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The map label for this gene is lipB

Identifier: 85372909

GI number: 85372909

Start: 108361

End: 109020

Strand: Direct

Name: lipB

Synonym: ELI_00410

Alternate gene names: 85372909

Gene position: 108361-109020 (Clockwise)

Preceding gene: 85372907

Following gene: 85372910

Centisome position: 3.55

GC content: 64.85

Gene sequence:

>660_bases
ATGAGTGATCCCCTGTCCGATATCGAATGGCGCGTGGAACCGGGCCAGGTGCAGTATCGCACCGCGCTCGACGCGATGGA
CGCGCGCAATGCTGCGATCGCGGCGGGTGACGCGCGTGAACTGATCTGGCTGCTCGAACATCCGCCCGTTTACACCGCCG
GGACCAGCGCCAATGCGGCGGAGCTTCTCGACCCGCGCTTCGAAGTGGTGGAAGCCGGACGGGGCGGACGCTACACCTAT
CACGGGCCGGGCCAGCGGGTCGGCTATGCACTGCTGGATTTGCGCAAACGCGGCAAGGATGTGCGCCGCTTCGTCCATGC
CATCGAGGACTGGGTGATTGCCACCCTGCGCACTTTCGGCGTCGAAAGCTGGTCGGTCGATGGCCGTGTCGGCATCTGGA
CGAAGGATATCGACGGTGCGGAAGCCAAGATCGGCGCCATAGGCGTGCGCGTGCGGCGATGGGTCACCATGCACGGCTTC
TCGGTCAATCTCGATCCGGACCTGTCGCATTTTGCCGGCATCGTCCCTTGCGGGATCGACGAATTCGGGGTCACCAGCCT
TGCCAGGCTCGGCATTTCGCTTGCGCCGGAGGAATGGGATGCGGCATTGCAGGGCCGGGCCGGGCAGTTTCTCGCCCAAC
TCGAAGGAGAGCCCCTGTGA

Upstream 100 bases:

>100_bases
GTCCCACGCGCGGTTCATGTCGAATTTCATCTGTGGCTCTCCCCGTATGGGCCGTTTGTCACTTGATAACCGCGAGTGCA
CGCGCCACGCAATGTCTCAA

Downstream 100 bases:

>100_bases
AGCGAATTTCGATTGCCATGGTGGCGCTGGCCTTTCCGCTTGCCGGTTGCGGCGACGATCCCGCCCCGGTCGCCGAGACG
ACCGACGATGCCGGTGCGCA

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MSDPLSDIEWRVEPGQVQYRTALDAMDARNAAIAAGDARELIWLLEHPPVYTAGTSANAAELLDPRFEVVEAGRGGRYTY
HGPGQRVGYALLDLRKRGKDVRRFVHAIEDWVIATLRTFGVESWSVDGRVGIWTKDIDGAEAKIGAIGVRVRRWVTMHGF
SVNLDPDLSHFAGIVPCGIDEFGVTSLARLGISLAPEEWDAALQGRAGQFLAQLEGEPL

Sequences:

>Translated_219_residues
MSDPLSDIEWRVEPGQVQYRTALDAMDARNAAIAAGDARELIWLLEHPPVYTAGTSANAAELLDPRFEVVEAGRGGRYTY
HGPGQRVGYALLDLRKRGKDVRRFVHAIEDWVIATLRTFGVESWSVDGRVGIWTKDIDGAEAKIGAIGVRVRRWVTMHGF
SVNLDPDLSHFAGIVPCGIDEFGVTSLARLGISLAPEEWDAALQGRAGQFLAQLEGEPL
>Mature_218_residues
SDPLSDIEWRVEPGQVQYRTALDAMDARNAAIAAGDARELIWLLEHPPVYTAGTSANAAELLDPRFEVVEAGRGGRYTYH
GPGQRVGYALLDLRKRGKDVRRFVHAIEDWVIATLRTFGVESWSVDGRVGIWTKDIDGAEAKIGAIGVRVRRWVTMHGFS
VNLDPDLSHFAGIVPCGIDEFGVTSLARLGISLAPEEWDAALQGRAGQFLAQLEGEPL

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=152, Percent_Identity=39.4736842105263, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI87081767, Length=138, Percent_Identity=41.304347826087, Blast_Score=114, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6323268, Length=216, Percent_Identity=25.9259259259259, Blast_Score=81, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355483, Length=172, Percent_Identity=37.2093023255814, Blast_Score=95, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_ERYLH (Q2NDR7)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_456971.1
- HSSP:   Q10404
- ProteinModelPortal:   Q2NDR7
- SMR:   Q2NDR7
- STRING:   Q2NDR7
- GeneID:   3870822
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_00410
- NMPDR:   fig|314225.3.peg.3015
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   DLKRRRP
- PhylomeDB:   Q2NDR7
- ProtClustDB:   PRK14341
- BioCyc:   ELIT314225:ELI_00410-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 24007; Mature: 23876

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 177-177

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPLSDIEWRVEPGQVQYRTALDAMDARNAAIAAGDARELIWLLEHPPVYTAGTSANAA
CCCCCCCCEEEECCCCEEHHHHHHHHHCCCCEEECCCHHHEEEEECCCCEEECCCCCCHH
ELLDPRFEVVEAGRGGRYTYHGPGQRVGYALLDLRKRGKDVRRFVHAIEDWVIATLRTFG
HHHCCHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
VESWSVDGRVGIWTKDIDGAEAKIGAIGVRVRRWVTMHGFSVNLDPDLSHFAGIVPCGID
CCCCCCCCEEEEEECCCCCCCHHHHHHHHEEEEEEEECCEEEECCCCHHHHCCCCCCCCC
EFGVTSLARLGISLAPEEWDAALQGRAGQFLAQLEGEPL
HHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHCCCCCC
>Mature Secondary Structure 
SDPLSDIEWRVEPGQVQYRTALDAMDARNAAIAAGDARELIWLLEHPPVYTAGTSANAA
CCCCCCCEEEECCCCEEHHHHHHHHHCCCCEEECCCHHHEEEEECCCCEEECCCCCCHH
ELLDPRFEVVEAGRGGRYTYHGPGQRVGYALLDLRKRGKDVRRFVHAIEDWVIATLRTFG
HHHCCHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
VESWSVDGRVGIWTKDIDGAEAKIGAIGVRVRRWVTMHGFSVNLDPDLSHFAGIVPCGID
CCCCCCCCEEEEEECCCCCCCHHHHHHHHEEEEEEEECCEEEECCCCHHHHCCCCCCCCC
EFGVTSLARLGISLAPEEWDAALQGRAGQFLAQLEGEPL
HHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA