Click here to switch to the map view.

The map label for this gene is rpoE [H]

Identifier: 85373439

GI number: 85373439

Start: 643438

End: 644073

Strand: Reverse

Name: rpoE [H]

Synonym: ELI_03060

Alternate gene names: 85373439

Gene position: 644073-643438 (Counterclockwise)

Preceding gene: 85373442

Following gene: 85373438

Centisome position: 21.1

GC content: 63.68

Gene sequence:

>636_bases
TTGCGCAGCAAGCGGAGCGGCCAGGAGGGCGAGCAGGGCCATGGCGGTGGGCAGAATTCGGCGCATCATTGTCTCGTTTC
CATTTTTTGCCTTAAGCACGGATTAAACGCACCGCGCCGCTCGTTTAATCCGTGGAAGGCACAGATTCTGACTGAAGGCT
TCGAACAGCAGGTGCTCGTATTGCTGCCGCGTCTGCGGCGCTTTGCGATCGGGCTTGCCGGCTCTCCTGCCGACGGCGAC
GACCTGTGCCAGATGACCCTCGAGCGCGCCCTCACCCGCCGGGACCAATGGCAGGAAGGGACACGGCTCGACAGCTGGAT
GTACCGGATCATGCGCAATATCTTTGTCGACGAGAAACGCAGCGGCGCGCGCCGCGCCCAGACCTTCGTGGACGCGGAGG
CCGGCCTGTTGGTCGGCTCCGACGGCGCACAGGAATCTGTCGTCGAGCTGTCCAACGTAGACCGCGCGATGCAGCAATTG
CCCGAAGACCAGCGCGAAGCCGTGCTGCTCGTGATGGTTGAAGGCTATGCCTACAAAGAGGCTGCCGAAATCGTCGGTTG
TCCCGTCGGCACGCTCAACTCGCGGCTGGTCCGCGGGCGCGACGCCTTGCTCACCCTGCTGGAGGACGCGGCATGA

Upstream 100 bases:

>100_bases
GCGCGCTTGCCTCGACGTCGCGCAATGCGTCGCGCGCGGTGTCGGTCACCCCGCCCAGTACGCCGCCTGGATCTGGCACC
TGCACCCCCGGCAACGCCAC

Downstream 100 bases:

>100_bases
GCGTGACCGACCAACAGATCGCGGCCTATGCCGATGGCGAATTGACCGGCGACGCGAAAGCGCAGGTCGAAGCGGCTGTT
GCCGCCGACCCCACGCTTGC

Product: DNA-directed RNA polymerase specialized sigma subunit

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MRSKRSGQEGEQGHGGGQNSAHHCLVSIFCLKHGLNAPRRSFNPWKAQILTEGFEQQVLVLLPRLRRFAIGLAGSPADGD
DLCQMTLERALTRRDQWQEGTRLDSWMYRIMRNIFVDEKRSGARRAQTFVDAEAGLLVGSDGAQESVVELSNVDRAMQQL
PEDQREAVLLVMVEGYAYKEAAEIVGCPVGTLNSRLVRGRDALLTLLEDAA

Sequences:

>Translated_211_residues
MRSKRSGQEGEQGHGGGQNSAHHCLVSIFCLKHGLNAPRRSFNPWKAQILTEGFEQQVLVLLPRLRRFAIGLAGSPADGD
DLCQMTLERALTRRDQWQEGTRLDSWMYRIMRNIFVDEKRSGARRAQTFVDAEAGLLVGSDGAQESVVELSNVDRAMQQL
PEDQREAVLLVMVEGYAYKEAAEIVGCPVGTLNSRLVRGRDALLTLLEDAA
>Mature_211_residues
MRSKRSGQEGEQGHGGGQNSAHHCLVSIFCLKHGLNAPRRSFNPWKAQILTEGFEQQVLVLLPRLRRFAIGLAGSPADGD
DLCQMTLERALTRRDQWQEGTRLDSWMYRIMRNIFVDEKRSGARRAQTFVDAEAGLLVGSDGAQESVVELSNVDRAMQQL
PEDQREAVLLVMVEGYAYKEAAEIVGCPVGTLNSRLVRGRDALLTLLEDAA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=140, Percent_Identity=31.4285714285714, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014293
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 23437; Mature: 23437

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSKRSGQEGEQGHGGGQNSAHHCLVSIFCLKHGLNAPRRSFNPWKAQILTEGFEQQVLV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
LLPRLRRFAIGLAGSPADGDDLCQMTLERALTRRDQWQEGTRLDSWMYRIMRNIFVDEKR
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHH
SGARRAQTFVDAEAGLLVGSDGAQESVVELSNVDRAMQQLPEDQREAVLLVMVEGYAYKE
CHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHEEEEEEECCCCHHH
AAEIVGCPVGTLNSRLVRGRDALLTLLEDAA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRSKRSGQEGEQGHGGGQNSAHHCLVSIFCLKHGLNAPRRSFNPWKAQILTEGFEQQVLV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
LLPRLRRFAIGLAGSPADGDDLCQMTLERALTRRDQWQEGTRLDSWMYRIMRNIFVDEKR
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHH
SGARRAQTFVDAEAGLLVGSDGAQESVVELSNVDRAMQQLPEDQREAVLLVMVEGYAYKE
CHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHEEEEEEECCCCHHH
AAEIVGCPVGTLNSRLVRGRDALLTLLEDAA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]