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The map label for this gene is intB [H]

Identifier: 85372848

GI number: 85372848

Start: 33987

End: 35177

Strand: Direct

Name: intB [H]

Synonym: ELI_00105

Alternate gene names: 85372848

Gene position: 33987-35177 (Clockwise)

Preceding gene: 85372847

Following gene: 85372852

Centisome position: 1.11

GC content: 60.12

Gene sequence:

>1191_bases
GTGGGCACGATTGTTGGCACTGGAGCGATCATCATGGGCAAACTGACGGCGGTAAAAGCTAAAGCGATCAAAGAGGCGGG
GCGCTATAGCGATGGGAACGGGCTTCAACTCGTGGTCAAAGCCAGCGGTGCCAAATCCTGGATTCTACGAGTTCAGCACG
ATGGCAAGAGGCGCGATATCGGATTGGGGCCTCTGTCCGAAGTTGGACCCGCAGAGGCCCGTGAGAAGGCCGTGGAGGCA
CGGAAGGTAATTCGTGGGGGTGGTGACCCGTCGGGCTTGAAAAGCGGCTCAGGAAGAATCTCTGGCCGTCCTCCGACCTT
TCGCGAGCTGGCGACCAGCTACCATGACGACCACAAGAGTAGCTGGAAAAACGCGAAACATCGCGAACAGTGGCTTTCCA
CTCTCGACACCTACGCCTTTCCAATCTTCGGCGACCAGCCGGTCGATCGTGTGGACAGTCGAGACGTTCTCGATTGCCTC
AAGCCGATCTGGAACGCGAAACCGGAGACGGCTCGCCGTGTCCTACAACGTGTTCTTAGAGTTCTGAATTACGCTCATGG
GATGGGGCATCGGGAGCACGAAGCGCCAACGCAGTCCATACGGGCGGCTTTGGGCAAGCAGCGTTCTCAAGTGAGGCACC
ATGCGTCGTTGCCCTACGCCGAAATGCCGGAGCTGATGGCAAAGCTAGAGGCCGCATCGGGCGTTGGCGCAATGGCGCTT
CGCTTCGCGATCCTGACTGCCGCGCGATCTGGCGAGGTTCGTGGCGCTCAGTGGAACGAGATGGATTTGGCAAAGAAAAC
TTGGACGGTTCCGGCAGAGCGGATGAAGGCTCGCCGCGCACACGCTGTCCCGCTATCGCCAGCCGCGCTCGCCATCTTGG
AGGATGCGAAGGGGCTAAGGAAGAAGCCCGAGGGCTTCGTGTTCCCTAGCAGTCGCGGCATCGCGCTCAGCGACATGACG
TTGCTGAAGGCCATCCGCGCTGCGAGCGATCAGAACACCACAGTGCATGGCTGTCGCACCAGCTTTCGTGTGTGGGCTGC
GGAGAAGACCAGCTGTCCAGCCGCCGCTGCCGAGGCAGCGCTTGCGCATACGATACACAACAAGGCTGAAGCGGCTTATC
ATCGAACCGATTATCTCGACATACGGCGGCAGCTCATGGCCGATTGGGGGGCGTTCCTTGCTGGCGGGTGA

Upstream 100 bases:

>100_bases
GATGATCGTGGGTGATCGCGTTAGCTATGGATTTCCTTGTGTTCTTACTTGGTAAAGTTCGCCCACGTGCATTGACGTTC
AGCTCCAACCGTTCAAAAGT

Downstream 100 bases:

>100_bases
TCAGTTCGCTCCCTATGGCATTCAATTTTTGCCTCGTTACTAGGTAACAGTTATTTTGTGTTGTAAATTCAACAGGTTAT
ATGACCTTTATATATTCATT

Product: site-specfic recombinase, phage integrase family protein

Products: NA

Alternate protein names: Int(P4) [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MGTIVGTGAIIMGKLTAVKAKAIKEAGRYSDGNGLQLVVKASGAKSWILRVQHDGKRRDIGLGPLSEVGPAEAREKAVEA
RKVIRGGGDPSGLKSGSGRISGRPPTFRELATSYHDDHKSSWKNAKHREQWLSTLDTYAFPIFGDQPVDRVDSRDVLDCL
KPIWNAKPETARRVLQRVLRVLNYAHGMGHREHEAPTQSIRAALGKQRSQVRHHASLPYAEMPELMAKLEAASGVGAMAL
RFAILTAARSGEVRGAQWNEMDLAKKTWTVPAERMKARRAHAVPLSPAALAILEDAKGLRKKPEGFVFPSSRGIALSDMT
LLKAIRAASDQNTTVHGCRTSFRVWAAEKTSCPAAAAEAALAHTIHNKAEAAYHRTDYLDIRRQLMADWGAFLAGG

Sequences:

>Translated_396_residues
MGTIVGTGAIIMGKLTAVKAKAIKEAGRYSDGNGLQLVVKASGAKSWILRVQHDGKRRDIGLGPLSEVGPAEAREKAVEA
RKVIRGGGDPSGLKSGSGRISGRPPTFRELATSYHDDHKSSWKNAKHREQWLSTLDTYAFPIFGDQPVDRVDSRDVLDCL
KPIWNAKPETARRVLQRVLRVLNYAHGMGHREHEAPTQSIRAALGKQRSQVRHHASLPYAEMPELMAKLEAASGVGAMAL
RFAILTAARSGEVRGAQWNEMDLAKKTWTVPAERMKARRAHAVPLSPAALAILEDAKGLRKKPEGFVFPSSRGIALSDMT
LLKAIRAASDQNTTVHGCRTSFRVWAAEKTSCPAAAAEAALAHTIHNKAEAAYHRTDYLDIRRQLMADWGAFLAGG
>Mature_395_residues
GTIVGTGAIIMGKLTAVKAKAIKEAGRYSDGNGLQLVVKASGAKSWILRVQHDGKRRDIGLGPLSEVGPAEAREKAVEAR
KVIRGGGDPSGLKSGSGRISGRPPTFRELATSYHDDHKSSWKNAKHREQWLSTLDTYAFPIFGDQPVDRVDSRDVLDCLK
PIWNAKPETARRVLQRVLRVLNYAHGMGHREHEAPTQSIRAALGKQRSQVRHHASLPYAEMPELMAKLEAASGVGAMALR
FAILTAARSGEVRGAQWNEMDLAKKTWTVPAERMKARRAHAVPLSPAALAILEDAKGLRKKPEGFVFPSSRGIALSDMTL
LKAIRAASDQNTTVHGCRTSFRVWAAEKTSCPAAAAEAALAHTIHNKAEAAYHRTDYLDIRRQLMADWGAFLAGG

Specific function: Unknown

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=389, Percent_Identity=30.8483290488432, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI145693166, Length=374, Percent_Identity=30.7486631016043, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1788690, Length=380, Percent_Identity=27.3684210526316, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 43093; Mature: 42962

Theoretical pI: Translated: 10.67; Mature: 10.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTIVGTGAIIMGKLTAVKAKAIKEAGRYSDGNGLQLVVKASGAKSWILRVQHDGKRRDI
CCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCCC
GLGPLSEVGPAEAREKAVEARKVIRGGGDPSGLKSGSGRISGRPPTFRELATSYHDDHKS
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
SWKNAKHREQWLSTLDTYAFPIFGDQPVDRVDSRDVLDCLKPIWNAKPETARRVLQRVLR
HHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
VLNYAHGMGHREHEAPTQSIRAALGKQRSQVRHHASLPYAEMPELMAKLEAASGVGAMAL
HHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHH
RFAILTAARSGEVRGAQWNEMDLAKKTWTVPAERMKARRAHAVPLSPAALAILEDAKGLR
HHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
KKPEGFVFPSSRGIALSDMTLLKAIRAASDQNTTVHGCRTSFRVWAAEKTSCPAAAAEAA
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCEEEEECCCCCCCHHHHHHH
LAHTIHNKAEAAYHRTDYLDIRRQLMADWGAFLAGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GTIVGTGAIIMGKLTAVKAKAIKEAGRYSDGNGLQLVVKASGAKSWILRVQHDGKRRDI
CCEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCCC
GLGPLSEVGPAEAREKAVEARKVIRGGGDPSGLKSGSGRISGRPPTFRELATSYHDDHKS
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
SWKNAKHREQWLSTLDTYAFPIFGDQPVDRVDSRDVLDCLKPIWNAKPETARRVLQRVLR
HHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
VLNYAHGMGHREHEAPTQSIRAALGKQRSQVRHHASLPYAEMPELMAKLEAASGVGAMAL
HHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHH
RFAILTAARSGEVRGAQWNEMDLAKKTWTVPAERMKARRAHAVPLSPAALAILEDAKGLR
HHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
KKPEGFVFPSSRGIALSDMTLLKAIRAASDQNTTVHGCRTSFRVWAAEKTSCPAAAAEAA
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCEEEEECCCCCCCHHHHHHH
LAHTIHNKAEAAYHRTDYLDIRRQLMADWGAFLAGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]