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The map label for this gene is hns [H]

Identifier: 85059348

GI number: 85059348

Start: 2273539

End: 2273946

Strand: Reverse

Name: hns [H]

Synonym: SG1370

Alternate gene names: 85059348

Gene position: 2273946-2273539 (Counterclockwise)

Preceding gene: 85059350

Following gene: 85059343

Centisome position: 54.52

GC content: 48.28

Gene sequence:

>408_bases
ATGAGCGAAGCACTAAAGATTCTTAACAACATCCGTACTCTGCGCGCACAGGCCAGAGAATGCACTCTGGAAACTCTTGA
AGAAATGCTGGAGAAACTGGAGGTCGTTGTCAATGAACGCCGTGAGGAAGATAGTCAAGCCCAGGCTGAAATTGAAGAGC
ATACGCGTAAATTACAACAATACCGTGAAATGTTGATTGCCGATGGAATCGACCCGAACGAATTGCTGCAGTCGATGAGT
TCCTCCAAATCCGCTGGTAAAACCAAGCGTGCCGCTCGTCCGGCGAAATATCAGTACACCGACGAAAACGGCGACGTTAA
AACCTGGACCGGTCAAGGCCGTACCCCTGCGGTAATCAAAAAAGCGATTGATGAAGAAGGCAAAGCACTGGAAGATTTCC
TGCTATAA

Upstream 100 bases:

>100_bases
AAGAATATTTTTGATTAATCACAATCTGGGTATTGCAGAAAAGAAACTAGCGCTCTATTATTACTGGGACGCCCCCTTTA
TAATATTTGAGATTAAGTCA

Downstream 100 bases:

>100_bases
TCGCCTGCTTGATCCAGGTTACAAAAAACCCGCTGCCCGCAGCGGTTTTTTTTATGTGCCACCGGCGGCGGCGGCGACGG
CGACAAACGCCCTATCCGTT

Product: global DNA-binding transcriptional dual regulator H-NS

Products: NA

Alternate protein names: Histone-like protein HLP-II [H]

Number of amino acids: Translated: 135; Mature: 134

Protein sequence:

>135_residues
MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEDSQAQAEIEEHTRKLQQYREMLIADGIDPNELLQSMS
SSKSAGKTKRAARPAKYQYTDENGDVKTWTGQGRTPAVIKKAIDEEGKALEDFLL

Sequences:

>Translated_135_residues
MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEDSQAQAEIEEHTRKLQQYREMLIADGIDPNELLQSMS
SSKSAGKTKRAARPAKYQYTDENGDVKTWTGQGRTPAVIKKAIDEEGKALEDFLL
>Mature_134_residues
SEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEDSQAQAEIEEHTRKLQQYREMLIADGIDPNELLQSMSS
SKSAGKTKRAARPAKYQYTDENGDVKTWTGQGRTPAVIKKAIDEEGKALEDFLL

Specific function: H-NS binds tightly to ds-DNA, increases its thermal stability and inhibits transcription. It also binds to ss-DNA and RNA but with a much lower affinity. H-NS has possible histone-like function. May be a global transcriptional regulator through its abilit

COG id: COG2916

COG function: function code R; DNA-binding protein H-NS

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the histone-like protein H-NS family [H]

Homologues:

Organism=Escherichia coli, GI1787489, Length=135, Percent_Identity=82.962962962963, Blast_Score=208, Evalue=1e-55,
Organism=Escherichia coli, GI1789023, Length=135, Percent_Identity=54.8148148148148, Blast_Score=129, Evalue=5e-32,

Paralogues:

None

Copy number: 820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1780 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 1020 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 4632 Molecules/Cell In: Gr

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001801 [H]

Pfam domain/function: PF00816 Histone_HNS [H]

EC number: NA

Molecular weight: Translated: 15382; Mature: 15250

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEDSQAQAEIEEHTRKLQQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YREMLIADGIDPNELLQSMSSSKSAGKTKRAARPAKYQYTDENGDVKTWTGQGRTPAVIK
HHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCEECCCCCCEEECCCCCCCHHHHH
KAIDEEGKALEDFLL
HHHHHCCHHHHHHCC
>Mature Secondary Structure 
SEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEDSQAQAEIEEHTRKLQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YREMLIADGIDPNELLQSMSSSKSAGKTKRAARPAKYQYTDENGDVKTWTGQGRTPAVIK
HHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCEECCCCCCEEECCCCCCCHHHHH
KAIDEEGKALEDFLL
HHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2494066 [H]