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The map label for this gene is thyA

Identifier: 85059955

GI number: 85059955

Start: 3413494

End: 3414288

Strand: Reverse

Name: thyA

Synonym: SG1977

Alternate gene names: 85059955

Gene position: 3414288-3413494 (Counterclockwise)

Preceding gene: 85059956

Following gene: 85059954

Centisome position: 81.85

GC content: 56.73

Gene sequence:

>795_bases
ATGAAACAGTATTTGGATTTGATGCGTAGGGTGCGTGCGCAGGGCACGCCGAAAGCGGACCGTACCGGTACCGGTACCCT
ATCAATATTCGGCCATCAGATGCGCTTTGATTTGCGTCAGGGATTCCTGCTGGTGACCACCAAACGCTGCCATTTGCGGT
CGATCATCCATGAGTTGCTGTGGTTCCTTAACGGCGATACCAATATCGCTTATTTGCAGCAGAATAACGTTTCCATTTGG
GATGAATGGGCGGATGACAACGGCGATTTGGGGCCGGTGTATGGGCGCCAGTGGCGCGCCTGGGGCACCAGCGACGGCCG
CCAGATAGATCAACTGGCGGAAGTGGTGCGCCAGCTCAAGCAGGATCCGGATTCCCGGCGCATCATCGTTTCCGCCTGGA
ACGTCGGCGAACTGGACAAAATGGCCCTGGCGCCGTGCCATGCGTTGTTTCAGTTTTATGTTGCCAACGGCACCTTGTCA
TGTCAGCTCTATCAGCGTTCGTGCGATATTTTCCTCGGCCTGCCGTTCAATATCGCCAGCTATGCCTTGCTGGTGCATAT
GCTGGCGCAGCAGTGCGATTTGCAGGTCGGTGATTTCGTCTGGACCGGCGGCGATACCCACCTCTACAGTAACCATTTGC
AGCAGGCGGACCTGCAGCTAACCCGCGATCCGCTGCCGCTGCCGCAGCTGCTGATTAAGCGCCGTCCGGCGACATTGTTC
GATTACCGTTTCGAGGATTTTGAGCTGACGGGTTACGATCCCCATCCGGCAATCAAGGCGCCGGTGGCTGTTTAA

Upstream 100 bases:

>100_bases
CATGGGGCAGATTCTATCGCTGCCGATGATTCTGGCCGGTGTGATTATGATGGCCTGGGCCTACCGCCGTCACCCCCAGC
AACACCTGTCTTGAGGTATT

Downstream 100 bases:

>100_bases
GCTGCCGCCGCCGGGCCGCACGGGCGGCGAAATGTCTACGCGGCCCGGCGCCTGCCGGGTGCTATCGTTTGTATCGTGCT
CGGTTCGCGCCTAATCCTGA

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKQYLDLMRRVRAQGTPKADRTGTGTLSIFGHQMRFDLRQGFLLVTTKRCHLRSIIHELLWFLNGDTNIAYLQQNNVSIW
DEWADDNGDLGPVYGRQWRAWGTSDGRQIDQLAEVVRQLKQDPDSRRIIVSAWNVGELDKMALAPCHALFQFYVANGTLS
CQLYQRSCDIFLGLPFNIASYALLVHMLAQQCDLQVGDFVWTGGDTHLYSNHLQQADLQLTRDPLPLPQLLIKRRPATLF
DYRFEDFELTGYDPHPAIKAPVAV

Sequences:

>Translated_264_residues
MKQYLDLMRRVRAQGTPKADRTGTGTLSIFGHQMRFDLRQGFLLVTTKRCHLRSIIHELLWFLNGDTNIAYLQQNNVSIW
DEWADDNGDLGPVYGRQWRAWGTSDGRQIDQLAEVVRQLKQDPDSRRIIVSAWNVGELDKMALAPCHALFQFYVANGTLS
CQLYQRSCDIFLGLPFNIASYALLVHMLAQQCDLQVGDFVWTGGDTHLYSNHLQQADLQLTRDPLPLPQLLIKRRPATLF
DYRFEDFELTGYDPHPAIKAPVAV
>Mature_264_residues
MKQYLDLMRRVRAQGTPKADRTGTGTLSIFGHQMRFDLRQGFLLVTTKRCHLRSIIHELLWFLNGDTNIAYLQQNNVSIW
DEWADDNGDLGPVYGRQWRAWGTSDGRQIDQLAEVVRQLKQDPDSRRIIVSAWNVGELDKMALAPCHALFQFYVANGTLS
CQLYQRSCDIFLGLPFNIASYALLVHMLAQQCDLQVGDFVWTGGDTHLYSNHLQQADLQLTRDPLPLPQLLIKRRPATLF
DYRFEDFELTGYDPHPAIKAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=282, Percent_Identity=48.5815602836879, Blast_Score=269, Evalue=2e-72,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=81.0606060606061, Blast_Score=468, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI71993377, Length=283, Percent_Identity=46.643109540636, Blast_Score=261, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6324648, Length=292, Percent_Identity=39.3835616438356, Blast_Score=197, Evalue=1e-51,
Organism=Drosophila melanogaster, GI17137556, Length=284, Percent_Identity=52.112676056338, Blast_Score=285, Evalue=3e-77,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_SODGM (Q2NRH3)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_455657.1
- HSSP:   P0A884
- ProteinModelPortal:   Q2NRH3
- SMR:   Q2NRH3
- STRING:   Q2NRH3
- GeneID:   3866785
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG1977
- NMPDR:   fig|343509.6.peg.4270
- eggNOG:   COG0207
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- PhylomeDB:   Q2NRH3
- ProtClustDB:   PRK01827
- BioCyc:   SGLO343509:SG1977-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 30250; Mature: 30250

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 146-146

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLDLMRRVRAQGTPKADRTGTGTLSIFGHQMRFDLRQGFLLVTTKRCHLRSIIHELL
CHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHCCEEEEEECHHHHHHHHHHHH
WFLNGDTNIAYLQQNNVSIWDEWADDNGDLGPVYGRQWRAWGTSDGRQIDQLAEVVRQLK
HHCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHH
QDPDSRRIIVSAWNVGELDKMALAPCHALFQFYVANGTLSCQLYQRSCDIFLGLPFNIAS
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEECCHHHHH
YALLVHMLAQQCDLQVGDFVWTGGDTHLYSNHLQQADLQLTRDPLPLPQLLIKRRPATLF
HHHHHHHHHHHCCCEECCEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEE
DYRFEDFELTGYDPHPAIKAPVAV
EEECCCEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MKQYLDLMRRVRAQGTPKADRTGTGTLSIFGHQMRFDLRQGFLLVTTKRCHLRSIIHELL
CHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHCCEEEEEECHHHHHHHHHHHH
WFLNGDTNIAYLQQNNVSIWDEWADDNGDLGPVYGRQWRAWGTSDGRQIDQLAEVVRQLK
HHCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHH
QDPDSRRIIVSAWNVGELDKMALAPCHALFQFYVANGTLSCQLYQRSCDIFLGLPFNIAS
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEECCHHHHH
YALLVHMLAQQCDLQVGDFVWTGGDTHLYSNHLQQADLQLTRDPLPLPQLLIKRRPATLF
HHHHHHHHHHHCCCEECCEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEE
DYRFEDFELTGYDPHPAIKAPVAV
EEECCCEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA