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The map label for this gene is ptsP [H]

Identifier: 85372927

GI number: 85372927

Start: 128718

End: 130985

Strand: Direct

Name: ptsP [H]

Synonym: ELI_00500

Alternate gene names: 85372927

Gene position: 128718-130985 (Clockwise)

Preceding gene: 85372926

Following gene: 85372928

Centisome position: 4.22

GC content: 64.59

Gene sequence:

>2268_bases
ATGAGTGCTGCCGCCTCCGCCCGCCAGATCCTGACCGGCCTGCACGAGGTCATGGCCTCGCGCATGCATGCGCAGGGCAA
GCTCGACCGCGTGGTCGAGATCATCGGCGAATCGCTCGATAGCGAAGTGTGCTCGATCTACCTGCTGCGCGAAGGCATGC
TGGAGCTTTACGCGACCCGCGGCCTAAACAAGGAGGCTGTCCACGTCACGCGCATGGCAATCGGCGAGGGCCTGACCGGC
ACGTTGGTCGCCAACCAGGAAACGTTGAACCTCGCCGAAGCGCGCGCGCATCCCGATTTCCAGTACCGGCCCGAAACCGG
CGAAGAGAAGTTTCACTCCTTTGCCGGTGTTCCGATCGTCTATCGCGAACGTGCCGTGGGCGCGCTCAACGTGCAGCACA
TGGATCCGCGCAAATACGAAGACGTCGAGATCGAGGCGCTGCAGACGACGGCCATGGTCCTTTCCGAACTGATCGGCGCG
GCGGAACTGATCGACGAGGAAGAGGTCGGTGCCACCGAGGCGCAGACCGGACCGGCGCAGATCGAAGGGCTATCGCTCGT
TTCGGGAATCGCCAGCGGTTTCGCGGTGTTCCACCAGCCGCGGGTGACTATCGACCAGGTCGTGGCCGATGACATCGAGG
TCGAGCGGCAGCGCGTCTATCACGCCTTCGACAAGATGCGCGACCAGATCGACGGGCTCTCGCAGCAGGCCGAATTCGGC
AAGGGCGGCGAGCACGAGGACATCCTCGCGACATACAGGATGTTCGCTTACGACGAAGGCTGGTCGAGGCGCATCAACGA
AGCCATCGACAGTGGGCTGACGGCGGAAGCCGCGATCGAGCGCGTGCAGCAGCGAACCCGGATGCGCATGCGCGAGATCG
ACGATCCGCTGCTGGCCGAGCGCATGCATGATCTGGAAGATTTGTCCAACCGGTTGCTGCGGATCGTCTCGGGCCAGCTC
GGCACGGCGGCCTCGCAGGGCCTGCGGCGCGACACGATCCTGATCGCGCGCAATCTCGGCCCGGCCGAGCTGCTCGAATA
CGACCGTCGGCGCCTGAAGGGCGTGATCCTAGAGGAAGGCTCGCTCACCGCGCATGTCGTGATCGTGGCGCGCGCGATGG
GTATCCCGGTCCTCGGGCGGGTTCGCGGCTTGCGCGGGGTGGTGCGCGAAGGCGACGAAATTCTGCTCGACAGCGATGCC
GGCATGGCCAACATCCGGCCGACGCAGCCGGTCGCCGATGCCTTCGACACACGCTTCGTGAAGAGCAAGGAACGCCAGGC
CGCCTATGCCGAGTTGCGCGATGTCGAGCCGTTCACGCGCTGCGGCACCCGCATCCAGGTACTGATGAACGCCGGCCTGC
GCGAGGATATGTCGAACCTGCCGTTGGTGGGCGCCGACGGGGTGGGGCTGTTCCGCACCGAATTCCAGTTTCTCGTTTCC
GCCACCCTGCCGCAGCGGGAGCGGCAGACCCGGCTCTATCGCGACGTTCTCGATGCGGCGGGCGGAAAGGAAGTGGTCTT
TCGCACTGTCGATATCGGCGGCGACAAGGCGGTCCCGTATCTCGCTTCGGAGGAGGCAGAGAACGACGAGAATCCCGCAA
TGGGCTGGCGCGCATTGCGGCTCGCGCTGGAACGCGAAGGCCTGCTCAAGGCGCAGGCGCGCGCGCTGCTGGAAGCTTCG
GCCGGCAAGAAGCTCAACGTTATGTTCCCGATGGTCAGCGAACCGTGGGAATTCGATGCCGCCAAGGCTGTGTTCGACGA
CCAGATCGCTTTCCTGCGCAAGCAGAAGAAGATGCTGCCGGATGAAATCAATTTCGGCGCCATGCTGGAAGTGCCTTCGC
TGGCCGAAGTGCTCGATCTCCTCATCCCGAAAGTGTCCTTCCTCTCGATCGGCACCAACGACCTGACGCAATTCCTGTTT
GCCGCCGATCGGGCCAATCCGAAACTGGCGGCGCGCTATGACTGGCTCAGCCCCGCAATCCTGAGATTCCTGCGCCGGAT
CGTACAGGCGACCACCGGACACAATGTCGGTCTCGGCGTGTGCGGCGAGATGGGCGGCCGCAGGCTGGAGGCGCTGGCAC
TGCTGGGCCTCGGCATCCACCGGCTTTCGATTACGCCCGTCTCGGTCGGCCCGATCAAGGAACTGGTACGGCAGGTCGAC
CTCAAGCAGATCGAGGATGCGATGAACGGCTGGCTCGCCTCGCCGCCGCCTTCCATGCGGGAGGCGATTACCGCATGGGC
GCGCGAGCGGGACATCGATGTAGAGTGA

Upstream 100 bases:

>100_bases
GGAGATCGCTGCGGGCTGATCCCTCCGCCCGCTCGCGGGCCCTTGTAAATTACCGACACGGCAATTCCCATGGCGAGATC
GATGCGCTAGGCGCAGCAGT

Downstream 100 bases:

>100_bases
TATGCGGCGGAAAGTGGCGGCAGACTGTTCAAGCGCAGTCGCGGCGGCAATCATCGCTTGACAGGCACCGTTAAGCCCGC
GGATGGTATTGGGCAAAGAT

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 755; Mature: 754

Protein sequence:

>755_residues
MSAAASARQILTGLHEVMASRMHAQGKLDRVVEIIGESLDSEVCSIYLLREGMLELYATRGLNKEAVHVTRMAIGEGLTG
TLVANQETLNLAEARAHPDFQYRPETGEEKFHSFAGVPIVYRERAVGALNVQHMDPRKYEDVEIEALQTTAMVLSELIGA
AELIDEEEVGATEAQTGPAQIEGLSLVSGIASGFAVFHQPRVTIDQVVADDIEVERQRVYHAFDKMRDQIDGLSQQAEFG
KGGEHEDILATYRMFAYDEGWSRRINEAIDSGLTAEAAIERVQQRTRMRMREIDDPLLAERMHDLEDLSNRLLRIVSGQL
GTAASQGLRRDTILIARNLGPAELLEYDRRRLKGVILEEGSLTAHVVIVARAMGIPVLGRVRGLRGVVREGDEILLDSDA
GMANIRPTQPVADAFDTRFVKSKERQAAYAELRDVEPFTRCGTRIQVLMNAGLREDMSNLPLVGADGVGLFRTEFQFLVS
ATLPQRERQTRLYRDVLDAAGGKEVVFRTVDIGGDKAVPYLASEEAENDENPAMGWRALRLALEREGLLKAQARALLEAS
AGKKLNVMFPMVSEPWEFDAAKAVFDDQIAFLRKQKKMLPDEINFGAMLEVPSLAEVLDLLIPKVSFLSIGTNDLTQFLF
AADRANPKLAARYDWLSPAILRFLRRIVQATTGHNVGLGVCGEMGGRRLEALALLGLGIHRLSITPVSVGPIKELVRQVD
LKQIEDAMNGWLASPPPSMREAITAWARERDIDVE

Sequences:

>Translated_755_residues
MSAAASARQILTGLHEVMASRMHAQGKLDRVVEIIGESLDSEVCSIYLLREGMLELYATRGLNKEAVHVTRMAIGEGLTG
TLVANQETLNLAEARAHPDFQYRPETGEEKFHSFAGVPIVYRERAVGALNVQHMDPRKYEDVEIEALQTTAMVLSELIGA
AELIDEEEVGATEAQTGPAQIEGLSLVSGIASGFAVFHQPRVTIDQVVADDIEVERQRVYHAFDKMRDQIDGLSQQAEFG
KGGEHEDILATYRMFAYDEGWSRRINEAIDSGLTAEAAIERVQQRTRMRMREIDDPLLAERMHDLEDLSNRLLRIVSGQL
GTAASQGLRRDTILIARNLGPAELLEYDRRRLKGVILEEGSLTAHVVIVARAMGIPVLGRVRGLRGVVREGDEILLDSDA
GMANIRPTQPVADAFDTRFVKSKERQAAYAELRDVEPFTRCGTRIQVLMNAGLREDMSNLPLVGADGVGLFRTEFQFLVS
ATLPQRERQTRLYRDVLDAAGGKEVVFRTVDIGGDKAVPYLASEEAENDENPAMGWRALRLALEREGLLKAQARALLEAS
AGKKLNVMFPMVSEPWEFDAAKAVFDDQIAFLRKQKKMLPDEINFGAMLEVPSLAEVLDLLIPKVSFLSIGTNDLTQFLF
AADRANPKLAARYDWLSPAILRFLRRIVQATTGHNVGLGVCGEMGGRRLEALALLGLGIHRLSITPVSVGPIKELVRQVD
LKQIEDAMNGWLASPPPSMREAITAWARERDIDVE
>Mature_754_residues
SAAASARQILTGLHEVMASRMHAQGKLDRVVEIIGESLDSEVCSIYLLREGMLELYATRGLNKEAVHVTRMAIGEGLTGT
LVANQETLNLAEARAHPDFQYRPETGEEKFHSFAGVPIVYRERAVGALNVQHMDPRKYEDVEIEALQTTAMVLSELIGAA
ELIDEEEVGATEAQTGPAQIEGLSLVSGIASGFAVFHQPRVTIDQVVADDIEVERQRVYHAFDKMRDQIDGLSQQAEFGK
GGEHEDILATYRMFAYDEGWSRRINEAIDSGLTAEAAIERVQQRTRMRMREIDDPLLAERMHDLEDLSNRLLRIVSGQLG
TAASQGLRRDTILIARNLGPAELLEYDRRRLKGVILEEGSLTAHVVIVARAMGIPVLGRVRGLRGVVREGDEILLDSDAG
MANIRPTQPVADAFDTRFVKSKERQAAYAELRDVEPFTRCGTRIQVLMNAGLREDMSNLPLVGADGVGLFRTEFQFLVSA
TLPQRERQTRLYRDVLDAAGGKEVVFRTVDIGGDKAVPYLASEEAENDENPAMGWRALRLALEREGLLKAQARALLEASA
GKKLNVMFPMVSEPWEFDAAKAVFDDQIAFLRKQKKMLPDEINFGAMLEVPSLAEVLDLLIPKVSFLSIGTNDLTQFLFA
ADRANPKLAARYDWLSPAILRFLRRIVQATTGHNVGLGVCGEMGGRRLEALALLGLGIHRLSITPVSVGPIKELVRQVDL
KQIEDAMNGWLASPPPSMREAITAWARERDIDVE

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=735, Percent_Identity=32.6530612244898, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1788756, Length=559, Percent_Identity=32.5581395348837, Blast_Score=287, Evalue=2e-78,
Organism=Escherichia coli, GI1788726, Length=587, Percent_Identity=33.0494037478705, Blast_Score=254, Evalue=2e-68,
Organism=Escherichia coli, GI48994992, Length=497, Percent_Identity=31.9919517102616, Blast_Score=225, Evalue=9e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 83560; Mature: 83429

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAASARQILTGLHEVMASRMHAQGKLDRVVEIIGESLDSEVCSIYLLREGMLELYATR
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLNKEAVHVTRMAIGEGLTGTLVANQETLNLAEARAHPDFQYRPETGEEKFHSFAGVPIV
CCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCEE
YRERAVGALNVQHMDPRKYEDVEIEALQTTAMVLSELIGAAELIDEEEVGATEAQTGPAQ
ECCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCHH
IEGLSLVSGIASGFAVFHQPRVTIDQVVADDIEVERQRVYHAFDKMRDQIDGLSQQAEFG
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCC
KGGEHEDILATYRMFAYDEGWSRRINEAIDSGLTAEAAIERVQQRTRMRMREIDDPLLAE
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHH
RMHDLEDLSNRLLRIVSGQLGTAASQGLRRDTILIARNLGPAELLEYDRRRLKGVILEEG
HHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCEEEEECC
SLTAHVVIVARAMGIPVLGRVRGLRGVVREGDEILLDSDAGMANIRPTQPVADAFDTRFV
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
KSKERQAAYAELRDVEPFTRCGTRIQVLMNAGLREDMSNLPLVGADGVGLFRTEFQFLVS
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHCCCEECCCCCCHHHHHHHHHHH
ATLPQRERQTRLYRDVLDAAGGKEVVFRTVDIGGDKAVPYLASEEAENDENPAMGWRALR
HCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LALEREGLLKAQARALLEASAGKKLNVMFPMVSEPWEFDAAKAVFDDQIAFLRKQKKMLP
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
DEINFGAMLEVPSLAEVLDLLIPKVSFLSIGTNDLTQFLFAADRANPKLAARYDWLSPAI
CCCCCCCEECCCCHHHHHHHHHCCHHEEECCHHHHHHHHHHHCCCCCCCEECCCCCCHHH
LRFLRRIVQATTGHNVGLGVCGEMGGRRLEALALLGLGIHRLSITPVSVGPIKELVRQVD
HHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHCHHEEEECCCCCHHHHHHHHHHH
LKQIEDAMNGWLASPPPSMREAITAWARERDIDVE
HHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SAAASARQILTGLHEVMASRMHAQGKLDRVVEIIGESLDSEVCSIYLLREGMLELYATR
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLNKEAVHVTRMAIGEGLTGTLVANQETLNLAEARAHPDFQYRPETGEEKFHSFAGVPIV
CCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCEE
YRERAVGALNVQHMDPRKYEDVEIEALQTTAMVLSELIGAAELIDEEEVGATEAQTGPAQ
ECCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCHH
IEGLSLVSGIASGFAVFHQPRVTIDQVVADDIEVERQRVYHAFDKMRDQIDGLSQQAEFG
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCC
KGGEHEDILATYRMFAYDEGWSRRINEAIDSGLTAEAAIERVQQRTRMRMREIDDPLLAE
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHH
RMHDLEDLSNRLLRIVSGQLGTAASQGLRRDTILIARNLGPAELLEYDRRRLKGVILEEG
HHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCEEEEECC
SLTAHVVIVARAMGIPVLGRVRGLRGVVREGDEILLDSDAGMANIRPTQPVADAFDTRFV
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
KSKERQAAYAELRDVEPFTRCGTRIQVLMNAGLREDMSNLPLVGADGVGLFRTEFQFLVS
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHCCCEECCCCCCHHHHHHHHHHH
ATLPQRERQTRLYRDVLDAAGGKEVVFRTVDIGGDKAVPYLASEEAENDENPAMGWRALR
HCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LALEREGLLKAQARALLEASAGKKLNVMFPMVSEPWEFDAAKAVFDDQIAFLRKQKKMLP
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
DEINFGAMLEVPSLAEVLDLLIPKVSFLSIGTNDLTQFLFAADRANPKLAARYDWLSPAI
CCCCCCCEECCCCHHHHHHHHHCCHHEEECCHHHHHHHHHHHCCCCCCCEECCCCCCHHH
LRFLRRIVQATTGHNVGLGVCGEMGGRRLEALALLGLGIHRLSITPVSVGPIKELVRQVD
HHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHCHHEEEECCCCCHHHHHHHHHHH
LKQIEDAMNGWLASPPPSMREAITAWARERDIDVE
HHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]