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The map label for this gene is leuD

Identifier: 85373487

GI number: 85373487

Start: 696604

End: 697206

Strand: Reverse

Name: leuD

Synonym: ELI_03300

Alternate gene names: 85373487

Gene position: 697206-696604 (Counterclockwise)

Preceding gene: 85373488

Following gene: 85373486

Centisome position: 22.84

GC content: 59.2

Gene sequence:

>603_bases
ATGGAGGCCTTGAGCCAGATCGAAGGGCGCGCGATTCCCTATGGCGCGAAGAATGTCGACACCGACGTCATCGTCCCTGC
ACACTGGCTCAAGACGATCACCCGCGAAGGACTGGGGCGCGGCGCGTTCGAGACGTTGCGCGAAGTCCCGGACAATATCT
TCGACAGCGAGGAATTCGCCGGAGCGCCGATCCTGATCGCCGGCGACAATTTCGGTTGCGGATCGAGTCGCGAACACGCG
GCATGGGCCTTGCTAGATCTCGGCATAAGGGCGGTGATCGCGCCGAGCTTCTCCGACATCTTCTCCAGCAATGCTTTCAA
GAACGGTATCGTAACGGTCGTCTTATCGCAGTACGAGGTCGACCGATTGATGGAGGTCGCGCAAAGCGATCCGATCGCGG
TCGACCTCGAAACGCAGACTGTCGTAACGCCCTTTCAGGACCGGTTCCCGTTCGAAATCGACCCGTTTCGCAAGCACTGT
CTGCTCAATGGATTGGACGAAGTCGGCCTGACCCTCACCCGCGATGCCGCCATCGCAGACTATGAATCGCGCCAAAAGAC
ACAAACGCCCTGGCTTACAACAGGAACGGAGAGAGCCGCATGA

Upstream 100 bases:

>100_bases
CTATCGCCGCCGCGCTGCTCTATGCGGGCAAGCGCAAGAAGAGAAACGAGCCGACCCAGCCCGGCCCCATCCCCTCGGGC
GAGAACCCGGAGACCGACTG

Downstream 100 bases:

>100_bases
AAGTCCTACGCACCCACGCCGTCGGAGGCCCCGAGACGCTGACACTCGACGAGGTCGAGGTCCCTACCCCGGGCAAGGGC
GAAGTGCTGGTCGATGTGAA

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MEALSQIEGRAIPYGAKNVDTDVIVPAHWLKTITREGLGRGAFETLREVPDNIFDSEEFAGAPILIAGDNFGCGSSREHA
AWALLDLGIRAVIAPSFSDIFSSNAFKNGIVTVVLSQYEVDRLMEVAQSDPIAVDLETQTVVTPFQDRFPFEIDPFRKHC
LLNGLDEVGLTLTRDAAIADYESRQKTQTPWLTTGTERAA

Sequences:

>Translated_200_residues
MEALSQIEGRAIPYGAKNVDTDVIVPAHWLKTITREGLGRGAFETLREVPDNIFDSEEFAGAPILIAGDNFGCGSSREHA
AWALLDLGIRAVIAPSFSDIFSSNAFKNGIVTVVLSQYEVDRLMEVAQSDPIAVDLETQTVVTPFQDRFPFEIDPFRKHC
LLNGLDEVGLTLTRDAAIADYESRQKTQTPWLTTGTERAA
>Mature_200_residues
MEALSQIEGRAIPYGAKNVDTDVIVPAHWLKTITREGLGRGAFETLREVPDNIFDSEEFAGAPILIAGDNFGCGSSREHA
AWALLDLGIRAVIAPSFSDIFSSNAFKNGIVTVVLSQYEVDRLMEVAQSDPIAVDLETQTVVTPFQDRFPFEIDPFRKHC
LLNGLDEVGLTLTRDAAIADYESRQKTQTPWLTTGTERAA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=195, Percent_Identity=45.6410256410256, Blast_Score=172, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6321429, Length=211, Percent_Identity=41.7061611374408, Blast_Score=165, Evalue=5e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_ERYLH (Q2NC39)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_457549.1
- ProteinModelPortal:   Q2NC39
- SMR:   Q2NC39
- STRING:   Q2NC39
- GeneID:   3869207
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_03300
- NMPDR:   fig|314225.3.peg.2421
- eggNOG:   COG0066
- HOGENOM:   HBG304838
- OMA:   DEISITM
- PhylomeDB:   Q2NC39
- ProtClustDB:   PRK01641
- BioCyc:   ELIT314225:ELI_03300-MONOMER
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 21958; Mature: 21958

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEALSQIEGRAIPYGAKNVDTDVIVPAHWLKTITREGLGRGAFETLREVPDNIFDSEEFA
CCCHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHC
GAPILIAGDNFGCGSSREHAAWALLDLGIRAVIAPSFSDIFSSNAFKNGIVTVVLSQYEV
CCCEEEECCCCCCCCCCCHHHHHHHHHHHHHEECCCHHHHHHCCCCCCCEEEEEECHHHH
DRLMEVAQSDPIAVDLETQTVVTPFQDRFPFEIDPFRKHCLLNGLDEVGLTLTRDAAIAD
HHHHHHHCCCCEEEEECCCEEECCCCCCCCCCCCHHHHHHHHCCHHHHCEEEECCCHHHH
YESRQKTQTPWLTTGTERAA
HHHHHCCCCCCEECCCCCCC
>Mature Secondary Structure
MEALSQIEGRAIPYGAKNVDTDVIVPAHWLKTITREGLGRGAFETLREVPDNIFDSEEFA
CCCHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHC
GAPILIAGDNFGCGSSREHAAWALLDLGIRAVIAPSFSDIFSSNAFKNGIVTVVLSQYEV
CCCEEEECCCCCCCCCCCHHHHHHHHHHHHHEECCCHHHHHHCCCCCCCEEEEEECHHHH
DRLMEVAQSDPIAVDLETQTVVTPFQDRFPFEIDPFRKHCLLNGLDEVGLTLTRDAAIAD
HHHHHHHCCCCEEEEECCCEEECCCCCCCCCCCCHHHHHHHHCCHHHHCEEEECCCHHHH
YESRQKTQTPWLTTGTERAA
HHHHHCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA