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The map label for this gene is ilvG [H]

Identifier: 85060375

GI number: 85060375

Start: 4105812

End: 4107458

Strand: Reverse

Name: ilvG [H]

Synonym: SG2397

Alternate gene names: 85060375

Gene position: 4107458-4105812 (Counterclockwise)

Preceding gene: 85060376

Following gene: 85060374

Centisome position: 98.47

GC content: 59.44

Gene sequence:

>1647_bases
ATGAATGGAGCTCAATGGGTAATACAGGCGCTGCGGGCACAGGGTGTGGACATGGTTTTCGGCTACCCCGGCGGCGCTAT
CATGCCGGTATACGACGCGCTATTCGACGGCGGGGTGGAACACCTGCTATGCCGTCATGAGCAGGGCGCGGCCATCGCAG
CAATCGGCTACGCACGGGCTACCGGTAAAGTGGGCGTGTGCATCGCTACCTCCGGCCCTGGCGCCACCAACCTTATTACC
GGGCTGGCCGATGCTTTATTGGATTCGGTGCCGGTTGTGGCGATCACTGGGCAGGTCGGGTCGGCGTTCATCGGCACCGA
CGCTTTTCAAGAGATTGATGTACTGGGCCTGTCGCTCGCCTGTACGAAACATAGCTTTCTGGTGGAGTCGCTGGACGCGC
TGCCAGGCATCATCACCGAAGCCTTCGCTATCGCCACCAGCGGGCGCACGGGACCCGTTTTGATTGATATTCCCAAAGAT
ATTCAATTGGCGGACGGCGATCTGACGCCGCATTTACTGAGTCTCGACGATCAGGCCAGCAGCATTCAGGGCGATCTGGA
TGGCGCGCGTCGGCTGATGCGCCAGGCGACACGCCCGATTCTCTATGTCGGCGGCGGCGTCGGCATGGCCGGTGCGGTTC
CGGCGTTGCGGCAATTGATTACCCAAACCGGCCTTCCCGCCGTGGCGACGCTAAAAGGTCTGGGTGCGCCGGATGTCGAT
GATGCTTGTTATTTAGGCATGCTGGGTATGCATGGCAATCAGGCCGCCAATCTGGCGGTACAGGCCTGCGATTTGCTGAT
CGCGGTTGGCGCGCGTTTCGACGATCGGGTGACCGGCCGGCTGAATGCGTTTGCTCCCCATGCCAAGGTGATTCATCTGG
ATATAGATCCTGCCGAGTTGGGCAAGTTGCGCCAGGCGCACATCGCGCTGCTGGGAGATTTGAATCATCTGCTGCCGGCG
CTGGCGCAACCGTTGTCTATCGACGCCTGGCGCGTTGAGACCCAGGCGCTGAAAGCGGAGTACCGCTGGCGCTATGACAA
TCCCGGCGCGGGGATTTACGCCCCGGCGCTGCTGCGGGCCATCAGCGATCGCGCCCCCGACGAGGCTGTGGTGACCACTG
ATGTCGGGCAACATCAAATGTGGGCCGCCCAGCATATGCGTTTCAGCCGGCCGGAGAATTTCATCACCTCCAGCGGCCTG
GGGACTATGGGATTCGGCATTCCCGCCGCGATAGGAGCGCAAATTGCGCGGCTGGAGGATACCGTCATCTGCATCTCCGG
CGACGGCTCCTTCATGATGAATGTGCAGGAGCTGACCACGATTAAGCGAAAACGGTTACCGCTGAAGATCGTGCTGCTGG
ATAATCAGCGCTTAGGCATGGTCCGTCAATGGCAGCAATTGTTCTTCGATAAACGTTATAGCGAAACCACCCTGACCGAT
AACCCCGATTTTCTCACGCTGGCCAGCGCCTTCAATATTCCCGGCCTGCATATCACCCGTAAAGACCAAATCCCGCAGGC
GCTTGACTCCCTGTTTAACCAACCAGGACCGTTCCTGCTGCATGTCTCTATCGATGAGCTTGAGAATGTCTGGCCCCTGG
TGCCTCCCGGCGCCGGTAATGAAACCATGCTGGAGAAATCATTATGA

Upstream 100 bases:

>100_bases
TCGGGGGTTTTTTTTGGGGCATCAATCAAGCGACAGGAGAACACTCAATGATAATTAGAATATTGTTCTGTCCCTGCGGC
GCGAAGGCGGGGAACTAACC

Downstream 100 bases:

>100_bases
TGCAACATTCCCTTTCCATTCAGGCGCGCTTTCGCCCGGAAGTCGTGGAACGTATATTGCGGGTTGTCCGCCACCGCGGT
TTTGAACTGTGCGCCTTAAA

Product: acetolactate synthase 2 catalytic subunit

Products: NA

Alternate protein names: AHAS-II; ALS-II; Acetohydroxy-acid synthase II large subunit [H]

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MNGAQWVIQALRAQGVDMVFGYPGGAIMPVYDALFDGGVEHLLCRHEQGAAIAAIGYARATGKVGVCIATSGPGATNLIT
GLADALLDSVPVVAITGQVGSAFIGTDAFQEIDVLGLSLACTKHSFLVESLDALPGIITEAFAIATSGRTGPVLIDIPKD
IQLADGDLTPHLLSLDDQASSIQGDLDGARRLMRQATRPILYVGGGVGMAGAVPALRQLITQTGLPAVATLKGLGAPDVD
DACYLGMLGMHGNQAANLAVQACDLLIAVGARFDDRVTGRLNAFAPHAKVIHLDIDPAELGKLRQAHIALLGDLNHLLPA
LAQPLSIDAWRVETQALKAEYRWRYDNPGAGIYAPALLRAISDRAPDEAVVTTDVGQHQMWAAQHMRFSRPENFITSSGL
GTMGFGIPAAIGAQIARLEDTVICISGDGSFMMNVQELTTIKRKRLPLKIVLLDNQRLGMVRQWQQLFFDKRYSETTLTD
NPDFLTLASAFNIPGLHITRKDQIPQALDSLFNQPGPFLLHVSIDELENVWPLVPPGAGNETMLEKSL

Sequences:

>Translated_548_residues
MNGAQWVIQALRAQGVDMVFGYPGGAIMPVYDALFDGGVEHLLCRHEQGAAIAAIGYARATGKVGVCIATSGPGATNLIT
GLADALLDSVPVVAITGQVGSAFIGTDAFQEIDVLGLSLACTKHSFLVESLDALPGIITEAFAIATSGRTGPVLIDIPKD
IQLADGDLTPHLLSLDDQASSIQGDLDGARRLMRQATRPILYVGGGVGMAGAVPALRQLITQTGLPAVATLKGLGAPDVD
DACYLGMLGMHGNQAANLAVQACDLLIAVGARFDDRVTGRLNAFAPHAKVIHLDIDPAELGKLRQAHIALLGDLNHLLPA
LAQPLSIDAWRVETQALKAEYRWRYDNPGAGIYAPALLRAISDRAPDEAVVTTDVGQHQMWAAQHMRFSRPENFITSSGL
GTMGFGIPAAIGAQIARLEDTVICISGDGSFMMNVQELTTIKRKRLPLKIVLLDNQRLGMVRQWQQLFFDKRYSETTLTD
NPDFLTLASAFNIPGLHITRKDQIPQALDSLFNQPGPFLLHVSIDELENVWPLVPPGAGNETMLEKSL
>Mature_548_residues
MNGAQWVIQALRAQGVDMVFGYPGGAIMPVYDALFDGGVEHLLCRHEQGAAIAAIGYARATGKVGVCIATSGPGATNLIT
GLADALLDSVPVVAITGQVGSAFIGTDAFQEIDVLGLSLACTKHSFLVESLDALPGIITEAFAIATSGRTGPVLIDIPKD
IQLADGDLTPHLLSLDDQASSIQGDLDGARRLMRQATRPILYVGGGVGMAGAVPALRQLITQTGLPAVATLKGLGAPDVD
DACYLGMLGMHGNQAANLAVQACDLLIAVGARFDDRVTGRLNAFAPHAKVIHLDIDPAELGKLRQAHIALLGDLNHLLPA
LAQPLSIDAWRVETQALKAEYRWRYDNPGAGIYAPALLRAISDRAPDEAVVTTDVGQHQMWAAQHMRFSRPENFITSSGL
GTMGFGIPAAIGAQIARLEDTVICISGDGSFMMNVQELTTIKRKRLPLKIVLLDNQRLGMVRQWQQLFFDKRYSETTLTD
NPDFLTLASAFNIPGLHITRKDQIPQALDSLFNQPGPFLLHVSIDELENVWPLVPPGAGNETMLEKSL

Specific function: Catalyzes the first step in the biosynthesis of branched-chain amino acids [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=564, Percent_Identity=23.936170212766, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI21361361, Length=501, Percent_Identity=24.1516966067864, Blast_Score=119, Evalue=7e-27,
Organism=Escherichia coli, GI1790104, Length=551, Percent_Identity=44.6460980036298, Blast_Score=467, Evalue=1e-133,
Organism=Escherichia coli, GI87081685, Length=565, Percent_Identity=41.2389380530973, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1786717, Length=560, Percent_Identity=28.5714285714286, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI1787096, Length=541, Percent_Identity=28.4658040665434, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1788716, Length=570, Percent_Identity=24.5614035087719, Blast_Score=124, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17531299, Length=480, Percent_Identity=28.5416666666667, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17531301, Length=480, Percent_Identity=28.75, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17542570, Length=506, Percent_Identity=25.6916996047431, Blast_Score=115, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6323755, Length=570, Percent_Identity=43.859649122807, Blast_Score=446, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6320588, Length=572, Percent_Identity=21.1538461538462, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320816, Length=482, Percent_Identity=23.6514522821577, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19922626, Length=542, Percent_Identity=25.2767527675277, Blast_Score=153, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 58444; Mature: 58444

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGAQWVIQALRAQGVDMVFGYPGGAIMPVYDALFDGGVEHLLCRHEQGAAIAAIGYARA
CCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHCCCCCCEEEEEEEEEC
TGKVGVCIATSGPGATNLITGLADALLDSVPVVAITGQVGSAFIGTDAFQEIDVLGLSLA
CCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEECCCHHHHHHHHHEEEEE
CTKHSFLVESLDALPGIITEAFAIATSGRTGPVLIDIPKDIQLADGDLTPHLLSLDDQAS
HHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEECCCCCEECCCCCCCEEEECCCCHH
SIQGDLDGARRLMRQATRPILYVGGGVGMAGAVPALRQLITQTGLPAVATLKGLGAPDVD
HHCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCC
DACYLGMLGMHGNQAANLAVQACDLLIAVGARFDDRVTGRLNAFAPHAKVIHLDIDPAEL
CHHHHHEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCHHHH
GKLRQAHIALLGDLNHLLPALAQPLSIDAWRVETQALKAEYRWRYDNPGAGIYAPALLRA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHEEEECCCCCCHHHHHHHHH
ISDRAPDEAVVTTDVGQHQMWAAQHMRFSRPENFITSSGLGTMGFGIPAAIGAQIARLED
HHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHEECC
TVICISGDGSFMMNVQELTTIKRKRLPLKIVLLDNQRLGMVRQWQQLFFDKRYSETTLTD
EEEEEECCCCEEEEHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCC
NPDFLTLASAFNIPGLHITRKDQIPQALDSLFNQPGPFLLHVSIDELENVWPLVPPGAGN
CCCEEEEEHHCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCCCCCCCCCC
ETMLEKSL
CCHHCCCC
>Mature Secondary Structure
MNGAQWVIQALRAQGVDMVFGYPGGAIMPVYDALFDGGVEHLLCRHEQGAAIAAIGYARA
CCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHCCCCCCEEEEEEEEEC
TGKVGVCIATSGPGATNLITGLADALLDSVPVVAITGQVGSAFIGTDAFQEIDVLGLSLA
CCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEECCCHHHHHHHHHEEEEE
CTKHSFLVESLDALPGIITEAFAIATSGRTGPVLIDIPKDIQLADGDLTPHLLSLDDQAS
HHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEECCCCCEECCCCCCCEEEECCCCHH
SIQGDLDGARRLMRQATRPILYVGGGVGMAGAVPALRQLITQTGLPAVATLKGLGAPDVD
HHCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCC
DACYLGMLGMHGNQAANLAVQACDLLIAVGARFDDRVTGRLNAFAPHAKVIHLDIDPAEL
CHHHHHEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCHHHH
GKLRQAHIALLGDLNHLLPALAQPLSIDAWRVETQALKAEYRWRYDNPGAGIYAPALLRA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHEEEECCCCCCHHHHHHHHH
ISDRAPDEAVVTTDVGQHQMWAAQHMRFSRPENFITSSGLGTMGFGIPAAIGAQIARLED
HHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHEECC
TVICISGDGSFMMNVQELTTIKRKRLPLKIVLLDNQRLGMVRQWQQLFFDKRYSETTLTD
EEEEEECCCCEEEEHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCC
NPDFLTLASAFNIPGLHITRKDQIPQALDSLFNQPGPFLLHVSIDELENVWPLVPPGAGN
CCCEEEEEHHCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCCCCCCCCCC
ETMLEKSL
CCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3550695; 1379743; 9278503; 1995430; 6154938; 7015336; 3897211 [H]