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The map label for this gene is cysG [H]

Identifier: 85373231

GI number: 85373231

Start: 449613

End: 450425

Strand: Reverse

Name: cysG [H]

Synonym: ELI_02020

Alternate gene names: 85373231

Gene position: 450425-449613 (Counterclockwise)

Preceding gene: 85373232

Following gene: 85373229

Centisome position: 14.76

GC content: 65.81

Gene sequence:

>813_bases
ATGCCGAGCGCGTGCCCGAATGGCTGGCCTGATCCGGCCATGATCGGCTCGCTGCCTCTCTTCCACCGGGTACGCGGCAA
ACGCGTCGTGGTGGTCGGTGAAGGCGAGATGGGCGAAGCCAAGACCCGGCTCGTCGAACGCGCGGGCGGAATTCCTTGCA
GCGAAGCCGAGGCTCATCACGCGCATCTCGCTTTCGTAGCACTGGAGGATGAGCGGGAAGCCAGGGCAGTGCACCGGCGG
CTCAAGGCAAAAGGCCTGCTGGTGAATGTTGCCGATATGCCCGCGCTTTGCGATTTCACCACGCCCAGCATCCTGGATCG
CGAGCCGGTGCTGGTCGCGGTTTCGACGTCCGGCGCTTCTGCCGGGCTCGCCAAGCATTTGCGCTTGCGGCTTGAGCGAT
TGCTGCCACAAAGCCTTGGTGCGCTTGCAAGCAAGCTGGCGGCATCGCGCGATGCGATGCGGGACCGGTTTCCTTCCGGC
GACCAGCGCCGTCGCGCAGTAGACGCCGCGCTCAGCGAGGGCGGCATTCTCGACCCGTTCGAGAGCGCCGCAGCGGATCG
GGTGGAGGCATGGCTCGATGGCAACGCCGCGCCGGACGCCATCCGGGCCGAAACCATCGCACTGGCCAGCAACGATCCCG
AAGACCTCACACTCAAGCAAGCGCGCCTGCTTGGCGTTGCAGATGTGGTCCTGCATGACGCGGGCATAGATGCCGCAATT
CTCGACCGATCGCGCGCCGACGCCTTGCGCCGCGAATTGCCTCAGGACCCTACCGAGTTCGAGGGCCTCGTCGTCGTGCT
CACACGCGCTTGA

Upstream 100 bases:

>100_bases
GCGAGCAGCTACAACTCGCGCGGTTTCGTCGCAGAGGTGCTGGTCGACGGGGACCAGTTCGCTGTCGTCGCCGACCGGAT
CGATGCGGGCGCGATCATGG

Downstream 100 bases:

>100_bases
GTTCGGTCGCGCGGTTTCAGACCAACCGTCCGGCGTCCTTACCCACTTCTGCAAAATAGCGCGCCATGGCATCGGCGGCA
TTGTCGCTCAGCGCGATGAA

Product: siroheme synthase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MPSACPNGWPDPAMIGSLPLFHRVRGKRVVVVGEGEMGEAKTRLVERAGGIPCSEAEAHHAHLAFVALEDEREARAVHRR
LKAKGLLVNVADMPALCDFTTPSILDREPVLVAVSTSGASAGLAKHLRLRLERLLPQSLGALASKLAASRDAMRDRFPSG
DQRRRAVDAALSEGGILDPFESAAADRVEAWLDGNAAPDAIRAETIALASNDPEDLTLKQARLLGVADVVLHDAGIDAAI
LDRSRADALRRELPQDPTEFEGLVVVLTRA

Sequences:

>Translated_270_residues
MPSACPNGWPDPAMIGSLPLFHRVRGKRVVVVGEGEMGEAKTRLVERAGGIPCSEAEAHHAHLAFVALEDEREARAVHRR
LKAKGLLVNVADMPALCDFTTPSILDREPVLVAVSTSGASAGLAKHLRLRLERLLPQSLGALASKLAASRDAMRDRFPSG
DQRRRAVDAALSEGGILDPFESAAADRVEAWLDGNAAPDAIRAETIALASNDPEDLTLKQARLLGVADVVLHDAGIDAAI
LDRSRADALRRELPQDPTEFEGLVVVLTRA
>Mature_269_residues
PSACPNGWPDPAMIGSLPLFHRVRGKRVVVVGEGEMGEAKTRLVERAGGIPCSEAEAHHAHLAFVALEDEREARAVHRRL
KAKGLLVNVADMPALCDFTTPSILDREPVLVAVSTSGASAGLAKHLRLRLERLLPQSLGALASKLAASRDAMRDRFPSGD
QRRRAVDAALSEGGILDPFESAAADRVEAWLDGNAAPDAIRAETIALASNDPEDLTLKQARLLGVADVVLHDAGIDAAIL
DRSRADALRRELPQDPTEFEGLVVVLTRA

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG1648

COG function: function code H; Siroheme synthase (precorrin-2 oxidase/ferrochelatase domain)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=265, Percent_Identity=28.6792452830189, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 28867; Mature: 28736

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSACPNGWPDPAMIGSLPLFHRVRGKRVVVVGEGEMGEAKTRLVERAGGIPCSEAEAHH
CCCCCCCCCCCHHHHCCCHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCC
AHLAFVALEDEREARAVHRRLKAKGLLVNVADMPALCDFTTPSILDREPVLVAVSTSGAS
EEEEEEEECCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCH
AGLAKHLRLRLERLLPQSLGALASKLAASRDAMRDRFPSGDQRRRAVDAALSEGGILDPF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCH
ESAAADRVEAWLDGNAAPDAIRAETIALASNDPEDLTLKQARLLGVADVVLHDAGIDAAI
HHHHHHHHHHHHCCCCCCCHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
LDRSRADALRRELPQDPTEFEGLVVVLTRA
HHHHHHHHHHHHCCCCCCCCCCEEEEEECC
>Mature Secondary Structure 
PSACPNGWPDPAMIGSLPLFHRVRGKRVVVVGEGEMGEAKTRLVERAGGIPCSEAEAHH
CCCCCCCCCCHHHHCCCHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCC
AHLAFVALEDEREARAVHRRLKAKGLLVNVADMPALCDFTTPSILDREPVLVAVSTSGAS
EEEEEEEECCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCH
AGLAKHLRLRLERLLPQSLGALASKLAASRDAMRDRFPSGDQRRRAVDAALSEGGILDPF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCH
ESAAADRVEAWLDGNAAPDAIRAETIALASNDPEDLTLKQARLLGVADVVLHDAGIDAAI
HHHHHHHHHHHHCCCCCCCHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
LDRSRADALRRELPQDPTEFEGLVVVLTRA
HHHHHHHHHHHHCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA