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The map label for this gene is dapF

Identifier: 85060324

GI number: 85060324

Start: 4024214

End: 4025038

Strand: Reverse

Name: dapF

Synonym: SG2346

Alternate gene names: 85060324

Gene position: 4025038-4024214 (Counterclockwise)

Preceding gene: 85060325

Following gene: 85060323

Centisome position: 96.5

GC content: 57.45

Gene sequence:

>825_bases
ATGCAGTTCTCCAAAATGCACGGCCTGGGTAACGACTTTATGGTCGTGGATGCCGTGACGCAAAATGTTTATTTCTCACC
CGAAATGATCCGCCGCCTGTCGGACCGGCATTGCGGTGTAGGGTTTGATCAATTGCTGGTGGTGGAACCACCCTATGATC
CGGAACTGGATTTCCATTATCGTATTTTTAACGCCGACGGTAGCGAAGTGGCGCAATGCGGCAACGGCGCCCGCTGTTTT
GCCCGTTTTGTGCGCATGAAGAATCTGACCAACAAACGCGATATCCATGTCAGTACCCAAACGGGACGTATGGTATTGAC
CGTCACTGAAGACGATTCGGTACGCGTTAATATGGGCGAGCCGAATTTCGACCCGCAGCAGGTGCTGTTTCGCGCTGCTA
GGGTTGAAAAAACCTATATTATGCGTGCGGCGGAGCAGACGGTGCTCTGCGGCGTGGTGTCCATGGGCAACCCGCACTGC
GTAATCGCCGTGGACAGCGTAGACAACGCGCCGGTCGCGACGCTTGGACCGGTATTGGAGAGCCACGAGCGTTTTCCGGA
ACGGGCCAATATTGGTTTTATGCAGGTGTTGAACCGCGGGCATATCCGCCTGCGGGTCTACGAACGCGGCGCGGGAGAAA
CCCAAGCCTGCGGCAGCGGCGCCTGTGCCGCGGTCGCGGTGGGGATCTCGCAGGGTCAGCTGGCGGAACAGGTACAGGTG
GAACTCACCGGCGGCCGACTCGCCATCAGCTGGCGCGGACCGGGTCATCCGCTGTATATGACTGGCCCGGCCACCCATAT
CTATGACGGATTTATTCATCTATGA

Upstream 100 bases:

>100_bases
CAGCACCGCCGTCGGGACCGAACAGCCGTAATCGCGACGCAGTCGCTTCCTGCCGGCAGACGCGCTATGCTGCGTTATCG
GCTACTCAGCGGAGTGGGAT

Downstream 100 bases:

>100_bases
AGCAAGTCGGCGAACAGGCGGACAGCGCGCAAACGCTGGATGACGAACAGGTGGTGCAGTATCTGCTCACGCACCCGGAT
TTCTTTATCCGTAATGCCCG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MQFSKMHGLGNDFMVVDAVTQNVYFSPEMIRRLSDRHCGVGFDQLLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCF
ARFVRMKNLTNKRDIHVSTQTGRMVLTVTEDDSVRVNMGEPNFDPQQVLFRAARVEKTYIMRAAEQTVLCGVVSMGNPHC
VIAVDSVDNAPVATLGPVLESHERFPERANIGFMQVLNRGHIRLRVYERGAGETQACGSGACAAVAVGISQGQLAEQVQV
ELTGGRLAISWRGPGHPLYMTGPATHIYDGFIHL

Sequences:

>Translated_274_residues
MQFSKMHGLGNDFMVVDAVTQNVYFSPEMIRRLSDRHCGVGFDQLLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCF
ARFVRMKNLTNKRDIHVSTQTGRMVLTVTEDDSVRVNMGEPNFDPQQVLFRAARVEKTYIMRAAEQTVLCGVVSMGNPHC
VIAVDSVDNAPVATLGPVLESHERFPERANIGFMQVLNRGHIRLRVYERGAGETQACGSGACAAVAVGISQGQLAEQVQV
ELTGGRLAISWRGPGHPLYMTGPATHIYDGFIHL
>Mature_274_residues
MQFSKMHGLGNDFMVVDAVTQNVYFSPEMIRRLSDRHCGVGFDQLLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCF
ARFVRMKNLTNKRDIHVSTQTGRMVLTVTEDDSVRVNMGEPNFDPQQVLFRAARVEKTYIMRAAEQTVLCGVVSMGNPHC
VIAVDSVDNAPVATLGPVLESHERFPERANIGFMQVLNRGHIRLRVYERGAGETQACGSGACAAVAVGISQGQLAEQVQV
ELTGGRLAISWRGPGHPLYMTGPATHIYDGFIHL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=274, Percent_Identity=86.4963503649635, Blast_Score=499, Evalue=1e-143,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_SODGM (Q2NQF4)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_456026.1
- HSSP:   P44859
- ProteinModelPortal:   Q2NQF4
- SMR:   Q2NQF4
- STRING:   Q2NQF4
- GeneID:   3867174
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG2346
- NMPDR:   fig|343509.6.peg.5089
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- PhylomeDB:   Q2NQF4
- ProtClustDB:   PRK00450
- BioCyc:   SGLO343509:SG2346-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30225; Mature: 30225

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 73-73 ACT_SITE 217-217

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFSKMHGLGNDFMVVDAVTQNVYFSPEMIRRLSDRHCGVGFDQLLVVEPPYDPELDFHY
CCCHHHCCCCCCEEEEEEEECCEEECHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEE
RIFNADGSEVAQCGNGARCFARFVRMKNLTNKRDIHVSTQTGRMVLTVTEDDSVRVNMGE
EEECCCCHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEECCCCEEEECCC
PNFDPQQVLFRAARVEKTYIMRAAEQTVLCGVVSMGNPHCVIAVDSVDNAPVATLGPVLE
CCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHH
SHERFPERANIGFMQVLNRGHIRLRVYERGAGETQACGSGACAAVAVGISQGQLAEQVQV
HHHCCCCCCCCCHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCEEEEE
ELTGGRLAISWRGPGHPLYMTGPATHIYDGFIHL
EEECCEEEEEECCCCCCEEEECCCHHCCCCEEEC
>Mature Secondary Structure
MQFSKMHGLGNDFMVVDAVTQNVYFSPEMIRRLSDRHCGVGFDQLLVVEPPYDPELDFHY
CCCHHHCCCCCCEEEEEEEECCEEECHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEE
RIFNADGSEVAQCGNGARCFARFVRMKNLTNKRDIHVSTQTGRMVLTVTEDDSVRVNMGE
EEECCCCHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEECCCCEEEECCC
PNFDPQQVLFRAARVEKTYIMRAAEQTVLCGVVSMGNPHCVIAVDSVDNAPVATLGPVLE
CCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHH
SHERFPERANIGFMQVLNRGHIRLRVYERGAGETQACGSGACAAVAVGISQGQLAEQVQV
HHHCCCCCCCCCHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCEEEEE
ELTGGRLAISWRGPGHPLYMTGPATHIYDGFIHL
EEECCEEEEEECCCCCCEEEECCCHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA