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The map label for this gene is opuD [H]

Identifier: 85372920

GI number: 85372920

Start: 122582

End: 124288

Strand: Reverse

Name: opuD [H]

Synonym: ELI_00465

Alternate gene names: 85372920

Gene position: 124288-122582 (Counterclockwise)

Preceding gene: 85372921

Following gene: 85372919

Centisome position: 4.07

GC content: 63.39

Gene sequence:

>1707_bases
GTGACTTCCGACACCGATACCGCCGTTTCGCAACCTGCGATCGACCCGCCACTGTGCGAGCTTCCCGTGGCGACGGCCGA
GCGGGGTTTCTACACCGGCTTCAGCACCCACGTGGCCATCCCGGCCAAGATTGTCGTCAGCGTGCTGATTGCCTGGGCAA
TCTTCTTCCCCACCCATGCAAGCGACACGCTGGCCATCGCCAACCAGACGATCATCCGCCAGTTCGCCTTCTGGTATGTC
TATCTCGTCGCGTTCCTGGTGGTGGTCTGCCTCGTGCTGGCGCTGGTGCCGCAATCGGGCAGCCTCAGGATCGGCGCGCC
GGGCGAAAAGCCGGAATTTTCCCGCTTCTCGTGGTTCTCCATGCTGTTCGGAGCCGGGATCGGTATCGGCATGTTGACCT
ATTCCACCGGTGAGCCGCTGGCGCATTTCCAGAACAATCCCGACATTATTCGCGGTCTGGTCGAACCGGCATCCGCTGCC
GCCGTCCCGTCGGCTTACGAATATACCTTTCTGCACTGGGGCCTGTCTGCCTGGGCGACCTATGCCTTGGTCGGGCTCGC
GGTCGGCTATGTCGCGTACCGCCGCAACCTGCCGCTGACGATCCGGTCCAGCCTCGCGCCTATCTTCGGGTTGCGGCTGT
CGGGCAATTCGGGTCACCTGGTCGACATCGTCGCGGTGGTCGCCACGATCCTCGGCGTGTCGGTGACCATGGGTCTCGGC
GTCGAGCAATTCGTTGCCGGGCTGTATCGGCTCGGGTTCGGCGAGTGGTTGATCAAGGAGGGCGGTACGGCATCGGACTG
GGCCATCGTGCTGGCGCTGGTCCTGCTCGTCGGCGCATCGACAATCTCGGCGCTGTCCGGTGTCGGTCGCGGGATCAAAT
GGCTGTCCAATCTCAACATGGGCCTGTCGTTCCTGCTGCTTGCGGTGTTCCTCGTCTTCGGTGCCGGGCTCTATGGGTTG
CAATTGCTCGGCGAGGGTCTTGTCGACCATGTGGCGCGGCTGCCCGAGCGAATGCTGGCGTTGTGGACTGCCGACGGCAC
CGAGACCGGCAATGCGCTGGCGCAATGGCAGCTCGACTGGTCGGTGTTCTACTGGGCGTGGTGGATCGCATTCGCGCCCT
TCGTCGGGATGTTCATCGCCCGCATTTCGCGCGGGCGCACAGTGCGCGAATACGTGCTCGGCGTGATCCTCGTTCCCTCG
CTGATGTGTTTCCTCTGGATGGCGCTGGCGGGCGGGACCGCGATCCAGCTGGAACTCGACGGAACGGCGCAAGGCGCGAT
CCTGTCGGCAGGGATCTCGGACCAGATCTATGCCACCATCACCGCGTTGATGAGCCCGGGCGTCGCTTACCTCCTCTCCG
GCCTGATCGTGATCCTGCTGATGACCTATCTCATCACATCGGCCGACAGCGCGATCCTGATCGTCAACACGATCAACGGC
GCGGGCGAAAACGACGGCAGCCGCAATCGCCACATCCTGTTCTGGGGCGCGGCGCTGGCACTGGTGGTCGGCAGCATGCT
GATCCTCGGAGGGATCGATGCCATCCGCATCACCATGATCATCGGCGCGCTGCCTTTCTCGGTTGTCGTAGCGCTTATGG
CGATCTCGATCGTCAAGGCAATCGTGTACGATGTCATCCGCAAGCGGCACGGCGTCCAGACCCACTGCGAGGATATCCTG
GCCGAAGCCGCAGCAAGAGCCGAGTGA

Upstream 100 bases:

>100_bases
ACACGAAAGCCCGGTTTGCATGAATGCGTTGGCCGGAAACTGGGTGATGCCTGTTGCCTCGATCCCCGGCGGCGTGCGCG
CCTGACGACGAGGACCTTGA

Downstream 100 bases:

>100_bases
AGGCTATCCATCGGAAACTTGACGTTACATGACAATGACTTGCGATGTTACTAGAATCGCCACTTGGCGTTTTTAAGTAA
AAAAAGCTGACAATTTGATT

Product: transporter, BCCT family protein

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MTSDTDTAVSQPAIDPPLCELPVATAERGFYTGFSTHVAIPAKIVVSVLIAWAIFFPTHASDTLAIANQTIIRQFAFWYV
YLVAFLVVVCLVLALVPQSGSLRIGAPGEKPEFSRFSWFSMLFGAGIGIGMLTYSTGEPLAHFQNNPDIIRGLVEPASAA
AVPSAYEYTFLHWGLSAWATYALVGLAVGYVAYRRNLPLTIRSSLAPIFGLRLSGNSGHLVDIVAVVATILGVSVTMGLG
VEQFVAGLYRLGFGEWLIKEGGTASDWAIVLALVLLVGASTISALSGVGRGIKWLSNLNMGLSFLLLAVFLVFGAGLYGL
QLLGEGLVDHVARLPERMLALWTADGTETGNALAQWQLDWSVFYWAWWIAFAPFVGMFIARISRGRTVREYVLGVILVPS
LMCFLWMALAGGTAIQLELDGTAQGAILSAGISDQIYATITALMSPGVAYLLSGLIVILLMTYLITSADSAILIVNTING
AGENDGSRNRHILFWGAALALVVGSMLILGGIDAIRITMIIGALPFSVVVALMAISIVKAIVYDVIRKRHGVQTHCEDIL
AEAAARAE

Sequences:

>Translated_568_residues
MTSDTDTAVSQPAIDPPLCELPVATAERGFYTGFSTHVAIPAKIVVSVLIAWAIFFPTHASDTLAIANQTIIRQFAFWYV
YLVAFLVVVCLVLALVPQSGSLRIGAPGEKPEFSRFSWFSMLFGAGIGIGMLTYSTGEPLAHFQNNPDIIRGLVEPASAA
AVPSAYEYTFLHWGLSAWATYALVGLAVGYVAYRRNLPLTIRSSLAPIFGLRLSGNSGHLVDIVAVVATILGVSVTMGLG
VEQFVAGLYRLGFGEWLIKEGGTASDWAIVLALVLLVGASTISALSGVGRGIKWLSNLNMGLSFLLLAVFLVFGAGLYGL
QLLGEGLVDHVARLPERMLALWTADGTETGNALAQWQLDWSVFYWAWWIAFAPFVGMFIARISRGRTVREYVLGVILVPS
LMCFLWMALAGGTAIQLELDGTAQGAILSAGISDQIYATITALMSPGVAYLLSGLIVILLMTYLITSADSAILIVNTING
AGENDGSRNRHILFWGAALALVVGSMLILGGIDAIRITMIIGALPFSVVVALMAISIVKAIVYDVIRKRHGVQTHCEDIL
AEAAARAE
>Mature_567_residues
TSDTDTAVSQPAIDPPLCELPVATAERGFYTGFSTHVAIPAKIVVSVLIAWAIFFPTHASDTLAIANQTIIRQFAFWYVY
LVAFLVVVCLVLALVPQSGSLRIGAPGEKPEFSRFSWFSMLFGAGIGIGMLTYSTGEPLAHFQNNPDIIRGLVEPASAAA
VPSAYEYTFLHWGLSAWATYALVGLAVGYVAYRRNLPLTIRSSLAPIFGLRLSGNSGHLVDIVAVVATILGVSVTMGLGV
EQFVAGLYRLGFGEWLIKEGGTASDWAIVLALVLLVGASTISALSGVGRGIKWLSNLNMGLSFLLLAVFLVFGAGLYGLQ
LLGEGLVDHVARLPERMLALWTADGTETGNALAQWQLDWSVFYWAWWIAFAPFVGMFIARISRGRTVREYVLGVILVPSL
MCFLWMALAGGTAIQLELDGTAQGAILSAGISDQIYATITALMSPGVAYLLSGLIVILLMTYLITSADSAILIVNTINGA
GENDGSRNRHILFWGAALALVVGSMLILGGIDAIRITMIIGALPFSVVVALMAISIVKAIVYDVIRKRHGVQTHCEDILA
EAAARAE

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=474, Percent_Identity=36.0759493670886, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI1788102, Length=483, Percent_Identity=26.5010351966874, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1786224, Length=443, Percent_Identity=28.2167042889391, Blast_Score=122, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 60660; Mature: 60529

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSDTDTAVSQPAIDPPLCELPVATAERGFYTGFSTHVAIPAKIVVSVLIAWAIFFPTHA
CCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
SDTLAIANQTIIRQFAFWYVYLVAFLVVVCLVLALVPQSGSLRIGAPGEKPEFSRFSWFS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHH
MLFGAGIGIGMLTYSTGEPLAHFQNNPDIIRGLVEPASAAAVPSAYEYTFLHWGLSAWAT
HHHHCCCCHHHEEECCCCCHHHCCCCHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHH
YALVGLAVGYVAYRRNLPLTIRSSLAPIFGLRLSGNSGHLVDIVAVVATILGVSVTMGLG
HHHHHHHHHHHHHHCCCCEEEHHHCCCHHEEEECCCCCCHHHHHHHHHHHHHHHHHHCCC
VEQFVAGLYRLGFGEWLIKEGGTASDWAIVLALVLLVGASTISALSGVGRGIKWLSNLNM
HHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GLSFLLLAVFLVFGAGLYGLQLLGEGLVDHVARLPERMLALWTADGTETGNALAQWQLDW
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECH
SVFYWAWWIAFAPFVGMFIARISRGRTVREYVLGVILVPSLMCFLWMALAGGTAIQLELD
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
GTAQGAILSAGISDQIYATITALMSPGVAYLLSGLIVILLMTYLITSADSAILIVNTING
CCCCCCEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
AGENDGSRNRHILFWGAALALVVGSMLILGGIDAIRITMIIGALPFSVVVALMAISIVKA
CCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVYDVIRKRHGVQTHCEDILAEAAARAE
HHHHHHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSDTDTAVSQPAIDPPLCELPVATAERGFYTGFSTHVAIPAKIVVSVLIAWAIFFPTHA
CCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
SDTLAIANQTIIRQFAFWYVYLVAFLVVVCLVLALVPQSGSLRIGAPGEKPEFSRFSWFS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHH
MLFGAGIGIGMLTYSTGEPLAHFQNNPDIIRGLVEPASAAAVPSAYEYTFLHWGLSAWAT
HHHHCCCCHHHEEECCCCCHHHCCCCHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHH
YALVGLAVGYVAYRRNLPLTIRSSLAPIFGLRLSGNSGHLVDIVAVVATILGVSVTMGLG
HHHHHHHHHHHHHHCCCCEEEHHHCCCHHEEEECCCCCCHHHHHHHHHHHHHHHHHHCCC
VEQFVAGLYRLGFGEWLIKEGGTASDWAIVLALVLLVGASTISALSGVGRGIKWLSNLNM
HHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GLSFLLLAVFLVFGAGLYGLQLLGEGLVDHVARLPERMLALWTADGTETGNALAQWQLDW
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECH
SVFYWAWWIAFAPFVGMFIARISRGRTVREYVLGVILVPSLMCFLWMALAGGTAIQLELD
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
GTAQGAILSAGISDQIYATITALMSPGVAYLLSGLIVILLMTYLITSADSAILIVNTING
CCCCCCEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
AGENDGSRNRHILFWGAALALVVGSMLILGGIDAIRITMIIGALPFSVVVALMAISIVKA
CCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVYDVIRKRHGVQTHCEDILAEAAARAE
HHHHHHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]