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The map label for this gene is sir2 [H]

Identifier: 85373203

GI number: 85373203

Start: 421490

End: 423124

Strand: Reverse

Name: sir2 [H]

Synonym: ELI_01880

Alternate gene names: 85373203

Gene position: 423124-421490 (Counterclockwise)

Preceding gene: 85373204

Following gene: 85373202

Centisome position: 13.86

GC content: 60.8

Gene sequence:

>1635_bases
ATGTATCAATACGACCACTACGATCAGGCCATGGTCGACGCCCGCGTCGAGGAATTCCGCGACCAGACCCGGCGCCGGCT
CGAAGGCAAGATCACCGAGGACCAGTTCAAGCCGCTGCGGCTGATGAACGGGCTCTACCTGCAATTGCACGCCTACATGC
TGCGCGTCGCCATTCCCTACGGCACGCTCAATAGCGCGCAGATGCATGCGCTGGCCGATATCGCCGACAAATACGACCGC
GGTTACGGCCACTTCACCACGCGGCAGAACATCCAGTATAACTGGATCAAGCTGGAAGACGCGCCCGACCTGCTGGCCGA
TCTCGCCAAGGTCGAAATGCATGCAATCCAGACCAGCGGGAACTGCATCCGCAATATCAGCTCCGATCATTTTGCCGGGG
CCGCGGCGGATGAACTCATCGATCCAAGGCCTTACGCCGAATTGCTCAGGCAATGGTCGAGCTTCCACCCGGAATTCAGC
TACCTGCCGCGCAAATTCAAGATTGCCGTGATCGCCAGCGAGACCGACCGCGCGGCGATGAAGCTGCACGATATCGGCAT
CCAGATCGTCAAGAACGATGCGGGCGAGATCGGCGCGGCCTTTTATGTCGGCGGCGGCATGGGGCGCACGCCCATGATCG
CCCCGTTGATCCGCGACTTCGTGCCGCTCGATCACCTTGTGACCTATGCCGAGGCATGCCTGCGCGTCTACAACCGCTAC
GGACGGCGCGACAACAAGTACAAGGCTCGGATCAAGATCCTCGTCCACGAACTGGGCGCGGAGGAATATACCCGGCAGGT
CGAGGAGGAATTCCAGCACCTGCTCGACCAGGGTGTCGAGCCGCCACGCGCGGAACTCGAGCGGATCATTCCTTACTTTG
CCGACCCGGCTTTTGATGACGCGAGCAAGGACGCAATCGACCGCAGCAATCCCGACTTCGCCGACTGGGTCGATGCCAAC
ACCCACCCGCACAAGCGCGACGGCTACGTCAGCGCGGTGATCAGCCTGAAGCCGGTCGGCGGCATTCCGGGCGATGCCAG
CGCGCACCAGATGCATGTCATGGCCGACCTCGCGAGAGAGTATTCGTTCGACGAGTGCCGCGTCATGCACACGCAGAACA
TCGTATTACCGCATGTCCGCAAGAGTGAACTCTATGCACTGTGGCAAAAGCTGGAATCCGAGGGACTTGGGGCGCCCAAC
CTCGACACGATCGAAGATATCATCGCCTGCCCCGGCCTCGATTACTGCAGCCTTGCCAATGCCCGCTCGATCCCCGTCGC
GCAGAAGATTTCCGAACGCTTCGCCGCCAAAGGCCGGACAAAGGAACTGGGCGAACTGAAGCTGAAGATTTCCGGCTGCA
TCAATGCCTGCGGGCACCACCATGCGGGCCATATCGGCATCCTCGGCGTCGACAAGAAAGGCACCGAAAATTACCAGCTG
CTGCTCGGCGGTAGCGAAGCGCAGGACACTGCGCTCGCCAAGATCACCGGTCCCGGCTTCGACGAGGAAGGCATCGTCGA
CGCGGTCGAGACCGCAACCGATGTGTACCTTTCCAACCGCGAGAACGGAGAGCGCTTCCTCGATACCTATCGCCGCATTG
GAATGGCCCCGTTCAAGGAGGCGTTGTATGACTGA

Upstream 100 bases:

>100_bases
GCCCACATCAAGGACCGCGTTCGCGCGCTCGGCCCCACCGTGCGCCCCGACCTGACGCTCAAACCCAAAGACCCCGAAGC
CATGGACTGGGTGATCTGAC

Downstream 100 bases:

>100_bases
GTCCCCCAAGCCTGCCAGCGACACGGTGTCCGGTACCAGCCCCGACGAAGTGCAGTTCCGCTTCCGCGACGACGAGGTTG
CCGATCACGCGCAAGTGACG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MYQYDHYDQAMVDARVEEFRDQTRRRLEGKITEDQFKPLRLMNGLYLQLHAYMLRVAIPYGTLNSAQMHALADIADKYDR
GYGHFTTRQNIQYNWIKLEDAPDLLADLAKVEMHAIQTSGNCIRNISSDHFAGAAADELIDPRPYAELLRQWSSFHPEFS
YLPRKFKIAVIASETDRAAMKLHDIGIQIVKNDAGEIGAAFYVGGGMGRTPMIAPLIRDFVPLDHLVTYAEACLRVYNRY
GRRDNKYKARIKILVHELGAEEYTRQVEEEFQHLLDQGVEPPRAELERIIPYFADPAFDDASKDAIDRSNPDFADWVDAN
THPHKRDGYVSAVISLKPVGGIPGDASAHQMHVMADLAREYSFDECRVMHTQNIVLPHVRKSELYALWQKLESEGLGAPN
LDTIEDIIACPGLDYCSLANARSIPVAQKISERFAAKGRTKELGELKLKISGCINACGHHHAGHIGILGVDKKGTENYQL
LLGGSEAQDTALAKITGPGFDEEGIVDAVETATDVYLSNRENGERFLDTYRRIGMAPFKEALYD

Sequences:

>Translated_544_residues
MYQYDHYDQAMVDARVEEFRDQTRRRLEGKITEDQFKPLRLMNGLYLQLHAYMLRVAIPYGTLNSAQMHALADIADKYDR
GYGHFTTRQNIQYNWIKLEDAPDLLADLAKVEMHAIQTSGNCIRNISSDHFAGAAADELIDPRPYAELLRQWSSFHPEFS
YLPRKFKIAVIASETDRAAMKLHDIGIQIVKNDAGEIGAAFYVGGGMGRTPMIAPLIRDFVPLDHLVTYAEACLRVYNRY
GRRDNKYKARIKILVHELGAEEYTRQVEEEFQHLLDQGVEPPRAELERIIPYFADPAFDDASKDAIDRSNPDFADWVDAN
THPHKRDGYVSAVISLKPVGGIPGDASAHQMHVMADLAREYSFDECRVMHTQNIVLPHVRKSELYALWQKLESEGLGAPN
LDTIEDIIACPGLDYCSLANARSIPVAQKISERFAAKGRTKELGELKLKISGCINACGHHHAGHIGILGVDKKGTENYQL
LLGGSEAQDTALAKITGPGFDEEGIVDAVETATDVYLSNRENGERFLDTYRRIGMAPFKEALYD
>Mature_544_residues
MYQYDHYDQAMVDARVEEFRDQTRRRLEGKITEDQFKPLRLMNGLYLQLHAYMLRVAIPYGTLNSAQMHALADIADKYDR
GYGHFTTRQNIQYNWIKLEDAPDLLADLAKVEMHAIQTSGNCIRNISSDHFAGAAADELIDPRPYAELLRQWSSFHPEFS
YLPRKFKIAVIASETDRAAMKLHDIGIQIVKNDAGEIGAAFYVGGGMGRTPMIAPLIRDFVPLDHLVTYAEACLRVYNRY
GRRDNKYKARIKILVHELGAEEYTRQVEEEFQHLLDQGVEPPRAELERIIPYFADPAFDDASKDAIDRSNPDFADWVDAN
THPHKRDGYVSAVISLKPVGGIPGDASAHQMHVMADLAREYSFDECRVMHTQNIVLPHVRKSELYALWQKLESEGLGAPN
LDTIEDIIACPGLDYCSLANARSIPVAQKISERFAAKGRTKELGELKLKISGCINACGHHHAGHIGILGVDKKGTENYQL
LLGGSEAQDTALAKITGPGFDEEGIVDAVETATDVYLSNRENGERFLDTYRRIGMAPFKEALYD

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322597, Length=564, Percent_Identity=22.3404255319149, Blast_Score=88, Evalue=4e-18,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 61202; Mature: 61202

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQYDHYDQAMVDARVEEFRDQTRRRLEGKITEDQFKPLRLMNGLYLQLHAYMLRVAIPY
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GTLNSAQMHALADIADKYDRGYGHFTTRQNIQYNWIKLEDAPDLLADLAKVEMHAIQTSG
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCC
NCIRNISSDHFAGAAADELIDPRPYAELLRQWSSFHPEFSYLPRKFKIAVIASETDRAAM
HHHHCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCHHHCCCEEEEEEEECCCCHHHH
KLHDIGIQIVKNDAGEIGAAFYVGGGMGRTPMIAPLIRDFVPLDHLVTYAEACLRVYNRY
HHHHCCEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
GRRDNKYKARIKILVHELGAEEYTRQVEEEFQHLLDQGVEPPRAELERIIPYFADPAFDD
CCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCC
ASKDAIDRSNPDFADWVDANTHPHKRDGYVSAVISLKPVGGIPGDASAHQMHVMADLARE
CCHHHCCCCCCCHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHH
YSFDECRVMHTQNIVLPHVRKSELYALWQKLESEGLGAPNLDTIEDIIACPGLDYCSLAN
CCCCHHEEEECCCEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHCCC
ARSIPVAQKISERFAAKGRTKELGELKLKISGCINACGHHHAGHIGILGVDKKGTENYQL
CCCCCHHHHHHHHHHHCCCCCCHHHEEEHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEE
LLGGSEAQDTALAKITGPGFDEEGIVDAVETATDVYLSNRENGERFLDTYRRIGMAPFKE
EECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHH
ALYD
HHCC
>Mature Secondary Structure
MYQYDHYDQAMVDARVEEFRDQTRRRLEGKITEDQFKPLRLMNGLYLQLHAYMLRVAIPY
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GTLNSAQMHALADIADKYDRGYGHFTTRQNIQYNWIKLEDAPDLLADLAKVEMHAIQTSG
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCC
NCIRNISSDHFAGAAADELIDPRPYAELLRQWSSFHPEFSYLPRKFKIAVIASETDRAAM
HHHHCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCHHHCCCEEEEEEEECCCCHHHH
KLHDIGIQIVKNDAGEIGAAFYVGGGMGRTPMIAPLIRDFVPLDHLVTYAEACLRVYNRY
HHHHCCEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
GRRDNKYKARIKILVHELGAEEYTRQVEEEFQHLLDQGVEPPRAELERIIPYFADPAFDD
CCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCC
ASKDAIDRSNPDFADWVDANTHPHKRDGYVSAVISLKPVGGIPGDASAHQMHVMADLARE
CCHHHCCCCCCCHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHH
YSFDECRVMHTQNIVLPHVRKSELYALWQKLESEGLGAPNLDTIEDIIACPGLDYCSLAN
CCCCHHEEEECCCEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHCCC
ARSIPVAQKISERFAAKGRTKELGELKLKISGCINACGHHHAGHIGILGVDKKGTENYQL
CCCCCHHHHHHHHHHHCCCCCCHHHEEEHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEE
LLGGSEAQDTALAKITGPGFDEEGIVDAVETATDVYLSNRENGERFLDTYRRIGMAPFKE
EECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHH
ALYD
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA