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The map label for this gene is gmuE [H]

Identifier: 85373673

GI number: 85373673

Start: 874600

End: 875475

Strand: Reverse

Name: gmuE [H]

Synonym: ELI_04230

Alternate gene names: 85373673

Gene position: 875475-874600 (Counterclockwise)

Preceding gene: 85373674

Following gene: 85373672

Centisome position: 28.68

GC content: 59.47

Gene sequence:

>876_bases
ATGGGCAACGGCTTGCTTGGCGGGATCGAGGCAGGCGGCACCAAATTCGTGCTCGCAGTTGGCTCCTCCCCAACCGACAT
CCTTTCCCGTCATTCGATCCCGACGCGCAAGCCAGAGGACACCTTGGCAGAAGCCGCTGAGTGGTTCGCCAGCCAGGGTC
GTCTTGAGGCTCTGGGCATCGCCAGTTTTGGACCGGTCGATCTTGATCGCGATTCTCCCAGCTGGGGCAGCATCACCAAC
ACACCAAAGCCCGGCTGGTCGGGTTGCGCGCTGGCGAGCTTTTTCGGAAAGCGTTTCGGCGTACCAATCGGCTTCGACAC
CGATGTCAACGGCGCGGCACTGGCTGAATACCATTTTGGTGCCGGGCGAGACGCGTCCTCGCTAGCCTATGTTACCGTGG
GGACCGGCATCGGGGGCGGACTGGTCGTCGACGGAAAAGCACTTCATGGCGCTGCTCACCCGGAGATGGGGCACATCTTT
CCGCGCAGGCATACAAAGGATTTAGAGTTCGCAGGCACCTGTCCGCATCATGGAGATTGCCTCGAAGGTCTAGCATGCGG
ACCCGCGATACTCGCACGCTGGGGAAGCTCGCTCTCCGAATTGCCGCTCGACCATGTCGCTCACCGGGTAATAGCGGACT
ATCTGGCGCAGCTTTGCCACACGATATTCGCGACCACTTCGGCAGAAGTCATCGTGCTCGGAGGGGGAGTGATGGGTACA
CCCGGTCTTCTTGAAAAGGTGAACGCTCGCGCACGCCAACTAGATAAAGAGTATCTTCCGGGCGGACCAAAACACCTGCT
CGCTGCACCGTTGCTTGGACAAGATTCGGGCGCAGTTGGAGCATTACTCCTTGCAAAGATGACTAGCACCGATTGA

Upstream 100 bases:

>100_bases
TCACCGAAGAAGAAACCCGCAGAGCGCGTTTCGGAGGCACCTTTGCTCCGTTCGTGCAGATTGCATTGGATGGCGATAAA
GCGCGCCTGGTAGAAGGCTG

Downstream 100 bases:

>100_bases
ATTTTTTGAGGTGTCAACCTGCTCTCGGGACGAAGCGCATTCAATCCTAGCTTTGGGCCGACATCTTGTGAACAAGCAAT
TCGTCTCTGCGAACTCACCG

Product: fructokinase

Products: NA

Alternate protein names: Glucomannan utilization protein E [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MGNGLLGGIEAGGTKFVLAVGSSPTDILSRHSIPTRKPEDTLAEAAEWFASQGRLEALGIASFGPVDLDRDSPSWGSITN
TPKPGWSGCALASFFGKRFGVPIGFDTDVNGAALAEYHFGAGRDASSLAYVTVGTGIGGGLVVDGKALHGAAHPEMGHIF
PRRHTKDLEFAGTCPHHGDCLEGLACGPAILARWGSSLSELPLDHVAHRVIADYLAQLCHTIFATTSAEVIVLGGGVMGT
PGLLEKVNARARQLDKEYLPGGPKHLLAAPLLGQDSGAVGALLLAKMTSTD

Sequences:

>Translated_291_residues
MGNGLLGGIEAGGTKFVLAVGSSPTDILSRHSIPTRKPEDTLAEAAEWFASQGRLEALGIASFGPVDLDRDSPSWGSITN
TPKPGWSGCALASFFGKRFGVPIGFDTDVNGAALAEYHFGAGRDASSLAYVTVGTGIGGGLVVDGKALHGAAHPEMGHIF
PRRHTKDLEFAGTCPHHGDCLEGLACGPAILARWGSSLSELPLDHVAHRVIADYLAQLCHTIFATTSAEVIVLGGGVMGT
PGLLEKVNARARQLDKEYLPGGPKHLLAAPLLGQDSGAVGALLLAKMTSTD
>Mature_290_residues
GNGLLGGIEAGGTKFVLAVGSSPTDILSRHSIPTRKPEDTLAEAAEWFASQGRLEALGIASFGPVDLDRDSPSWGSITNT
PKPGWSGCALASFFGKRFGVPIGFDTDVNGAALAEYHFGAGRDASSLAYVTVGTGIGGGLVVDGKALHGAAHPEMGHIFP
RRHTKDLEFAGTCPHHGDCLEGLACGPAILARWGSSLSELPLDHVAHRVIADYLAQLCHTIFATTSAEVIVLGGGVMGTP
GLLEKVNARARQLDKEYLPGGPKHLLAAPLLGQDSGAVGALLLAKMTSTD

Specific function: Seems to be involved in the degradation of glucomannan [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

None

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 30065; Mature: 29934

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNGLLGGIEAGGTKFVLAVGSSPTDILSRHSIPTRKPEDTLAEAAEWFASQGRLEALGI
CCCCCCCCCCCCCEEEEEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEEC
ASFGPVDLDRDSPSWGSITNTPKPGWSGCALASFFGKRFGVPIGFDTDVNGAALAEYHFG
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCC
AGRDASSLAYVTVGTGIGGGLVVDGKALHGAAHPEMGHIFPRRHTKDLEFAGTCPHHGDC
CCCCCCCEEEEEECCCCCCCEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
LEGLACGPAILARWGSSLSELPLDHVAHRVIADYLAQLCHTIFATTSAEVIVLGGGVMGT
HHHHHCCHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCC
PGLLEKVNARARQLDKEYLPGGPKHLLAAPLLGQDSGAVGALLLAKMTSTD
CHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
GNGLLGGIEAGGTKFVLAVGSSPTDILSRHSIPTRKPEDTLAEAAEWFASQGRLEALGI
CCCCCCCCCCCCEEEEEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEEC
ASFGPVDLDRDSPSWGSITNTPKPGWSGCALASFFGKRFGVPIGFDTDVNGAALAEYHFG
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCC
AGRDASSLAYVTVGTGIGGGLVVDGKALHGAAHPEMGHIFPRRHTKDLEFAGTCPHHGDC
CCCCCCCEEEEEECCCCCCCEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
LEGLACGPAILARWGSSLSELPLDHVAHRVIADYLAQLCHTIFATTSAEVIVLGGGVMGT
HHHHHCCHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCC
PGLLEKVNARARQLDKEYLPGGPKHLLAAPLLGQDSGAVGALLLAKMTSTD
CHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]