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The map label for this gene is lepB [H]

Identifier: 85373368

GI number: 85373368

Start: 578481

End: 579371

Strand: Reverse

Name: lepB [H]

Synonym: ELI_02705

Alternate gene names: 85373368

Gene position: 579371-578481 (Counterclockwise)

Preceding gene: 85373369

Following gene: 85373367

Centisome position: 18.98

GC content: 61.95

Gene sequence:

>891_bases
ATGACGACGAGTACCCCCACCCAAGTGACCGATACCGACATCAAAAAGAGCGAAGAAAAGGTCAACTGGTTCGCCGAATT
GCGCGGGCTCGGGCTGATGCTCTTCGCGGTGCTGATGTTCCACAGCTTTGTCGCCAAGCCGTTCTACATTCCTTCGCAAT
CGATGATGCCGAACTTGCTGGTCGGAGACCGGCTTGTGGTGACCAAATATCCCTATGGCTACAATTGGTCCTCGGTCAGC
TTCCACCTGTTGCCGCGTGGCGACTGGCGCGTGTTCGGATCGACGCCCGAGTACGGGGATATCGTTATCCCCGTACACCC
GGAGCGCAACGAGGATTACATCAAACGCGTGGTCGGCCTGCCGGGCGACACGATCGAAGTGCGCGACGGGCGGATCATTC
TCAACGGCACCGAAGTGCGCCGCGAGATGGAGCCTTCGCTAGACCTGCCGTTCGATCTCAACAACGATTGCAGCGAGATG
GAATTCGAAGTCGTTGCAACGCAGGGCGGCGAAGAGGCCTGCCGCGTCCCGACCTATCGCGAAACCATGCCCAACGGCGT
GTCCTATCTTATCATCGACGCGCCCGACGTGCTCGGGCTCGAGAGCTATCCGGCGACGGTCATTCCCGACGATCACGTCT
TTGTGATGGGCGACAATCGCGATCATTCGGCCGACAGCCGCGCGCCGGTCTCGCCGATCGGCGGCGGGCTCGGCGGGCCG
GTGCCGCTGGCCAATATCGGCGGGCGCGCCGAATTCATAACTTTCTCGCTCGACGGCACCGCCAACTGGAATCCTTTCAG
CTGGTGGAGCGCCTTGCGCGAGGGCCGCGCATGGTCGACCCTGCGGCCGGCCAAGGCCGAAACGACCGCAACAGCCGACG
GGGAAACGTGA

Upstream 100 bases:

>100_bases
CGGAATTGCTACCGGATGGCCATGAGGCATCCATTCATCTCACCCTCACCGACGATCACCCATGGGCGCAGGCCTTCGTG
ATCATCGAGGCCCACCGGAT

Downstream 100 bases:

>100_bases
GCGAGCGCGACAACACCGATTATTCCGACCCGGACCACGTCGAGGATCTCGGCGCCAGCCCTTCCTATATCGATAATCCG
CAGCTTCGGCTGGAGGCCTA

Product: signal peptidase I

Products: NA

Alternate protein names: SPase I; Leader peptidase I [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MTTSTPTQVTDTDIKKSEEKVNWFAELRGLGLMLFAVLMFHSFVAKPFYIPSQSMMPNLLVGDRLVVTKYPYGYNWSSVS
FHLLPRGDWRVFGSTPEYGDIVIPVHPERNEDYIKRVVGLPGDTIEVRDGRIILNGTEVRREMEPSLDLPFDLNNDCSEM
EFEVVATQGGEEACRVPTYRETMPNGVSYLIIDAPDVLGLESYPATVIPDDHVFVMGDNRDHSADSRAPVSPIGGGLGGP
VPLANIGGRAEFITFSLDGTANWNPFSWWSALREGRAWSTLRPAKAETTATADGET

Sequences:

>Translated_296_residues
MTTSTPTQVTDTDIKKSEEKVNWFAELRGLGLMLFAVLMFHSFVAKPFYIPSQSMMPNLLVGDRLVVTKYPYGYNWSSVS
FHLLPRGDWRVFGSTPEYGDIVIPVHPERNEDYIKRVVGLPGDTIEVRDGRIILNGTEVRREMEPSLDLPFDLNNDCSEM
EFEVVATQGGEEACRVPTYRETMPNGVSYLIIDAPDVLGLESYPATVIPDDHVFVMGDNRDHSADSRAPVSPIGGGLGGP
VPLANIGGRAEFITFSLDGTANWNPFSWWSALREGRAWSTLRPAKAETTATADGET
>Mature_295_residues
TTSTPTQVTDTDIKKSEEKVNWFAELRGLGLMLFAVLMFHSFVAKPFYIPSQSMMPNLLVGDRLVVTKYPYGYNWSSVSF
HLLPRGDWRVFGSTPEYGDIVIPVHPERNEDYIKRVVGLPGDTIEVRDGRIILNGTEVRREMEPSLDLPFDLNNDCSEME
FEVVATQGGEEACRVPTYRETMPNGVSYLIIDAPDVLGLESYPATVIPDDHVFVMGDNRDHSADSRAPVSPIGGGLGGPV
PLANIGGRAEFITFSLDGTANWNPFSWWSALREGRAWSTLRPAKAETTATADGET

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family [H]

Homologues:

Organism=Escherichia coli, GI1788921, Length=290, Percent_Identity=32.4137931034483, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019757
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056 [H]

Pfam domain/function: PF00717 Peptidase_S24 [H]

EC number: =3.4.21.89 [H]

Molecular weight: Translated: 32778; Mature: 32647

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00760 SPASE_I_2 ; PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSTPTQVTDTDIKKSEEKVNWFAELRGLGLMLFAVLMFHSFVAKPFYIPSQSMMPNLL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEECCCHHCCCCEE
VGDRLVVTKYPYGYNWSSVSFHLLPRGDWRVFGSTPEYGDIVIPVHPERNEDYIKRVVGL
ECCEEEEEECCCCCCCCCEEEEEEECCCEEEECCCCCCCCEEEEECCCCCHHHHHHHHCC
PGDTIEVRDGRIILNGTEVRREMEPSLDLPFDLNNDCSEMEFEVVATQGGEEACRVPTYR
CCCEEEEECCEEEEEHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHCCCCHH
ETMPNGVSYLIIDAPDVLGLESYPATVIPDDHVFVMGDNRDHSADSRAPVSPIGGGLGGP
HHCCCCCEEEEEECCCCCCCCCCCCEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCC
VPLANIGGRAEFITFSLDGTANWNPFSWWSALREGRAWSTLRPAKAETTATADGET
CCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTSTPTQVTDTDIKKSEEKVNWFAELRGLGLMLFAVLMFHSFVAKPFYIPSQSMMPNLL
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEECCCHHCCCCEE
VGDRLVVTKYPYGYNWSSVSFHLLPRGDWRVFGSTPEYGDIVIPVHPERNEDYIKRVVGL
ECCEEEEEECCCCCCCCCEEEEEEECCCEEEECCCCCCCCEEEEECCCCCHHHHHHHHCC
PGDTIEVRDGRIILNGTEVRREMEPSLDLPFDLNNDCSEMEFEVVATQGGEEACRVPTYR
CCCEEEEECCEEEEEHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHCCCCHH
ETMPNGVSYLIIDAPDVLGLESYPATVIPDDHVFVMGDNRDHSADSRAPVSPIGGGLGGP
HHCCCCCEEEEEECCCCCCCCCCCCEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCC
VPLANIGGRAEFITFSLDGTANWNPFSWWSALREGRAWSTLRPAKAETTATADGET
CCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA