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The map label for this gene is pspA [H]

Identifier: 85373506

GI number: 85373506

Start: 705025

End: 705870

Strand: Reverse

Name: pspA [H]

Synonym: ELI_03395

Alternate gene names: 85373506

Gene position: 705870-705025 (Counterclockwise)

Preceding gene: 85373507

Following gene: 85373505

Centisome position: 23.13

GC content: 62.06

Gene sequence:

>846_bases
TTGTCCCGGCTCGAAGCCGAAGTAGAAAAGCTTCGCCGGAGCCCCACCCCGACGCAAGGTAAACAGAGCGAACCTTTTAC
CGCTTCGGAATGGGCCGACGCGGACAAGGTCAACGCCAAGGTCAACGAATTTCTCTCAGGAGCACCCTTCATGGGCATTT
TCAGCCGTACCCGCGATATCATTGCCGCCAATTTCAACGACATGCTCGACCAGGCCGACGACCCGGAAAAGATGATCCGC
ATGATCATCCTCGAGATGGAAGAAACGCTGGTCGAAGTGCGCGCCTCGGCCGCCCGCACCATCGCCGACCAGAAGGAAAT
GCATCGCCATACGGTCAAGCTCGACAAGCTGCAGGCCGACTGGGCCGAGAAGGCGCAGCTCGCGCTGTCGAAGGATCGCG
AAGATCTCGCCCGCGCCGCCCTCGTCGAGAAGAAAAAGGCCGCCGATATGAGCGACCAGCTCAAGCAGGAAATCGCCGTG
CTTGACGATGCGCTGCGCGCTTACGAGCAGGACATCCAGAAGCTGCAGGGCCGCCTGCGCGAAGCCCGCAGCCGCCAGAC
GGCGATCGCTGCCCGCCTCGAAAGCGCGGAAAATCGCGTCAAGCTGCGCAGCCTGATGACCAACGAGCGCGTCGACGATG
CGCTGACCCGCTTCGACCAGCTCGAACGCCGCGTCGACTATGCCGAAGGACGCGCCGAAGCCCTGAAGATTTCCGACCAG
TCGGGCACCCCCAGCCTCGCCGACGAAATCGATGCCCTCGCCGGCAGCGACAAGGTCGACGAGGAGCTCGAAGAAATGAA
ACGCGCCCTCGGAATGGGCGGCAAAGACGACAAGCAGGAGGACTAA

Upstream 100 bases:

>100_bases
CCGCGTGAGCAAGCCGAACAACCCCCTCGGACCGGAGCCCGCCTCCCCCCAGTCCCCTCGCTCTGACAAGAGCGCCGGCG
GGTCTCGGGTCCGTGACGGT

Downstream 100 bases:

>100_bases
GACCCCATGGAAGAAGTCATCATCATCCCGGCCATATTCATCGGGCTTCCCTGGTTGATCCTGCACTATGTCACGAAGTG
GAAGACCGCGGCCACCATCA

Product: sigma54-dependent transcription suppressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MSRLEAEVEKLRRSPTPTQGKQSEPFTASEWADADKVNAKVNEFLSGAPFMGIFSRTRDIIAANFNDMLDQADDPEKMIR
MIILEMEETLVEVRASAARTIADQKEMHRHTVKLDKLQADWAEKAQLALSKDREDLARAALVEKKKAADMSDQLKQEIAV
LDDALRAYEQDIQKLQGRLREARSRQTAIAARLESAENRVKLRSLMTNERVDDALTRFDQLERRVDYAEGRAEALKISDQ
SGTPSLADEIDALAGSDKVDEELEEMKRALGMGGKDDKQED

Sequences:

>Translated_281_residues
MSRLEAEVEKLRRSPTPTQGKQSEPFTASEWADADKVNAKVNEFLSGAPFMGIFSRTRDIIAANFNDMLDQADDPEKMIR
MIILEMEETLVEVRASAARTIADQKEMHRHTVKLDKLQADWAEKAQLALSKDREDLARAALVEKKKAADMSDQLKQEIAV
LDDALRAYEQDIQKLQGRLREARSRQTAIAARLESAENRVKLRSLMTNERVDDALTRFDQLERRVDYAEGRAEALKISDQ
SGTPSLADEIDALAGSDKVDEELEEMKRALGMGGKDDKQED
>Mature_280_residues
SRLEAEVEKLRRSPTPTQGKQSEPFTASEWADADKVNAKVNEFLSGAPFMGIFSRTRDIIAANFNDMLDQADDPEKMIRM
IILEMEETLVEVRASAARTIADQKEMHRHTVKLDKLQADWAEKAQLALSKDREDLARAALVEKKKAADMSDQLKQEIAVL
DDALRAYEQDIQKLQGRLREARSRQTAIAARLESAENRVKLRSLMTNERVDDALTRFDQLERRVDYAEGRAEALKISDQS
GTPSLADEIDALAGSDKVDEELEEMKRALGMGGKDDKQED

Specific function: The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspA negatively regulates expression of the pspABCDE promoter and of pspG through negative regulation

COG id: COG1842

COG function: function code KT; Phage shock protein A (IM30), suppresses sigma54-dependent transcription

Gene ontology:

Cell location: Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PspA/IM30 family [H]

Homologues:

Organism=Escherichia coli, GI2367118, Length=217, Percent_Identity=41.0138248847926, Blast_Score=166, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014319
- InterPro:   IPR007157 [H]

Pfam domain/function: PF04012 PspA_IM30 [H]

EC number: NA

Molecular weight: Translated: 31694; Mature: 31563

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLEAEVEKLRRSPTPTQGKQSEPFTASEWADADKVNAKVNEFLSGAPFMGIFSRTRDI
CCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
IAANFNDMLDQADDPEKMIRMIILEMEETLVEVRASAARTIADQKEMHRHTVKLDKLQAD
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WAEKAQLALSKDREDLARAALVEKKKAADMSDQLKQEIAVLDDALRAYEQDIQKLQGRLR
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EARSRQTAIAARLESAENRVKLRSLMTNERVDDALTRFDQLERRVDYAEGRAEALKISDQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCC
SGTPSLADEIDALAGSDKVDEELEEMKRALGMGGKDDKQED
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SRLEAEVEKLRRSPTPTQGKQSEPFTASEWADADKVNAKVNEFLSGAPFMGIFSRTRDI
CHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
IAANFNDMLDQADDPEKMIRMIILEMEETLVEVRASAARTIADQKEMHRHTVKLDKLQAD
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WAEKAQLALSKDREDLARAALVEKKKAADMSDQLKQEIAVLDDALRAYEQDIQKLQGRLR
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EARSRQTAIAARLESAENRVKLRSLMTNERVDDALTRFDQLERRVDYAEGRAEALKISDQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCC
SGTPSLADEIDALAGSDKVDEELEEMKRALGMGGKDDKQED
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]