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The map label for this gene is slyD [H]

Identifier: 85060271

GI number: 85060271

Start: 3943056

End: 3943574

Strand: Reverse

Name: slyD [H]

Synonym: SG2293

Alternate gene names: 85060271

Gene position: 3943574-3943056 (Counterclockwise)

Preceding gene: 85060272

Following gene: 85060269

Centisome position: 94.54

GC content: 54.72

Gene sequence:

>519_bases
ATGAAAGTAGCAAAAGATCGGGTGGTCAGTCTGGCCTACCAGTTGAGAACAGAAGACGGTGTGTTGGTCGATGAATCCCC
GGTGAGCGCACCGTTGGAGTACCTCCATGGCCACGGCTCGCTGATTTCTGAGCTGGAAAAAGCGCTGGATGATCGCGGTG
CGGGAGACATCTTCGATATCAGCATCGAAGCCAATGATGCTTACGGCCCTTACGATGAAAATCTGGTGCAGCGCGTACCC
AAAGATGTATTCATGGGCGTCGACGAACTGGAAGTGGGAATGCGTTTCCTTGCCGAAACGGATCAGGATCAGGTACCGGT
CGAAATTACTGAAATCGATGGCGATCATGTCGTGGTTGACGGTAATCCTATGCTGGCTGGCCAGAACCTCAACTTTCATG
TGGAAGTCATCGCCATTCGCGAAGCCACCGCGGAAGAGCTGGCCCGCGGCCATGTCCATGGTGCGCACGGCCATGATCAC
GACCATGACCACGGTGATGGCTGCTGCTGCGGTCACTAA

Upstream 100 bases:

>100_bases
GGAGTAATGTTATTAGCAGCTTTTTTACGGCTAAAGCGTACATACGTGCGGTTTTTAGTTAGAATCAGCGCCAAATGAGT
TTCCAATGGTGGGAGAGAGC

Downstream 100 bases:

>100_bases
GCATGGGGTTCGGGACCCTGAGCGGGCCCCGGCCTCACCCGCTCAGTAATGGGGCGGGGGCGTTTCCTCGCTTTGCGAGG
CGATGATTGAGGTGTTCTGC

Product: FKBP-type peptidyl-prolyl cis-trans isomerase

Products: NA

Alternate protein names: PPIase; Metallochaperone slyD [H]

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MKVAKDRVVSLAYQLRTEDGVLVDESPVSAPLEYLHGHGSLISELEKALDDRGAGDIFDISIEANDAYGPYDENLVQRVP
KDVFMGVDELEVGMRFLAETDQDQVPVEITEIDGDHVVVDGNPMLAGQNLNFHVEVIAIREATAEELARGHVHGAHGHDH
DHDHGDGCCCGH

Sequences:

>Translated_172_residues
MKVAKDRVVSLAYQLRTEDGVLVDESPVSAPLEYLHGHGSLISELEKALDDRGAGDIFDISIEANDAYGPYDENLVQRVP
KDVFMGVDELEVGMRFLAETDQDQVPVEITEIDGDHVVVDGNPMLAGQNLNFHVEVIAIREATAEELARGHVHGAHGHDH
DHDHGDGCCCGH
>Mature_172_residues
MKVAKDRVVSLAYQLRTEDGVLVDESPVSAPLEYLHGHGSLISELEKALDDRGAGDIFDISIEANDAYGPYDENLVQRVP
KDVFMGVDELEVGMRFLAETDQDQVPVEITEIDGDHVVVDGNPMLAGQNLNFHVEVIAIREATAEELARGHVHGAHGHDH
DHDHGDGCCCGH

Specific function: Also involved in hydrogenase metallocenter assembly, probably by participating in the nickel insertion step. This function in hydrogenase biosynthesis requires chaperone activity and the presence of the metal-binding domain, but not PPIase activity [H]

COG id: COG1047

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerases 2

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1789748, Length=148, Percent_Identity=81.0810810810811, Blast_Score=235, Evalue=1e-63,
Organism=Escherichia coli, GI1786211, Length=144, Percent_Identity=32.6388888888889, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179 [H]

Pfam domain/function: PF00254 FKBP_C [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 18772; Mature: 18772

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAKDRVVSLAYQLRTEDGVLVDESPVSAPLEYLHGHGSLISELEKALDDRGAGDIFDI
CCCCHHHHHHHHHEEECCCCEEEECCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEE
SIEANDAYGPYDENLVQRVPKDVFMGVDELEVGMRFLAETDQDQVPVEITEIDGDHVVVD
EEECCCCCCCCCHHHHHHCCHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEC
GNPMLAGQNLNFHVEVIAIREATAEELARGHVHGAHGHDHDHDHGDGCCCGH
CCEEEECCCCCEEEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCC
>Mature Secondary Structure
MKVAKDRVVSLAYQLRTEDGVLVDESPVSAPLEYLHGHGSLISELEKALDDRGAGDIFDI
CCCCHHHHHHHHHEEECCCCEEEECCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEE
SIEANDAYGPYDENLVQRVPKDVFMGVDELEVGMRFLAETDQDQVPVEITEIDGDHVVVD
EEECCCCCCCCCHHHHHHCCHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEC
GNPMLAGQNLNFHVEVIAIREATAEELARGHVHGAHGHDHDHDHGDGCCCGH
CCEEEECCCCCEEEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]