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The map label for this gene is pspF [H]

Identifier: 85373508

GI number: 85373508

Start: 706282

End: 707322

Strand: Direct

Name: pspF [H]

Synonym: ELI_03405

Alternate gene names: 85373508

Gene position: 706282-707322 (Clockwise)

Preceding gene: 85373492

Following gene: 85373511

Centisome position: 23.14

GC content: 64.17

Gene sequence:

>1041_bases
ATGGAGCGCGGGAATCAGTTTGTCGGCCAGTCCACGGCTTTTCTCGACGCGGTCGAACGCGCAAGCCGCGCAGCCCCGAT
GCAGCGCCCGATCCTTATTATCGGGGAGCGCGGCACAGGCAAGGAACTGATTGCCGAACGTCTGCATCGACTCTCACCAC
GATGGGGCGAGCCGCTGGTCATCATGAACTGCGCGGCGCTCCCCGAAACCCTTATCGAGGCCGAACTGTTCGGCCACGAA
GCGGGCGCTTTTACCGGCGCTACCAGAGCGCGCGAGGGAAGGTTCGAGGAAGCCGACAAGGGCACTCTATTCCTCGACGA
ACTGGGTACTTTGTCGATGGCCGCACAGGAACGGCTGTTGAGGGCCGTCGAATATGGCGAGGTCACGCGGATCGGCTCGT
CGCGCCCGGTCAATGTGGATGTGCGGATCGTCGCCGCCACCAACGAGGACCTGCCCCGACTCGCGGAGGAAGGCACCTTC
CGCGCCGACTTGCTCGACCGGCTCAGTTTCGAAGTCATTACCTTGCCGCCCCTGCGCGTGCGCGATGGCGATGTCGGCGT
GCTGACGGAATATTTCGGTCGGCGCATGGCGGCCGAGCTCGCCTGGGAAGGCTGGCCGGGTTTTGCGCCGCATGTGCAGA
AAGAGCTGGAGGATTATGCCTGGCCCGGCAACGTGCGCGAGCTGCGCAATGTTGTCGAGCGAGCGGTCTATCGCTGGGAC
AGCTGGGAGGAGCCGATCGGCTATGTCCAGTTCGATCCCTTCGAGAGCCCGTGGAAACCCTTGCCCGGCGAAGCTGCCAA
GCGCGACAAGTCGACCGACTTTCGCGACGCCGTCGACCAGACGGCGCGCCCGACGCTGGATCTCGATTGCGACGATCTGC
GCGGCGCGGTGGACGAGCATGAGCGGGCGATCATCGAACACGCGCTCGGCAAGCACCGCTGGAACCAGCGCCAGACCGCC
AAGGCGCTCGGCTTGACCTACGACCAGCTGCGCCATTGCATCAAGAAGCATGGACTGATGGAAGAGGCGACCAATTCCTA
G

Upstream 100 bases:

>100_bases
AATGGCAGAAACAGGAGAATTCAGCGCTTCTTAATGTTTTTCTCCGGACGGGTGTTGGCGAGTATTGCCAACCCTTGGGA
AAAATTCCTATATATCGGGT

Downstream 100 bases:

>100_bases
TTCGCGAAGCCCAGAACGGCCATGAGGCCCCGCTGAACCTCAAGCATGGTTTCCCGGTCGAGCTCGCCGATCTTGCTTTG
CAACCGCGATCGGCGCAGCG

Product: transcriptional regulator

Products: NA

Alternate protein names: Phage shock protein F [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MERGNQFVGQSTAFLDAVERASRAAPMQRPILIIGERGTGKELIAERLHRLSPRWGEPLVIMNCAALPETLIEAELFGHE
AGAFTGATRAREGRFEEADKGTLFLDELGTLSMAAQERLLRAVEYGEVTRIGSSRPVNVDVRIVAATNEDLPRLAEEGTF
RADLLDRLSFEVITLPPLRVRDGDVGVLTEYFGRRMAAELAWEGWPGFAPHVQKELEDYAWPGNVRELRNVVERAVYRWD
SWEEPIGYVQFDPFESPWKPLPGEAAKRDKSTDFRDAVDQTARPTLDLDCDDLRGAVDEHERAIIEHALGKHRWNQRQTA
KALGLTYDQLRHCIKKHGLMEEATNS

Sequences:

>Translated_346_residues
MERGNQFVGQSTAFLDAVERASRAAPMQRPILIIGERGTGKELIAERLHRLSPRWGEPLVIMNCAALPETLIEAELFGHE
AGAFTGATRAREGRFEEADKGTLFLDELGTLSMAAQERLLRAVEYGEVTRIGSSRPVNVDVRIVAATNEDLPRLAEEGTF
RADLLDRLSFEVITLPPLRVRDGDVGVLTEYFGRRMAAELAWEGWPGFAPHVQKELEDYAWPGNVRELRNVVERAVYRWD
SWEEPIGYVQFDPFESPWKPLPGEAAKRDKSTDFRDAVDQTARPTLDLDCDDLRGAVDEHERAIIEHALGKHRWNQRQTA
KALGLTYDQLRHCIKKHGLMEEATNS
>Mature_346_residues
MERGNQFVGQSTAFLDAVERASRAAPMQRPILIIGERGTGKELIAERLHRLSPRWGEPLVIMNCAALPETLIEAELFGHE
AGAFTGATRAREGRFEEADKGTLFLDELGTLSMAAQERLLRAVEYGEVTRIGSSRPVNVDVRIVAATNEDLPRLAEEGTF
RADLLDRLSFEVITLPPLRVRDGDVGVLTEYFGRRMAAELAWEGWPGFAPHVQKELEDYAWPGNVRELRNVVERAVYRWD
SWEEPIGYVQFDPFESPWKPLPGEAAKRDKSTDFRDAVDQTARPTLDLDCDDLRGAVDEHERAIIEHALGKHRWNQRQTA
KALGLTYDQLRHCIKKHGLMEEATNS

Specific function: Transcriptional activator for the phage shock protein (psp) operon (pspABCDE) and pspG gene [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87081872, Length=338, Percent_Identity=49.112426035503, Blast_Score=336, Evalue=1e-93,
Organism=Escherichia coli, GI1789233, Length=341, Percent_Identity=38.7096774193548, Blast_Score=222, Evalue=2e-59,
Organism=Escherichia coli, GI1790437, Length=329, Percent_Identity=41.6413373860182, Blast_Score=214, Evalue=9e-57,
Organism=Escherichia coli, GI87082117, Length=232, Percent_Identity=47.8448275862069, Blast_Score=211, Evalue=4e-56,
Organism=Escherichia coli, GI1788550, Length=334, Percent_Identity=37.4251497005988, Blast_Score=207, Evalue=8e-55,
Organism=Escherichia coli, GI87082152, Length=328, Percent_Identity=40.8536585365854, Blast_Score=207, Evalue=9e-55,
Organism=Escherichia coli, GI1790299, Length=333, Percent_Identity=38.4384384384384, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1789087, Length=231, Percent_Identity=45.021645021645, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1788905, Length=230, Percent_Identity=42.6086956521739, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1787583, Length=342, Percent_Identity=34.2105263157895, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1786524, Length=216, Percent_Identity=47.2222222222222, Blast_Score=171, Evalue=9e-44,
Organism=Escherichia coli, GI1789828, Length=234, Percent_Identity=36.7521367521368, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI87081858, Length=219, Percent_Identity=31.9634703196347, Blast_Score=108, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR014317 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 39055; Mature: 39055

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERGNQFVGQSTAFLDAVERASRAAPMQRPILIIGERGTGKELIAERLHRLSPRWGEPLV
CCCCCCHHCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCEE
IMNCAALPETLIEAELFGHEAGAFTGATRAREGRFEEADKGTLFLDELGTLSMAAQERLL
EEECHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHH
RAVEYGEVTRIGSSRPVNVDVRIVAATNEDLPRLAEEGTFRADLLDRLSFEVITLPPLRV
HHHHHCCCEECCCCCCCEEEEEEEEECCCCHHHHHHCCCHHHHHHHHCCCEEEECCCCEE
RDGDVGVLTEYFGRRMAAELAWEGWPGFAPHVQKELEDYAWPGNVRELRNVVERAVYRWD
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
SWEEPIGYVQFDPFESPWKPLPGEAAKRDKSTDFRDAVDQTARPTLDLDCDDLRGAVDEH
CCCCCCCCEEECCCCCCCCCCCCHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHH
ERAIIEHALGKHRWNQRQTAKALGLTYDQLRHCIKKHGLMEEATNS
HHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCC
>Mature Secondary Structure
MERGNQFVGQSTAFLDAVERASRAAPMQRPILIIGERGTGKELIAERLHRLSPRWGEPLV
CCCCCCHHCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCEE
IMNCAALPETLIEAELFGHEAGAFTGATRAREGRFEEADKGTLFLDELGTLSMAAQERLL
EEECHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHH
RAVEYGEVTRIGSSRPVNVDVRIVAATNEDLPRLAEEGTFRADLLDRLSFEVITLPPLRV
HHHHHCCCEECCCCCCCEEEEEEEEECCCCHHHHHHCCCHHHHHHHHCCCEEEECCCCEE
RDGDVGVLTEYFGRRMAAELAWEGWPGFAPHVQKELEDYAWPGNVRELRNVVERAVYRWD
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
SWEEPIGYVQFDPFESPWKPLPGEAAKRDKSTDFRDAVDQTARPTLDLDCDDLRGAVDEH
CCCCCCCCEEECCCCCCCCCCCCHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHH
ERAIIEHALGKHRWNQRQTAKALGLTYDQLRHCIKKHGLMEEATNS
HHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606168; 9097039; 9278503; 1712397; 7984428; 7920643 [H]