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The map label for this gene is traD [H]

Identifier: 85373669

GI number: 85373669

Start: 866799

End: 869138

Strand: Direct

Name: traD [H]

Synonym: ELI_04210

Alternate gene names: 85373669

Gene position: 866799-869138 (Clockwise)

Preceding gene: 85373668

Following gene: 85373670

Centisome position: 28.4

GC content: 62.22

Gene sequence:

>2340_bases
ATGAAGCATAATCTCGTCAACTTCACCCGCGGCAGCCAGCTTCTGGGGCATTTCGGCTTCATGTTTGCTGCGGGCCTCAA
GGGGCCCCTGATCGTGACCTTCCTGGTCGTTCTCGGCCTGGTCTATTGGGACGTAAGCGGGGCGCTAGACGACTACCAGG
TCTACCTCCTGTGGATGCATGCCTATGCCTCTGGTTATGCCTTCATGGAGTTCGATCCGGACAAGCTTGTGAACCTCAAG
CTGGCCGATGGGAGCCTCGTCGCATTTCCCATTTCCGTCGTGACCGATTACCCGCCGATGCGCGAAGCGGTCGCGCTATT
CCGGAGTGCGGTGATCAGCGCTTTGTGGCTTGCCGGGTTCATTCTTGCGCCGCTCTTCGCGCTTTTCTGGTGGGTTGCCG
AACGCTTCGGCGAGAAGTCCAAACAGAAGAAGCATGTGCGCGGGGCGGAGCTTGCTACCTTGCGTGAACTTGACCGGGAG
ATCGCCGCGCACAACCGCAATGCCCGTGCCCGCGAGTATGGCGATGCGTTGGGCTGGCGCTGGCGTCTGGCCGGGTCGGC
TTCGCTGCGGGATGCGGGCTTCTATTCTCCCGCGCATCTGGCAGGGGTCAGCTGGCCTTGGCGCCTCGAGCAGAGCCACG
CGATGCTTGTTGGCACAACCGGGACAGGCAAGACCGTCGTCCTAACCGAGCTTACCAGGGAGATCCGTGAACGGGGCGAA
CGTGCGGTCATTTTCGATCTTACCGGGGCCTTTGTCGAAACCTTCTACGATCCCAAACGCGACATCATTCTCAACCCGCT
CGACGCGCGCTGTCCCGCATGGAGCGTGTTCAACGATTGCTCGACACGTGCCGAATTCCATGCCGCGGCGGAGTCCCTCG
TCCCGCACGATGGGGGCGGTGCCGAACAGTTCTGGGTGCTCGCCGCGCGCACGCTGTTCGTCGAGACCTGTCTCAAGCTC
GCCGAAGCTGGGAGGGGCACCAACGCCGCGCTCGCCCACGAACTCATGAACGCCGATCTCTCCGATCTGCACCGTCTCGT
CGAAGATACCATGGCCGGTCCCATCAGCACGCCGAGCGCGGCGCGCATGGCGGAATCGGTGCGTGCAGTGTTCAATGTCA
ACGCCAAGGCGCTCCAGATGCTGCCCGAAGACGGGACGCCCTTTTCGGTCCGCGAGTGGGTCAACGGGGCAGGTGAACCG
GGCTCTATCCTGTTTCTGTCCGCGCGCTACATCGACATGAGCGTGCTCTCGCAGCTGCTCACCCTGTGGCTCGATACCGC
GATCAATACGCTGATGGCGGGCCAGCGCTCGCGGGACATCAGGCTCTGGTTCCTGATCGACGAACTGGGCGCGCTGCATC
GCCTGCCTTCGCTCGAGAAGGGCCTCCAGACCGCGCGCAATTTCGGGGGCGCGATCGTCACCGGGATCCATGCCTTTGCC
AAGCTCAAGGATGTCTACGGCGAGAACATGGCCATGACCCTGTCCTCGCTTGCCCGAACCAAGCTGATCCTCGGCACAGC
AGATCGCGAGACCGCCACCTGGTGTTCCGATATCATTGGCCACCGCGAGGTTCGCGAGATGGAGGAAGGATACAGCTACG
GCTATAACAATGCGCGCGACGCGGTAAGCCTGACGCCCCGGCGCCAGGTCGTCCCACTGCTGCTTCCCGACGAGCTGATG
AAGCTCAAGAACCTACATGGTTTCATCAAGTTTCCCGAAGGATTTCCGGCGGCACCCGTGGTCCTCGAACCGCGCAACTG
GCCTCGGGTGGCCGAAGGCTTCATTCCCCGGGATATGCCGAAGTTACCACCGCAGACCCGGCACGCTGATGATAATGAGG
GAGCAGGGGGCGGTGCCGGAGCAGCCTCTGAAACAGGCGACAATGATGGTGATCCTCGCCGGATGAGGAATGCGCACGAT
CGCACCGACAGCGCTGAAAAGAAAGCTGACCAAGAAGAGAAAAAGGAACCTCGCCGGACCCGTCGCAGCAACAAGGTCGA
GCAGGCTCGACCGGATCAGACAAGGAAGGCTCGCGATCCGAATGCGCCAGGGAAAAGGGCGACATCGGACCCTCAGCGGC
CGGCTCAATCCGAACTCCCTCTGTCTTCGAAAGCTGAGGAGCACCGCGGCATGGAGCAACGGGCTGTGCCTTCCGCCAGA
TCCGATATTCCCGATCCCGCAGCGCATCAGCGAGCACAGGTCGCGCGCGGCAAGGCCGACCAGAGGCTCCAGGAAGAGCA
GCAGAAGTCGCTGCTTGGCGGTGCAGCGAAGCAGGGTCGCGATGCCGATCAGGGGCAGGACCATGGTCACGATTACGGGG
ACTTCGATCCGGACATGTGA

Upstream 100 bases:

>100_bases
CATCATCCCGATAGCACTTTGCGCGACAGAACGGTCGCTGCCTACCAGCGCCGGCTGGAGCGCCTTGGCATCGTTGCACC
CCGACCCATGGGAGGCGAGG

Downstream 100 bases:

>100_bases
CGCTTAACCGGGGAGGGGACGAGGGCCAAGAGAAAGTGATCCCAGCCCATGCTTTCCGTCGCCAATGTCCGCTCGCCTTC
GGCGGCGGCAAGCTACTTCG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 779; Mature: 779

Protein sequence:

>779_residues
MKHNLVNFTRGSQLLGHFGFMFAAGLKGPLIVTFLVVLGLVYWDVSGALDDYQVYLLWMHAYASGYAFMEFDPDKLVNLK
LADGSLVAFPISVVTDYPPMREAVALFRSAVISALWLAGFILAPLFALFWWVAERFGEKSKQKKHVRGAELATLRELDRE
IAAHNRNARAREYGDALGWRWRLAGSASLRDAGFYSPAHLAGVSWPWRLEQSHAMLVGTTGTGKTVVLTELTREIRERGE
RAVIFDLTGAFVETFYDPKRDIILNPLDARCPAWSVFNDCSTRAEFHAAAESLVPHDGGGAEQFWVLAARTLFVETCLKL
AEAGRGTNAALAHELMNADLSDLHRLVEDTMAGPISTPSAARMAESVRAVFNVNAKALQMLPEDGTPFSVREWVNGAGEP
GSILFLSARYIDMSVLSQLLTLWLDTAINTLMAGQRSRDIRLWFLIDELGALHRLPSLEKGLQTARNFGGAIVTGIHAFA
KLKDVYGENMAMTLSSLARTKLILGTADRETATWCSDIIGHREVREMEEGYSYGYNNARDAVSLTPRRQVVPLLLPDELM
KLKNLHGFIKFPEGFPAAPVVLEPRNWPRVAEGFIPRDMPKLPPQTRHADDNEGAGGGAGAASETGDNDGDPRRMRNAHD
RTDSAEKKADQEEKKEPRRTRRSNKVEQARPDQTRKARDPNAPGKRATSDPQRPAQSELPLSSKAEEHRGMEQRAVPSAR
SDIPDPAAHQRAQVARGKADQRLQEEQQKSLLGGAAKQGRDADQGQDHGHDYGDFDPDM

Sequences:

>Translated_779_residues
MKHNLVNFTRGSQLLGHFGFMFAAGLKGPLIVTFLVVLGLVYWDVSGALDDYQVYLLWMHAYASGYAFMEFDPDKLVNLK
LADGSLVAFPISVVTDYPPMREAVALFRSAVISALWLAGFILAPLFALFWWVAERFGEKSKQKKHVRGAELATLRELDRE
IAAHNRNARAREYGDALGWRWRLAGSASLRDAGFYSPAHLAGVSWPWRLEQSHAMLVGTTGTGKTVVLTELTREIRERGE
RAVIFDLTGAFVETFYDPKRDIILNPLDARCPAWSVFNDCSTRAEFHAAAESLVPHDGGGAEQFWVLAARTLFVETCLKL
AEAGRGTNAALAHELMNADLSDLHRLVEDTMAGPISTPSAARMAESVRAVFNVNAKALQMLPEDGTPFSVREWVNGAGEP
GSILFLSARYIDMSVLSQLLTLWLDTAINTLMAGQRSRDIRLWFLIDELGALHRLPSLEKGLQTARNFGGAIVTGIHAFA
KLKDVYGENMAMTLSSLARTKLILGTADRETATWCSDIIGHREVREMEEGYSYGYNNARDAVSLTPRRQVVPLLLPDELM
KLKNLHGFIKFPEGFPAAPVVLEPRNWPRVAEGFIPRDMPKLPPQTRHADDNEGAGGGAGAASETGDNDGDPRRMRNAHD
RTDSAEKKADQEEKKEPRRTRRSNKVEQARPDQTRKARDPNAPGKRATSDPQRPAQSELPLSSKAEEHRGMEQRAVPSAR
SDIPDPAAHQRAQVARGKADQRLQEEQQKSLLGGAAKQGRDADQGQDHGHDYGDFDPDM
>Mature_779_residues
MKHNLVNFTRGSQLLGHFGFMFAAGLKGPLIVTFLVVLGLVYWDVSGALDDYQVYLLWMHAYASGYAFMEFDPDKLVNLK
LADGSLVAFPISVVTDYPPMREAVALFRSAVISALWLAGFILAPLFALFWWVAERFGEKSKQKKHVRGAELATLRELDRE
IAAHNRNARAREYGDALGWRWRLAGSASLRDAGFYSPAHLAGVSWPWRLEQSHAMLVGTTGTGKTVVLTELTREIRERGE
RAVIFDLTGAFVETFYDPKRDIILNPLDARCPAWSVFNDCSTRAEFHAAAESLVPHDGGGAEQFWVLAARTLFVETCLKL
AEAGRGTNAALAHELMNADLSDLHRLVEDTMAGPISTPSAARMAESVRAVFNVNAKALQMLPEDGTPFSVREWVNGAGEP
GSILFLSARYIDMSVLSQLLTLWLDTAINTLMAGQRSRDIRLWFLIDELGALHRLPSLEKGLQTARNFGGAIVTGIHAFA
KLKDVYGENMAMTLSSLARTKLILGTADRETATWCSDIIGHREVREMEEGYSYGYNNARDAVSLTPRRQVVPLLLPDELM
KLKNLHGFIKFPEGFPAAPVVLEPRNWPRVAEGFIPRDMPKLPPQTRHADDNEGAGGGAGAASETGDNDGDPRRMRNAHD
RTDSAEKKADQEEKKEPRRTRRSNKVEQARPDQTRKARDPNAPGKRATSDPQRPAQSELPLSSKAEEHRGMEQRAVPSAR
SDIPDPAAHQRAQVARGKADQRLQEEQQKSLLGGAAKQGRDADQGQDHGHDYGDFDPDM

Specific function: TraD is a cell envelope protein. The function of traD is unknown but it is essential for DNA transfer. It may form or may be part of a non-specific pore for DNA export, or it may directly energizes DNA transport [H]

COG id: COG3505

COG function: function code U; Type IV secretory pathway, VirD4 components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To traD of plasmid F [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014128
- InterPro:   IPR019476
- InterPro:   IPR022585 [H]

Pfam domain/function: PF12615 TraD_N; PF10412 TrwB_AAD_bind [H]

EC number: NA

Molecular weight: Translated: 86233; Mature: 86233

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHNLVNFTRGSQLLGHFGFMFAAGLKGPLIVTFLVVLGLVYWDVSGALDDYQVYLLWMH
CCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AYASGYAFMEFDPDKLVNLKLADGSLVAFPISVVTDYPPMREAVALFRSAVISALWLAGF
HHHCCEEEEEECCCCEEEEEECCCCEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ILAPLFALFWWVAERFGEKSKQKKHVRGAELATLRELDREIAAHNRNARAREYGDALGWR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEE
WRLAGSASLRDAGFYSPAHLAGVSWPWRLEQSHAMLVGTTGTGKTVVLTELTREIRERGE
EEECCCCCCCCCCCCCCHHHCCCCCCCEECCCCEEEEEECCCCCEEHHHHHHHHHHHCCC
RAVIFDLTGAFVETFYDPKRDIILNPLDARCPAWSVFNDCSTRAEFHAAAESLVPHDGGG
EEEEEECHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
AEQFWVLAARTLFVETCLKLAEAGRGTNAALAHELMNADLSDLHRLVEDTMAGPISTPSA
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHH
ARMAESVRAVFNVNAKALQMLPEDGTPFSVREWVNGAGEPGSILFLSARYIDMSVLSQLL
HHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHH
TLWLDTAINTLMAGQRSRDIRLWFLIDELGALHRLPSLEKGLQTARNFGGAIVTGIHAFA
HHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHH
KLKDVYGENMAMTLSSLARTKLILGTADRETATWCSDIIGHREVREMEEGYSYGYNNARD
HHHHHHCCCHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AVSLTPRRQVVPLLLPDELMKLKNLHGFIKFPEGFPAAPVVLEPRNWPRVAEGFIPRDMP
CEECCCCCCEECEECCHHHHHHHHCCCEEECCCCCCCCCEEECCCCCCHHHHCCCCCCCC
KLPPQTRHADDNEGAGGGAGAASETGDNDGDPRRMRNAHDRTDSAEKKADQEEKKEPRRT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHCCHHHHHHHHHH
RRSNKVEQARPDQTRKARDPNAPGKRATSDPQRPAQSELPLSSKAEEHRGMEQRAVPSAR
HHHCCHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHCCCCHH
SDIPDPAAHQRAQVARGKADQRLQEEQQKSLLGGAAKQGRDADQGQDHGHDYGDFDPDM
CCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKHNLVNFTRGSQLLGHFGFMFAAGLKGPLIVTFLVVLGLVYWDVSGALDDYQVYLLWMH
CCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AYASGYAFMEFDPDKLVNLKLADGSLVAFPISVVTDYPPMREAVALFRSAVISALWLAGF
HHHCCEEEEEECCCCEEEEEECCCCEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ILAPLFALFWWVAERFGEKSKQKKHVRGAELATLRELDREIAAHNRNARAREYGDALGWR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEE
WRLAGSASLRDAGFYSPAHLAGVSWPWRLEQSHAMLVGTTGTGKTVVLTELTREIRERGE
EEECCCCCCCCCCCCCCHHHCCCCCCCEECCCCEEEEEECCCCCEEHHHHHHHHHHHCCC
RAVIFDLTGAFVETFYDPKRDIILNPLDARCPAWSVFNDCSTRAEFHAAAESLVPHDGGG
EEEEEECHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
AEQFWVLAARTLFVETCLKLAEAGRGTNAALAHELMNADLSDLHRLVEDTMAGPISTPSA
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHH
ARMAESVRAVFNVNAKALQMLPEDGTPFSVREWVNGAGEPGSILFLSARYIDMSVLSQLL
HHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHH
TLWLDTAINTLMAGQRSRDIRLWFLIDELGALHRLPSLEKGLQTARNFGGAIVTGIHAFA
HHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHH
KLKDVYGENMAMTLSSLARTKLILGTADRETATWCSDIIGHREVREMEEGYSYGYNNARD
HHHHHHCCCHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AVSLTPRRQVVPLLLPDELMKLKNLHGFIKFPEGFPAAPVVLEPRNWPRVAEGFIPRDMP
CEECCCCCCEECEECCHHHHHHHHCCCEEECCCCCCCCCEEECCCCCCHHHHCCCCCCCC
KLPPQTRHADDNEGAGGGAGAASETGDNDGDPRRMRNAHDRTDSAEKKADQEEKKEPRRT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHCCHHHHHHHHHH
RRSNKVEQARPDQTRKARDPNAPGKRATSDPQRPAQSELPLSSKAEEHRGMEQRAVPSAR
HHHCCHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHCCCCHH
SDIPDPAAHQRAQVARGKADQRLQEEQQKSLLGGAAKQGRDADQGQDHGHDYGDFDPDM
CCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2164585 [H]