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The map label for this gene is rlmB [H]

Identifier: 85373568

GI number: 85373568

Start: 766186

End: 766974

Strand: Reverse

Name: rlmB [H]

Synonym: ELI_03705

Alternate gene names: 85373568

Gene position: 766974-766186 (Counterclockwise)

Preceding gene: 85373569

Following gene: 85373567

Centisome position: 25.13

GC content: 68.06

Gene sequence:

>789_bases
ATGGCAAAGGGTGAACGCAAGAGAGCGATGCGCGGTCGCGCGGGCCGGATGCAGGGCGGGCGCGGCAGTGGCCGGGCGAC
GCCGGGGCATGTCCGGCTCTGGGGTCGGCACGCGGTCGAAGCCGCCTTGCTAAACCCCGCCCGCGTCCATCGCAAGCTGT
GGGCGACGCGCGAAGGGGTCGCTTCGCTCGACGGCGAATTGCCGCCCGACTTCCCGGTCGAATATGCCGATGTCACCGAC
CTCGCCCGCCTTGTCGCACGCGATGCGCCGCACCAGGGTCTGGTGCTCGAATGCGAGCAATTGAAGGATGTGTACATCGA
TGACGTGCTGGAAGAGGGGCGCCCGGTGGTGATCCTCGACCAGGTCACCGACCCGCACAATGTTGGCGCCATCCTACGCT
CCGCCGCCGCCTTCGATGCCGCCGCGATCGTGACGCAGGATCGCCATGCGCCGCCGGAATCCGGCGTTGTCGCCAAGTCC
GCATCCGGCGCGCTGGAGACGGTGCCGTGGGTCCGCGTCGTGAACCTCGCCCGCGCGCTCGATACGCTGGCGGAGCGCGG
CTACTGGCGGATCGGTCTTACAGGCAACACCGAGGCAACACTCGCGGAAGCCCTGCCGGCCGGGCCGGTCGCGCTGGTAC
TCGGCGCGGAAGGCGAAGGCATGCGGCACAATATCGAGGCGCATTGCGACGCCCTCGCCCGGCTGCCGATCAGTAACGCA
ATCGAAAGTCTCAACGTGTCCAATGCTGCCGCAATCGCGCTTTATGCCGTGGCCACACGCGCAACCTGA

Upstream 100 bases:

>100_bases
CCGTTTGCCGAGAGGCGCCCCTTTGGCGAAGCGGCGCTTGCCTGTCAATGGGGGGGCTGGCAGGGCGGCGGCGAGAATTG
GAAAAGCAGGACTTCACGGT

Downstream 100 bases:

>100_bases
ATTCATTTTTAAAGGGGGGAACATGCTGCGAGATATCAGAAACCTGGGACGATTTTTGGCGACCGGCGCGCTCGCTATAT
TGCTTACCGGCTGCTTCATG

Product: rRNA methylase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MAKGERKRAMRGRAGRMQGGRGSGRATPGHVRLWGRHAVEAALLNPARVHRKLWATREGVASLDGELPPDFPVEYADVTD
LARLVARDAPHQGLVLECEQLKDVYIDDVLEEGRPVVILDQVTDPHNVGAILRSAAAFDAAAIVTQDRHAPPESGVVAKS
ASGALETVPWVRVVNLARALDTLAERGYWRIGLTGNTEATLAEALPAGPVALVLGAEGEGMRHNIEAHCDALARLPISNA
IESLNVSNAAAIALYAVATRAT

Sequences:

>Translated_262_residues
MAKGERKRAMRGRAGRMQGGRGSGRATPGHVRLWGRHAVEAALLNPARVHRKLWATREGVASLDGELPPDFPVEYADVTD
LARLVARDAPHQGLVLECEQLKDVYIDDVLEEGRPVVILDQVTDPHNVGAILRSAAAFDAAAIVTQDRHAPPESGVVAKS
ASGALETVPWVRVVNLARALDTLAERGYWRIGLTGNTEATLAEALPAGPVALVLGAEGEGMRHNIEAHCDALARLPISNA
IESLNVSNAAAIALYAVATRAT
>Mature_261_residues
AKGERKRAMRGRAGRMQGGRGSGRATPGHVRLWGRHAVEAALLNPARVHRKLWATREGVASLDGELPPDFPVEYADVTDL
ARLVARDAPHQGLVLECEQLKDVYIDDVLEEGRPVVILDQVTDPHNVGAILRSAAAFDAAAIVTQDRHAPPESGVVAKSA
SGALETVPWVRVVNLARALDTLAERGYWRIGLTGNTEATLAEALPAGPVALVLGAEGEGMRHNIEAHCDALARLPISNAI
ESLNVSNAAAIALYAVATRAT

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=159, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1790623, Length=173, Percent_Identity=43.3526011560694, Blast_Score=120, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6324775, Length=301, Percent_Identity=26.578073089701, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27836; Mature: 27705

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGERKRAMRGRAGRMQGGRGSGRATPGHVRLWGRHAVEAALLNPARVHRKLWATREGV
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHCCHHHHHHHHHHHHHHH
ASLDGELPPDFPVEYADVTDLARLVARDAPHQGLVLECEQLKDVYIDDVLEEGRPVVILD
HHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEE
QVTDPHNVGAILRSAAAFDAAAIVTQDRHAPPESGVVAKSASGALETVPWVRVVNLARAL
CCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHH
DTLAERGYWRIGLTGNTEATLAEALPAGPVALVLGAEGEGMRHNIEAHCDALARLPISNA
HHHHHCCEEEEEECCCCHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCHHHH
IESLNVSNAAAIALYAVATRAT
HHHCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKGERKRAMRGRAGRMQGGRGSGRATPGHVRLWGRHAVEAALLNPARVHRKLWATREGV
CCCHHHHHHHCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHCCHHHHHHHHHHHHHHH
ASLDGELPPDFPVEYADVTDLARLVARDAPHQGLVLECEQLKDVYIDDVLEEGRPVVILD
HHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEE
QVTDPHNVGAILRSAAAFDAAAIVTQDRHAPPESGVVAKSASGALETVPWVRVVNLARAL
CCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHH
DTLAERGYWRIGLTGNTEATLAEALPAGPVALVLGAEGEGMRHNIEAHCDALARLPISNA
HHHHHCCEEEEEECCCCHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCHHHH
IESLNVSNAAAIALYAVATRAT
HHHCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]