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The map label for this gene is dhbE [H]

Identifier: 85373227

GI number: 85373227

Start: 446158

End: 447783

Strand: Reverse

Name: dhbE [H]

Synonym: ELI_02000

Alternate gene names: 85373227

Gene position: 447783-446158 (Counterclockwise)

Preceding gene: 85373229

Following gene: 85373225

Centisome position: 14.67

GC content: 63.96

Gene sequence:

>1626_bases
ATGATCTGGCATTTTGCCGACCTGTTCGAGAAGATCGCTTCGCTGGTGCCCGACAATGTCGCGCTGATCGAAGGCGATGC
GAAACGCACTTGGCGCGAATACGAGGATCGCGCGTCGCGGCTGGCGGCGGCGCTGGTCGAGCACGGGATCGAGCCCGATG
CCAAGGTCGCGATCTACGGGCACAACTCCTCCGCGTTCCTCGAAGCGCAATTCGCTGTGTTCAAGGCGCGCGCGGTGCCG
ATCAATGTCAATTACCGCTATGTCGACGACGAACTGGTCTACCTGTTCGACAATGCCGATGTCGATGCGGTGTTCTTCGA
TGCCCGCTTCGCACCGCGCCTCGCCGCAATTCGCGACAAGCTGCCCAAGCTCAAGCTGGCGATCCAGATCGAGGATGGCA
GCGGCGAAAAGCTCGAAGGCGCCAAGGACCTGGAAGCGGTGACCGCCGGGCACGACCCACTCCCGCGCCTCGCCTACAGC
GAAGACGATCATTACATGGTTTACACCGGAGGCACGACGGGCATGCCTAAGGGCGTGGTGTACCGGCAGGGCGATTTCGT
GAAAGCGATCGCGGGTTTCATCCTTGGGCCGGATATCGAGCCATCTAAGGAAATCCTGCTCGGCGCGGTCAAGCAATTGG
CGGCAGCGGGCATGTCGCCGATCGCCTTCCCCGCCTGCCCGCTGATGCACGGCACCGGTATGTGGCTCGGCGGGTTCGCC
GCGCATACGGCGGGCGGCGCGGTCGCGACCTTGCGCGACGAGAAGTTCGATCCCGACCGGACGTGGGAATATGCCGCCAA
GGTCGGCGCCAATGTGATCGTCGTGGTCGGCGATGCCTTTGCCAAGCCGCTGCTGGCGTCGCTGCGCGCGGCGGCAGAGC
GCGGCGAGCCCTATGACCTCTCGAGGCTGCAGGCGATCGTTTCTTCGGGCGCGATGTTCTCCGCCGAGACCAAGCTCGGC
CTGCTCGAACATCTCGACATCGAGATCCGCGACGCCATCGGTTCGACCGAGGGCAGCATGGGCTCCTCCATCACATCGCG
CGCCGCACCGCCCAGCGAGACGGCCAAGTTCGAACTCGGCAAGGACACCAAGGTATTCGACGAGAACGACGAAGAAGTCG
TGCCCGGGTCGGACGCGATCGGCATGATCGCCAATGGCGGCTTCACGCCGATCGCCTATTACAAGGACCCGGAAAAGAGC
GCCAAGACGTTCCGCGAAATACGCGGCCATCGTTATTCCTTCGCCGGCGACTTCGCCAAGGTCGCGAAGGACGGTTCGCT
GATCCTGCTCGGGCGCGGATCGGTTTGCATAAATACTGGCGGCGAGAAGGTCTTCCCCGAGGAAGTCGAGGAAGCGTTGA
AGGCGCATGACAGCGTCTGGGATGCACTGGTGGTCGGCGTGCCCGATGACCGCTTCGGCGAGCGCATCGTGGCGGTGGTT
TCGCAGAGCGAGGGCCACACGATCGACCAGGGCGAAGTGATCGATTTCGCGCGCACCAGGCTGGCCGGATACAAGATGCC
GCGCCAGTTGGTCGTGGTCGACGAAGTGGCCCGCGCCGCGAATGGCAAGGCCGACTATGCCTGGGCTAAGGAAACCGCGC
TGGCGGCGGTCGGCAGCGACGCCTGA

Upstream 100 bases:

>100_bases
GGACTATGCTAGGGCATTGGCCGGAACCTGCAATGAACCGTGACAAGTTTGCCCCCTCGGCACATAGTGCAGCGCAGGGG
ATGCCTGAGGAGAGACAGCC

Downstream 100 bases:

>100_bases
TCAATCCATAAACGGATCGCGCATCAAAATGGTGTCGTCACGCTCGGGGCTGGTCGAGACCAGCGCCACTGGGCATTCGA
TCAGTTCCTGGATGCGCTGG

Product: acyl-CoA synthetase

Products: NA

Alternate protein names: Dihydroxybenzoic acid-activating enzyme [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MIWHFADLFEKIASLVPDNVALIEGDAKRTWREYEDRASRLAAALVEHGIEPDAKVAIYGHNSSAFLEAQFAVFKARAVP
INVNYRYVDDELVYLFDNADVDAVFFDARFAPRLAAIRDKLPKLKLAIQIEDGSGEKLEGAKDLEAVTAGHDPLPRLAYS
EDDHYMVYTGGTTGMPKGVVYRQGDFVKAIAGFILGPDIEPSKEILLGAVKQLAAAGMSPIAFPACPLMHGTGMWLGGFA
AHTAGGAVATLRDEKFDPDRTWEYAAKVGANVIVVVGDAFAKPLLASLRAAAERGEPYDLSRLQAIVSSGAMFSAETKLG
LLEHLDIEIRDAIGSTEGSMGSSITSRAAPPSETAKFELGKDTKVFDENDEEVVPGSDAIGMIANGGFTPIAYYKDPEKS
AKTFREIRGHRYSFAGDFAKVAKDGSLILLGRGSVCINTGGEKVFPEEVEEALKAHDSVWDALVVGVPDDRFGERIVAVV
SQSEGHTIDQGEVIDFARTRLAGYKMPRQLVVVDEVARAANGKADYAWAKETALAAVGSDA

Sequences:

>Translated_541_residues
MIWHFADLFEKIASLVPDNVALIEGDAKRTWREYEDRASRLAAALVEHGIEPDAKVAIYGHNSSAFLEAQFAVFKARAVP
INVNYRYVDDELVYLFDNADVDAVFFDARFAPRLAAIRDKLPKLKLAIQIEDGSGEKLEGAKDLEAVTAGHDPLPRLAYS
EDDHYMVYTGGTTGMPKGVVYRQGDFVKAIAGFILGPDIEPSKEILLGAVKQLAAAGMSPIAFPACPLMHGTGMWLGGFA
AHTAGGAVATLRDEKFDPDRTWEYAAKVGANVIVVVGDAFAKPLLASLRAAAERGEPYDLSRLQAIVSSGAMFSAETKLG
LLEHLDIEIRDAIGSTEGSMGSSITSRAAPPSETAKFELGKDTKVFDENDEEVVPGSDAIGMIANGGFTPIAYYKDPEKS
AKTFREIRGHRYSFAGDFAKVAKDGSLILLGRGSVCINTGGEKVFPEEVEEALKAHDSVWDALVVGVPDDRFGERIVAVV
SQSEGHTIDQGEVIDFARTRLAGYKMPRQLVVVDEVARAANGKADYAWAKETALAAVGSDA
>Mature_541_residues
MIWHFADLFEKIASLVPDNVALIEGDAKRTWREYEDRASRLAAALVEHGIEPDAKVAIYGHNSSAFLEAQFAVFKARAVP
INVNYRYVDDELVYLFDNADVDAVFFDARFAPRLAAIRDKLPKLKLAIQIEDGSGEKLEGAKDLEAVTAGHDPLPRLAYS
EDDHYMVYTGGTTGMPKGVVYRQGDFVKAIAGFILGPDIEPSKEILLGAVKQLAAAGMSPIAFPACPLMHGTGMWLGGFA
AHTAGGAVATLRDEKFDPDRTWEYAAKVGANVIVVVGDAFAKPLLASLRAAAERGEPYDLSRLQAIVSSGAMFSAETKLG
LLEHLDIEIRDAIGSTEGSMGSSITSRAAPPSETAKFELGKDTKVFDENDEEVVPGSDAIGMIANGGFTPIAYYKDPEKS
AKTFREIRGHRYSFAGDFAKVAKDGSLILLGRGSVCINTGGEKVFPEEVEEALKAHDSVWDALVVGVPDDRFGERIVAVV
SQSEGHTIDQGEVIDFARTRLAGYKMPRQLVVVDEVARAANGKADYAWAKETALAAVGSDA

Specific function: Activation of the carboxylate group of 2,3-dihydroxy- benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=548, Percent_Identity=25, Blast_Score=126, Evalue=6e-29,
Organism=Homo sapiens, GI187761343, Length=548, Percent_Identity=25, Blast_Score=126, Evalue=6e-29,
Organism=Homo sapiens, GI156151445, Length=563, Percent_Identity=22.3801065719361, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI42544132, Length=469, Percent_Identity=21.9616204690832, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI38505220, Length=521, Percent_Identity=21.4971209213052, Blast_Score=80, Evalue=7e-15,
Organism=Homo sapiens, GI58082049, Length=553, Percent_Identity=21.3381555153707, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI122937307, Length=239, Percent_Identity=28.0334728033473, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI157311624, Length=236, Percent_Identity=27.1186440677966, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI157311622, Length=236, Percent_Identity=27.1186440677966, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI115511026, Length=529, Percent_Identity=22.3062381852552, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI145693145, Length=531, Percent_Identity=25.0470809792844, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788107, Length=564, Percent_Identity=23.2269503546099, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1786810, Length=527, Percent_Identity=24.2884250474383, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71994694, Length=557, Percent_Identity=24.4165170556553, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71994703, Length=557, Percent_Identity=24.4165170556553, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI71994690, Length=557, Percent_Identity=24.4165170556553, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17560308, Length=537, Percent_Identity=24.5810055865922, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17531443, Length=522, Percent_Identity=22.0306513409962, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17557194, Length=545, Percent_Identity=24.7706422018349, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17559526, Length=528, Percent_Identity=23.2954545454545, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17538037, Length=552, Percent_Identity=23.5507246376812, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17558820, Length=358, Percent_Identity=24.8603351955307, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI32563687, Length=518, Percent_Identity=23.5521235521236, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI115534440, Length=556, Percent_Identity=23.021582733813, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=384, Percent_Identity=24.21875, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6323182, Length=404, Percent_Identity=25, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24581924, Length=559, Percent_Identity=23.4347048300537, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI21355181, Length=495, Percent_Identity=23.6363636363636, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17933690, Length=502, Percent_Identity=23.3067729083665, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24648253, Length=508, Percent_Identity=21.8503937007874, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24648255, Length=508, Percent_Identity=22.244094488189, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356441, Length=387, Percent_Identity=24.2894056847545, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281365686, Length=318, Percent_Identity=24.2138364779874, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: NA

Molecular weight: Translated: 58249; Mature: 58249

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIWHFADLFEKIASLVPDNVALIEGDAKRTWREYEDRASRLAAALVEHGIEPDAKVAIYG
CCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
HNSSAFLEAQFAVFKARAVPINVNYRYVDDELVYLFDNADVDAVFFDARFAPRLAAIRDK
CCCCCEEEEEEEEEEEEEEEEEEEEEEECCEEEEEEECCCCEEEEECCCHHHHHHHHHHC
LPKLKLAIQIEDGSGEKLEGAKDLEAVTAGHDPLPRLAYSEDDHYMVYTGGTTGMPKGVV
CCCEEEEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEE
YRQGDFVKAIAGFILGPDIEPSKEILLGAVKQLAAAGMSPIAFPACPLMHGTGMWLGGFA
EECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCHH
AHTAGGAVATLRDEKFDPDRTWEYAAKVGANVIVVVGDAFAKPLLASLRAAAERGEPYDL
HHCCCCEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCH
SRLQAIVSSGAMFSAETKLGLLEHLDIEIRDAIGSTEGSMGSSITSRAAPPSETAKFELG
HHHHHHHHCCCEEECCHHHHHHHHCCEEEEHHCCCCCCCCCCCHHHCCCCCCCCCEEECC
KDTKVFDENDEEVVPGSDAIGMIANGGFTPIAYYKDPEKSAKTFREIRGHRYSFAGDFAK
CCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCHHHHHHHHHHHCCCCCCCHHHHH
VAKDGSLILLGRGSVCINTGGEKVFPEEVEEALKAHDSVWDALVVGVPDDRFGERIVAVV
HCCCCCEEEEECCCEEEECCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCHHHHHEEEEE
SQSEGHTIDQGEVIDFARTRLAGYKMPRQLVVVDEVARAANGKADYAWAKETALAAVGSD
ECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEHHHHHHHCCCCCCCHHHHHHHHHCCCCC
A
C
>Mature Secondary Structure
MIWHFADLFEKIASLVPDNVALIEGDAKRTWREYEDRASRLAAALVEHGIEPDAKVAIYG
CCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
HNSSAFLEAQFAVFKARAVPINVNYRYVDDELVYLFDNADVDAVFFDARFAPRLAAIRDK
CCCCCEEEEEEEEEEEEEEEEEEEEEEECCEEEEEEECCCCEEEEECCCHHHHHHHHHHC
LPKLKLAIQIEDGSGEKLEGAKDLEAVTAGHDPLPRLAYSEDDHYMVYTGGTTGMPKGVV
CCCEEEEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEE
YRQGDFVKAIAGFILGPDIEPSKEILLGAVKQLAAAGMSPIAFPACPLMHGTGMWLGGFA
EECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCHH
AHTAGGAVATLRDEKFDPDRTWEYAAKVGANVIVVVGDAFAKPLLASLRAAAERGEPYDL
HHCCCCEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCH
SRLQAIVSSGAMFSAETKLGLLEHLDIEIRDAIGSTEGSMGSSITSRAAPPSETAKFELG
HHHHHHHHCCCEEECCHHHHHHHHCCEEEEHHCCCCCCCCCCCHHHCCCCCCCCCEEECC
KDTKVFDENDEEVVPGSDAIGMIANGGFTPIAYYKDPEKSAKTFREIRGHRYSFAGDFAK
CCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCHHHHHHHHHHHCCCCCCCHHHHH
VAKDGSLILLGRGSVCINTGGEKVFPEEVEEALKAHDSVWDALVVGVPDDRFGERIVAVV
HCCCCCEEEEECCCEEEECCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCHHHHHEEEEE
SQSEGHTIDQGEVIDFARTRLAGYKMPRQLVVVDEVARAANGKADYAWAKETALAAVGSD
ECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEHHHHHHHCCCCCCCHHHHHHHHHCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8921902; 9384377; 8224884; 12221282 [H]