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The map label for this gene is cysH [H]

Identifier: 85373201

GI number: 85373201

Start: 420243

End: 421028

Strand: Reverse

Name: cysH [H]

Synonym: ELI_01870

Alternate gene names: 85373201

Gene position: 421028-420243 (Counterclockwise)

Preceding gene: 85373202

Following gene: 85373171

Centisome position: 13.79

GC content: 63.61

Gene sequence:

>786_bases
ATGAATGAGCTTCGCGCGATCGACCGGCTCGACACCGGCCCCCGCTTCACCGACCACGACGCGGTGCGCCTCAACCGCAT
GTTTCGCGGCAGCAGCACCGAAGAAATGCTCGAATCCGTCATCAGGGACGGGCTGGCAGGCGACGTCACAACCGTTTCCA
GCTTCGGCGCGGAAAGCGCTGTTCTGCTGCATCTTCTGGCGCAGGTCGACCCGGGCATTCCGGTACTGTTCCTAGAAACG
GGCAAGCACTTTCCCGAAACGCTCGCCTATCGCGACGACCTGGTCGAGCGCATCGGCCTGACCAATCTCATCAACCTCTA
TCCCGACCTGAGCGAACTGGAGACTAAGGACGAGACCGGCCTGCGCTGGTCCTACGATCCCGACGGCTGCTGCGAGATCC
GCAAGGTCAAGCCGCTGGCCAGGGCGCTTTCCGAATACGATGCCAGCTTTACCGGTCGCAAGGCGTTCCAGAACAGCGAA
CGGGCCAACCTGCCGCGCTTCGAAATCGACACTTCCGACGCGCAGGGCCGCCTCAAGATCAACCCGCTGATCGATTGGGA
TGCGAGCCAGATCGAAGCCTATTTTATCCAGCACGACCTGCCGCGCCATCCGTTGGTCGCGCAGGGCTATCCCTCGATCG
GCTGCTCCCCCTGCACCCACAAGGTCGCACCGGGCGAAGACCCCCGCTCGGGCCGCTGGAGGGGCTGGGACAAAACCGAA
TGCGGCATCCACATGCCGGGCAGCGACGACGGCGATTTGCCCCCCGGCTACGATCCGGCGTTCTGA

Upstream 100 bases:

>100_bases
AAGGCGATCGACGAGGACGATGCAAAGGCCGCCTTCGAACGCTGGCCGCACGTCTATCAATCCGCCGCCGATGCCCGCCG
CACGATCCCCGAGTTGCGCC

Downstream 100 bases:

>100_bases
CGCCTTACAACCACTTGTTGTCCCGGTACCATTTGGCGGAGGCCTTCAGACCCTCGCGGGTCGGAATCTTGGGTTGCCAC
AGCTCCCGGGGCGGTCTGGC

Product: phosphoadenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MNELRAIDRLDTGPRFTDHDAVRLNRMFRGSSTEEMLESVIRDGLAGDVTTVSSFGAESAVLLHLLAQVDPGIPVLFLET
GKHFPETLAYRDDLVERIGLTNLINLYPDLSELETKDETGLRWSYDPDGCCEIRKVKPLARALSEYDASFTGRKAFQNSE
RANLPRFEIDTSDAQGRLKINPLIDWDASQIEAYFIQHDLPRHPLVAQGYPSIGCSPCTHKVAPGEDPRSGRWRGWDKTE
CGIHMPGSDDGDLPPGYDPAF

Sequences:

>Translated_261_residues
MNELRAIDRLDTGPRFTDHDAVRLNRMFRGSSTEEMLESVIRDGLAGDVTTVSSFGAESAVLLHLLAQVDPGIPVLFLET
GKHFPETLAYRDDLVERIGLTNLINLYPDLSELETKDETGLRWSYDPDGCCEIRKVKPLARALSEYDASFTGRKAFQNSE
RANLPRFEIDTSDAQGRLKINPLIDWDASQIEAYFIQHDLPRHPLVAQGYPSIGCSPCTHKVAPGEDPRSGRWRGWDKTE
CGIHMPGSDDGDLPPGYDPAF
>Mature_261_residues
MNELRAIDRLDTGPRFTDHDAVRLNRMFRGSSTEEMLESVIRDGLAGDVTTVSSFGAESAVLLHLLAQVDPGIPVLFLET
GKHFPETLAYRDDLVERIGLTNLINLYPDLSELETKDETGLRWSYDPDGCCEIRKVKPLARALSEYDASFTGRKAFQNSE
RANLPRFEIDTSDAQGRLKINPLIDWDASQIEAYFIQHDLPRHPLVAQGYPSIGCSPCTHKVAPGEDPRSGRWRGWDKTE
CGIHMPGSDDGDLPPGYDPAF

Specific function: Reduction of activated sulfate into sulfite [H]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=207, Percent_Identity=38.1642512077295, Blast_Score=122, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6325425, Length=204, Percent_Identity=36.7647058823529, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =1.8.4.8 [H]

Molecular weight: Translated: 29131; Mature: 29131

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELRAIDRLDTGPRFTDHDAVRLNRMFRGSSTEEMLESVIRDGLAGDVTTVSSFGAESA
CCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHCCCHHH
VLLHLLAQVDPGIPVLFLETGKHFPETLAYRDDLVERIGLTNLINLYPDLSELETKDETG
HHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCC
LRWSYDPDGCCEIRKVKPLARALSEYDASFTGRKAFQNSERANLPRFEIDTSDAQGRLKI
CEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEECCCCCCCEEEE
NPLIDWDASQIEAYFIQHDLPRHPLVAQGYPSIGCSPCTHKVAPGEDPRSGRWRGWDKTE
CCEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
CGIHMPGSDDGDLPPGYDPAF
CCEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNELRAIDRLDTGPRFTDHDAVRLNRMFRGSSTEEMLESVIRDGLAGDVTTVSSFGAESA
CCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHCCCHHH
VLLHLLAQVDPGIPVLFLETGKHFPETLAYRDDLVERIGLTNLINLYPDLSELETKDETG
HHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCC
LRWSYDPDGCCEIRKVKPLARALSEYDASFTGRKAFQNSERANLPRFEIDTSDAQGRLKI
CEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEECCCCCCCEEEE
NPLIDWDASQIEAYFIQHDLPRHPLVAQGYPSIGCSPCTHKVAPGEDPRSGRWRGWDKTE
CCEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
CGIHMPGSDDGDLPPGYDPAF
CCEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA