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The map label for this gene is fadA [H]

Identifier: 85372836

GI number: 85372836

Start: 18224

End: 19492

Strand: Reverse

Name: fadA [H]

Synonym: ELI_00045

Alternate gene names: 85372836

Gene position: 19492-18224 (Counterclockwise)

Preceding gene: 85372843

Following gene: 85372835

Centisome position: 0.64

GC content: 64.78

Gene sequence:

>1269_bases
ATGCCAGAAGCCTATATCGTGGACGCCGTTCGCACCCCGCGCGGGATCGGGAAGGTCGGCAAGGGCGCGCTCGCAAACGA
GCATCCGCAGCATCTCGCCGCGACCGTCCTGAAAGCCATCAAGGAGCGCAACGATCTCGACACGGCGACGGTCGACGACG
TGATCTGGTCGGTCAGCACGCAGGACGGCAAGCAGGCGGGCGATATGGGTCGCATGGCCGCGCTCGATGCAGGCTACGAC
GTGACCAGCAGCGGCATGACGCTCGACCGCTTCTGCGGCGGCGGCATCACCTCGGTGAACCTGGCCGCAGCGCAAGTCAT
GTCCGGCATGGCCGATTGCGTGGTCGCCGGCGGGACGGAGATGATGAGCCTCACCGCCGAGATGGCCAAGGCCAAGATGC
AGGCGGGCATCGATCTGCCCCGCATGGGCAGCGGCAATGCACGCCTGCAGGCGACGCATCCGCAGAGCCACCAGGGGGTG
TGCGGCGATGCCATCGCCAGCATGGAAGGTTTCACCCGCGAAGAGCTCGACGAAGTCGGCTATCGCAGCCAACAACGCGC
GGCGCAGGCGATCGAGGAAGGCCGCTTCGACAAGTCGATCGTTCCGGTAGTCGACGATGACGGCAACGTGATCCTCGACC
GGGAGGAATTCCCGCGTCCGCAGACGACGATGGAAACGCTGGGACAACTCGAACCCGCTTTCACCAAGCTCGCGGACATT
CCGCTCGACAAGAAAGGCACGACCTTCCGCGGCCTTATCAACCAGAAGTATCCCGACCTCGAGATCAAGCACTTTCACCA
TGCCGGTAACAGCTCGGGCGTGGTCGATGGCGCGGCGGGCGTGCTGGTGGCGAGCAAGGAATATTGCGAAAAGCACGGCC
TCAAGCCGCGCGCGCGCATCGTCGCCACCGCCAATATGGGCGACGACCCGACGCTGATGCTCAACGCGCCGGTCCCGGCG
GCCAAGAAGGTGCTCGAGAAAGCGGGCATGACGCTCGACGATATCGACCTGTTCGAAATCAACGAGGCCTTCGCCGTGGT
CGCCGCCAAGTTCGTGCGCGATCTCGATCTCGACTGGGACAAGGTCAACGTCAATGGCGGCGCCATCGCGCTCGGCCACC
CGATCGGCGCGACCGGCTCGATGCTGATCGGCACCATGGTCGACGAGCTCGAACGGCAAGGTAAGACAACCGGCATGGTT
ACCATGTGCGCCGCCGGCGGCATGGCCCCGGCGATCATCGTCGAGCGGGTCGACGATTTCGTCGACTAG

Upstream 100 bases:

>100_bases
ATGGCAAATTGAAACTTTTCTTGTGCCGGTGCTGGCCAAGCTTTACGAAAACGTCAACCGGACTCTTGGTCCGGCACAAT
GATTCTTTGGAGAGGATAGC

Downstream 100 bases:

>100_bases
TCGCTTCGCCATTACGAATCGAATAGCGAATATCTTCGGGGCGGCCCGATGGGTCGCCCCTTTTCGTCCCGGCTAGTCCC
GGGCAGAGGCTTCTCTCAAG

Product: acetyl-CoA acetyltransferase

Products: CoA; 3-oxoacyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MPEAYIVDAVRTPRGIGKVGKGALANEHPQHLAATVLKAIKERNDLDTATVDDVIWSVSTQDGKQAGDMGRMAALDAGYD
VTSSGMTLDRFCGGGITSVNLAAAQVMSGMADCVVAGGTEMMSLTAEMAKAKMQAGIDLPRMGSGNARLQATHPQSHQGV
CGDAIASMEGFTREELDEVGYRSQQRAAQAIEEGRFDKSIVPVVDDDGNVILDREEFPRPQTTMETLGQLEPAFTKLADI
PLDKKGTTFRGLINQKYPDLEIKHFHHAGNSSGVVDGAAGVLVASKEYCEKHGLKPRARIVATANMGDDPTLMLNAPVPA
AKKVLEKAGMTLDDIDLFEINEAFAVVAAKFVRDLDLDWDKVNVNGGAIALGHPIGATGSMLIGTMVDELERQGKTTGMV
TMCAAGGMAPAIIVERVDDFVD

Sequences:

>Translated_422_residues
MPEAYIVDAVRTPRGIGKVGKGALANEHPQHLAATVLKAIKERNDLDTATVDDVIWSVSTQDGKQAGDMGRMAALDAGYD
VTSSGMTLDRFCGGGITSVNLAAAQVMSGMADCVVAGGTEMMSLTAEMAKAKMQAGIDLPRMGSGNARLQATHPQSHQGV
CGDAIASMEGFTREELDEVGYRSQQRAAQAIEEGRFDKSIVPVVDDDGNVILDREEFPRPQTTMETLGQLEPAFTKLADI
PLDKKGTTFRGLINQKYPDLEIKHFHHAGNSSGVVDGAAGVLVASKEYCEKHGLKPRARIVATANMGDDPTLMLNAPVPA
AKKVLEKAGMTLDDIDLFEINEAFAVVAAKFVRDLDLDWDKVNVNGGAIALGHPIGATGSMLIGTMVDELERQGKTTGMV
TMCAAGGMAPAIIVERVDDFVD
>Mature_421_residues
PEAYIVDAVRTPRGIGKVGKGALANEHPQHLAATVLKAIKERNDLDTATVDDVIWSVSTQDGKQAGDMGRMAALDAGYDV
TSSGMTLDRFCGGGITSVNLAAAQVMSGMADCVVAGGTEMMSLTAEMAKAKMQAGIDLPRMGSGNARLQATHPQSHQGVC
GDAIASMEGFTREELDEVGYRSQQRAAQAIEEGRFDKSIVPVVDDDGNVILDREEFPRPQTTMETLGQLEPAFTKLADIP
LDKKGTTFRGLINQKYPDLEIKHFHHAGNSSGVVDGAAGVLVASKEYCEKHGLKPRARIVATANMGDDPTLMLNAPVPAA
KKVLEKAGMTLDDIDLFEINEAFAVVAAKFVRDLDLDWDKVNVNGGAIALGHPIGATGSMLIGTMVDELERQGKTTGMVT
MCAAGGMAPAIIVERVDDFVD

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=418, Percent_Identity=35.4066985645933, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI167614485, Length=416, Percent_Identity=34.375, Blast_Score=206, Evalue=4e-53,
Organism=Homo sapiens, GI4501853, Length=431, Percent_Identity=33.8747099767981, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI4557237, Length=423, Percent_Identity=28.6052009456265, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI4504327, Length=448, Percent_Identity=29.9107142857143, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI194353979, Length=89, Percent_Identity=51.685393258427, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1788554, Length=428, Percent_Identity=39.4859813084112, Blast_Score=245, Evalue=3e-66,
Organism=Escherichia coli, GI1787663, Length=430, Percent_Identity=37.4418604651163, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI48994986, Length=425, Percent_Identity=34.3529411764706, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI87082165, Length=428, Percent_Identity=34.8130841121495, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI1788683, Length=430, Percent_Identity=26.046511627907, Blast_Score=108, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI133906874, Length=419, Percent_Identity=32.9355608591885, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI25147385, Length=424, Percent_Identity=31.6037735849057, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17535921, Length=429, Percent_Identity=27.2727272727273, Blast_Score=148, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17551802, Length=454, Percent_Identity=28.4140969162996, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17535917, Length=421, Percent_Identity=28.2660332541568, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6322031, Length=412, Percent_Identity=32.2815533980583, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6325229, Length=423, Percent_Identity=31.6784869976359, Blast_Score=152, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24655093, Length=427, Percent_Identity=34.4262295081967, Blast_Score=218, Evalue=6e-57,
Organism=Drosophila melanogaster, GI17648125, Length=428, Percent_Identity=34.5794392523364, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24640423, Length=425, Percent_Identity=31.7647058823529, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI17137578, Length=448, Percent_Identity=28.3482142857143, Blast_Score=145, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16

Molecular weight: Translated: 44772; Mature: 44640

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEAYIVDAVRTPRGIGKVGKGALANEHPQHLAATVLKAIKERNDLDTATVDDVIWSVST
CCCCEEEECHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
QDGKQAGDMGRMAALDAGYDVTSSGMTLDRFCGGGITSVNLAAAQVMSGMADCVVAGGTE
CCCCHHCCCHHHHHHCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHH
MMSLTAEMAKAKMQAGIDLPRMGSGNARLQATHPQSHQGVCGDAIASMEGFTREELDEVG
HHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHC
YRSQQRAAQAIEEGRFDKSIVPVVDDDGNVILDREEFPRPQTTMETLGQLEPAFTKLADI
CCHHHHHHHHHHHCCCCCCCCEEEECCCCEEECHHHCCCCHHHHHHHHCCCHHHHHHHCC
PLDKKGTTFRGLINQKYPDLEIKHFHHAGNSSGVVDGAAGVLVASKEYCEKHGLKPRARI
CCCCCCCHHHHHHCCCCCCCCEEHEECCCCCCCCCCCCCCEEEECHHHHHHCCCCCCCEE
VATANMGDDPTLMLNAPVPAAKKVLEKAGMTLDDIDLFEINEAFAVVAAKFVRDLDLDWD
EEECCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
KVNVNGGAIALGHPIGATGSMLIGTMVDELERQGKTTGMVTMCAAGGMAPAIIVERVDDF
EEECCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHH
VD
CC
>Mature Secondary Structure 
PEAYIVDAVRTPRGIGKVGKGALANEHPQHLAATVLKAIKERNDLDTATVDDVIWSVST
CCCEEEECHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
QDGKQAGDMGRMAALDAGYDVTSSGMTLDRFCGGGITSVNLAAAQVMSGMADCVVAGGTE
CCCCHHCCCHHHHHHCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHH
MMSLTAEMAKAKMQAGIDLPRMGSGNARLQATHPQSHQGVCGDAIASMEGFTREELDEVG
HHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHC
YRSQQRAAQAIEEGRFDKSIVPVVDDDGNVILDREEFPRPQTTMETLGQLEPAFTKLADI
CCHHHHHHHHHHHCCCCCCCCEEEECCCCEEECHHHCCCCHHHHHHHHCCCHHHHHHHCC
PLDKKGTTFRGLINQKYPDLEIKHFHHAGNSSGVVDGAAGVLVASKEYCEKHGLKPRARI
CCCCCCCHHHHHHCCCCCCCCEEHEECCCCCCCCCCCCCCEEEECHHHHHHCCCCCCCEE
VATANMGDDPTLMLNAPVPAAKKVLEKAGMTLDDIDLFEINEAFAVVAAKFVRDLDLDWD
EEECCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
KVNVNGGAIALGHPIGATGSMLIGTMVDELERQGKTTGMVTMCAAGGMAPAIIVERVDDF
EEECCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHH
VD
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acetyl-CoA

Specific reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]