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The map label for this gene is yuxL [H]

Identifier: 85373170

GI number: 85373170

Start: 387041

End: 389005

Strand: Reverse

Name: yuxL [H]

Synonym: ELI_01715

Alternate gene names: 85373170

Gene position: 389005-387041 (Counterclockwise)

Preceding gene: 85373171

Following gene: 85373169

Centisome position: 12.74

GC content: 62.9

Gene sequence:

>1965_bases
ATGACCCCCAACATCGACGCTTTTCCGCTCATCCGGCGCGAACATCTGTTCGGCAACCCGACCCGCGCTGCCGGGCAGAT
CAGCCCCGACGGCCAATGGCTGAGCTGGCTCGCCCCGCGCGACGGCGTGCTCAATATCTGGATGGCGCCGAGCGAGGATC
CCGAGGCGGCCAAGCCCATGTCCGCTTCCACCGACCGTCCGATCCGCGAATATATGTGGGCGCCCGACAGCCGCAGCCTG
CTCTACATCCAAGACAAGGGCGGCGACGAGAATTTCCTGCTCTACGGGATCGATGTGGAGGCCGGCAAGGAGACCTCACT
AACCCCGTTCGATGATACGCGCGTCATGCTGGTCGGCGGTTCCGACACATACAAGGACCGCATCCTGATCGGGCTCAACA
ACCGCGATGCGCGCTTCCACGATGTCCACCTGCTCGACCTCAACACCGGCGAACTGACGCTGGTGTTGCAGAACGACGCC
TATGCCGGTTTCATCGCCGATGACGACCTGCACCTGCGGCTCGCGGTGTTGCCGAATGCCGAAGGCGGGACGGACTATTT
CAAGGTCGAAGACAATGTCGTCGCCGATGCGCCGTTCAAGTCAACCGGGATTGACGATGCGCTGACCACTTCGCCCGGCG
GTTTCACGCGCGACGGCAAGACGATGTACTGGCTCGAAAGCGAAGGCCGCAACACCGCTGCACTGCTGGCGATGGACTGG
GTAAGCGGCACAACGACGTTGATCGCGCAAGACGACCGCGCCGATATCGGGGGCACATTGCGGCACAAGGATACCGGCGA
GGTGTTGGCCTACTCGGTCAATTACCTTAAGCGCGAATGGACTGCGCTGGACGACGATATCGCCGGAGCCTTCGAGTTCC
TCGATAGCCGGTTGGAGGGCGAATACGGCATCAACAGTCGCACCGACGATGATCGCAAGTGGATCGTCGGCAATGACCCG
CTGGTTAAACCCGCGGCGAGCTATCTCTACGATCGCGATGCGGAAACCTTGACCAAGCTCTACACCACCCGGCCCGAGCT
GGAAGGCGCACCGCTTCAGCCGATGCATACACTGGAGCTGACCAGCCGCGACGGGCTCACCCTGCCCTCCTACCTCACCC
TGCCGCCGGGAAGCGACGAAGATGGCGACGGGGTGCCGGATGCGCCGGTGCCGATGGTGCTGTTCGTTCATGGCGGGCCG
TGGGCGCGCGACGGATATGGCTTCAACGGCCACCACCAGTGGCTCGCCAATCGCGGCTATGCGGTGCTCAGCGTCAACTT
CCGCGGCTCGACCGGCTTCGGCAAGGATTTCATCAACGCGGCAAATCTCGAATGGGGCCGCAAGATGCATGACGATTTGA
TCGACGCGGTCGAGTGGGCAGTCGAGGCTGGCATCGCTCCGCGTGACAAGATCGCGATCATGGGTGGCTCCTATGGCGGC
TACGCCACGCTGGCGGGCCTGACTTTCACGCCCGAAGTATTCGCGTGCGGCGTCGATATCGTCGGCCCGTCCAACCTCGA
AACGTTGCTGGCATCGATCCCGCCCTATTGGGAGCCGATGGTCGCGCAATTCCACACCCGCATGGGCAATCCCAACACCG
AACAAGGCCTCGCGCTGATCAAGGAGCGCAGCCCGCTCTACAAGGCTGGCGAGATCGTCCGGCCGCTGTTGATAGCGCAG
GGAGCCAACGACCCGCGCGTCAAGCAGCCGGAAAGCGACCAGATCGTGGATGCGATGAAGGATGCAGGCATCCCGGTCAC
GTATTTGCTCTATCCCGACGAAGGCCACGGCTTTGCCAAGCCGGCCAACAATATCGCCTTCAGCGCGGTGGCGGAGAATT
TCCTCGCCACATATCTCGGCGGTCGGGCAGAACCTATCGGCGATACCTTGCGCAGTTCGACGGCGGAAATCCCGGCCGGT
GCCGAGTTCGTCGAAGGCTTGGCAGAGGCGCTGGAGAGCGCGTAG

Upstream 100 bases:

>100_bases
TCGCGGGGCGGTCGCAATCTTCGCGTGGCATACCTACAATATGGAAGCGATCTGATCCGCACCGCGGATCGGCCTTCACT
GTCACAACAACGGATCCTGA

Downstream 100 bases:

>100_bases
GATACCAGCACAGGGATTACGGGGAGAGGACGGATGAGCACCCGCAAGAGCATATTCATCACCGGCGGCGGTTCCGGCAT
CGGGCGCGCCATCGCGCTCA

Product: dipeptidyl aminopeptidase

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 654; Mature: 653

Protein sequence:

>654_residues
MTPNIDAFPLIRREHLFGNPTRAAGQISPDGQWLSWLAPRDGVLNIWMAPSEDPEAAKPMSASTDRPIREYMWAPDSRSL
LYIQDKGGDENFLLYGIDVEAGKETSLTPFDDTRVMLVGGSDTYKDRILIGLNNRDARFHDVHLLDLNTGELTLVLQNDA
YAGFIADDDLHLRLAVLPNAEGGTDYFKVEDNVVADAPFKSTGIDDALTTSPGGFTRDGKTMYWLESEGRNTAALLAMDW
VSGTTTLIAQDDRADIGGTLRHKDTGEVLAYSVNYLKREWTALDDDIAGAFEFLDSRLEGEYGINSRTDDDRKWIVGNDP
LVKPAASYLYDRDAETLTKLYTTRPELEGAPLQPMHTLELTSRDGLTLPSYLTLPPGSDEDGDGVPDAPVPMVLFVHGGP
WARDGYGFNGHHQWLANRGYAVLSVNFRGSTGFGKDFINAANLEWGRKMHDDLIDAVEWAVEAGIAPRDKIAIMGGSYGG
YATLAGLTFTPEVFACGVDIVGPSNLETLLASIPPYWEPMVAQFHTRMGNPNTEQGLALIKERSPLYKAGEIVRPLLIAQ
GANDPRVKQPESDQIVDAMKDAGIPVTYLLYPDEGHGFAKPANNIAFSAVAENFLATYLGGRAEPIGDTLRSSTAEIPAG
AEFVEGLAEALESA

Sequences:

>Translated_654_residues
MTPNIDAFPLIRREHLFGNPTRAAGQISPDGQWLSWLAPRDGVLNIWMAPSEDPEAAKPMSASTDRPIREYMWAPDSRSL
LYIQDKGGDENFLLYGIDVEAGKETSLTPFDDTRVMLVGGSDTYKDRILIGLNNRDARFHDVHLLDLNTGELTLVLQNDA
YAGFIADDDLHLRLAVLPNAEGGTDYFKVEDNVVADAPFKSTGIDDALTTSPGGFTRDGKTMYWLESEGRNTAALLAMDW
VSGTTTLIAQDDRADIGGTLRHKDTGEVLAYSVNYLKREWTALDDDIAGAFEFLDSRLEGEYGINSRTDDDRKWIVGNDP
LVKPAASYLYDRDAETLTKLYTTRPELEGAPLQPMHTLELTSRDGLTLPSYLTLPPGSDEDGDGVPDAPVPMVLFVHGGP
WARDGYGFNGHHQWLANRGYAVLSVNFRGSTGFGKDFINAANLEWGRKMHDDLIDAVEWAVEAGIAPRDKIAIMGGSYGG
YATLAGLTFTPEVFACGVDIVGPSNLETLLASIPPYWEPMVAQFHTRMGNPNTEQGLALIKERSPLYKAGEIVRPLLIAQ
GANDPRVKQPESDQIVDAMKDAGIPVTYLLYPDEGHGFAKPANNIAFSAVAENFLATYLGGRAEPIGDTLRSSTAEIPAG
AEFVEGLAEALESA
>Mature_653_residues
TPNIDAFPLIRREHLFGNPTRAAGQISPDGQWLSWLAPRDGVLNIWMAPSEDPEAAKPMSASTDRPIREYMWAPDSRSLL
YIQDKGGDENFLLYGIDVEAGKETSLTPFDDTRVMLVGGSDTYKDRILIGLNNRDARFHDVHLLDLNTGELTLVLQNDAY
AGFIADDDLHLRLAVLPNAEGGTDYFKVEDNVVADAPFKSTGIDDALTTSPGGFTRDGKTMYWLESEGRNTAALLAMDWV
SGTTTLIAQDDRADIGGTLRHKDTGEVLAYSVNYLKREWTALDDDIAGAFEFLDSRLEGEYGINSRTDDDRKWIVGNDPL
VKPAASYLYDRDAETLTKLYTTRPELEGAPLQPMHTLELTSRDGLTLPSYLTLPPGSDEDGDGVPDAPVPMVLFVHGGPW
ARDGYGFNGHHQWLANRGYAVLSVNFRGSTGFGKDFINAANLEWGRKMHDDLIDAVEWAVEAGIAPRDKIAIMGGSYGGY
ATLAGLTFTPEVFACGVDIVGPSNLETLLASIPPYWEPMVAQFHTRMGNPNTEQGLALIKERSPLYKAGEIVRPLLIAQG
ANDPRVKQPESDQIVDAMKDAGIPVTYLLYPDEGHGFAKPANNIAFSAVAENFLATYLGGRAEPIGDTLRSSTAEIPAGA
EFVEGLAEALESA

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1506

COG function: function code E; Dipeptidyl aminopeptidases/acylaminoacyl-peptidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI23510451, Length=250, Percent_Identity=30.8, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI16933540, Length=298, Percent_Identity=25.8389261744966, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI194394146, Length=246, Percent_Identity=30.0813008130081, Blast_Score=73, Evalue=1e-12,
Organism=Homo sapiens, GI18765694, Length=232, Percent_Identity=27.1551724137931, Blast_Score=71, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI25144537, Length=681, Percent_Identity=37.151248164464, Blast_Score=429, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI25144540, Length=443, Percent_Identity=42.6636568848758, Blast_Score=340, Evalue=1e-93,
Organism=Caenorhabditis elegans, GI25144543, Length=544, Percent_Identity=36.0294117647059, Blast_Score=334, Evalue=8e-92,
Organism=Caenorhabditis elegans, GI17552908, Length=212, Percent_Identity=29.7169811320755, Blast_Score=100, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI25149159, Length=246, Percent_Identity=30.8943089430894, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI45550825, Length=241, Percent_Identity=27.3858921161826, Blast_Score=76, Evalue=9e-14,
Organism=Drosophila melanogaster, GI45553511, Length=241, Percent_Identity=27.3858921161826, Blast_Score=76, Evalue=9e-14,
Organism=Drosophila melanogaster, GI45551969, Length=241, Percent_Identity=27.3858921161826, Blast_Score=76, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011042
- InterPro:   IPR011659
- InterPro:   IPR001375 [H]

Pfam domain/function: PF07676 PD40; PF00326 Peptidase_S9 [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 71351; Mature: 71220

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPNIDAFPLIRREHLFGNPTRAAGQISPDGQWLSWLAPRDGVLNIWMAPSEDPEAAKPM
CCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCC
SASTDRPIREYMWAPDSRSLLYIQDKGGDENFLLYGIDVEAGKETSLTPFDDTRVMLVGG
CCCCCCCHHHHHCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEECC
SDTYKDRILIGLNNRDARFHDVHLLDLNTGELTLVLQNDAYAGFIADDDLHLRLAVLPNA
CCCCCCEEEEEECCCCCEEEEEEEEEECCCEEEEEEECCCEEEEEECCCCEEEEEEECCC
EGGTDYFKVEDNVVADAPFKSTGIDDALTTSPGGFTRDGKTMYWLESEGRNTAALLAMDW
CCCCCEEEECCCEEECCCCCCCCCCCHHCCCCCCCCCCCCEEEEEECCCCCEEEEEEEEC
VSGTTTLIAQDDRADIGGTLRHKDTGEVLAYSVNYLKREWTALDDDIAGAFEFLDSRLEG
CCCCEEEEEECCCCCCCCEEECCCCCCEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHCCC
EYGINSRTDDDRKWIVGNDPLVKPAASYLYDRDAETLTKLYTTRPELEGAPLQPMHTLEL
CCCCCCCCCCCCEEEECCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEEEE
TSRDGLTLPSYLTLPPGSDEDGDGVPDAPVPMVLFVHGGPWARDGYGFNGHHQWLANRGY
ECCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHCCCE
AVLSVNFRGSTGFGKDFINAANLEWGRKMHDDLIDAVEWAVEAGIAPRDKIAIMGGSYGG
EEEEEEECCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
YATLAGLTFTPEVFACGVDIVGPSNLETLLASIPPYWEPMVAQFHTRMGNPNTEQGLALI
EEEEECCEECCHHHEECEEEECCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHH
KERSPLYKAGEIVRPLLIAQGANDPRVKQPESDQIVDAMKDAGIPVTYLLYPDEGHGFAK
HCCCCCHHHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCC
PANNIAFSAVAENFLATYLGGRAEPIGDTLRSSTAEIPAGAEFVEGLAEALESA
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TPNIDAFPLIRREHLFGNPTRAAGQISPDGQWLSWLAPRDGVLNIWMAPSEDPEAAKPM
CCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCC
SASTDRPIREYMWAPDSRSLLYIQDKGGDENFLLYGIDVEAGKETSLTPFDDTRVMLVGG
CCCCCCCHHHHHCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEECC
SDTYKDRILIGLNNRDARFHDVHLLDLNTGELTLVLQNDAYAGFIADDDLHLRLAVLPNA
CCCCCCEEEEEECCCCCEEEEEEEEEECCCEEEEEEECCCEEEEEECCCCEEEEEEECCC
EGGTDYFKVEDNVVADAPFKSTGIDDALTTSPGGFTRDGKTMYWLESEGRNTAALLAMDW
CCCCCEEEECCCEEECCCCCCCCCCCHHCCCCCCCCCCCCEEEEEECCCCCEEEEEEEEC
VSGTTTLIAQDDRADIGGTLRHKDTGEVLAYSVNYLKREWTALDDDIAGAFEFLDSRLEG
CCCCEEEEEECCCCCCCCEEECCCCCCEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHCCC
EYGINSRTDDDRKWIVGNDPLVKPAASYLYDRDAETLTKLYTTRPELEGAPLQPMHTLEL
CCCCCCCCCCCCEEEECCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEEEE
TSRDGLTLPSYLTLPPGSDEDGDGVPDAPVPMVLFVHGGPWARDGYGFNGHHQWLANRGY
ECCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHCCCE
AVLSVNFRGSTGFGKDFINAANLEWGRKMHDDLIDAVEWAVEAGIAPRDKIAIMGGSYGG
EEEEEEECCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
YATLAGLTFTPEVFACGVDIVGPSNLETLLASIPPYWEPMVAQFHTRMGNPNTEQGLALI
EEEEECCEECCHHHEECEEEECCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHH
KERSPLYKAGEIVRPLLIAQGANDPRVKQPESDQIVDAMKDAGIPVTYLLYPDEGHGFAK
HCCCCCHHHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCC
PANNIAFSAVAENFLATYLGGRAEPIGDTLRSSTAEIPAGAEFVEGLAEALESA
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.23 {tosyl-Arg} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 80 {acetyl-tyrosine} 0.47 {tosyl-Lys-methyl} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 3098560 [H]