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The map label for this gene is 85373327

Identifier: 85373327

GI number: 85373327

Start: 539773

End: 541353

Strand: Reverse

Name: 85373327

Synonym: ELI_02500

Alternate gene names: NA

Gene position: 541353-539773 (Counterclockwise)

Preceding gene: 85373329

Following gene: 85373326

Centisome position: 17.74

GC content: 63.69

Gene sequence:

>1581_bases
ATGGATGCGTCGTTCGTGGCGCTCGAAACGCCGACCTCGCCGATGCACATCGGCTCGATCCTGATCTACAATCCGGAGAC
GGCACCGGGCGGCTTCGTGCGGTTCAAGGATATCCTGAGCTTCTTCGAAAGCCGGATGCAGCTGTCCACCACGATCCGCC
AGCGCATGGTGCGGGTGCCGTTCGATCTCGACTATCCCTACTGGATCGAGGACCCGGAATTCGACCTCGAATACCATATC
CGCCACATCGCCCTGCCCAAGCCGGGGGACTGGCGGCAATTGTGCATCCAGGCTGCGCGGATCTTCGCCCGGCCGCTCGA
CCTCGAAAAGCCGCCGTGGGAATTCACCGTGGTCGAGGGGCTGGACAATATCCCCGGCGTCGCGCCCGGCAGCTATGCCA
TGGTCACCAAGGTCCACCACGCCGCGATCGACGGGATGAGCGGGATCGACCTGATGGAGGCGACGCACACGCTCGACCCC
AACGATCCGCCGCCCAACACGCCCGACACCTGGAAGCCGGAGCGAATACCCGGCCCCGCCGAATTGCTCGGCAAGAGCTA
CGTCAACACGCTGCTCAACCCGGCCAAGCAGCTGGAGGTCGCGGCCAAGGCCGTGCCGGGCATCGCCAAGGCGGTGACGG
GGCTGGTGAAGAAGGAATTCTCGCTCAGCAGCGACATGGTCGCCCCGCGCACACGCTTCAATCGCAAGATTTCGCCGCAC
CGCGTGGTCGAGGGCCGCAGCTTCCCGCTCGAGGACATCAAGGCGATCCGCGCGCTCGCGCCGGGGAGCAAGGTCAACGA
CGTATTCCTCTCCATCATCGGCGGGGGACTGCGCAAATACCTGGCTGCGAAGGACGACCTGCCCAAGGCGACGCTGACGG
CGATGGCCCCGATCTCGGTCCGCTCGAAGGACGAGAAGAACGACATGGGCAACCAGGTCGCGGCAATGATCGCGCCGCTC
GGCACGCATATCGAGGATCCGGCCGAGCGCATCGCCTGGGTCCATTCGAAAACGACCAATTCCAAGGCCATGACCGACGC
CATGGGCGCGCGCAACATGACCGAGATCAGCAAGGTCAGCCCGGCGCTCTACATGTCGCTCGGGGCGCAGCTCTATACGC
GCCTGGGCCTCGCCAATCGCGGCGTCGGCCCGATCTTCTCGACCGTCGTCACCAATGTCCCCGGCCCGCCGGTGCCGATC
TATTCCAGCGGCGCGCGGCTTGAAAGCATGATGGGGCTGATCTGCCTGACCGACGGCATGGGGCTCGCGCATACGGTCCA
GAGCTATGTCGGCGAAGCGACGATCTGCTTCACCGCCTGCCGCGAATTGCTGCCCGATCCGGATTTCTACATCGAATGCA
TCGAGGAAAGCTTCGAGGAACTGCGCGATGCGGCAGCCGTTGTCGCAAAAACGCCCACGGACAAACCCGCGAAGAAGGCA
CCTGCGAAGAAGGCACCTGCGAAGAAGGCTCCGGCAAAAAAGATCGCCGCAAAGCCCAGGACCACCAAATCCAAACCGAA
AACAAAACCGAAAACGGCCGCCAAGAGCCCGTCGGGTAGCAAAGGAAAAACCACCGCATGA

Upstream 100 bases:

>100_bases
CCGCGCCTGTTACCCCTCTGCGATAGACCCGCAAAAGCGGGCGACCGGCACAGGATGCGTGCCGACACACAGGAGGATGC
CTTTGCGACAGCTGCAAGGT

Downstream 100 bases:

>100_bases
CCGCAGCAACCATCGACCAGGATAGCGACCGGATGCCCGAAGCGCGGCCGCCCAGCCGCCTGCTGGCGCTGGCCGAGCCC
GGCCGGGCCATGGGCGAGCT

Product: hypothetical protein

Products: NA

Alternate protein names: Putative triacylglycerol synthase Rv1425/MT1468 [H]

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MDASFVALETPTSPMHIGSILIYNPETAPGGFVRFKDILSFFESRMQLSTTIRQRMVRVPFDLDYPYWIEDPEFDLEYHI
RHIALPKPGDWRQLCIQAARIFARPLDLEKPPWEFTVVEGLDNIPGVAPGSYAMVTKVHHAAIDGMSGIDLMEATHTLDP
NDPPPNTPDTWKPERIPGPAELLGKSYVNTLLNPAKQLEVAAKAVPGIAKAVTGLVKKEFSLSSDMVAPRTRFNRKISPH
RVVEGRSFPLEDIKAIRALAPGSKVNDVFLSIIGGGLRKYLAAKDDLPKATLTAMAPISVRSKDEKNDMGNQVAAMIAPL
GTHIEDPAERIAWVHSKTTNSKAMTDAMGARNMTEISKVSPALYMSLGAQLYTRLGLANRGVGPIFSTVVTNVPGPPVPI
YSSGARLESMMGLICLTDGMGLAHTVQSYVGEATICFTACRELLPDPDFYIECIEESFEELRDAAAVVAKTPTDKPAKKA
PAKKAPAKKAPAKKIAAKPRTTKSKPKTKPKTAAKSPSGSKGKTTA

Sequences:

>Translated_526_residues
MDASFVALETPTSPMHIGSILIYNPETAPGGFVRFKDILSFFESRMQLSTTIRQRMVRVPFDLDYPYWIEDPEFDLEYHI
RHIALPKPGDWRQLCIQAARIFARPLDLEKPPWEFTVVEGLDNIPGVAPGSYAMVTKVHHAAIDGMSGIDLMEATHTLDP
NDPPPNTPDTWKPERIPGPAELLGKSYVNTLLNPAKQLEVAAKAVPGIAKAVTGLVKKEFSLSSDMVAPRTRFNRKISPH
RVVEGRSFPLEDIKAIRALAPGSKVNDVFLSIIGGGLRKYLAAKDDLPKATLTAMAPISVRSKDEKNDMGNQVAAMIAPL
GTHIEDPAERIAWVHSKTTNSKAMTDAMGARNMTEISKVSPALYMSLGAQLYTRLGLANRGVGPIFSTVVTNVPGPPVPI
YSSGARLESMMGLICLTDGMGLAHTVQSYVGEATICFTACRELLPDPDFYIECIEESFEELRDAAAVVAKTPTDKPAKKA
PAKKAPAKKAPAKKIAAKPRTTKSKPKTKPKTAAKSPSGSKGKTTA
>Mature_526_residues
MDASFVALETPTSPMHIGSILIYNPETAPGGFVRFKDILSFFESRMQLSTTIRQRMVRVPFDLDYPYWIEDPEFDLEYHI
RHIALPKPGDWRQLCIQAARIFARPLDLEKPPWEFTVVEGLDNIPGVAPGSYAMVTKVHHAAIDGMSGIDLMEATHTLDP
NDPPPNTPDTWKPERIPGPAELLGKSYVNTLLNPAKQLEVAAKAVPGIAKAVTGLVKKEFSLSSDMVAPRTRFNRKISPH
RVVEGRSFPLEDIKAIRALAPGSKVNDVFLSIIGGGLRKYLAAKDDLPKATLTAMAPISVRSKDEKNDMGNQVAAMIAPL
GTHIEDPAERIAWVHSKTTNSKAMTDAMGARNMTEISKVSPALYMSLGAQLYTRLGLANRGVGPIFSTVVTNVPGPPVPI
YSSGARLESMMGLICLTDGMGLAHTVQSYVGEATICFTACRELLPDPDFYIECIEESFEELRDAAAVVAKTPTDKPAKKA
PAKKAPAKKAPAKKIAAKPRTTKSKPKTKPKTAAKSPSGSKGKTTA

Specific function: Upon expression in E.coli functions very weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has no wax synthase activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR004255 [H]

Pfam domain/function: PF03007 UPF0089 [H]

EC number: =2.3.1.20 [H]

Molecular weight: Translated: 57103; Mature: 57103

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDASFVALETPTSPMHIGSILIYNPETAPGGFVRFKDILSFFESRMQLSTTIRQRMVRVP
CCCCEEEEECCCCCEEECEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
FDLDYPYWIEDPEFDLEYHIRHIALPKPGDWRQLCIQAARIFARPLDLEKPPWEFTVVEG
CCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECC
LDNIPGVAPGSYAMVTKVHHAAIDGMSGIDLMEATHTLDPNDPPPNTPDTWKPERIPGPA
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHH
ELLGKSYVNTLLNPAKQLEVAAKAVPGIAKAVTGLVKKEFSLSSDMVAPRTRFNRKISPH
HHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCC
RVVEGRSFPLEDIKAIRALAPGSKVNDVFLSIIGGGLRKYLAAKDDLPKATLTAMAPISV
CCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCC
RSKDEKNDMGNQVAAMIAPLGTHIEDPAERIAWVHSKTTNSKAMTDAMGARNMTEISKVS
CCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHC
PALYMSLGAQLYTRLGLANRGVGPIFSTVVTNVPGPPVPIYSSGARLESMMGLICLTDGM
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHEECCC
GLAHTVQSYVGEATICFTACRELLPDPDFYIECIEESFEELRDAAAVVAKTPTDKPAKKA
CHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHC
PAKKAPAKKAPAKKIAAKPRTTKSKPKTKPKTAAKSPSGSKGKTTA
CCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDASFVALETPTSPMHIGSILIYNPETAPGGFVRFKDILSFFESRMQLSTTIRQRMVRVP
CCCCEEEEECCCCCEEECEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
FDLDYPYWIEDPEFDLEYHIRHIALPKPGDWRQLCIQAARIFARPLDLEKPPWEFTVVEG
CCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECC
LDNIPGVAPGSYAMVTKVHHAAIDGMSGIDLMEATHTLDPNDPPPNTPDTWKPERIPGPA
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHH
ELLGKSYVNTLLNPAKQLEVAAKAVPGIAKAVTGLVKKEFSLSSDMVAPRTRFNRKISPH
HHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCC
RVVEGRSFPLEDIKAIRALAPGSKVNDVFLSIIGGGLRKYLAAKDDLPKATLTAMAPISV
CCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCC
RSKDEKNDMGNQVAAMIAPLGTHIEDPAERIAWVHSKTTNSKAMTDAMGARNMTEISKVS
CCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHC
PALYMSLGAQLYTRLGLANRGVGPIFSTVVTNVPGPPVPIYSSGARLESMMGLICLTDGM
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHEECCC
GLAHTVQSYVGEATICFTACRELLPDPDFYIECIEESFEELRDAAAVVAKTPTDKPAKKA
CHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHC
PAKKAPAKKAPAKKIAAKPRTTKSKPKTKPKTAAKSPSGSKGKTTA
CCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]