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The map label for this gene is gdhA [H]

Identifier: 85373541

GI number: 85373541

Start: 737719

End: 739074

Strand: Reverse

Name: gdhA [H]

Synonym: ELI_03570

Alternate gene names: 85373541

Gene position: 739074-737719 (Counterclockwise)

Preceding gene: 85373542

Following gene: 85373540

Centisome position: 24.21

GC content: 61.06

Gene sequence:

>1356_bases
ATGGCCGCAACAGACCACGTCGATTTCGAAAAATTCATGGAGGGCGTAAACCGCCGCAACCCGCACCAGCCAGAATTCGT
GCAGGCCGTGCAGGAAGTGGCACAGGACATCTTCGAATTCATCGAGGACAAGGAGCGATACCACTCCGAACAGATCCTCC
GCCGCCTGACCGAGCCTGATCGCATCGTCAGTTTCCGGGTCTGCTGGGAAGACGACAAAGGCGATATTCGCGTGCAGCGC
GGCTGGCGGGTGCAGAACAACAACGCCATCGGGCCTTACAAGGGTGGGCTGCGGTTCCACCCTTCCGTCACCGAGAGCGT
GCTCAAGTTCCTCGCTTTCGAACAGACCTTCAAGAACGCGCTCACGGGCCTGCCGATGGGCGGCGGCAAGGGCGGATCGA
ACTTCAACCCCAAGGGCAAGAGCGATCGCGAAATCATGCGCTTCTGCCAGAGCTTCATGACAGAACTCTATCGCCATATC
GGCCCCGACACCGACGTGCCGGCCGGCGATATCGGCGTCGGATCGCGCGAAATCGGTTACATGTTCGGGCAGTACAAGCG
GATCACCAATCATTTCACCGGCGTCCTGACCGGCAAGGGGCTGGAATTCGGCGGCTCGCCCTTCCGCCCGGAATCGACCG
GCTACGGCGCAGTGCTGTTCCTGTGCCACATGTTGAGCCACCGGGACGACGCGATCGATGGCAAGACGCTGGTCATCTCC
GGCGCCGGCAACGTGGCCACCCATGCGGCGGAGAAGGCCGTGCAACTGGGCGGCAAGGTCGTGACGCTGTCGGATTCCGA
TGGCTTCGTTTACGATCCGGACGGCTTCGATCAGGAAAAGATCGACTGGGTTCGCCGGCTCAAGGAAGAAAATGACGGCA
GCCTTTCGGCCTATCCCGAGGAATTTTCCAATGCCGAGTTCCACGAAGGCAAGCGGCCGTGGCAGGTCGAATGCGATATC
GCGATGCCCTGCGCGACGCAGAACGAATTGTGCAAGGACGATGCGAAAACCCTGATCGACAACGGCTGCATGGCGGTGGT
CGAAGGCGCCAATATGCCGACCGAGCTCGAAGCCGCCCATGTCATCAAGGACGCCAAAATCCTGTTCGGCCCGGGCAAGG
CCGCCAATGCCGGCGGCGTCGCCGTATCGGGCCTGGAAATGAGCCAGAACTCCGCCCGCCGGACGTGGGAACAGGTCGAA
CTCGAACAGATGCTGCAGGACATCATGAAAGGCATCCACGAACGCTGCCTGAAGCACGGCGAGCAGGACGACGGCTACGT
CGATTACGTCAAGGGCGCCAATATCGCCGGTTTCAAGAAAGTGGCCGACGCGATGCTCGCCTTCGGCGTGGTGTGA

Upstream 100 bases:

>100_bases
GTTGTCTTCGCCACTCGGCCCCCTTTCGCCGACCTGTGCGTTGCCCACGCATTAGCCACCCAATTCGCAAACCTGCCGCG
TCGCCGGAAGAAGGAACCTC

Downstream 100 bases:

>100_bases
CGCTGCGCGCAAACAGCCGGATGTGGTAGAGTAGGGTAAATGGCGGAGAGGGAGGGATTCGAACCCTCGATACGGTTGCC
CGTATACTGCATTTCGAGTG

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: GDH; NAD(P)H-utilizing glutamate dehydrogenase [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MAATDHVDFEKFMEGVNRRNPHQPEFVQAVQEVAQDIFEFIEDKERYHSEQILRRLTEPDRIVSFRVCWEDDKGDIRVQR
GWRVQNNNAIGPYKGGLRFHPSVTESVLKFLAFEQTFKNALTGLPMGGGKGGSNFNPKGKSDREIMRFCQSFMTELYRHI
GPDTDVPAGDIGVGSREIGYMFGQYKRITNHFTGVLTGKGLEFGGSPFRPESTGYGAVLFLCHMLSHRDDAIDGKTLVIS
GAGNVATHAAEKAVQLGGKVVTLSDSDGFVYDPDGFDQEKIDWVRRLKEENDGSLSAYPEEFSNAEFHEGKRPWQVECDI
AMPCATQNELCKDDAKTLIDNGCMAVVEGANMPTELEAAHVIKDAKILFGPGKAANAGGVAVSGLEMSQNSARRTWEQVE
LEQMLQDIMKGIHERCLKHGEQDDGYVDYVKGANIAGFKKVADAMLAFGVV

Sequences:

>Translated_451_residues
MAATDHVDFEKFMEGVNRRNPHQPEFVQAVQEVAQDIFEFIEDKERYHSEQILRRLTEPDRIVSFRVCWEDDKGDIRVQR
GWRVQNNNAIGPYKGGLRFHPSVTESVLKFLAFEQTFKNALTGLPMGGGKGGSNFNPKGKSDREIMRFCQSFMTELYRHI
GPDTDVPAGDIGVGSREIGYMFGQYKRITNHFTGVLTGKGLEFGGSPFRPESTGYGAVLFLCHMLSHRDDAIDGKTLVIS
GAGNVATHAAEKAVQLGGKVVTLSDSDGFVYDPDGFDQEKIDWVRRLKEENDGSLSAYPEEFSNAEFHEGKRPWQVECDI
AMPCATQNELCKDDAKTLIDNGCMAVVEGANMPTELEAAHVIKDAKILFGPGKAANAGGVAVSGLEMSQNSARRTWEQVE
LEQMLQDIMKGIHERCLKHGEQDDGYVDYVKGANIAGFKKVADAMLAFGVV
>Mature_450_residues
AATDHVDFEKFMEGVNRRNPHQPEFVQAVQEVAQDIFEFIEDKERYHSEQILRRLTEPDRIVSFRVCWEDDKGDIRVQRG
WRVQNNNAIGPYKGGLRFHPSVTESVLKFLAFEQTFKNALTGLPMGGGKGGSNFNPKGKSDREIMRFCQSFMTELYRHIG
PDTDVPAGDIGVGSREIGYMFGQYKRITNHFTGVLTGKGLEFGGSPFRPESTGYGAVLFLCHMLSHRDDAIDGKTLVISG
AGNVATHAAEKAVQLGGKVVTLSDSDGFVYDPDGFDQEKIDWVRRLKEENDGSLSAYPEEFSNAEFHEGKRPWQVECDIA
MPCATQNELCKDDAKTLIDNGCMAVVEGANMPTELEAAHVIKDAKILFGPGKAANAGGVAVSGLEMSQNSARRTWEQVEL
EQMLQDIMKGIHERCLKHGEQDDGYVDYVKGANIAGFKKVADAMLAFGVV

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=400, Percent_Identity=28.75, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI4885281, Length=400, Percent_Identity=28.25, Blast_Score=162, Evalue=5e-40,
Organism=Escherichia coli, GI1788059, Length=444, Percent_Identity=55.6306306306306, Blast_Score=512, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI17544676, Length=355, Percent_Identity=29.2957746478873, Blast_Score=142, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6324951, Length=447, Percent_Identity=48.3221476510067, Blast_Score=382, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6319256, Length=454, Percent_Identity=48.4581497797357, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI45549226, Length=366, Percent_Identity=29.5081967213115, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI45553475, Length=366, Percent_Identity=29.5081967213115, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24649283, Length=360, Percent_Identity=31.3888888888889, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24649460, Length=289, Percent_Identity=30.7958477508651, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 49975; Mature: 49844

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATDHVDFEKFMEGVNRRNPHQPEFVQAVQEVAQDIFEFIEDKERYHSEQILRRLTEPD
CCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RIVSFRVCWEDDKGDIRVQRGWRVQNNNAIGPYKGGLRFHPSVTESVLKFLAFEQTFKNA
CEEEEEEEEECCCCCEEEEECEEECCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHH
LTGLPMGGGKGGSNFNPKGKSDREIMRFCQSFMTELYRHIGPDTDVPAGDIGVGSREIGY
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
MFGQYKRITNHFTGVLTGKGLEFGGSPFRPESTGYGAVLFLCHMLSHRDDAIDGKTLVIS
HHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEE
GAGNVATHAAEKAVQLGGKVVTLSDSDGFVYDPDGFDQEKIDWVRRLKEENDGSLSAYPE
CCCCHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCH
EFSNAEFHEGKRPWQVECDIAMPCATQNELCKDDAKTLIDNGCMAVVEGANMPTELEAAH
HHCCCCHHCCCCCEEEEECEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHH
VIKDAKILFGPGKAANAGGVAVSGLEMSQNSARRTWEQVELEQMLQDIMKGIHERCLKHG
HHHCCEEEECCCCCCCCCCEEEECCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EQDDGYVDYVKGANIAGFKKVADAMLAFGVV
CCCCCCEEEECCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AATDHVDFEKFMEGVNRRNPHQPEFVQAVQEVAQDIFEFIEDKERYHSEQILRRLTEPD
CCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RIVSFRVCWEDDKGDIRVQRGWRVQNNNAIGPYKGGLRFHPSVTESVLKFLAFEQTFKNA
CEEEEEEEEECCCCCEEEEECEEECCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHH
LTGLPMGGGKGGSNFNPKGKSDREIMRFCQSFMTELYRHIGPDTDVPAGDIGVGSREIGY
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
MFGQYKRITNHFTGVLTGKGLEFGGSPFRPESTGYGAVLFLCHMLSHRDDAIDGKTLVIS
HHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEE
GAGNVATHAAEKAVQLGGKVVTLSDSDGFVYDPDGFDQEKIDWVRRLKEENDGSLSAYPE
CCCCHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCH
EFSNAEFHEGKRPWQVECDIAMPCATQNELCKDDAKTLIDNGCMAVVEGANMPTELEAAH
HHCCCCHHCCCCCEEEEECEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHH
VIKDAKILFGPGKAANAGGVAVSGLEMSQNSARRTWEQVELEQMLQDIMKGIHERCLKHG
HHHCCEEEECCCCCCCCCCEEEECCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EQDDGYVDYVKGANIAGFKKVADAMLAFGVV
CCCCCCEEEECCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955404; 12663928 [H]