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The map label for this gene is yfhA [H]

Identifier: 85059755

GI number: 85059755

Start: 3033599

End: 3034378

Strand: Reverse

Name: yfhA [H]

Synonym: SG1777

Alternate gene names: 85059755

Gene position: 3034378-3033599 (Counterclockwise)

Preceding gene: 85059756

Following gene: 85059754

Centisome position: 72.75

GC content: 59.36

Gene sequence:

>780_bases
ATGACGGCCCGCAGACATGCCCATCTCTTGCTGGTGGATGATGATACCAGCCTGTTGAAACTGCTTGGCATGCGGCTGAC
TAGTGAAGGGTTCACGGTGACTACTGCGGAGAACGGTCAGGAGGCGCTACGGCTGTTGCAGCGCGGTAAAATCGACCTGG
TCATCAGCGACTTGCGTATGGATGAGATGGACGGTATCGCGCTGTTCAAGGCAATACAGCAGCAGCCTGGTTTACCGGTG
ATCATTCTTACCGCCCATGGCTCGATACCGGACGCGGTGTCCGCCACCCAGGAGGGGGTGTTCAGCTTTCTGACCAAACC
GGTGGATCGCGACGCGCTGTATTGCGCCATTGATGGGGCGCTGGCGTTGACCCGTCTCGCCGGCGACGAACGCTGGTGGG
ATGCCATCGTCACCCGTAGCCCGCTCATGCTGCGTTTGCTGGAGCAGGCGAAGATGGTGGCCCAATCGGATGTCAGTATC
CTGATAAGCGGTCAGAGCGGCACCGGCAAAGAGATTCTGGCGAAGGTAATCCACGCCGCCAGCCCGCGCGCCCACGCCCG
GGGGGCTTTCACCGGCGTGGTCAGCCAGCGTGAAGGGTTGTTTCAGACCGCCGATGGGGGCACGCTTTTTCTCGATGAGA
TAGGCGATATGCCGCTGTCATTACAGGTTAAACTGCTACGGGTGTTGCAGGAGCGCAAGGTGCGTCCACTGGGCAGCGAC
CGCGATTGCGCTATTGATGTACGTATTTTGTCTCCACCGATTGGGATTTGCCCAAGGTGA

Upstream 100 bases:

>100_bases
GGGAGGAGGGCTCGCGCGCTGTTGACGGCGATTCGGCGGATGCGTCCCTCGCCCATGTCCTTCCTCCCGCTGCAGTGCCC
AAGACGCGAGAAACACGCCC

Downstream 100 bases:

>100_bases
TGGCGGCGGGGGAATTTCGTGAGGATCTGTATTATCGGTTAAATGTCGCCGCTTGAAACTGCCCGCCTTGCATGAGCGGG
CGGAGGATATTCCCTTGCTG

Product: putative two-component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MTARRHAHLLLVDDDTSLLKLLGMRLTSEGFTVTTAENGQEALRLLQRGKIDLVISDLRMDEMDGIALFKAIQQQPGLPV
IILTAHGSIPDAVSATQEGVFSFLTKPVDRDALYCAIDGALALTRLAGDERWWDAIVTRSPLMLRLLEQAKMVAQSDVSI
LISGQSGTGKEILAKVIHAASPRAHARGAFTGVVSQREGLFQTADGGTLFLDEIGDMPLSLQVKLLRVLQERKVRPLGSD
RDCAIDVRILSPPIGICPR

Sequences:

>Translated_259_residues
MTARRHAHLLLVDDDTSLLKLLGMRLTSEGFTVTTAENGQEALRLLQRGKIDLVISDLRMDEMDGIALFKAIQQQPGLPV
IILTAHGSIPDAVSATQEGVFSFLTKPVDRDALYCAIDGALALTRLAGDERWWDAIVTRSPLMLRLLEQAKMVAQSDVSI
LISGQSGTGKEILAKVIHAASPRAHARGAFTGVVSQREGLFQTADGGTLFLDEIGDMPLSLQVKLLRVLQERKVRPLGSD
RDCAIDVRILSPPIGICPR
>Mature_258_residues
TARRHAHLLLVDDDTSLLKLLGMRLTSEGFTVTTAENGQEALRLLQRGKIDLVISDLRMDEMDGIALFKAIQQQPGLPVI
ILTAHGSIPDAVSATQEGVFSFLTKPVDRDALYCAIDGALALTRLAGDERWWDAIVTRSPLMLRLLEQAKMVAQSDVSIL
ISGQSGTGKEILAKVIHAASPRAHARGAFTGVVSQREGLFQTADGGTLFLDEIGDMPLSLQVKLLRVLQERKVRPLGSDR
DCAIDVRILSPPIGICPR

Specific function: Probable member of a two-component regulatory system yfhA/yfhK [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=273, Percent_Identity=70.6959706959707, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1790437, Length=272, Percent_Identity=40.8088235294118, Blast_Score=190, Evalue=9e-50,
Organism=Escherichia coli, GI1788550, Length=277, Percent_Identity=37.9061371841155, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1790299, Length=273, Percent_Identity=39.9267399267399, Blast_Score=181, Evalue=4e-47,
Organism=Escherichia coli, GI87082117, Length=139, Percent_Identity=45.3237410071942, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI87082152, Length=154, Percent_Identity=44.1558441558442, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1789087, Length=159, Percent_Identity=44.0251572327044, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1789233, Length=149, Percent_Identity=37.5838926174497, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786524, Length=236, Percent_Identity=29.6610169491525, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1789828, Length=143, Percent_Identity=35.6643356643357, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787583, Length=140, Percent_Identity=35, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI87081872, Length=142, Percent_Identity=33.0985915492958, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1788191, Length=109, Percent_Identity=34.8623853211009, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI145693157, Length=106, Percent_Identity=37.7358490566038, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1789809, Length=180, Percent_Identity=27.7777777777778, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 28139; Mature: 28008

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARRHAHLLLVDDDTSLLKLLGMRLTSEGFTVTTAENGQEALRLLQRGKIDLVISDLRM
CCCCCCEEEEEEECCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHCCCEEEEEECCCC
DEMDGIALFKAIQQQPGLPVIILTAHGSIPDAVSATQEGVFSFLTKPVDRDALYCAIDGA
CCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECHH
LALTRLAGDERWWDAIVTRSPLMLRLLEQAKMVAQSDVSILISGQSGTGKEILAKVIHAA
HHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHC
SPRAHARGAFTGVVSQREGLFQTADGGTLFLDEIGDMPLSLQVKLLRVLQERKVRPLGSD
CCCCHHCHHHHHHHHHCCCCEEECCCCEEEEEHHCCCCHHHHHHHHHHHHHCCCCCCCCC
RDCAIDVRILSPPIGICPR
CCCEEEEEEECCCCCCCCC
>Mature Secondary Structure 
TARRHAHLLLVDDDTSLLKLLGMRLTSEGFTVTTAENGQEALRLLQRGKIDLVISDLRM
CCCCCEEEEEEECCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHCCCEEEEEECCCC
DEMDGIALFKAIQQQPGLPVIILTAHGSIPDAVSATQEGVFSFLTKPVDRDALYCAIDGA
CCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECHH
LALTRLAGDERWWDAIVTRSPLMLRLLEQAKMVAQSDVSILISGQSGTGKEILAKVIHAA
HHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHC
SPRAHARGAFTGVVSQREGLFQTADGGTLFLDEIGDMPLSLQVKLLRVLQERKVRPLGSD
CCCCHHCHHHHHHHHHCCCCEEECCCCEEEEEHHCCCCHHHHHHHHHHHHHCCCCCCCCC
RDCAIDVRILSPPIGICPR
CCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]