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The map label for this gene is kefB [H]

Identifier: 85060273

GI number: 85060273

Start: 3943977

End: 3945590

Strand: Reverse

Name: kefB [H]

Synonym: SG2295

Alternate gene names: 85060273

Gene position: 3945590-3943977 (Counterclockwise)

Preceding gene: 85060275

Following gene: 85060272

Centisome position: 94.59

GC content: 58.3

Gene sequence:

>1614_bases
GTGCTGGGCTATCTGGCCGCCGGCATCGCCATCGACCCCTGGGGCCTGGGCTTTATTCGCGATGTCGATGAGATACTGCA
TTTCTCGGAGCTGGGGGTGGTGTTCTTGATGTTCATCATCGGCCTGGAGCTGAACCCCGCCAAGCTCTGGCACCTGCGGC
ACTCGATATTCGGCGTCGGCGCCGCGCAGGTAGCCATCACCGCGGTGCTGCTGGGTGCCGCGCTTACCTTTACCGGTATG
CGCTGGCAGGCGGCGCTTATCGGCGGCATTGGCATCGCCATGTCCTCTACCGCCATTGCGCTGCAGTTGATGCGCGACAA
AGGCATGAACCGCAATGAGGGCGGCCAATTGGGGTTTTCGGTGTTACTGTTTCAGGATATGGCGGTGATCCCGGCACTGT
CTCTGATTCCCTTTCTCGCAGGCTGCGCCGGCGGGCACGGCGATTGGATGAAGATCAGCATTAAACTGGCGGCATTCTTT
GGAATGCTGGTCGGCGGGCGCTTCCTACTGCGGTCGCTGTTTCGCTATATCGCCTCGGTGAACGTGCACGAGGTGCTTAC
CGCCGCCAGCCTGCTGTTGGTGCTGGGTGCCGCGTTATTCATGGATTTGCGTGGCCTGTCGATGGCCCTTGGCACCTTTA
TCGCCGGCGTGCTGCTGGCTGAAAGCGAGTACCGCCATGAATTCGAGATCGCTATTGAGCCGTTCAAGGGGCTGCTGCTG
GGATTGTTTTTTATCTCGGTCGGCATGGCGCTGAACCTGGGGATCCTGTATGTGCACGTCCTGACAGTGCTGGCCGGCGT
CGCCATCCTGGTGGCCATCAAGGGAACGGTGTTGTATATCCTGGCGCGCCTGTCGGGGCTGAAACGTTCGGTCCGGCTGC
AATTCGCCGGCGTACTGAGCCAGGGCGGGGAATTTGCCTTCGTTTTGTTCTCGGCGGCCTCGGTGCAGAAAATCCTCACC
GGCGATCAATTATCGTTATTGCTGGTGATCGTCACCTTGTCGATGATCACCACCCCGATATTGATGCAATTGATCGATCG
TATCCTGGTCCGGCGTTATAATGCAGTAGAAGAAAACGATGAAAAACCCTTCGTCAACGACGATGACCCGCAGGTTATCA
TTGTCGGCTTCGGCCGTTTCGGTCAGGTGATAGGTCGCCTGCTGATGGCAAATAAAACCCGTATTACGGTGCTTGAGCGT
GATATCAGCGTGGTCAGCTTGATGCGCGGCTATGGTTATAAAGTGTATTACGGCGATGCCACCGAACTGGAGCTGTTGCG
GGCGGCGGGAGCGGAGAAGGCCAGAACGATCGTCGTTGCGGCCAATACGCCTGAAGATAATATGGCCATCATCCGCCTGT
GCCAGCAGCATTTTCCCCAGTTGCAGATTTTGGCGCGCGGGCGTGTCGAAGCGCATGAGCTACTGCAGGTCGGCGTGTCG
CGATTTTCAAGGGAAACGTTCTCCAGCGCTTTGGAGCTGGGGCGCAAGGTGCTGCAATCGTTAGGTATGCATCCACATCA
GGCCTACCGGGCGCAGCAGCATTTCCGCCGGCTGGATATGCGCATGCTGCGTGAGTTGATGCCCCAGGAGGAGGGGGGCA
CAGTTCGCGTGTAA

Upstream 100 bases:

>100_bases
CGAGAGCGCCTATCAGACCCGCGGCGCGTTAGGGGTGGAGAGACAGATATCCTGCGTCTTTTCGCTCTGACCGCAGCACT
GTGCCGCATGCATCGGCGCA

Downstream 100 bases:

>100_bases
AGGAAGCGCGGCGCGAACTGGAAGACATTTTCCAGCGCGAGATGCAACAGGAGCAGCGGCTGCTCGACGGCTGGGGCGAG
GATGATCAACAGGGAGAACG

Product: glutathione-regulated potassium-efflux system protein KefB

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MLGYLAAGIAIDPWGLGFIRDVDEILHFSELGVVFLMFIIGLELNPAKLWHLRHSIFGVGAAQVAITAVLLGAALTFTGM
RWQAALIGGIGIAMSSTAIALQLMRDKGMNRNEGGQLGFSVLLFQDMAVIPALSLIPFLAGCAGGHGDWMKISIKLAAFF
GMLVGGRFLLRSLFRYIASVNVHEVLTAASLLLVLGAALFMDLRGLSMALGTFIAGVLLAESEYRHEFEIAIEPFKGLLL
GLFFISVGMALNLGILYVHVLTVLAGVAILVAIKGTVLYILARLSGLKRSVRLQFAGVLSQGGEFAFVLFSAASVQKILT
GDQLSLLLVIVTLSMITTPILMQLIDRILVRRYNAVEENDEKPFVNDDDPQVIIVGFGRFGQVIGRLLMANKTRITVLER
DISVVSLMRGYGYKVYYGDATELELLRAAGAEKARTIVVAANTPEDNMAIIRLCQQHFPQLQILARGRVEAHELLQVGVS
RFSRETFSSALELGRKVLQSLGMHPHQAYRAQQHFRRLDMRMLRELMPQEEGGTVRV

Sequences:

>Translated_537_residues
MLGYLAAGIAIDPWGLGFIRDVDEILHFSELGVVFLMFIIGLELNPAKLWHLRHSIFGVGAAQVAITAVLLGAALTFTGM
RWQAALIGGIGIAMSSTAIALQLMRDKGMNRNEGGQLGFSVLLFQDMAVIPALSLIPFLAGCAGGHGDWMKISIKLAAFF
GMLVGGRFLLRSLFRYIASVNVHEVLTAASLLLVLGAALFMDLRGLSMALGTFIAGVLLAESEYRHEFEIAIEPFKGLLL
GLFFISVGMALNLGILYVHVLTVLAGVAILVAIKGTVLYILARLSGLKRSVRLQFAGVLSQGGEFAFVLFSAASVQKILT
GDQLSLLLVIVTLSMITTPILMQLIDRILVRRYNAVEENDEKPFVNDDDPQVIIVGFGRFGQVIGRLLMANKTRITVLER
DISVVSLMRGYGYKVYYGDATELELLRAAGAEKARTIVVAANTPEDNMAIIRLCQQHFPQLQILARGRVEAHELLQVGVS
RFSRETFSSALELGRKVLQSLGMHPHQAYRAQQHFRRLDMRMLRELMPQEEGGTVRV
>Mature_537_residues
MLGYLAAGIAIDPWGLGFIRDVDEILHFSELGVVFLMFIIGLELNPAKLWHLRHSIFGVGAAQVAITAVLLGAALTFTGM
RWQAALIGGIGIAMSSTAIALQLMRDKGMNRNEGGQLGFSVLLFQDMAVIPALSLIPFLAGCAGGHGDWMKISIKLAAFF
GMLVGGRFLLRSLFRYIASVNVHEVLTAASLLLVLGAALFMDLRGLSMALGTFIAGVLLAESEYRHEFEIAIEPFKGLLL
GLFFISVGMALNLGILYVHVLTVLAGVAILVAIKGTVLYILARLSGLKRSVRLQFAGVLSQGGEFAFVLFSAASVQKILT
GDQLSLLLVIVTLSMITTPILMQLIDRILVRRYNAVEENDEKPFVNDDDPQVIIVGFGRFGQVIGRLLMANKTRITVLER
DISVVSLMRGYGYKVYYGDATELELLRAAGAEKARTIVVAANTPEDNMAIIRLCQQHFPQLQILARGRVEAHELLQVGVS
RFSRETFSSALELGRKVLQSLGMHPHQAYRAQQHFRRLDMRMLRELMPQEEGGTVRV

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=380, Percent_Identity=26.3157894736842, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1789749, Length=530, Percent_Identity=74.5283018867924, Blast_Score=772, Evalue=0.0,
Organism=Escherichia coli, GI1786232, Length=541, Percent_Identity=42.8835489833641, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI1786685, Length=525, Percent_Identity=28.7619047619048, Blast_Score=162, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 58781; Mature: 58781

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGYLAAGIAIDPWGLGFIRDVDEILHFSELGVVFLMFIIGLELNPAKLWHLRHSIFGVG
CCCCEECCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
AAQVAITAVLLGAALTFTGMRWQAALIGGIGIAMSSTAIALQLMRDKGMNRNEGGQLGFS
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
VLLFQDMAVIPALSLIPFLAGCAGGHGDWMKISIKLAAFFGMLVGGRFLLRSLFRYIASV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCC
NVHEVLTAASLLLVLGAALFMDLRGLSMALGTFIAGVLLAESEYRHEFEIAIEPFKGLLL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHH
GLFFISVGMALNLGILYVHVLTVLAGVAILVAIKGTVLYILARLSGLKRSVRLQFAGVLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QGGEFAFVLFSAASVQKILTGDQLSLLLVIVTLSMITTPILMQLIDRILVRRYNAVEEND
CCCCEEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EKPFVNDDDPQVIIVGFGRFGQVIGRLLMANKTRITVLERDISVVSLMRGYGYKVYYGDA
CCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCEEEEECCC
TELELLRAAGAEKARTIVVAANTPEDNMAIIRLCQQHFPQLQILARGRVEAHELLQVGVS
HHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEHHCCCCHHHHHHHHHHH
RFSRETFSSALELGRKVLQSLGMHPHQAYRAQQHFRRLDMRMLRELMPQEEGGTVRV
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLGYLAAGIAIDPWGLGFIRDVDEILHFSELGVVFLMFIIGLELNPAKLWHLRHSIFGVG
CCCCEECCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
AAQVAITAVLLGAALTFTGMRWQAALIGGIGIAMSSTAIALQLMRDKGMNRNEGGQLGFS
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
VLLFQDMAVIPALSLIPFLAGCAGGHGDWMKISIKLAAFFGMLVGGRFLLRSLFRYIASV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCC
NVHEVLTAASLLLVLGAALFMDLRGLSMALGTFIAGVLLAESEYRHEFEIAIEPFKGLLL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHH
GLFFISVGMALNLGILYVHVLTVLAGVAILVAIKGTVLYILARLSGLKRSVRLQFAGVLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QGGEFAFVLFSAASVQKILTGDQLSLLLVIVTLSMITTPILMQLIDRILVRRYNAVEEND
CCCCEEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EKPFVNDDDPQVIIVGFGRFGQVIGRLLMANKTRITVLERDISVVSLMRGYGYKVYYGDA
CCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCEEEEECCC
TELELLRAAGAEKARTIVVAANTPEDNMAIIRLCQQHFPQLQILARGRVEAHELLQVGVS
HHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEHHCCCCHHHHHHHHHHH
RFSRETFSSALELGRKVLQSLGMHPHQAYRAQQHFRRLDMRMLRELMPQEEGGTVRV
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA