent_Identity=26.4925373134328, Blast_Score=188, Evalue=1e-48,
Organism=Escherichia coli, GI1788716, Length=471, Percent_Identity=26.1146496815287, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1787096, Length=535, Percent_Identity=24.4859813084112, Blast_Score=119, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17531301, Length=541, Percent_Identity=25.3234750462107, Blast_Score=141, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17531299, Length=541, Percent_Identity=25.3234750462107, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17542570, Length=554, Percent_Identity=24.5487364620939, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323755, Length=557, Percent_Identity=27.4685816876122, Blast_Score=200, Evalue=6e-52,
Organism=Saccharomyces cerevisiae, GI6320816, Length=492, Percent_Identity=22.7642276422764, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6321524, Length=494, Percent_Identity=22.2672064777328, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19922626, Length=486, Percent_Identity=26.3374485596708, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59225; Mature: 59225

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASDLFIAALEAEKVEYIFAVPGEENLDLLESLRTSSIELVLTRHEQGAGFMAATYGRL
CCCCCEEEEEEECCCEEEEEECCCCCCHHHHHHHCCCCEEEEEEECCCCCCEEEEHHHHC
TGKAGVCLATLGPGATNLTTPAAYAALGAFPLIIITGQKPIKTSKQAQFQIVDVVSLFTP
CCCCCEEEEECCCCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCEEHHHHHHHHHH
LCKASTQIVNGNRIPSLVREGFRLAQEERPGAVLLELPEDIAEEETIEPVIPPHQRRYAV
HHHCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCCCCCCCCCCCEEEE
AGDAALDEAAERIIAAERPLLLIGAGANRRRASKALRKFVSDTGFPFFDTQMGKGVVDED
ECCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEHHCCCCCCCCC
SELYLGTAALSSDDYVHCAIEKADLIVNIGHDVVEKPPFFMEPDGQTVIHINYKAAEVDQ
CCEEEEEEEECCCCEEEEEEECCEEEEECCHHHHCCCCCEECCCCCEEEEEEEECCCCCC
VYFPQLEVIGDIAGSVERLGNRLDGNLQCDRSYYTALHHEIASHISETSDDDRFPMVPQR
CCCCHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
VVADVRSVMARDDIVALDNGIYKIWFARNYIAHEPGTILLDNALATMGAGLPSAMMAAIL
HHHHHHHHHHCCCEEEECCCEEEEEEECCEECCCCCCEEECCHHHHHCCCCHHHHHHHHH
TPGRRVLAVCGDGGFMMNSQELETAVRLGLNLVVLILNDAAYGMIRWKQGQLGFPDYGLT
CCCCEEEEEECCCCEEECHHHHHHHHHHCCEEEEEEECCCCEEEEEEECCCCCCCCCCCC
FSNPDFVTYAKSYGATGHRVERSADLVSVLNAAFEAGGVHLVDLPVDYSENKKVLIDELG
CCCCCEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEHHHHH
AKVCEL
HHHHCC
>Mature Secondary Structure
MKASDLFIAALEAEKVEYIFAVPGEENLDLLESLRTSSIELVLTRHEQGAGFMAATYGRL
CCCCCEEEEEEECCCEEEEEECCCCCCHHHHHHHCCCCEEEEEEECCCCCCEEEEHHHHC
TGKAGVCLATLGPGATNLTTPAAYAALGAFPLIIITGQKPIKTSKQAQFQIVDVVSLFTP
CCCCCEEEEECCCCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCEEHHHHHHHHHH
LCKASTQIVNGNRIPSLVREGFRLAQEERPGAVLLELPEDIAEEETIEPVIPPHQRRYAV
HHHCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCCCCCCCCCCCEEEE
AGDAALDEAAERIIAAERPLLLIGAGANRRRASKALRKFVSDTGFPFFDTQMGKGVVDED
ECCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEHHCCCCCCCCC
SELYLGTAALSSDDYVHCAIEKADLIVNIGHDVVEKPPFFMEPDGQTVIHINYKAAEVDQ
CCEEEEEEEECCCCEEEEEEECCEEEEECCHHHHCCCCCEECCCCCEEEEEEEECCCCCC
VYFPQLEVIGDIAGSVERLGNRLDGNLQCDRSYYTALHHEIASHISETSDDDRFPMVPQR
CCCCHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
VVADVRSVMARDDIVALDNGIYKIWFARNYIAHEPGTILLDNALATMGAGLPSAMMAAIL
HHHHHHHHHHCCCEEEECCCEEEEEEECCEECCCCCCEEECCHHHHHCCCCHHHHHHHHH
TPGRRVLAVCGDGGFMMNSQELETAVRLGLNLVVLILNDAAYGMIRWKQGQLGFPDYGLT
CCCCEEEEEECCCCEEECHHHHHHHHHHCCEEEEEEECCCCEEEEEEECCCCCCCCCCCC
FSNPDFVTYAKSYGATGHRVERSADLVSVLNAAFEAGGVHLVDLPVDYSENKKVLIDELG
CCCCCEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEHHHHH
AKVCEL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377; 10809684 [H]