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The map label for this gene is ilvG [H]

Identifier: 85373613

GI number: 85373613

Start: 810661

End: 812322

Strand: Reverse

Name: ilvG [H]

Synonym: ELI_03930

Alternate gene names: 85373613

Gene position: 812322-810661 (Counterclockwise)

Preceding gene: 85373615

Following gene: 85373611

Centisome position: 26.61

GC content: 64.86

Gene sequence:

>1662_bases
ATGACTCGATCCACCACTCCCGATGCCGCCCGCCTGCTCGTCGACTGCCTTGTCGAGCAGGGCTGCGACAGGGTCTTCAC
CGTCCCCGGCGAAAGCTTCCTGCAGGTGCTCGACGGGCTGCACCAACACGGCTCGATCGATGTCGTCACCTGCCGGCAGG
AAGGCGGCGTCGCCTTCATGGCCTGCGCCGACGGGGCGATGACCAACCGTCCGGGGGTCGCCTTCGTCACCCGCGGCCCG
GGCGCGACCAATGCCAGTATCGGCGTGCATGTCGCGTTGCAGGATTCGCAGCCGATGATCCTCTTCGTCGGCGATGTCGG
GCGCGATATGCGCGAACGCGAAGGCTTTCAGGAGATCGATTTTCTCGCCTTTTTCGGTCCGATCGCGAAATGGGCCGCGC
GGATCGACAGTGCCGACCGTATCCCGGAATTCGTAGCACGGGCCTATTCGGTTGCGATGTCGGGGCGTCCCGGGCCGGTG
GTGCTGGCGCTTCCCGAAGACATGCTCTCCGATACGACGACCGCGCGCCCTCGCCCGCAAGTTGCCCGACCGGCGCAAGC
GGTCTGTCCCGACGCGATGCAAGCGATGATGGCCATGATCGGCGATGCCGCCTCCCCCGTGGCGATCATCGGCGGCGCTG
GCTGGCACTCCAAGGCGCGCGAGCATTTCCAGTTGTTCGCCGAGCGGATCGGATTGCCCGTCGCGACCGCCTTTCGCCGG
CAGGATGCGATTTCTCCTGCTTCACCGGTCTACGCCGGGAACCTGGGATATGGTCCCAATCCCAAGCTGGTCGAGCGTGT
GAGGAATGCCGATCTGATTCTTGCGGTTGGCGCGAGGCTTGGGGAAGCGACGACCGAAGGTTATACCCTCATCACGCCCG
ATCACCCCGACCAGATGCTGATCCACGTCCATCCCGACCCGGACGAACTCGGCAGCGTCTATCGCACCGACCTGTCGCTA
TGCGCCGACATGGGCGAATTCGCCGAAGCCGCGGCGCTGTGGGAAGAAAATTCCGTCATTTCGTTCGACGCTGGCGCAGA
GGCGCATGCCGAGTGGGAGGCATGGGCCACCGCCACGCCGGGCGACTGGCAACTCGACCTTGCCCGCTGCGTCACCTTCA
TGCGCGACACGCTCCCAGCCGATACCATCATCTGCAACGGCGCGGGCAATTTCGCCGGTTGGTGGCACCGCTACTGGCGC
TACCGGGGCCATCCGACCCAGCTAGCCCCGACCTGCGGCGCGATGGGCTATGGCGTGCCAGCTGCGGTCGCGGCGGCGCG
GCGCTTCCCCAATCGGACCGTGGTGGCAATCGCGGGCGATGGCGATTTTCTGATGAACGGGCAGGAACTGGCGACCGCTG
CGCAATATAGTCTCAACCTGATGGTGATCATCGTCGACAACGGCGCTTACGGCACCATTCGGATGCACCAGGAACGGGAA
TTTCCTGAGCGGATTTCGGCCACCGAGCTGAAAAACCCCGACTTCGCTGCACTCGCAGAATCCTATGGCGGCTGGTCAGC
CCGTGCAAAAACGACCGACGAGTTCAAGACTGGGCTGGTAGAGGCCGAGGGACGCAGCGGCCTGCGGCTGATCCACTGTC
TGATCGATGTCGAGCAATTGGCCGCCAGCGGCGCGACAATCAGCGGGCTGCGCAGTCGCTAG

Upstream 100 bases:

>100_bases
CGGCCATTCCGGCCTGTCGCAGGATGGGACGTTAACTGTCCCTGTTCATGACGCGGTGCCGCGTTGTCGTCCTCGAAGGC
TGGCGCTAGAGAATCGGGCC

Downstream 100 bases:

>100_bases
CTTACCTTTCCCCGGCCAATTCCAACACTATATCCCATTCCTCGGGACGGACCTCGGCCACAGACAGCCGTGATAGTTTG
ACCAGTTCCATCTCCGTCAG

Product: thiamine pyrophosphate protein

Products: NA

Alternate protein names: AHAS-II; ALS-II; Acetohydroxy-acid synthase II large subunit [H]

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MTRSTTPDAARLLVDCLVEQGCDRVFTVPGESFLQVLDGLHQHGSIDVVTCRQEGGVAFMACADGAMTNRPGVAFVTRGP
GATNASIGVHVALQDSQPMILFVGDVGRDMREREGFQEIDFLAFFGPIAKWAARIDSADRIPEFVARAYSVAMSGRPGPV
VLALPEDMLSDTTTARPRPQVARPAQAVCPDAMQAMMAMIGDAASPVAIIGGAGWHSKAREHFQLFAERIGLPVATAFRR
QDAISPASPVYAGNLGYGPNPKLVERVRNADLILAVGARLGEATTEGYTLITPDHPDQMLIHVHPDPDELGSVYRTDLSL
CADMGEFAEAAALWEENSVISFDAGAEAHAEWEAWATATPGDWQLDLARCVTFMRDTLPADTIICNGAGNFAGWWHRYWR
YRGHPTQLAPTCGAMGYGVPAAVAAARRFPNRTVVAIAGDGDFLMNGQELATAAQYSLNLMVIIVDNGAYGTIRMHQERE
FPERISATELKNPDFAALAESYGGWSARAKTTDEFKTGLVEAEGRSGLRLIHCLIDVEQLAASGATISGLRSR

Sequences:

>Translated_553_residues
MTRSTTPDAARLLVDCLVEQGCDRVFTVPGESFLQVLDGLHQHGSIDVVTCRQEGGVAFMACADGAMTNRPGVAFVTRGP
GATNASIGVHVALQDSQPMILFVGDVGRDMREREGFQEIDFLAFFGPIAKWAARIDSADRIPEFVARAYSVAMSGRPGPV
VLALPEDMLSDTTTARPRPQVARPAQAVCPDAMQAMMAMIGDAASPVAIIGGAGWHSKAREHFQLFAERIGLPVATAFRR
QDAISPASPVYAGNLGYGPNPKLVERVRNADLILAVGARLGEATTEGYTLITPDHPDQMLIHVHPDPDELGSVYRTDLSL
CADMGEFAEAAALWEENSVISFDAGAEAHAEWEAWATATPGDWQLDLARCVTFMRDTLPADTIICNGAGNFAGWWHRYWR
YRGHPTQLAPTCGAMGYGVPAAVAAARRFPNRTVVAIAGDGDFLMNGQELATAAQYSLNLMVIIVDNGAYGTIRMHQERE
FPERISATELKNPDFAALAESYGGWSARAKTTDEFKTGLVEAEGRSGLRLIHCLIDVEQLAASGATISGLRSR
>Mature_552_residues
TRSTTPDAARLLVDCLVEQGCDRVFTVPGESFLQVLDGLHQHGSIDVVTCRQEGGVAFMACADGAMTNRPGVAFVTRGPG
ATNASIGVHVALQDSQPMILFVGDVGRDMREREGFQEIDFLAFFGPIAKWAARIDSADRIPEFVARAYSVAMSGRPGPVV
LALPEDMLSDTTTARPRPQVARPAQAVCPDAMQAMMAMIGDAASPVAIIGGAGWHSKAREHFQLFAERIGLPVATAFRRQ
DAISPASPVYAGNLGYGPNPKLVERVRNADLILAVGARLGEATTEGYTLITPDHPDQMLIHVHPDPDELGSVYRTDLSLC
ADMGEFAEAAALWEENSVISFDAGAEAHAEWEAWATATPGDWQLDLARCVTFMRDTLPADTIICNGAGNFAGWWHRYWRY
RGHPTQLAPTCGAMGYGVPAAVAAARRFPNRTVVAIAGDGDFLMNGQELATAAQYSLNLMVIIVDNGAYGTIRMHQEREF
PERISATELKNPDFAALAESYGGWSARAKTTDEFKTGLVEAEGRSGLRLIHCLIDVEQLAASGATISGLRSR

Specific function: Catalyzes the first step in the biosynthesis of branched-chain amino acids [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=546, Percent_Identity=23.0769230769231, Blast_Score=113, Evalue=6e-25,
Organism=Homo sapiens, GI21361361, Length=506, Percent_Identity=21.5415019762846, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI87081685, Length=540, Percent_Identity=28.7037037037037, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI1790104, Length=552, Percent_Identity=27.3550724637681, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1786717, Length=571, Percent_Identity=25.3940455341506, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788716, Length=557, Percent_Identity=23.3393177737882, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1787096, Length=544, Percent_Identity=23.3455882352941, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17531301, Length=572, Percent_Identity=26.048951048951, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17531299, Length=572, Percent_Identity=26.048951048951, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17542570, Length=562, Percent_Identity=23.1316725978648, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323755, Length=552, Percent_Identity=27.1739130434783, Blast_Score=190, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6320816, Length=542, Percent_Identity=21.7712177121771, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19922626, Length=495, Percent_Identity=27.4747474747475, Blast_Score=138, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59519; Mature: 59388

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSTTPDAARLLVDCLVEQGCDRVFTVPGESFLQVLDGLHQHGSIDVVTCRQEGGVAFM
CCCCCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEE
ACADGAMTNRPGVAFVTRGPGATNASIGVHVALQDSQPMILFVGDVGRDMREREGFQEID
EECCCCCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCEEEEECCCCCHHHHHCCCHHHH
FLAFFGPIAKWAARIDSADRIPEFVARAYSVAMSGRPGPVVLALPEDMLSDTTTARPRPQ
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHCCCCCCCCCCC
VARPAQAVCPDAMQAMMAMIGDAASPVAIIGGAGWHSKAREHFQLFAERIGLPVATAFRR
CCCCHHHCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHH
QDAISPASPVYAGNLGYGPNPKLVERVRNADLILAVGARLGEATTEGYTLITPDHPDQML
HCCCCCCCCCEECCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEECCCCCCCEE
IHVHPDPDELGSVYRTDLSLCADMGEFAEAAALWEENSVISFDAGAEAHAEWEAWATATP
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHEECCCC
GDWQLDLARCVTFMRDTLPADTIICNGAGNFAGWWHRYWRYRGHPTQLAPTCGAMGYGVP
CCCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHH
AAVAAARRFPNRTVVAIAGDGDFLMNGQELATAAQYSLNLMVIIVDNGAYGTIRMHQERE
HHHHHHHHCCCCEEEEEECCCCEEECCHHHHHHHHCCEEEEEEEECCCCEEEEEECCCCC
FPERISATELKNPDFAALAESYGGWSARAKTTDEFKTGLVEAEGRSGLRLIHCLIDVEQL
CHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHHHHCCEECCCCCCHHHHHHHHHHHHH
AASGATISGLRSR
HCCCCCCCCCCCC
>Mature Secondary Structure 
TRSTTPDAARLLVDCLVEQGCDRVFTVPGESFLQVLDGLHQHGSIDVVTCRQEGGVAFM
CCCCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEE
ACADGAMTNRPGVAFVTRGPGATNASIGVHVALQDSQPMILFVGDVGRDMREREGFQEID
EECCCCCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCEEEEECCCCCHHHHHCCCHHHH
FLAFFGPIAKWAARIDSADRIPEFVARAYSVAMSGRPGPVVLALPEDMLSDTTTARPRPQ
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHCCCCCCCCCCC
VARPAQAVCPDAMQAMMAMIGDAASPVAIIGGAGWHSKAREHFQLFAERIGLPVATAFRR
CCCCHHHCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHH
QDAISPASPVYAGNLGYGPNPKLVERVRNADLILAVGARLGEATTEGYTLITPDHPDQML
HCCCCCCCCCEECCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEECCCCCCCEE
IHVHPDPDELGSVYRTDLSLCADMGEFAEAAALWEENSVISFDAGAEAHAEWEAWATATP
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHEECCCC
GDWQLDLARCVTFMRDTLPADTIICNGAGNFAGWWHRYWRYRGHPTQLAPTCGAMGYGVP
CCCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHH
AAVAAARRFPNRTVVAIAGDGDFLMNGQELATAAQYSLNLMVIIVDNGAYGTIRMHQERE
HHHHHHHHCCCCEEEEEECCCCEEECCHHHHHHHHCCEEEEEEEECCCCEEEEEECCCCC
FPERISATELKNPDFAALAESYGGWSARAKTTDEFKTGLVEAEGRSGLRLIHCLIDVEQL
CHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHHHHCCEECCCCCCHHHHHHHHHHHHH
AASGATISGLRSR
HCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3550695; 1379743; 9278503; 1995430; 6154938; 7015336; 3897211 [H]