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The map label for this gene is truB [H]

Identifier: 85373532

GI number: 85373532

Start: 729012

End: 730100

Strand: Direct

Name: truB [H]

Synonym: ELI_03525

Alternate gene names: 85373532

Gene position: 729012-730100 (Clockwise)

Preceding gene: 85373531

Following gene: 85373533

Centisome position: 23.88

GC content: 64.46

Gene sequence:

>1089_bases
ATGAGCGACCTCAAACCCGCCCCCCACGGCTGGATCATCCTCGACAAACCGCGCGGGCTCGGCTCGACGCAGGCGGTGGG
GGCGGTGAAGCGAAACTTGCGCGAGGGCGGTTACGCCAAGACCAAGGTGGGGCACGGCGGGACGCTCGATCCCGAAGCCG
AAGGGGTGCTGCCGATTGCTTTGGGGGAGGCCACCAAGCTCGCCGGGCGGATGCTCGATGCGTCGAAGATCTACGAATTC
ACCGTCCAGTTCGGCGAGGAGACCGACACGCTCGATGCCGAGGGCGAAATCACCGCTCGCTCGGATCGCCGACCGCCGAT
GGCGGCAATCGCAGCGGTGCTGGAGCATTTCACCGGCGAAATCGAGCAAGTCCCGCCCAAATATTCCGCGCTGATGGTCG
ATGGCAAGCGCGCCTACGACCGGGCGCGGGCTGGCGAAGAGGTGGAACTGAAGACGCGGCGTGTAACCATCTACGATATT
TCTTTATCCCGTCATCCCCGCGAAGGCGGGGATCCAGCTAACCTTGGTGACCACGACGAAAGTCAGCTGGACCCCCGCCT
TCGCGGGGGTGACGGCGTGGGGTTTTTGGCGGATGCGGACTTAACTTCCACCTTCCAGACCACCAGCGGCCGCCCCGATC
CCTACGACCCCGGCGCACCGCTGGAACTGGCCGATGCCATCACGCTGACGGCACATGTCTCGAAGGGCACCTATATCCGC
AGTCTGGCACGGGATATCGCGCGGGCCCTTGGAACCGTCGGCCACGTTACCTATCTACGTCGCATCAAGGCGGGGCCCTT
CGCTGAATTCCAGGCGATTTCGCTGGACAAACTCAACGAGATCGGTAAGGGCGCGCCACTTCAAGACCTCCTCCTGCCGC
TCGAGGCGGGGCTGGACGACATCCCGGCTCTGACACTCGATTCTGACAGCGCGCAGGCGGTCCGCCAGGGCCGGGTCCTG
TCAGAGCTGCCCCAGACCGACGGGCTTTACCTTGCGAAGCTGGACGATGTGCCGGTCGCGCTGGTGGAACTCAGCGGTGG
TACGGCCAGGGTCGTGCGGGGTTTCAACCTTCCCGATGTCGCTGAGTAA

Upstream 100 bases:

>100_bases
TGTTCAGCTCTGTCGCCGTGGCCTTGCTGACCAACTTCATCGTCTTTGGAAACCGCGCGCGGCTTGTATTCTTTGCCGGC
GATCCCCAGCCTGAGAAGGC

Downstream 100 bases:

>100_bases
GAGAGAACATACATGTCGGTAGACGCCGAAACGAAAACCAAGATCATCAAGGACAACGCCCGCGACAAGAACGACACGGG
CAGCCCGGAAGTCCAGGTCG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MSDLKPAPHGWIILDKPRGLGSTQAVGAVKRNLREGGYAKTKVGHGGTLDPEAEGVLPIALGEATKLAGRMLDASKIYEF
TVQFGEETDTLDAEGEITARSDRRPPMAAIAAVLEHFTGEIEQVPPKYSALMVDGKRAYDRARAGEEVELKTRRVTIYDI
SLSRHPREGGDPANLGDHDESQLDPRLRGGDGVGFLADADLTSTFQTTSGRPDPYDPGAPLELADAITLTAHVSKGTYIR
SLARDIARALGTVGHVTYLRRIKAGPFAEFQAISLDKLNEIGKGAPLQDLLLPLEAGLDDIPALTLDSDSAQAVRQGRVL
SELPQTDGLYLAKLDDVPVALVELSGGTARVVRGFNLPDVAE

Sequences:

>Translated_362_residues
MSDLKPAPHGWIILDKPRGLGSTQAVGAVKRNLREGGYAKTKVGHGGTLDPEAEGVLPIALGEATKLAGRMLDASKIYEF
TVQFGEETDTLDAEGEITARSDRRPPMAAIAAVLEHFTGEIEQVPPKYSALMVDGKRAYDRARAGEEVELKTRRVTIYDI
SLSRHPREGGDPANLGDHDESQLDPRLRGGDGVGFLADADLTSTFQTTSGRPDPYDPGAPLELADAITLTAHVSKGTYIR
SLARDIARALGTVGHVTYLRRIKAGPFAEFQAISLDKLNEIGKGAPLQDLLLPLEAGLDDIPALTLDSDSAQAVRQGRVL
SELPQTDGLYLAKLDDVPVALVELSGGTARVVRGFNLPDVAE
>Mature_361_residues
SDLKPAPHGWIILDKPRGLGSTQAVGAVKRNLREGGYAKTKVGHGGTLDPEAEGVLPIALGEATKLAGRMLDASKIYEFT
VQFGEETDTLDAEGEITARSDRRPPMAAIAAVLEHFTGEIEQVPPKYSALMVDGKRAYDRARAGEEVELKTRRVTIYDIS
LSRHPREGGDPANLGDHDESQLDPRLRGGDGVGFLADADLTSTFQTTSGRPDPYDPGAPLELADAITLTAHVSKGTYIRS
LARDIARALGTVGHVTYLRRIKAGPFAEFQAISLDKLNEIGKGAPLQDLLLPLEAGLDDIPALTLDSDSAQAVRQGRVLS
ELPQTDGLYLAKLDDVPVALVELSGGTARVVRGFNLPDVAE

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=171, Percent_Identity=39.766081871345, Blast_Score=98, Evalue=9e-21,
Organism=Escherichia coli, GI2367200, Length=318, Percent_Identity=29.874213836478, Blast_Score=129, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6324037, Length=285, Percent_Identity=32.6315789473684, Blast_Score=100, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 38702; Mature: 38571

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLKPAPHGWIILDKPRGLGSTQAVGAVKRNLREGGYAKTKVGHGGTLDPEAEGVLPIA
CCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCEEEEE
LGEATKLAGRMLDASKIYEFTVQFGEETDTLDAEGEITARSDRRPPMAAIAAVLEHFTGE
CCCHHHHHHHHHCHHHHEEEEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCH
IEQVPPKYSALMVDGKRAYDRARAGEEVELKTRRVTIYDISLSRHPREGGDPANLGDHDE
HHHCCCCCEEEEECCHHHHHHHHCCCCEEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCH
SQLDPRLRGGDGVGFLADADLTSTFQTTSGRPDPYDPGAPLELADAITLTAHVSKGTYIR
HHCCCCCCCCCCEEEEECCCCCHHHHCCCCCCCCCCCCCCCHHHCEEEEEEECCCCHHHH
SLARDIARALGTVGHVTYLRRIKAGPFAEFQAISLDKLNEIGKGAPLQDLLLPLEAGLDD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHCCCCCHHHHHHHHHCCCCC
IPALTLDSDSAQAVRQGRVLSELPQTDGLYLAKLDDVPVALVELSGGTARVVRGFNLPDV
CCEEEECCCHHHHHHHCCHHHHCCCCCCEEEEEECCCCEEEEEECCCCEEEEECCCCCCC
AE
CC
>Mature Secondary Structure 
SDLKPAPHGWIILDKPRGLGSTQAVGAVKRNLREGGYAKTKVGHGGTLDPEAEGVLPIA
CCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCEEEEE
LGEATKLAGRMLDASKIYEFTVQFGEETDTLDAEGEITARSDRRPPMAAIAAVLEHFTGE
CCCHHHHHHHHHCHHHHEEEEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCH
IEQVPPKYSALMVDGKRAYDRARAGEEVELKTRRVTIYDISLSRHPREGGDPANLGDHDE
HHHCCCCCEEEEECCHHHHHHHHCCCCEEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCH
SQLDPRLRGGDGVGFLADADLTSTFQTTSGRPDPYDPGAPLELADAITLTAHVSKGTYIR
HHCCCCCCCCCCEEEEECCCCCHHHHCCCCCCCCCCCCCCCHHHCEEEEEEECCCCHHHH
SLARDIARALGTVGHVTYLRRIKAGPFAEFQAISLDKLNEIGKGAPLQDLLLPLEAGLDD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHCCCCCHHHHHHHHHCCCCC
IPALTLDSDSAQAVRQGRVLSELPQTDGLYLAKLDDVPVALVELSGGTARVVRGFNLPDV
CCEEEECCCHHHHHHHCCHHHHCCCCCCEEEEEECCCCEEEEEECCCCEEEEECCCCCCC
AE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA