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The map label for this gene is nuoI

Identifier: 85059572

GI number: 85059572

Start: 2739356

End: 2739898

Strand: Reverse

Name: nuoI

Synonym: SG1594

Alternate gene names: 85059572

Gene position: 2739898-2739356 (Counterclockwise)

Preceding gene: 85059573

Following gene: 85059571

Centisome position: 65.69

GC content: 57.27

Gene sequence:

>543_bases
ATGACGTTAAAAGAGTTAGCGATCGGCTGCGGGACCACGCTGCGCAGCATCTGGATGATTGGCATGCAGGCGTTCAACAA
GCGTGAAACCCGCATGTACCCGGACGTGCCTGTTAATCCGCCACCCCGTTTCCGCGGGCGGATCGTGCTGACGCGCGATC
CCGACGGCGATGAGCGCTGCGTGGCCTGTAACCTGTGCGCGGTCGCCTGCCCGGTCGACTGTATTTCGCTGCAAAAGGCG
GAGACCAAAGACGGGCGCTGGTACCCGGAATTCTTTCGTATCAATTTTTCCCGCTGTATCTTCTGCGGCCTGTGCGAAGA
AGCCTGTCCCACCACCGCCATCCAGCTCACGCCGGATTTCGAGATGGGCGAGTTCAAACGGCAGGATCTGGTGTATGAGA
AAGACGATTTACTGATTTCAGGGCCGGGTAAATACCCGGAATATAACTTCTACCGCATGGCCGGTATGGCGATTGCTGGC
AAGGACAAGGGCGAGGCGGAAAACGAAGCCAAACCTATCGACGTGAAAGGTTTGCTGCCCTAA

Upstream 100 bases:

>100_bases
TGGCGCTGGGCTGGAAAATCTGCCTGCCGCTGACGCTGCTCAATTTGTTGGCGACCGCAGCGGTCATCTTGTACAACGCG
CATTAAGGGGTGGATGAATC

Downstream 100 bases:

>100_bases
GGAGACTGGCATGGAACTGGCGTTTTATCTTTCCGGTCTGGTGGCGGTGCTGGCGACGTTGCGCGTCATCACCCATACCC
AGCCGGTACATGCGCTGCTG

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I

Number of amino acids: Translated: 180; Mature: 179

Protein sequence:

>180_residues
MTLKELAIGCGTTLRSIWMIGMQAFNKRETRMYPDVPVNPPPRFRGRIVLTRDPDGDERCVACNLCAVACPVDCISLQKA
ETKDGRWYPEFFRINFSRCIFCGLCEEACPTTAIQLTPDFEMGEFKRQDLVYEKDDLLISGPGKYPEYNFYRMAGMAIAG
KDKGEAENEAKPIDVKGLLP

Sequences:

>Translated_180_residues
MTLKELAIGCGTTLRSIWMIGMQAFNKRETRMYPDVPVNPPPRFRGRIVLTRDPDGDERCVACNLCAVACPVDCISLQKA
ETKDGRWYPEFFRINFSRCIFCGLCEEACPTTAIQLTPDFEMGEFKRQDLVYEKDDLLISGPGKYPEYNFYRMAGMAIAG
KDKGEAENEAKPIDVKGLLP
>Mature_179_residues
TLKELAIGCGTTLRSIWMIGMQAFNKRETRMYPDVPVNPPPRFRGRIVLTRDPDGDERCVACNLCAVACPVDCISLQKAE
TKDGRWYPEFFRINFSRCIFCGLCEEACPTTAIQLTPDFEMGEFKRQDLVYEKDDLLISGPGKYPEYNFYRMAGMAIAGK
DKGEAENEAKPIDVKGLLP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI4505371, Length=137, Percent_Identity=37.956204379562, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI1788617, Length=180, Percent_Identity=89.4444444444444, Blast_Score=336, Evalue=5e-94,
Organism=Escherichia coli, GI1789075, Length=105, Percent_Identity=33.3333333333333, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17555194, Length=145, Percent_Identity=36.551724137931, Blast_Score=97, Evalue=5e-21,
Organism=Drosophila melanogaster, GI17864306, Length=131, Percent_Identity=38.1679389312977, Blast_Score=101, Evalue=3e-22,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOI_SODGM (Q2NSK6)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_455274.1
- ProteinModelPortal:   Q2NSK6
- SMR:   Q2NSK6
- STRING:   Q2NSK6
- GeneID:   3867889
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG1594
- NMPDR:   fig|343509.6.peg.3402
- eggNOG:   COG1143
- HOGENOM:   HBG724359
- OMA:   RYWSVAG
- PhylomeDB:   Q2NSK6
- ProtClustDB:   PRK05888
- BioCyc:   SGLO343509:SG1594-MONOMER
- HAMAP:   MF_01351
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226
- Gene3D:   G3DSA:1.10.1060.10
- TIGRFAMs:   TIGR01971

Pfam domain/function: PF00037 Fer4

EC number: =1.6.99.5

Molecular weight: Translated: 20320; Mature: 20189

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
9.4 %Cys+Met (Translated Protein)
5.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
8.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKELAIGCGTTLRSIWMIGMQAFNKRETRMYPDVPVNPPPRFRGRIVLTRDPDGDERC
CCHHHHHHHCCHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCEEEEEECCCCCCEE
VACNLCAVACPVDCISLQKAETKDGRWYPEFFRINFSRCIFCGLCEEACPTTAIQLTPDF
EEEEEEEECCCHHHHHCCCCCCCCCCCCCHHEEECHHHEEEEEHHHHCCCCCEEEECCCC
EMGEFKRQDLVYEKDDLLISGPGKYPEYNFYRMAGMAIAGKDKGEAENEAKPIDVKGLLP
CCCCHHHHCCCEECCCEEEECCCCCCCCCEEEEECEEEECCCCCCCCCCCCCCCEECCCC
>Mature Secondary Structure 
TLKELAIGCGTTLRSIWMIGMQAFNKRETRMYPDVPVNPPPRFRGRIVLTRDPDGDERC
CHHHHHHHCCHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCEEEEEECCCCCCEE
VACNLCAVACPVDCISLQKAETKDGRWYPEFFRINFSRCIFCGLCEEACPTTAIQLTPDF
EEEEEEEECCCHHHHHCCCCCCCCCCCCCHHEEECHHHEEEEEHHHHCCCCCEEEECCCC
EMGEFKRQDLVYEKDDLLISGPGKYPEYNFYRMAGMAIAGKDKGEAENEAKPIDVKGLLP
CCCCHHHHCCCEECCCEEEECCCCCCCCCEEEEECEEEECCCCCCCCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA