Click here to switch to the map view.

The map label for this gene is codB [H]

Identifier: 85059457

GI number: 85059457

Start: 2472225

End: 2473538

Strand: Reverse

Name: codB [H]

Synonym: SG1479

Alternate gene names: 85059457

Gene position: 2473538-2472225 (Counterclockwise)

Preceding gene: 85059458

Following gene: 85059456

Centisome position: 59.3

GC content: 52.36

Gene sequence:

>1314_bases
ATGGATATTTCTATGAATGATGATTTTGTTTATGGCAGGGTTCCCCGTGCGCAGCGTTCAACTCTTTTCACCATCACGTT
AATACGAATAGGCTCTATCACCGCGCTGTCGCAATTCATGCTGGGGGCAACGCTTGGGCATGCTATGTCATTTTGGCAAG
CGATGCTGGCGACCCTGTTGGGTAGCCTGATTTTGGAGTTTGTCGGTCTGGGACTGGGCCTTGCCGGCGTGCGCGAAGGG
CTGCCTACTTCGTTGCTTTCCCGCTGGTGCGGTTTTGGCCGTTTGGGGTCCGCCCTCATTGGGCTGGCGATAGGCATAAG
CTCGCTCGGCTGGTTTGGCGTGCAAAACGGCATTTTTGCCAAAAGTCTGGCTTATGCTTTTGAGGGCAAATTATCTTTTG
CCTGGTGCGCCGTCATCTCGGGTTTGGCCACTACGCTGCTTGTCGCTTTTGGCTATCGCGCCTTGGGCTGGACGGCGAGA
ATTGCTGTGCCGCTTTTTATCATGATCGTGGTTTGGGCTGCTTATCATGTATTGCCGGGGAATAGCACCGCCGAGTTGTT
CGGATACGCCCGCGATCGCGCGACAACGATTACGCTGAGCGCCGGGGCAACGATGGTGGCGGGCGGCTATATTGTTGCAG
CGTTAATTACGCCGGATATCAGCCGCTATTGCAAAGACGGTAAAAGCGTATTCTGGATGATGACGATCTCTATTATCGTT
GGCGAGTTCATTGTCAATGCGTTGTCGATTTTAATTGCGCATGTGCTCGATACAGAAGACGTGGTGAGTATCATGACGCA
AAGCGCCGGTTGGTTGGGCGCTGCCTCGGTGATGCTCGCGGTAGTGAAGGTCAATGACATTAATCTTTATAGCGTATCGC
TTGGGGTCAGTAATTTTATTCATGTCTGGTCCGGTTTGCGCATTAAATACAGCTACATCACCCTGATAGCTGGGTCGGTA
GGAACGCTATTCACCGTGACGGGAATTTTGCATAAATTTATCGATTTTCTGATGATGGTCGGCGTGGTTTTTCCTCCCAT
CGCCGGCATTATGTTAGTCGATTATTACCTTTTGCGCACCCACCGTGCCCTATTGGACGCTACTCGCCAGGAAGGGACGC
TTCCCGACAGAGCCGCAACGCCGTTCGTCAATGGGGTGGCTTTATTTTCCTGGGGCGTTGGCGCCGTCTCCGGCTATGTG
ATTACCGTGGGCATTCCTGCGCTGAATGCGATAGCCATCGCCGGCGCTCTATATTGGCTGTTGTCGCATCTTAAACATAA
TGATAGTAAACGCGTGTCTGAGGGTGAGAGGTAA

Upstream 100 bases:

>100_bases
GGCCTGCTTAAGATGGGAAGTTATCTGCATTTTCAATAAGTATTAACCCTAACGGCGCGGACGTCAGTCCAGAGCCGTAC
ATGGATTCACTTATTCCAAC

Downstream 100 bases:

>100_bases
CCATTGTTTACATTAGCTTTAGTACAAACAGATGCCTATTTGGGTGAAAAACGGAGAAATCTCGAACGGTTGCAGACATT
TTGCCATACCGCTAAAAAAG

Product: putative cytosine transport protein

Products: Proton [Cytoplasm]; cytosine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MDISMNDDFVYGRVPRAQRSTLFTITLIRIGSITALSQFMLGATLGHAMSFWQAMLATLLGSLILEFVGLGLGLAGVREG
LPTSLLSRWCGFGRLGSALIGLAIGISSLGWFGVQNGIFAKSLAYAFEGKLSFAWCAVISGLATTLLVAFGYRALGWTAR
IAVPLFIMIVVWAAYHVLPGNSTAELFGYARDRATTITLSAGATMVAGGYIVAALITPDISRYCKDGKSVFWMMTISIIV
GEFIVNALSILIAHVLDTEDVVSIMTQSAGWLGAASVMLAVVKVNDINLYSVSLGVSNFIHVWSGLRIKYSYITLIAGSV
GTLFTVTGILHKFIDFLMMVGVVFPPIAGIMLVDYYLLRTHRALLDATRQEGTLPDRAATPFVNGVALFSWGVGAVSGYV
ITVGIPALNAIAIAGALYWLLSHLKHNDSKRVSEGER

Sequences:

>Translated_437_residues
MDISMNDDFVYGRVPRAQRSTLFTITLIRIGSITALSQFMLGATLGHAMSFWQAMLATLLGSLILEFVGLGLGLAGVREG
LPTSLLSRWCGFGRLGSALIGLAIGISSLGWFGVQNGIFAKSLAYAFEGKLSFAWCAVISGLATTLLVAFGYRALGWTAR
IAVPLFIMIVVWAAYHVLPGNSTAELFGYARDRATTITLSAGATMVAGGYIVAALITPDISRYCKDGKSVFWMMTISIIV
GEFIVNALSILIAHVLDTEDVVSIMTQSAGWLGAASVMLAVVKVNDINLYSVSLGVSNFIHVWSGLRIKYSYITLIAGSV
GTLFTVTGILHKFIDFLMMVGVVFPPIAGIMLVDYYLLRTHRALLDATRQEGTLPDRAATPFVNGVALFSWGVGAVSGYV
ITVGIPALNAIAIAGALYWLLSHLKHNDSKRVSEGER
>Mature_437_residues
MDISMNDDFVYGRVPRAQRSTLFTITLIRIGSITALSQFMLGATLGHAMSFWQAMLATLLGSLILEFVGLGLGLAGVREG
LPTSLLSRWCGFGRLGSALIGLAIGISSLGWFGVQNGIFAKSLAYAFEGKLSFAWCAVISGLATTLLVAFGYRALGWTAR
IAVPLFIMIVVWAAYHVLPGNSTAELFGYARDRATTITLSAGATMVAGGYIVAALITPDISRYCKDGKSVFWMMTISIIV
GEFIVNALSILIAHVLDTEDVVSIMTQSAGWLGAASVMLAVVKVNDINLYSVSLGVSNFIHVWSGLRIKYSYITLIAGSV
GTLFTVTGILHKFIDFLMMVGVVFPPIAGIMLVDYYLLRTHRALLDATRQEGTLPDRAATPFVNGVALFSWGVGAVSGYV
ITVGIPALNAIAIAGALYWLLSHLKHNDSKRVSEGER

Specific function: Required for cytosine transport into the cell [H]

COG id: COG1457

COG function: function code F; Purine-cytosine permease and related proteins

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI1786530, Length=423, Percent_Identity=27.6595744680851, Blast_Score=108, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 46826; Mature: 46826

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDISMNDDFVYGRVPRAQRSTLFTITLIRIGSITALSQFMLGATLGHAMSFWQAMLATLL
CCCCCCCCEEECCCCCCCCCEEEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSLILEFVGLGLGLAGVREGLPTSLLSRWCGFGRLGSALIGLAIGISSLGWFGVQNGIFA
HHHHHHHHHHCCCHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
KSLAYAFEGKLSFAWCAVISGLATTLLVAFGYRALGWTARIAVPLFIMIVVWAAYHVLPG
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NSTAELFGYARDRATTITLSAGATMVAGGYIVAALITPDISRYCKDGKSVFWMMTISIIV
CCHHHHHHHHCCCCEEEEEECCCHHHCCHHHEEEHHCCCHHHHHCCCCCEEHHHHHHHHH
GEFIVNALSILIAHVLDTEDVVSIMTQSAGWLGAASVMLAVVKVNDINLYSVSLGVSNFI
HHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHEEECCCEEEEEHHHHHHHH
HVWSGLRIKYSYITLIAGSVGTLFTVTGILHKFIDFLMMVGVVFPPIAGIMLVDYYLLRT
HHHCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HRALLDATRQEGTLPDRAATPFVNGVALFSWGVGAVSGYVITVGIPALNAIAIAGALYWL
HHHHHHHHHCCCCCCCCCCCCHHCCHHHHHCCHHHHCCEEEEEECCHHHHHHHHHHHHHH
LSHLKHNDSKRVSEGER
HHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MDISMNDDFVYGRVPRAQRSTLFTITLIRIGSITALSQFMLGATLGHAMSFWQAMLATLL
CCCCCCCCEEECCCCCCCCCEEEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSLILEFVGLGLGLAGVREGLPTSLLSRWCGFGRLGSALIGLAIGISSLGWFGVQNGIFA
HHHHHHHHHHCCCHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
KSLAYAFEGKLSFAWCAVISGLATTLLVAFGYRALGWTARIAVPLFIMIVVWAAYHVLPG
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NSTAELFGYARDRATTITLSAGATMVAGGYIVAALITPDISRYCKDGKSVFWMMTISIIV
CCHHHHHHHHCCCCEEEEEECCCHHHCCHHHEEEHHCCCHHHHHCCCCCEEHHHHHHHHH
GEFIVNALSILIAHVLDTEDVVSIMTQSAGWLGAASVMLAVVKVNDINLYSVSLGVSNFI
HHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHEEECCCEEEEEHHHHHHHH
HVWSGLRIKYSYITLIAGSVGTLFTVTGILHKFIDFLMMVGVVFPPIAGIMLVDYYLLRT
HHHCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HRALLDATRQEGTLPDRAATPFVNGVALFSWGVGAVSGYVITVGIPALNAIAIAGALYWL
HHHHHHHHHCCCCCCCCCCCCHHCCHHHHHCCHHHHCCEEEEEECCHHHHHHHHHHHHHH
LSHLKHNDSKRVSEGER
HHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; cytosine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + cytosine [Periplasm] = Proton [Cytoplasm] + cytosine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]