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The map label for this gene is intB [H]

Identifier: 85060082

GI number: 85060082

Start: 3642187

End: 3643062

Strand: Reverse

Name: intB [H]

Synonym: SG2104

Alternate gene names: 85060082

Gene position: 3643062-3642187 (Counterclockwise)

Preceding gene: 85060085

Following gene: 85060079

Centisome position: 87.34

GC content: 49.32

Gene sequence:

>876_bases
ATGGCTCTGAGTGATGTGAAGGTTCGTGCTGCCAAGCCTGAAGCAAAAGCCTATAAGCTTAAAGACGGCGAAGGCATGGT
ATTGCTAGTTCACCCTAACGGTTCAAAATACTGGCGGTTACGTTATCGCTTCGGCGGCAAAGAAAAGATGCTGGAGTTGG
GACAATACCCTGAAGTTTTGTTAGCGGACGCCCGATCACGTAGAGACGAAGCGCGTAAGCTGTTAGCTAACGGCATTAAT
CCTATCGAGAATAAGAAAGCCATTAAGGTAGAACAAGAGCAGGAAGCGATAACTTTTGAAGTGGTCGCCAGAGACTGGCA
CGCCAGCAATCAGAAATGGTCTGCATCGCATAGTGCTCGGGTATTGAAAAGCTTGGAAGATAATCTTTTTACTGCCATTG
GTAAGCGGAATATTGCGGAACTGAAGACGCGAGATCTGCTTGTACCCATCAAGGCGGTGGAATCATCCGGACGACTCGAA
GTCGCCGCCCGCTTACAGCAGCGCACTACCGCAATTATGTGCTTTGCTGTGCAGAGTGGCTTAATCGATTACAACCCTGC
ACAAGAGATTGCTGGTGCGGTTGCTACTGCGAAAAGACAGCATCGTGCGGCTCTGGAGCTTAACCGCATACCCGAATTAC
TTCATCGCATTGATCACTATTCTGGAAGACCATTAACCCGATTGGCTGTTGAACTCACTTTGTTGGTTTTCATCCGTTCA
AGCGAACTGCGTTTTGCTCGCTGGCCTGAAGTGGATTTTGACACGGCAAAGTGGACGATTCCTGGAGAGCGTGAACCATT
GGAAGGCGTTAAGCACTCTCAAGTGGTTCGAAGATGCGGACGCTTCATCTTGTACCGTTATCGCGGCAGGCTCTAG

Upstream 100 bases:

>100_bases
GATTCAATCTACATCAACTTACTGTTGCGGGTACAACTACGGGTATATCCCGATTCGATAATGTTTGTACCCACACAAAA
CCTTTGAAAGAATACTCATC

Downstream 100 bases:

>100_bases
CCATTTTGGAAAAGATCAAAAGCATGAACGGGAATCGAGAGCTGATTTTTGTGGGCGATCACGATCCGCGAAAGCCCATG
AGTGAGAACACTGTCAATAA

Product: putative phage integrase

Products: NA

Alternate protein names: Int(P4) [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MALSDVKVRAAKPEAKAYKLKDGEGMVLLVHPNGSKYWRLRYRFGGKEKMLELGQYPEVLLADARSRRDEARKLLANGIN
PIENKKAIKVEQEQEAITFEVVARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
VAARLQQRTTAIMCFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRIDHYSGRPLTRLAVELTLLVFIRS
SELRFARWPEVDFDTAKWTIPGEREPLEGVKHSQVVRRCGRFILYRYRGRL

Sequences:

>Translated_291_residues
MALSDVKVRAAKPEAKAYKLKDGEGMVLLVHPNGSKYWRLRYRFGGKEKMLELGQYPEVLLADARSRRDEARKLLANGIN
PIENKKAIKVEQEQEAITFEVVARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
VAARLQQRTTAIMCFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRIDHYSGRPLTRLAVELTLLVFIRS
SELRFARWPEVDFDTAKWTIPGEREPLEGVKHSQVVRRCGRFILYRYRGRL
>Mature_290_residues
ALSDVKVRAAKPEAKAYKLKDGEGMVLLVHPNGSKYWRLRYRFGGKEKMLELGQYPEVLLADARSRRDEARKLLANGINP
IENKKAIKVEQEQEAITFEVVARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLEV
AARLQQRTTAIMCFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRIDHYSGRPLTRLAVELTLLVFIRSS
ELRFARWPEVDFDTAKWTIPGEREPLEGVKHSQVVRRCGRFILYRYRGRL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=266, Percent_Identity=35.3383458646617, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI145693166, Length=265, Percent_Identity=35.4716981132075, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1788690, Length=255, Percent_Identity=31.3725490196078, Blast_Score=143, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 33189; Mature: 33058

Theoretical pI: Translated: 10.52; Mature: 10.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALSDVKVRAAKPEAKAYKLKDGEGMVLLVHPNGSKYWRLRYRFGGKEKMLELGQYPEVL
CCCCCCEEEECCCCCCEEEEECCCCEEEEEECCCCCEEEEEEEECCHHHHHHHCCCCHHH
LADARSRRDEARKLLANGINPIENKKAIKVEQEQEAITFEVVARDWHASNQKWSASHSAR
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHEEEEEEEHHHCCCCCCCCCHHHHH
VLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLEVAARLQQRTTAIMCFAVQSG
HHHHHHHHHHHHHCCCCHHHHHHHHEEEEHHHHCCCCCHHHHHHHHHHHHHHEEEEHHCC
LIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRIDHYSGRPLTRLAVELTLLVFIRS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEC
SELRFARWPEVDFDTAKWTIPGEREPLEGVKHSQVVRRCGRFILYRYRGRL
CCCCEEECCCCCCCCCEEECCCCCCCHHCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ALSDVKVRAAKPEAKAYKLKDGEGMVLLVHPNGSKYWRLRYRFGGKEKMLELGQYPEVL
CCCCCEEEECCCCCCEEEEECCCCEEEEEECCCCCEEEEEEEECCHHHHHHHCCCCHHH
LADARSRRDEARKLLANGINPIENKKAIKVEQEQEAITFEVVARDWHASNQKWSASHSAR
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHEEEEEEEHHHCCCCCCCCCHHHHH
VLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLEVAARLQQRTTAIMCFAVQSG
HHHHHHHHHHHHHCCCCHHHHHHHHEEEEHHHHCCCCCHHHHHHHHHHHHHHEEEEHHCC
LIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRIDHYSGRPLTRLAVELTLLVFIRS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEC
SELRFARWPEVDFDTAKWTIPGEREPLEGVKHSQVVRRCGRFILYRYRGRL
CCCCEEECCCCCCCCCEEECCCCCCCHHCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]