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The map label for this gene is gatA [H]

Identifier: 85373051

GI number: 85373051

Start: 259705

End: 261231

Strand: Reverse

Name: gatA [H]

Synonym: ELI_01120

Alternate gene names: 85373051

Gene position: 261231-259705 (Counterclockwise)

Preceding gene: 85373052

Following gene: 85373050

Centisome position: 8.56

GC content: 68.24

Gene sequence:

>1527_bases
ATGAAGATTTTTGCACCGCTGATCGCCCTGACGCTCACCGCGCCTGTCCTCGCGCAGGAGGCCGCCGGTCTGCCGCCGCT
GCCCGGCACCAGCGCGGCGCAACAGGCGGCGGAGCAACAGCTGGCAAGGATTGCCACGCTTGACGATGCCGGGCCCGAGC
TCAATGCGGTCATCGTGGTCAACCCCGCCGCGCCCGCGCAGGCGCGCGCCGCCGAAGCGGCCGGCCTGCCGCTCGGCGGG
ATGACCGTGCTGGTGAAGGACAATATCGAGACCCGCGACATGCCGACGACGGCTGGCAGCCTGCTCCTCGCCGACAATGC
CACCGGCCGCGATGCCCCGCTCGTCGCGCGCATCCGCGCGGCGGGCGGCGTGATACTCGGCAAGACCAATCTCAGCGAAT
GGGCCAATTTCCGCGGCGACGATTCGACCAGCGGCTGGAGCGGGGTCGGCGGGCTGACAAGGAACCCGCACGCGACCGAC
CGCAACGCCTGTGGCTCGTCCTCGGGCAGCGGCGCGGCGATTGCCGCGGGCTTCGCGTGGGGCGCGATCGGGACGGAGAC
GAACGGCTCGATCACCTGCCCGGCCTCGATAAACGGGATCGTCGGTTTCAAGCCCACGGTCGGTTTCGTCAGCCGCACGC
ACGTGGTGCCGATCGCGGCGACGCAGGACACCGCCGGGCCGATGACCATCGACACCTACCGCGCGGCGATGCTGATGAAC
GCGATGACGGGCAGCGACCCGCTCGACCCGGTGACCGCCGAAGCCGACGCGCGCAAGGTCGATTTCACCGAGGGAATGCT
CGACGCCGGCCTCGCCGGGGTGCGTATCGGCGTGATGCGCGAACAGATCGGCAATCGCGCCGATGTCGCCGCGCTGTTCG
AGCAGGCGCTGACGGACATGGAGCGCGCTGGCGCGGTGCTGGTCGATATCGAGTTCAACCCCGACCGTGCGATGTTCCGC
GACAGCTTTGCCGTGCTGCTCTACGAATTCCGCGAAGGAATCGATGCCTACCTGCAAGCCCTGCCGCGCAACCGGGCCAG
CGATGCGCTGCTGCCGCGCAGCCTGCAGGAGCTGGTCGAGGGCAATGAAGCGGCTGGCGAAGCCGAGCTGCGCTGGTTCG
GGCAGCAATTGTTCGAACAGGCGCTCGAAGCGACCGACACCGCGGCCTATGAAACCGCGCGCGAAAACGCGGTCCGCATT
GCGGGCGAGGAAACGATCGACCGGCTGCTGGCCGATTTCGATGTCGAGTTCCTCGTCGCGCCCACTCGCGGCCCTGCCTG
GGTCAGCGACCTGGTGGTGGGAGACAATTTCAACAGTTCGATCGGCTTCGGCAGCCCGGCGGCCATTGCCGGCTATCCCC
ACCTGACGGTGCCGATGGGCCAAGTCGAAGGCCTGCCGGTCGGGCTTTCGATCATCGGCGCGAAGTGGGAAGATCACGCC
GTGCTGAAGGCGGGCAGCGCCTATGAAAGGATCCGCATGGCCGAACTGCCGGTCCCGACATTCCGTCCGTGGACCCCGGA
GCGTTAA

Upstream 100 bases:

>100_bases
GAAGTGCGCGACCGCCGCATCGGGCCGATCTTCCGCTTCAGCGTCAGCGGCAGCTTCTAAGGCCTTGCATCGTACCGCAG
GGGTGGCACTCTGCCGCGCG

Downstream 100 bases:

>100_bases
TCTGCGTTAATAGCGGGTATGCTCGATCTCGACCCGCGGATTATCATTCTCGCCCTGTTCGGCCTCGGGCTAATTCTCTC
CGTCGGCCTCGAGCGGTGGT

Product: amidase family protein

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MKIFAPLIALTLTAPVLAQEAAGLPPLPGTSAAQQAAEQQLARIATLDDAGPELNAVIVVNPAAPAQARAAEAAGLPLGG
MTVLVKDNIETRDMPTTAGSLLLADNATGRDAPLVARIRAAGGVILGKTNLSEWANFRGDDSTSGWSGVGGLTRNPHATD
RNACGSSSGSGAAIAAGFAWGAIGTETNGSITCPASINGIVGFKPTVGFVSRTHVVPIAATQDTAGPMTIDTYRAAMLMN
AMTGSDPLDPVTAEADARKVDFTEGMLDAGLAGVRIGVMREQIGNRADVAALFEQALTDMERAGAVLVDIEFNPDRAMFR
DSFAVLLYEFREGIDAYLQALPRNRASDALLPRSLQELVEGNEAAGEAELRWFGQQLFEQALEATDTAAYETARENAVRI
AGEETIDRLLADFDVEFLVAPTRGPAWVSDLVVGDNFNSSIGFGSPAAIAGYPHLTVPMGQVEGLPVGLSIIGAKWEDHA
VLKAGSAYERIRMAELPVPTFRPWTPER

Sequences:

>Translated_508_residues
MKIFAPLIALTLTAPVLAQEAAGLPPLPGTSAAQQAAEQQLARIATLDDAGPELNAVIVVNPAAPAQARAAEAAGLPLGG
MTVLVKDNIETRDMPTTAGSLLLADNATGRDAPLVARIRAAGGVILGKTNLSEWANFRGDDSTSGWSGVGGLTRNPHATD
RNACGSSSGSGAAIAAGFAWGAIGTETNGSITCPASINGIVGFKPTVGFVSRTHVVPIAATQDTAGPMTIDTYRAAMLMN
AMTGSDPLDPVTAEADARKVDFTEGMLDAGLAGVRIGVMREQIGNRADVAALFEQALTDMERAGAVLVDIEFNPDRAMFR
DSFAVLLYEFREGIDAYLQALPRNRASDALLPRSLQELVEGNEAAGEAELRWFGQQLFEQALEATDTAAYETARENAVRI
AGEETIDRLLADFDVEFLVAPTRGPAWVSDLVVGDNFNSSIGFGSPAAIAGYPHLTVPMGQVEGLPVGLSIIGAKWEDHA
VLKAGSAYERIRMAELPVPTFRPWTPER
>Mature_508_residues
MKIFAPLIALTLTAPVLAQEAAGLPPLPGTSAAQQAAEQQLARIATLDDAGPELNAVIVVNPAAPAQARAAEAAGLPLGG
MTVLVKDNIETRDMPTTAGSLLLADNATGRDAPLVARIRAAGGVILGKTNLSEWANFRGDDSTSGWSGVGGLTRNPHATD
RNACGSSSGSGAAIAAGFAWGAIGTETNGSITCPASINGIVGFKPTVGFVSRTHVVPIAATQDTAGPMTIDTYRAAMLMN
AMTGSDPLDPVTAEADARKVDFTEGMLDAGLAGVRIGVMREQIGNRADVAALFEQALTDMERAGAVLVDIEFNPDRAMFR
DSFAVLLYEFREGIDAYLQALPRNRASDALLPRSLQELVEGNEAAGEAELRWFGQQLFEQALEATDTAAYETARENAVRI
AGEETIDRLLADFDVEFLVAPTRGPAWVSDLVVGDNFNSSIGFGSPAAIAGYPHLTVPMGQVEGLPVGLSIIGAKWEDHA
VLKAGSAYERIRMAELPVPTFRPWTPER

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=492, Percent_Identity=26.6260162601626, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI195972892, Length=426, Percent_Identity=23.7089201877934, Blast_Score=82, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17543272, Length=455, Percent_Identity=26.1538461538462, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17537465, Length=174, Percent_Identity=29.8850574712644, Blast_Score=69, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=433, Percent_Identity=26.0969976905312, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323950, Length=230, Percent_Identity=29.1304347826087, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24648113, Length=468, Percent_Identity=29.4871794871795, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24652985, Length=528, Percent_Identity=25, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922090, Length=528, Percent_Identity=25, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24652981, Length=528, Percent_Identity=25, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24652983, Length=528, Percent_Identity=25, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24644968, Length=505, Percent_Identity=23.1683168316832, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648435, Length=179, Percent_Identity=32.4022346368715, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24648437, Length=179, Percent_Identity=32.4022346368715, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI45550774, Length=179, Percent_Identity=32.4022346368715, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24648441, Length=183, Percent_Identity=32.2404371584699, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648439, Length=183, Percent_Identity=32.2404371584699, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 53261; Mature: 53261

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFAPLIALTLTAPVLAQEAAGLPPLPGTSAAQQAAEQQLARIATLDDAGPELNAVIVV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
NPAAPAQARAAEAAGLPLGGMTVLVKDNIETRDMPTTAGSLLLADNATGRDAPLVARIRA
CCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCCCCCCHHEEEHH
AGGVILGKTNLSEWANFRGDDSTSGWSGVGGLTRNPHATDRNACGSSSGSGAAIAAGFAW
CCCEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECEEE
GAIGTETNGSITCPASINGIVGFKPTVGFVSRTHVVPIAATQDTAGPMTIDTYRAAMLMN
CCCCCCCCCCEECCCCCCCEEECCCCCCCCCCCEEEEEEECCCCCCCEEHHHHHHHHHHH
AMTGSDPLDPVTAEADARKVDFTEGMLDAGLAGVRIGVMREQIGNRADVAALFEQALTDM
HHCCCCCCCCCCCCCCCCEECHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ERAGAVLVDIEFNPDRAMFRDSFAVLLYEFREGIDAYLQALPRNRASDALLPRSLQELVE
HHCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
GNEAAGEAELRWFGQQLFEQALEATDTAAYETARENAVRIAGEETIDRLLADFDVEFLVA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHHHHCCCEEEEE
PTRGPAWVSDLVVGDNFNSSIGFGSPAAIAGYPHLTVPMGQVEGLPVGLSIIGAKWEDHA
CCCCCHHHHHEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCEECEEEEECCCCCCC
VLKAGSAYERIRMAELPVPTFRPWTPER
EEECCCHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKIFAPLIALTLTAPVLAQEAAGLPPLPGTSAAQQAAEQQLARIATLDDAGPELNAVIVV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
NPAAPAQARAAEAAGLPLGGMTVLVKDNIETRDMPTTAGSLLLADNATGRDAPLVARIRA
CCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCCCCCCHHEEEHH
AGGVILGKTNLSEWANFRGDDSTSGWSGVGGLTRNPHATDRNACGSSSGSGAAIAAGFAW
CCCEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECEEE
GAIGTETNGSITCPASINGIVGFKPTVGFVSRTHVVPIAATQDTAGPMTIDTYRAAMLMN
CCCCCCCCCCEECCCCCCCEEECCCCCCCCCCCEEEEEEECCCCCCCEEHHHHHHHHHHH
AMTGSDPLDPVTAEADARKVDFTEGMLDAGLAGVRIGVMREQIGNRADVAALFEQALTDM
HHCCCCCCCCCCCCCCCCEECHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ERAGAVLVDIEFNPDRAMFRDSFAVLLYEFREGIDAYLQALPRNRASDALLPRSLQELVE
HHCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
GNEAAGEAELRWFGQQLFEQALEATDTAAYETARENAVRIAGEETIDRLLADFDVEFLVA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHHHHCCCEEEEE
PTRGPAWVSDLVVGDNFNSSIGFGSPAAIAGYPHLTVPMGQVEGLPVGLSIIGAKWEDHA
CCCCCHHHHHEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCEECEEEEECCCCCCC
VLKAGSAYERIRMAELPVPTFRPWTPER
EEECCCHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA