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The map label for this gene is tilS [H]

Identifier: 85372930

GI number: 85372930

Start: 133108

End: 134067

Strand: Direct

Name: tilS [H]

Synonym: ELI_00515

Alternate gene names: 85372930

Gene position: 133108-134067 (Clockwise)

Preceding gene: 85372929

Following gene: 85372942

Centisome position: 4.36

GC content: 67.4

Gene sequence:

>960_bases
TTGCGTCCGCTGTGGCACGATATCGACGATGACGACGCGCCGAGGCTCGGCGTCGCCGTGTCCGGCGGACCGGACAGTCT
CGCCCTGTTGCTGCTCGCCAATACGGCTCTGCCTGGCCGCGTCGTCGCCGCGACCATCGACCACGGCTTGCGGCCCGAGG
CGGCCGACGAAGCAGCCATGGTCGGCGGGCTGTGCGAGCGGTTGGGAGTCCTGCACCACACGGTGGAAGTGGCGCTCGAA
CCCGGCAATACGCAGGATCGCGCAAGAAAGGCGCGTTACGAGGCGCTGTGCCGATGCTTCGGCGAACAAGGCGCCGACAC
TTTTGCCACCGCCCATCATGCCGACGACCAGGCCGAAACCCTGCTGATGCGCTTCAACCGCGGCAGCGGGTTATCGGGGC
TGGCGGGCATCCGTGCGCGCACCGTGCTGTTGTCGCCCGAGCCGCTGGGCGAATATCTCGTCGTGCGACCCTTGCTCGAG
TGGCGGCGCGAAGAACTGGCGGGGATCGTGGCGGATGCCGGGCTTGCCCCGGTGCAGGACCCGTCCAATGTCGATACGCG
CTATGACAGAGTTCAGGTCCGGCGGGCCTTGGCCGATCTACCGTGGCTCGATCCGCTGGCGATGGCGCGCAGCGCGCGCC
ATCTGCAGGAGGCGGAGGATGCAGTGCGCCGGTCATCTGCCGATGTCTACAATCGTTGCACCTACCGCGAGGGCGAAACG
GTCTGGTTTCACTGGGGCCACGCCCGGACCGTAGAGATCGAAGCGGTGCGGCTGATCTTGCTGGAATTCGGGGCCGACGT
CCCGCGATCGTCGATCGCGCGGCTGATCGACCAGCTCATGTCAGAGGGTGCGGGCTCGCTTGGCGGCGTAGTCGCGCGGC
GTGCTTGGCATCGGGATGGCCCCAATTCTGAGACCGATGCGTGGAAGTTCGAGAAGGAGCCGCCGCGCCGCACCGGATGA

Upstream 100 bases:

>100_bases
CAGGACCAGTACGAGGCCAACCGGGCCAAGGTGAGCTGCAATTGATAGCACGCCCGATCTTGCGGTCGACCGGGCCTTAG
CCGAGCGCTTCCTCGCGGAT

Downstream 100 bases:

>100_bases
TGCAGCGAGCGTGCAGGCAGATTACAGGATCGATTCACCCATTCCCAGCGTCTCGCCATCGGTAATGATGACCTGGTCGC
CCTTCCCGGCGACGGCGAAG

Product: ATPase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MRPLWHDIDDDDAPRLGVAVSGGPDSLALLLLANTALPGRVVAATIDHGLRPEAADEAAMVGGLCERLGVLHHTVEVALE
PGNTQDRARKARYEALCRCFGEQGADTFATAHHADDQAETLLMRFNRGSGLSGLAGIRARTVLLSPEPLGEYLVVRPLLE
WRREELAGIVADAGLAPVQDPSNVDTRYDRVQVRRALADLPWLDPLAMARSARHLQEAEDAVRRSSADVYNRCTYREGET
VWFHWGHARTVEIEAVRLILLEFGADVPRSSIARLIDQLMSEGAGSLGGVVARRAWHRDGPNSETDAWKFEKEPPRRTG

Sequences:

>Translated_319_residues
MRPLWHDIDDDDAPRLGVAVSGGPDSLALLLLANTALPGRVVAATIDHGLRPEAADEAAMVGGLCERLGVLHHTVEVALE
PGNTQDRARKARYEALCRCFGEQGADTFATAHHADDQAETLLMRFNRGSGLSGLAGIRARTVLLSPEPLGEYLVVRPLLE
WRREELAGIVADAGLAPVQDPSNVDTRYDRVQVRRALADLPWLDPLAMARSARHLQEAEDAVRRSSADVYNRCTYREGET
VWFHWGHARTVEIEAVRLILLEFGADVPRSSIARLIDQLMSEGAGSLGGVVARRAWHRDGPNSETDAWKFEKEPPRRTG
>Mature_319_residues
MRPLWHDIDDDDAPRLGVAVSGGPDSLALLLLANTALPGRVVAATIDHGLRPEAADEAAMVGGLCERLGVLHHTVEVALE
PGNTQDRARKARYEALCRCFGEQGADTFATAHHADDQAETLLMRFNRGSGLSGLAGIRARTVLLSPEPLGEYLVVRPLLE
WRREELAGIVADAGLAPVQDPSNVDTRYDRVQVRRALADLPWLDPLAMARSARHLQEAEDAVRRSSADVYNRCTYREGET
VWFHWGHARTVEIEAVRLILLEFGADVPRSSIARLIDQLMSEGAGSLGGVVARRAWHRDGPNSETDAWKFEKEPPRRTG

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=183, Percent_Identity=34.4262295081967, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 35037; Mature: 35037

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPLWHDIDDDDAPRLGVAVSGGPDSLALLLLANTALPGRVVAATIDHGLRPEAADEAAM
CCCCCCCCCCCCCCCEEEEECCCCHHHEEHHHHCCCCCCCEEEEECCCCCCCCCHHHHHH
VGGLCERLGVLHHTVEVALEPGNTQDRARKARYEALCRCFGEQGADTFATAHHADDQAET
HHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHH
LLMRFNRGSGLSGLAGIRARTVLLSPEPLGEYLVVRPLLEWRREELAGIVADAGLAPVQD
HHHHCCCCCCCCCHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PSNVDTRYDRVQVRRALADLPWLDPLAMARSARHLQEAEDAVRRSSADVYNRCTYREGET
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCE
VWFHWGHARTVEIEAVRLILLEFGADVPRSSIARLIDQLMSEGAGSLGGVVARRAWHRDG
EEEECCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
PNSETDAWKFEKEPPRRTG
CCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRPLWHDIDDDDAPRLGVAVSGGPDSLALLLLANTALPGRVVAATIDHGLRPEAADEAAM
CCCCCCCCCCCCCCCEEEEECCCCHHHEEHHHHCCCCCCCEEEEECCCCCCCCCHHHHHH
VGGLCERLGVLHHTVEVALEPGNTQDRARKARYEALCRCFGEQGADTFATAHHADDQAET
HHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHH
LLMRFNRGSGLSGLAGIRARTVLLSPEPLGEYLVVRPLLEWRREELAGIVADAGLAPVQD
HHHHCCCCCCCCCHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PSNVDTRYDRVQVRRALADLPWLDPLAMARSARHLQEAEDAVRRSSADVYNRCTYREGET
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCE
VWFHWGHARTVEIEAVRLILLEFGADVPRSSIARLIDQLMSEGAGSLGGVVARRAWHRDG
EEEECCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
PNSETDAWKFEKEPPRRTG
CCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA