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The map label for this gene is rob [H]

Identifier: 85059527

GI number: 85059527

Start: 2641718

End: 2642323

Strand: Reverse

Name: rob [H]

Synonym: SG1549

Alternate gene names: 85059527

Gene position: 2642323-2641718 (Counterclockwise)

Preceding gene: 85059528

Following gene: 85059524

Centisome position: 63.35

GC content: 55.45

Gene sequence:

>606_bases
ATGGATCAGGCCGGTATTATCCAAGATTTGTTGAGCTGGCTGGAAGGGCATCTCGATACGCCGTTGACGCTGAATAGGGT
GGCTGAGCGCTCGGGTTATTCCAAGTGGCACCTGCAGCGTAAATTTAAGCTGGCGACCGGGCAGGCGATTGGCGCTTATA
TCCGGGCACGGCGGCTCTCGCGGGCCGTGTTGGCGTTGCGGCTCACCAGCCGATCGGTAGCCGATATCGCACTGCAATAC
GGTTTCGATTCGCAACAAACCTTCACCCGCCCTTTTAAAAAACAATTTATACAAACGCCGGCGCTTTATCGCCGTTCGCG
TAAATGGCATTTCTTGGGCATGTACCCGCCGTATCGGTTCGATAGCCGGCCTTTGCCCCAGGCGGAATTCGTTACCTTGC
AGGTGATGACGCTTATCGGAACGTCGCAAAGCTATACCTGCACTCTGGAGCAACTGAGTGAATATCGCACCAATTTGCGA
TCGCATTTCTGGCGCCAGTTCCTGGCCGCGATGCCAACCAAACCGCCGGTGCTCTACGGTCTGTCGCCACACAGGCGATC
AAGGCACGAGAAGATCGTCAGGAAGTGTTCTATACCACAGCGGTAG

Upstream 100 bases:

>100_bases
TTATTAGCATAAGTGTTATAAAGCTTCCGGCGATATCAGTGCATCTCAGGCGGATGCGGTGGGATGACATCAAAACTTGC
AAGGAACTTGAGGGTATTTT

Downstream 100 bases:

>100_bases
AACCGCAATACCTGACCGACGGCATGCGTCAGGGGCAGCCCATTGCGCTTGAGGCAGGTGAATATATTCAATTTACTTAT
CGCGGGCCAAAGCCGGGACT

Product: putative right origin-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MDQAGIIQDLLSWLEGHLDTPLTLNRVAERSGYSKWHLQRKFKLATGQAIGAYIRARRLSRAVLALRLTSRSVADIALQY
GFDSQQTFTRPFKKQFIQTPALYRRSRKWHFLGMYPPYRFDSRPLPQAEFVTLQVMTLIGTSQSYTCTLEQLSEYRTNLR
SHFWRQFLAAMPTKPPVLYGLSPHRRSRHEKIVRKCSIPQR

Sequences:

>Translated_201_residues
MDQAGIIQDLLSWLEGHLDTPLTLNRVAERSGYSKWHLQRKFKLATGQAIGAYIRARRLSRAVLALRLTSRSVADIALQY
GFDSQQTFTRPFKKQFIQTPALYRRSRKWHFLGMYPPYRFDSRPLPQAEFVTLQVMTLIGTSQSYTCTLEQLSEYRTNLR
SHFWRQFLAAMPTKPPVLYGLSPHRRSRHEKIVRKCSIPQR
>Mature_201_residues
MDQAGIIQDLLSWLEGHLDTPLTLNRVAERSGYSKWHLQRKFKLATGQAIGAYIRARRLSRAVLALRLTSRSVADIALQY
GFDSQQTFTRPFKKQFIQTPALYRRSRKWHFLGMYPPYRFDSRPLPQAEFVTLQVMTLIGTSQSYTCTLEQLSEYRTNLR
SHFWRQFLAAMPTKPPVLYGLSPHRRSRHEKIVRKCSIPQR

Specific function: Binds to the right arm of the replication origin oriC of the chromosome. Rob binding may influence the formation of the nucleoprotein structure, required for oriC function in the initiation of replication [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790857, Length=191, Percent_Identity=62.82722513089, Blast_Score=251, Evalue=2e-68,
Organism=Escherichia coli, GI1790497, Length=104, Percent_Identity=51.9230769230769, Blast_Score=117, Evalue=5e-28,
Organism=Escherichia coli, GI87081928, Length=101, Percent_Identity=47.5247524752475, Blast_Score=105, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010499
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR011256 [H]

Pfam domain/function: PF06445 AraC_E_bind; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 23530; Mature: 23530

Theoretical pI: Translated: 11.34; Mature: 11.34

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQAGIIQDLLSWLEGHLDTPLTLNRVAERSGYSKWHLQRKFKLATGQAIGAYIRARRLS
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
RAVLALRLTSRSVADIALQYGFDSQQTFTRPFKKQFIQTPALYRRSRKWHFLGMYPPYRF
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCEEEECCCCCCC
DSRPLPQAEFVTLQVMTLIGTSQSYTCTLEQLSEYRTNLRSHFWRQFLAAMPTKPPVLYG
CCCCCCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEC
LSPHRRSRHEKIVRKCSIPQR
CCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDQAGIIQDLLSWLEGHLDTPLTLNRVAERSGYSKWHLQRKFKLATGQAIGAYIRARRLS
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
RAVLALRLTSRSVADIALQYGFDSQQTFTRPFKKQFIQTPALYRRSRKWHFLGMYPPYRF
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCEEEECCCCCCC
DSRPLPQAEFVTLQVMTLIGTSQSYTCTLEQLSEYRTNLRSHFWRQFLAAMPTKPPVLYG
CCCCCCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEC
LSPHRRSRHEKIVRKCSIPQR
CCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]