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The map label for this gene is tilS [H]

Identifier: 85059904

GI number: 85059904

Start: 3324664

End: 3326292

Strand: Reverse

Name: tilS [H]

Synonym: SG1926

Alternate gene names: 85059904

Gene position: 3326292-3324664 (Counterclockwise)

Preceding gene: 85059905

Following gene: 85059902

Centisome position: 79.75

GC content: 64.58

Gene sequence:

>1629_bases
ATGAAGCCTGATGCCTATGAGCGAGTGCAGGTGCTGCGCCGTCACGTCGCCGCCCGTGTCGCGCCTTACCGCGCCCTGCT
GCTGGCGTTCAGCGGCGGTCTGGATTCCACGGTATTGCTGGATGCCCTCGCCGCGTTACGCGATGGCGACGGCCTCTCCG
GCGGTGACGGCGCGCAGACGCTGCGGGCGGTTCATGTCCATCACGCCTTGAGCCGCCACGCAGATGAGTGGGCCGCGCAC
TGCGCGCAGGAATGTCGTCGGCGCGACGTCGCCTTTACTGTGGTACATGTCGTCGTCGACGCGACGTCGGAGGGTATCGA
GGCGGCGGCGCGCGGCGCGCGTTATGGGGCTCTGGCGGCGGCGCTGACACAGGATGAGGTGCTGATTACCGCCCAGCATC
AGGACGATCAGGCGGAAACGCTGCTGTTGGCTTTGAAACGCGGCAGCGGGCCGGCCGGTTTGGCGTCAATGGCGGTGGAT
GCGCCGTATTTAGGCCACCGCCTGGTGCGGCCATTGCTGGACTTCAGTCGGGGGGAGCTAGAAGCGTATGCCCAGGCGCG
CGGGCTTCACTGGACTGAGGACGACAGCAATACCGATCTGCGGTTCGATCGCAATTTTTTGCGCCTACGCATCCTGCCAC
CTTTGCGGCAGCGCTGGCCGCAATTTGCCAACGCCGTAGCGCGTAGCGCGCAGCTTTGCGCCGAGCAGGAACAGCTTCTC
GATGAACTGCTGGCGGAAACATTGGCAGATGTTATCGCACCAGACGAGTCGTTACGCTTGACGGCGCTAGCGACGATGAG
CGAAAGCAAGCGCGCGGCCTTGTTACGCCGCTGGCTGGCCAGCCTCGGCGTAAGAATGCCTGCACGGGCGCAACTGGCGC
GCCTGTGGCAGGAAGTGGCACTCAGCCGGCGCGACGCCGTTGCACAACTGAAACTCGACGGCCGGCTCGTAAGGCGCTTT
CGCGATCGTCTTTATGTCGTGCCGCGTTTGCCGCCGCTTACGGACGGCGAGGCTGTCTTCCCCTGGCCGAGGGAAAGTGA
ACGTCTTGCGTTGCCCGCCGGTCTGGGGAACCTCTATCGACGCCCGGTTGACGCCGGTAGCGCGGTACCGGGGAAACCAT
TCATCGCGGCGGTGACGGGAGGCGGCGGTTTAGAGGAACGGCAGGACGGCAGTGCAAGTTGCGCCTCAGACGCTGACCAT
GCCGCTAATACCGACGCCAATGCCGACGCTAGCGCTAACTATTCCCCCAGCGCCAGCTATGCCGGCAGCACAGCGGCGCC
GCTGGCCGTTGTGCGCGCACCTTTGTCCGGCGAACAGGTGTCGGTGCGTTTCGGCCCCGTGCATGGCCTGTTGTATATTG
TGGGGCGGCGCCGCGGCCGAACGTTGAAAAAAATCTGGCAAGAGCTGGATATCCCGCCGTGGCGGCGCAGCAGCACGCCG
TTGCTGTTTTATAATGACCAACTCATTACGGCGCTGGGCGTCTTCGTGACCTGGGAAGGCGCGGTGCGTGAGCATCACGC
GCAATGGCAGCTTTTTTGGCGGCCGGACAGTAATGGCGCGATGGCCGCCGCGCCAGAGGGCGGCGGTCAGAACGGCGTGT
TATCAGACGAGGAGAGCGGGAGTCATTAG

Upstream 100 bases:

>100_bases
GAGTTGTTCTCCTTTCCCGGCGCGCCGGCACGGGTCGATCCGCTGACCGGCAAAAAATTTACTTTCTTTACCGATCCCTA
TCAATTACCGCTGGAGCTGT

Downstream 100 bases:

>100_bases
CGGGCTGCTGGCGCAGCCCGCGTGAGGGAGAGGGAATTATTCTGCGCTGACCACCACGGTACCCAGTTCGGGATGGGTGA
AACTGGCAATATGATCCAGC

Product: cell cycle protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MKPDAYERVQVLRRHVAARVAPYRALLLAFSGGLDSTVLLDALAALRDGDGLSGGDGAQTLRAVHVHHALSRHADEWAAH
CAQECRRRDVAFTVVHVVVDATSEGIEAAARGARYGALAAALTQDEVLITAQHQDDQAETLLLALKRGSGPAGLASMAVD
APYLGHRLVRPLLDFSRGELEAYAQARGLHWTEDDSNTDLRFDRNFLRLRILPPLRQRWPQFANAVARSAQLCAEQEQLL
DELLAETLADVIAPDESLRLTALATMSESKRAALLRRWLASLGVRMPARAQLARLWQEVALSRRDAVAQLKLDGRLVRRF
RDRLYVVPRLPPLTDGEAVFPWPRESERLALPAGLGNLYRRPVDAGSAVPGKPFIAAVTGGGGLEERQDGSASCASDADH
AANTDANADASANYSPSASYAGSTAAPLAVVRAPLSGEQVSVRFGPVHGLLYIVGRRRGRTLKKIWQELDIPPWRRSSTP
LLFYNDQLITALGVFVTWEGAVREHHAQWQLFWRPDSNGAMAAAPEGGGQNGVLSDEESGSH

Sequences:

>Translated_542_residues
MKPDAYERVQVLRRHVAARVAPYRALLLAFSGGLDSTVLLDALAALRDGDGLSGGDGAQTLRAVHVHHALSRHADEWAAH
CAQECRRRDVAFTVVHVVVDATSEGIEAAARGARYGALAAALTQDEVLITAQHQDDQAETLLLALKRGSGPAGLASMAVD
APYLGHRLVRPLLDFSRGELEAYAQARGLHWTEDDSNTDLRFDRNFLRLRILPPLRQRWPQFANAVARSAQLCAEQEQLL
DELLAETLADVIAPDESLRLTALATMSESKRAALLRRWLASLGVRMPARAQLARLWQEVALSRRDAVAQLKLDGRLVRRF
RDRLYVVPRLPPLTDGEAVFPWPRESERLALPAGLGNLYRRPVDAGSAVPGKPFIAAVTGGGGLEERQDGSASCASDADH
AANTDANADASANYSPSASYAGSTAAPLAVVRAPLSGEQVSVRFGPVHGLLYIVGRRRGRTLKKIWQELDIPPWRRSSTP
LLFYNDQLITALGVFVTWEGAVREHHAQWQLFWRPDSNGAMAAAPEGGGQNGVLSDEESGSH
>Mature_542_residues
MKPDAYERVQVLRRHVAARVAPYRALLLAFSGGLDSTVLLDALAALRDGDGLSGGDGAQTLRAVHVHHALSRHADEWAAH
CAQECRRRDVAFTVVHVVVDATSEGIEAAARGARYGALAAALTQDEVLITAQHQDDQAETLLLALKRGSGPAGLASMAVD
APYLGHRLVRPLLDFSRGELEAYAQARGLHWTEDDSNTDLRFDRNFLRLRILPPLRQRWPQFANAVARSAQLCAEQEQLL
DELLAETLADVIAPDESLRLTALATMSESKRAALLRRWLASLGVRMPARAQLARLWQEVALSRRDAVAQLKLDGRLVRRF
RDRLYVVPRLPPLTDGEAVFPWPRESERLALPAGLGNLYRRPVDAGSAVPGKPFIAAVTGGGGLEERQDGSASCASDADH
AANTDANADASANYSPSASYAGSTAAPLAVVRAPLSGEQVSVRFGPVHGLLYIVGRRRGRTLKKIWQELDIPPWRRSSTP
LLFYNDQLITALGVFVTWEGAVREHHAQWQLFWRPDSNGAMAAAPEGGGQNGVLSDEESGSH

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=376, Percent_Identity=48.1382978723404, Blast_Score=293, Evalue=2e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 58937; Mature: 58937

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPDAYERVQVLRRHVAARVAPYRALLLAFSGGLDSTVLLDALAALRDGDGLSGGDGAQT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHH
LRAVHVHHALSRHADEWAAHCAQECRRRDVAFTVVHVVVDATSEGIEAAARGARYGALAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEHHHHHHCCHHHHHHHHCCCHHHHHHH
ALTQDEVLITAQHQDDQAETLLLALKRGSGPAGLASMAVDAPYLGHRLVRPLLDFSRGEL
HHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCHH
EAYAQARGLHWTEDDSNTDLRFDRNFLRLRILPPLRQRWPQFANAVARSAQLCAEQEQLL
HHHHHHCCCCCCCCCCCCCEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DELLAETLADVIAPDESLRLTALATMSESKRAALLRRWLASLGVRMPARAQLARLWQEVA
HHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LSRRDAVAQLKLDGRLVRRFRDRLYVVPRLPPLTDGEAVFPWPRESERLALPAGLGNLYR
HHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCEECCCCCCCCCEECCCCHHHHHH
RPVDAGSAVPGKPFIAAVTGGGGLEERQDGSASCASDADHAANTDANADASANYSPSASY
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AGSTAAPLAVVRAPLSGEQVSVRFGPVHGLLYIVGRRRGRTLKKIWQELDIPPWRRSSTP
CCCCCCCHHHHCCCCCCCEEEEEECCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCC
LLFYNDQLITALGVFVTWEGAVREHHAQWQLFWRPDSNGAMAAAPEGGGQNGVLSDEESG
EEEECCHHHHHHHHHHEECHHHHHCCCCEEEEEEECCCCCEEECCCCCCCCCCCCCCCCC
SH
CC
>Mature Secondary Structure
MKPDAYERVQVLRRHVAARVAPYRALLLAFSGGLDSTVLLDALAALRDGDGLSGGDGAQT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHH
LRAVHVHHALSRHADEWAAHCAQECRRRDVAFTVVHVVVDATSEGIEAAARGARYGALAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEHHHHHHCCHHHHHHHHCCCHHHHHHH
ALTQDEVLITAQHQDDQAETLLLALKRGSGPAGLASMAVDAPYLGHRLVRPLLDFSRGEL
HHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCHH
EAYAQARGLHWTEDDSNTDLRFDRNFLRLRILPPLRQRWPQFANAVARSAQLCAEQEQLL
HHHHHHCCCCCCCCCCCCCEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DELLAETLADVIAPDESLRLTALATMSESKRAALLRRWLASLGVRMPARAQLARLWQEVA
HHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LSRRDAVAQLKLDGRLVRRFRDRLYVVPRLPPLTDGEAVFPWPRESERLALPAGLGNLYR
HHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCEECCCCCCCCCEECCCCHHHHHH
RPVDAGSAVPGKPFIAAVTGGGGLEERQDGSASCASDADHAANTDANADASANYSPSASY
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AGSTAAPLAVVRAPLSGEQVSVRFGPVHGLLYIVGRRRGRTLKKIWQELDIPPWRRSSTP
CCCCCCCHHHHCCCCCCCEEEEEECCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCC
LLFYNDQLITALGVFVTWEGAVREHHAQWQLFWRPDSNGAMAAAPEGGGQNGVLSDEESG
EEEECCHHHHHHHHHHEECHHHHHCCCCEEEEEEECCCCCEEECCCCCCCCCCCCCCCCC
SH
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA