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The map label for this gene is virB4 [H]

Identifier: 85373472

GI number: 85373472

Start: 679193

End: 681631

Strand: Direct

Name: virB4 [H]

Synonym: ELI_03225

Alternate gene names: 85373472

Gene position: 679193-681631 (Clockwise)

Preceding gene: 85373471

Following gene: 85373473

Centisome position: 22.25

GC content: 65.68

Gene sequence:

>2439_bases
ATGAGCAAGTGGATCGGCCCCGCCGCGATGAGCGCGAAGGAAGCCAGCGCCGGCGACCGCCTTCCTTATGCGCGCTTGGT
CGACGCAAACACACTGCAATTGCGCGATGGCTCGCTCATGATGGCGATCCAGGTGCCGGGCCTGCTGTTCGAGACCGAGG
ACAGCGACGCGCTCAACGCCCATGCGGCGACGCGCGAAGTCGTGCTGCGCTCGACACTGGATTCGCGCTTCGTGCTCTAT
CACCACGTCATTCGCCGCAGGGTCGCGGTGGAGCTGGATGCCGAGTTTCCCGACCCGCTAAGCCGCCACATCGATGCGCG
GTGGAAGGAACGGCTGGGGTCCGGCTCGCTGTTTATCAACGACCAGTTTGTAACCCTCGTGCGCCGTCCCGCGCGTGGCA
AGGCCGGCCTTGCCGACCGCGCGAGCCGATGGTTCAGCCGCCGCAAGGAAGAGCTTGAGGCCGATCCGCGCGAACTGCGC
TCGCTGCAGGCTGCCGCAACCGGGTTGATCGCCTCGCTGCAAGCCTATGGTGCGAGCCTGCTGGGGGAATATCAGGGGCC
TTCCGGCGGCACCAACAGCGAGATATTGGAGCTCCTTTCCGCGCTCTACAATGGCGAGATGAAGCCGGTGCGCCGCCCTG
CCGAGGACGTCGATATCGGCCAAATGCTGCCCTATCGCCGCGCCAGTTTCGGACTCGACGCGATGGAGCAGCGCGGGGCG
GGCGATCCCGATTTCTCCGCCATCCTCAGCCTCAAGGATTATCCCGAAGCGACCAGCCCCGGCCTGCTCGATAGCCTGCT
GCGCCTGCCTTACGAGATGATCGTGACGGAGACCTATGCACCGCAAGAGCGGACCACCGCGCGCGAGCGGATCGACCTGT
CGCTGCGGCGCCTGCGCTCGGTCGACGAGGAAGCCACCGCCGAACGCGCCGACATGCAGAACGCGCGCGATGCGCTGGGC
AATGGCGCGGTCGGGTTCGGCGATCATCACCTAAGCGTGCTGGTGCGCGAGACCACGCTTCCCCGGCTCGACGATGCCAG
CGCCGCCTGTGCCGCTGCGCTGGCCGATACCGGCGCGGTGGTGGTGCGCGAAGACACCAATCTCGAACCGGCCTTCTGGG
CGCAATTGCCCGGCAATGAAGCCTATATCGTGCGCCGCGCGATGATCAGTTCGGCCAATATGGCGAGCTTCGGTTCGTTG
CATGGGTTTGCGCTGGGGCAGGCGGCGGGCAACCATTGGGGCGATGCCGTCACGCTGCTGGAAACGACCAGCGCGACGCC
GTTCTTCTTCAACTTCCACAATGGCGACCTGGGCAATTTCTCCGTCATCGGGCCGAGCGGTTCGGGCAAGACGGTGGTGA
TGAATTTCCTCGCCGCGCAGGCGCAGAAATTCGCACCGCGCACAATCCTGTTCGACAAGGATCGCGGCGCGGAACTGTTC
GTGCGCGGGATCGGCGGGCGGTACGACCGGATTTATGCCGGAGAGCCGACCGGGTTCAATCCGCTCGCTTTGCCCGATAG
CGCCGCCAACCGCGCTTTCCTGCGCGACTGGCTCGGCGTGCTACTGAAAGCCGAGGGGCCGGAAGAATTGCAGACGATCG
CCCACGCGGTCGACGCCGCCTACGAGAACGATGCCAGCCTGCGCCAACTGCGCAATTTCCGCGAGTTGCTGTCGGGGTCG
CGGCGTCCGGAGCCGGGGGACCTGCCGGATCGCCTGTCGGCCTGGATCCACTCGGGCGAGCATGCATGGCTGTTCGACAA
CCAGCGCGACGAGCTCGACCTCGACAATCGAACGCTCGGCTTCGACATGACGGCGCTGCTGGAAAATCCCAAGCTGCGCA
CGCCGACCATGATGTACCTGTTCCATCGCATCGAGGAACGGCTCGACGGCAACCCGGCGATGATCCTTATCGACGAGGGC
TGGAAGGCGCTCGACGATGAAGTTTTCGCCGCGCGCATCCGCGACTGGCTCAAGACGCTGCGGAAGCGCAACGCGCTGGT
CGGTTTTGCCACGCAGAGCGCTGCCGACGCGCTGGAAAGCCGCATCTCGACTGCGCTGGTCGAGCAGACCGCCACCATGG
TCTTCATGCCCAACAGCCGCGCCCGGGCGGAGGATTATTGCGACGGCTTCGGCCTTACCAGTCACGAACTGGCGCTGATC
CGCTCGCTGCCCGCACATAGCCGCTGCTTCCTCGTGCGCCAGCCCGATGCCAGCGTGGTGGTACGACTCGACCTGTCGAA
TGCGCCCGAGGTGCTGACTGTCCTCTCGGGCCGTGAAAGCAGCGTGCGCCGCCTCGATCTGCTGCGCGAAGCGGTCGGGG
ACAATCCCGCCGACTGGTTCCCCGCACTTACCGGCCATGCCTGGCCGGGTGACCCCTCGGAAGAAACCGAAGCGGGCGCG
GACGTCACCGCCATTTCCTTCAAGCAGGCTGCCGAATGA

Upstream 100 bases:

>100_bases
CACCAAGGTCTCGATGTGTCCGCGGGTGAAGAATTACAAGACGTGGGGGTGCAACAGCTATGCGCCCTGAGTGTGCAGTC
TCGCCGGAAAGAGCATTCGC

Downstream 100 bases:

>100_bases
GCGCGCAGTGCGATCTTGTCCTGTCGCAGGCGGCGGGCGGGATTGCCGCCGCGCTGCAGGCGGTCGATTGCGTTGCTGCC
GAAGTGACCGGCACCGCCTT

Product: type IV secretion system protein B4, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 812; Mature: 811

Protein sequence:

>812_residues
MSKWIGPAAMSAKEASAGDRLPYARLVDANTLQLRDGSLMMAIQVPGLLFETEDSDALNAHAATREVVLRSTLDSRFVLY
HHVIRRRVAVELDAEFPDPLSRHIDARWKERLGSGSLFINDQFVTLVRRPARGKAGLADRASRWFSRRKEELEADPRELR
SLQAAATGLIASLQAYGASLLGEYQGPSGGTNSEILELLSALYNGEMKPVRRPAEDVDIGQMLPYRRASFGLDAMEQRGA
GDPDFSAILSLKDYPEATSPGLLDSLLRLPYEMIVTETYAPQERTTARERIDLSLRRLRSVDEEATAERADMQNARDALG
NGAVGFGDHHLSVLVRETTLPRLDDASAACAAALADTGAVVVREDTNLEPAFWAQLPGNEAYIVRRAMISSANMASFGSL
HGFALGQAAGNHWGDAVTLLETTSATPFFFNFHNGDLGNFSVIGPSGSGKTVVMNFLAAQAQKFAPRTILFDKDRGAELF
VRGIGGRYDRIYAGEPTGFNPLALPDSAANRAFLRDWLGVLLKAEGPEELQTIAHAVDAAYENDASLRQLRNFRELLSGS
RRPEPGDLPDRLSAWIHSGEHAWLFDNQRDELDLDNRTLGFDMTALLENPKLRTPTMMYLFHRIEERLDGNPAMILIDEG
WKALDDEVFAARIRDWLKTLRKRNALVGFATQSAADALESRISTALVEQTATMVFMPNSRARAEDYCDGFGLTSHELALI
RSLPAHSRCFLVRQPDASVVVRLDLSNAPEVLTVLSGRESSVRRLDLLREAVGDNPADWFPALTGHAWPGDPSEETEAGA
DVTAISFKQAAE

Sequences:

>Translated_812_residues
MSKWIGPAAMSAKEASAGDRLPYARLVDANTLQLRDGSLMMAIQVPGLLFETEDSDALNAHAATREVVLRSTLDSRFVLY
HHVIRRRVAVELDAEFPDPLSRHIDARWKERLGSGSLFINDQFVTLVRRPARGKAGLADRASRWFSRRKEELEADPRELR
SLQAAATGLIASLQAYGASLLGEYQGPSGGTNSEILELLSALYNGEMKPVRRPAEDVDIGQMLPYRRASFGLDAMEQRGA
GDPDFSAILSLKDYPEATSPGLLDSLLRLPYEMIVTETYAPQERTTARERIDLSLRRLRSVDEEATAERADMQNARDALG
NGAVGFGDHHLSVLVRETTLPRLDDASAACAAALADTGAVVVREDTNLEPAFWAQLPGNEAYIVRRAMISSANMASFGSL
HGFALGQAAGNHWGDAVTLLETTSATPFFFNFHNGDLGNFSVIGPSGSGKTVVMNFLAAQAQKFAPRTILFDKDRGAELF
VRGIGGRYDRIYAGEPTGFNPLALPDSAANRAFLRDWLGVLLKAEGPEELQTIAHAVDAAYENDASLRQLRNFRELLSGS
RRPEPGDLPDRLSAWIHSGEHAWLFDNQRDELDLDNRTLGFDMTALLENPKLRTPTMMYLFHRIEERLDGNPAMILIDEG
WKALDDEVFAARIRDWLKTLRKRNALVGFATQSAADALESRISTALVEQTATMVFMPNSRARAEDYCDGFGLTSHELALI
RSLPAHSRCFLVRQPDASVVVRLDLSNAPEVLTVLSGRESSVRRLDLLREAVGDNPADWFPALTGHAWPGDPSEETEAGA
DVTAISFKQAAE
>Mature_811_residues
SKWIGPAAMSAKEASAGDRLPYARLVDANTLQLRDGSLMMAIQVPGLLFETEDSDALNAHAATREVVLRSTLDSRFVLYH
HVIRRRVAVELDAEFPDPLSRHIDARWKERLGSGSLFINDQFVTLVRRPARGKAGLADRASRWFSRRKEELEADPRELRS
LQAAATGLIASLQAYGASLLGEYQGPSGGTNSEILELLSALYNGEMKPVRRPAEDVDIGQMLPYRRASFGLDAMEQRGAG
DPDFSAILSLKDYPEATSPGLLDSLLRLPYEMIVTETYAPQERTTARERIDLSLRRLRSVDEEATAERADMQNARDALGN
GAVGFGDHHLSVLVRETTLPRLDDASAACAAALADTGAVVVREDTNLEPAFWAQLPGNEAYIVRRAMISSANMASFGSLH
GFALGQAAGNHWGDAVTLLETTSATPFFFNFHNGDLGNFSVIGPSGSGKTVVMNFLAAQAQKFAPRTILFDKDRGAELFV
RGIGGRYDRIYAGEPTGFNPLALPDSAANRAFLRDWLGVLLKAEGPEELQTIAHAVDAAYENDASLRQLRNFRELLSGSR
RPEPGDLPDRLSAWIHSGEHAWLFDNQRDELDLDNRTLGFDMTALLENPKLRTPTMMYLFHRIEERLDGNPAMILIDEGW
KALDDEVFAARIRDWLKTLRKRNALVGFATQSAADALESRISTALVEQTATMVFMPNSRARAEDYCDGFGLTSHELALIR
SLPAHSRCFLVRQPDASVVVRLDLSNAPEVLTVLSGRESSVRRLDLLREAVGDNPADWFPALTGHAWPGDPSEETEAGAD
VTAISFKQAAE

Specific function: The type IV secretion system VirB/VirD4 is a major virulence determinant for subversion of human endothelial cell (HEC) function. VirB-dependent changes of HEC include massive cytoskeletal rearrangements, a proinflammatory activation by nuclear factor NF-

COG id: COG3451

COG function: function code U; Type IV secretory pathway, VirB4 components

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trbE/virB4 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004346
- InterPro:   IPR018145 [H]

Pfam domain/function: PF03135 CagE_TrbE_VirB [H]

EC number: NA

Molecular weight: Translated: 89301; Mature: 89169

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKWIGPAAMSAKEASAGDRLPYARLVDANTLQLRDGSLMMAIQVPGLLFETEDSDALNA
CCCCCCCHHCCCCCCCCCCCCCCCEEECCCEEEEECCCEEEEEECCCEEEECCCCCCHHH
HAATREVVLRSTLDSRFVLYHHVIRRRVAVELDAEFPDPLSRHIDARWKERLGSGSLFIN
HHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEC
DQFVTLVRRPARGKAGLADRASRWFSRRKEELEADPRELRSLQAAATGLIASLQAYGASL
CHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGEYQGPSGGTNSEILELLSALYNGEMKPVRRPAEDVDIGQMLPYRRASFGLDAMEQRGA
HHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCHHHHCCCHHHHHCCC
GDPDFSAILSLKDYPEATSPGLLDSLLRLPYEMIVTETYAPQERTTARERIDLSLRRLRS
CCCCHHHHEEHHCCCCCCCCHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
VDEEATAERADMQNARDALGNGAVGFGDHHLSVLVRETTLPRLDDASAACAAALADTGAV
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCEE
VVREDTNLEPAFWAQLPGNEAYIVRRAMISSANMASFGSLHGFALGQAAGNHWGDAVTLL
EEECCCCCCCCEEEECCCCCCEEHHHHHHCCCCCHHCCCCCCHHHHHHCCCCCCCEEEEE
ETTSATPFFFNFHNGDLGNFSVIGPSGSGKTVVMNFLAAQAQKFAPRTILFDKDRGAELF
EECCCCEEEEEECCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEE
VRGIGGRYDRIYAGEPTGFNPLALPDSAANRAFLRDWLGVLLKAEGPEELQTIAHAVDAA
EEECCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
YENDASLRQLRNFRELLSGSRRPEPGDLPDRLSAWIHSGEHAWLFDNQRDELDLDNRTLG
HCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEC
FDMTALLENPKLRTPTMMYLFHRIEERLDGNPAMILIDEGWKALDDEVFAARIRDWLKTL
CCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
RKRNALVGFATQSAADALESRISTALVEQTATMVFMPNSRARAEDYCDGFGLTSHELALI
HHCCCEEEECHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHCCCCCCHHHHHHH
RSLPAHSRCFLVRQPDASVVVRLDLSNAPEVLTVLSGRESSVRRLDLLREAVGDNPADWF
HCCCCCCEEEEEECCCCEEEEEEECCCCCCEEHEECCCCHHHHHHHHHHHHHCCCCHHHH
PALTGHAWPGDPSEETEAGADVTAISFKQAAE
HHHCCCCCCCCCCCHHHCCCCEEEECHHHHCC
>Mature Secondary Structure 
SKWIGPAAMSAKEASAGDRLPYARLVDANTLQLRDGSLMMAIQVPGLLFETEDSDALNA
CCCCCCHHCCCCCCCCCCCCCCCEEECCCEEEEECCCEEEEEECCCEEEECCCCCCHHH
HAATREVVLRSTLDSRFVLYHHVIRRRVAVELDAEFPDPLSRHIDARWKERLGSGSLFIN
HHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEC
DQFVTLVRRPARGKAGLADRASRWFSRRKEELEADPRELRSLQAAATGLIASLQAYGASL
CHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGEYQGPSGGTNSEILELLSALYNGEMKPVRRPAEDVDIGQMLPYRRASFGLDAMEQRGA
HHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCHHHHCCCHHHHHCCC
GDPDFSAILSLKDYPEATSPGLLDSLLRLPYEMIVTETYAPQERTTARERIDLSLRRLRS
CCCCHHHHEEHHCCCCCCCCHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
VDEEATAERADMQNARDALGNGAVGFGDHHLSVLVRETTLPRLDDASAACAAALADTGAV
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCEE
VVREDTNLEPAFWAQLPGNEAYIVRRAMISSANMASFGSLHGFALGQAAGNHWGDAVTLL
EEECCCCCCCCEEEECCCCCCEEHHHHHHCCCCCHHCCCCCCHHHHHHCCCCCCCEEEEE
ETTSATPFFFNFHNGDLGNFSVIGPSGSGKTVVMNFLAAQAQKFAPRTILFDKDRGAELF
EECCCCEEEEEECCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEE
VRGIGGRYDRIYAGEPTGFNPLALPDSAANRAFLRDWLGVLLKAEGPEELQTIAHAVDAA
EEECCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
YENDASLRQLRNFRELLSGSRRPEPGDLPDRLSAWIHSGEHAWLFDNQRDELDLDNRTLG
HCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEC
FDMTALLENPKLRTPTMMYLFHRIEERLDGNPAMILIDEGWKALDDEVFAARIRDWLKTL
CCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
RKRNALVGFATQSAADALESRISTALVEQTATMVFMPNSRARAEDYCDGFGLTSHELALI
HHCCCEEEECHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHCCCCCCHHHHHHH
RSLPAHSRCFLVRQPDASVVVRLDLSNAPEVLTVLSGRESSVRRLDLLREAVGDNPADWF
HCCCCCCEEEEEECCCCEEEEEEECCCCCCEEHEECCCCHHHHHHHHHHHHHCCCCHHHH
PALTGHAWPGDPSEETEAGADVTAISFKQAAE
HHHCCCCCCCCCCCHHHCCCCEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10749166; 10882236; 7494028 [H]