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The map label for this gene is rnhA

Identifier: 85373137

GI number: 85373137

Start: 354264

End: 354698

Strand: Reverse

Name: rnhA

Synonym: ELI_01550

Alternate gene names: 85373137

Gene position: 354698-354264 (Counterclockwise)

Preceding gene: 85373138

Following gene: 85373133

Centisome position: 11.62

GC content: 60.46

Gene sequence:

>435_bases
ATGAAGAAGGTCGAGATCTTCACCGATGGTGCCTGCAAGGGCAATCCCGGCCCTGGCGGCTGGGGCGTGCTGCTGCGTAT
GGGCAAACACGAGAAGGAATTGTCGGGCGGCGAGCCGGAGACGACCAACAACCGGATGGAACTGCGCGCGGCTATCGAAG
GCCTCAACGCCTTGATCGAGCCGTGCGAAGTCGAACTCTACACCGACAGCAAATATGTCGTCGACGGCATTACCAAGTGG
GTGCACGGCTGGAAAAAGCGGGGCTGGGTCAACGCCAGCAAAAAACCGGTGCGCAACGACGACCTGTGGCACGACCTAAT
CGAAGCCGAATTACGGCACAAAGTCACGTGGCACTGGGTCAAGGGTCATAACGGGCACGCCGAAAACGAACGGGCCGACC
GCCTCGCCAGCGAAGCGGCCGACCTGCAATCGTAA

Upstream 100 bases:

>100_bases
TCGATACGCCCGCCGATGCGCTGGTGACCCGCAAGGATCCGATGGATTTTGCGCGGCGGCTGCAGTTCTATCGCCGTGAC
GGCGAGAAAGTGTTCAACGC

Downstream 100 bases:

>100_bases
AGTCGGCGCAAATGCTCCGCGTGGTCCCAATCAGCTATTATCTTCGGTCGGTGCGTCCGGGCCATTTTTCTTGATCGAGT
CGATCGCATTCTGCGCACTC

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 144; Mature: 144

Protein sequence:

>144_residues
MKKVEIFTDGACKGNPGPGGWGVLLRMGKHEKELSGGEPETTNNRMELRAAIEGLNALIEPCEVELYTDSKYVVDGITKW
VHGWKKRGWVNASKKPVRNDDLWHDLIEAELRHKVTWHWVKGHNGHAENERADRLASEAADLQS

Sequences:

>Translated_144_residues
MKKVEIFTDGACKGNPGPGGWGVLLRMGKHEKELSGGEPETTNNRMELRAAIEGLNALIEPCEVELYTDSKYVVDGITKW
VHGWKKRGWVNASKKPVRNDDLWHDLIEAELRHKVTWHWVKGHNGHAENERADRLASEAADLQS
>Mature_144_residues
MKKVEIFTDGACKGNPGPGGWGVLLRMGKHEKELSGGEPETTNNRMELRAAIEGLNALIEPCEVELYTDSKYVVDGITKW
VHGWKKRGWVNASKKPVRNDDLWHDLIEAELRHKVTWHWVKGHNGHAENERADRLASEAADLQS

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=154, Percent_Identity=36.3636363636364, Blast_Score=73, Evalue=8e-14,
Organism=Escherichia coli, GI1786408, Length=140, Percent_Identity=62.1428571428571, Blast_Score=177, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI115532542, Length=142, Percent_Identity=33.8028169014084, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI17137476, Length=149, Percent_Identity=35.5704697986577, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45552509, Length=149, Percent_Identity=35.5704697986577, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_ERYLH (Q2ND39)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_457199.1
- ProteinModelPortal:   Q2ND39
- SMR:   Q2ND39
- STRING:   Q2ND39
- GeneID:   3871870
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_01550
- NMPDR:   fig|314225.3.peg.2779
- eggNOG:   COG0328
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- PhylomeDB:   Q2ND39
- BioCyc:   ELIT314225:ELI_01550-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 16249; Mature: 16249

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVEIFTDGACKGNPGPGGWGVLLRMGKHEKELSGGEPETTNNRMELRAAIEGLNALIE
CCEEEEEECCCCCCCCCCCCHHHHEECCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
PCEVELYTDSKYVVDGITKWVHGWKKRGWVNASKKPVRNDDLWHDLIEAELRHKVTWHWV
CCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCEEEEEE
KGHNGHAENERADRLASEAADLQS
CCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKVEIFTDGACKGNPGPGGWGVLLRMGKHEKELSGGEPETTNNRMELRAAIEGLNALIE
CCEEEEEECCCCCCCCCCCCHHHHEECCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
PCEVELYTDSKYVVDGITKWVHGWKKRGWVNASKKPVRNDDLWHDLIEAELRHKVTWHWV
CCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCEEEEEE
KGHNGHAENERADRLASEAADLQS
CCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA