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The map label for this gene is glnA [H]

Identifier: 85373159

GI number: 85373159

Start: 377926

End: 379335

Strand: Reverse

Name: glnA [H]

Synonym: ELI_01660

Alternate gene names: 85373159

Gene position: 379335-377926 (Counterclockwise)

Preceding gene: 85373160

Following gene: 85373158

Centisome position: 12.43

GC content: 60.99

Gene sequence:

>1410_bases
ATGTCCAGCGCAAAAGACATCATCAAGAAAATCAAGGAACACGAGATCGAGTGGGTCGATCTGCGGTTCACCGATCCGAA
GGGCAAGTGGCAGCACCTTTCGATGGTCGCCAGCGTGATGGACGAAGATGCGCTCGAAGACGGGCTGATGTTCGACGGAT
CGTCGATCGCGGGCTGGAAGGCCATCAACGAATCCGACATGATCCTGAAGCCGGACCTGTCCGAAACCTGGCTCGATCCG
TTCAGCGCCACGCCGATGCTGATCGTCAATTGCGACATCGTCGAGCCCTCGACCGGCGACTGGTATTCCCGCGATCCCCG
TTCCACCGCCAAGCGTGCCGAAGCCTATCTGAAGAGCACCGGTATCGGCGACACGATCTATGTCGGTCCGGAAGCCGAAT
TCTTCATGTTCGACGATGTCCGCTTCGAAGACGGCTACGCGGGGTCGGGCTTCGCCATCGACGACATCGAATTGCCGACC
AACATGGGCAAGGAATACGAGGGCGGCAACATGGGCCACCGGCCGCGCGCCAAGGGCGGCTATTTCCCCGTCGCTCCGGT
GGACAGCGCTATGGACATTCGCGCCGAAATGGTCGCGACAATGCTCGAACTGGGCTTGCCCTGCGACAAGCACCACCATG
AGGTCGCCGCCGCCCAGCACGAACTCGGCCTGACCTTCGGCACGCTGGTCCAGACCGCCGACCGCATGCAGATCTACAAG
TATGTCGTACACCAGGTCGCCCATGCCTATGGCAAGACCGCGACCTTCATGCCCAAGCCGATCAAGGAAGATAACGGTTC
CGGCATGCACACGCACATGTCGATCTGGGAAGGCGACAAGCCGACATTCGCCGGCAACGAATATGCCGGGCTGAGCGAGA
ACTGCCTCTATTACATCGGCGGTGTCATCAAGCACGCCAAGGCGCTGAACGCTTTCACCAATCCGACCACCAACAGCTAC
AAGCGGCTAGTTCCGGGCTTCGAAGCACCGGTGCTGCTCGCCTATTCGGCCCGCAACCGCTCGGCATCGTGCCGCATCCC
GTACGGCGCGGGCGACAAGGCGAAGCGCGTCGAGTTCCGCTTCCCCGATGCGATGGCCAATCCCTACCTCGCCTATTCGG
CGCTGCTGATGGCGGGCCTCGACGGGATCACCAACAAGATCCATCCGGGCGATGCCATGGACAAGAACCTCTACGACCTG
CCGCCGGCAGAGCTCGCCGAAGTTCCGACCGTCTGCGGCTCCTTGCGCGAAGCGCTCGAGGCGCTCGAAGCCGATCACGA
ATTCCTGCTCAAGGGCGATGTCTTCACCAAGGACCAGATCGACGCCTATGCGGAACTCAAGTGGGAAGAGGTCATGCGCA
CCGAAACCACGCCGTGCCCGGTCGAATTCGACATGTATTACAGCGCCTGA

Upstream 100 bases:

>100_bases
AAAGCGCGCTGCGCATTCGCACCGGCGAAAAAGACGACGAAGCCATCTGAATTTCAACCAATCGCGCCTGTAAGGCCGAA
CACCAGCAAGGAAATACTGC

Downstream 100 bases:

>100_bases
TCGCGTCGCTCTGATAATTCGAAAGGGCCCGGCGAAAGTCGGGCCCTTTTTCGTGCGCAAGCGCGCCACGGCTGCAAAAT
GCGGCGTTCGGGATACCGCG

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSSAKDIIKKIKEHEIEWVDLRFTDPKGKWQHLSMVASVMDEDALEDGLMFDGSSIAGWKAINESDMILKPDLSETWLDP
FSATPMLIVNCDIVEPSTGDWYSRDPRSTAKRAEAYLKSTGIGDTIYVGPEAEFFMFDDVRFEDGYAGSGFAIDDIELPT
NMGKEYEGGNMGHRPRAKGGYFPVAPVDSAMDIRAEMVATMLELGLPCDKHHHEVAAAQHELGLTFGTLVQTADRMQIYK
YVVHQVAHAYGKTATFMPKPIKEDNGSGMHTHMSIWEGDKPTFAGNEYAGLSENCLYYIGGVIKHAKALNAFTNPTTNSY
KRLVPGFEAPVLLAYSARNRSASCRIPYGAGDKAKRVEFRFPDAMANPYLAYSALLMAGLDGITNKIHPGDAMDKNLYDL
PPAELAEVPTVCGSLREALEALEADHEFLLKGDVFTKDQIDAYAELKWEEVMRTETTPCPVEFDMYYSA

Sequences:

>Translated_469_residues
MSSAKDIIKKIKEHEIEWVDLRFTDPKGKWQHLSMVASVMDEDALEDGLMFDGSSIAGWKAINESDMILKPDLSETWLDP
FSATPMLIVNCDIVEPSTGDWYSRDPRSTAKRAEAYLKSTGIGDTIYVGPEAEFFMFDDVRFEDGYAGSGFAIDDIELPT
NMGKEYEGGNMGHRPRAKGGYFPVAPVDSAMDIRAEMVATMLELGLPCDKHHHEVAAAQHELGLTFGTLVQTADRMQIYK
YVVHQVAHAYGKTATFMPKPIKEDNGSGMHTHMSIWEGDKPTFAGNEYAGLSENCLYYIGGVIKHAKALNAFTNPTTNSY
KRLVPGFEAPVLLAYSARNRSASCRIPYGAGDKAKRVEFRFPDAMANPYLAYSALLMAGLDGITNKIHPGDAMDKNLYDL
PPAELAEVPTVCGSLREALEALEADHEFLLKGDVFTKDQIDAYAELKWEEVMRTETTPCPVEFDMYYSA
>Mature_468_residues
SSAKDIIKKIKEHEIEWVDLRFTDPKGKWQHLSMVASVMDEDALEDGLMFDGSSIAGWKAINESDMILKPDLSETWLDPF
SATPMLIVNCDIVEPSTGDWYSRDPRSTAKRAEAYLKSTGIGDTIYVGPEAEFFMFDDVRFEDGYAGSGFAIDDIELPTN
MGKEYEGGNMGHRPRAKGGYFPVAPVDSAMDIRAEMVATMLELGLPCDKHHHEVAAAQHELGLTFGTLVQTADRMQIYKY
VVHQVAHAYGKTATFMPKPIKEDNGSGMHTHMSIWEGDKPTFAGNEYAGLSENCLYYIGGVIKHAKALNAFTNPTTNSYK
RLVPGFEAPVLLAYSARNRSASCRIPYGAGDKAKRVEFRFPDAMANPYLAYSALLMAGLDGITNKIHPGDAMDKNLYDLP
PAELAEVPTVCGSLREALEALEADHEFLLKGDVFTKDQIDAYAELKWEEVMRTETTPCPVEFDMYYSA

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI74271826, Length=347, Percent_Identity=27.0893371757925, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI74271837, Length=347, Percent_Identity=27.0893371757925, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI19923206, Length=347, Percent_Identity=27.0893371757925, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1790301, Length=467, Percent_Identity=63.169164882227, Blast_Score=609, Evalue=1e-175,
Organism=Escherichia coli, GI87081870, Length=390, Percent_Identity=26.6666666666667, Blast_Score=99, Evalue=7e-22,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52046; Mature: 51915

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAKDIIKKIKEHEIEWVDLRFTDPKGKWQHLSMVASVMDEDALEDGLMFDGSSIAGWK
CCCHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCE
AINESDMILKPDLSETWLDPFSATPMLIVNCDIVEPSTGDWYSRDPRSTAKRAEAYLKST
ECCCCCCEECCCCHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHC
GIGDTIYVGPEAEFFMFDDVRFEDGYAGSGFAIDDIELPTNMGKEYEGGNMGHRPRAKGG
CCCCEEEECCCCCEEEEECEEECCCCCCCCEEEECEECCCCCCCCCCCCCCCCCCCCCCC
YFPVAPVDSAMDIRAEMVATMLELGLPCDKHHHEVAAAQHELGLTFGTLVQTADRMQIYK
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
YVVHQVAHAYGKTATFMPKPIKEDNGSGMHTHMSIWEGDKPTFAGNEYAGLSENCLYYIG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHH
GVIKHAKALNAFTNPTTNSYKRLVPGFEAPVLLAYSARNRSASCRIPYGAGDKAKRVEFR
HHHHHHHHHHHHCCCCCCHHHHHCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCEEEEE
FPDAMANPYLAYSALLMAGLDGITNKIHPGDAMDKNLYDLPPAELAEVPTVCGSLREALE
CCHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ALEADHEFLLKGDVFTKDQIDAYAELKWEEVMRTETTPCPVEFDMYYSA
HHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECC
>Mature Secondary Structure 
SSAKDIIKKIKEHEIEWVDLRFTDPKGKWQHLSMVASVMDEDALEDGLMFDGSSIAGWK
CCHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCE
AINESDMILKPDLSETWLDPFSATPMLIVNCDIVEPSTGDWYSRDPRSTAKRAEAYLKST
ECCCCCCEECCCCHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHC
GIGDTIYVGPEAEFFMFDDVRFEDGYAGSGFAIDDIELPTNMGKEYEGGNMGHRPRAKGG
CCCCEEEECCCCCEEEEECEEECCCCCCCCEEEECEECCCCCCCCCCCCCCCCCCCCCCC
YFPVAPVDSAMDIRAEMVATMLELGLPCDKHHHEVAAAQHELGLTFGTLVQTADRMQIYK
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
YVVHQVAHAYGKTATFMPKPIKEDNGSGMHTHMSIWEGDKPTFAGNEYAGLSENCLYYIG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHH
GVIKHAKALNAFTNPTTNSYKRLVPGFEAPVLLAYSARNRSASCRIPYGAGDKAKRVEFR
HHHHHHHHHHHHCCCCCCHHHHHCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCEEEEE
FPDAMANPYLAYSALLMAGLDGITNKIHPGDAMDKNLYDLPPAELAEVPTVCGSLREALE
CCHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ALEADHEFLLKGDVFTKDQIDAYAELKWEEVMRTETTPCPVEFDMYYSA
HHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9171403 [H]