Click here to switch to the map view.

The map label for this gene is purT

Identifier: 85059250

GI number: 85059250

Start: 2139581

End: 2140759

Strand: Reverse

Name: purT

Synonym: SG1272

Alternate gene names: 85059250

Gene position: 2140759-2139581 (Counterclockwise)

Preceding gene: 85059252

Following gene: 85059249

Centisome position: 51.32

GC content: 60.31

Gene sequence:

>1179_bases
ATGATCACCATCGGAACAGCACTTCGTCCGGCAGCAACGCGCGTGATGTTGCTCGGCGCCGGCGAACTGGGTAAAGAAGT
CGCTATTGAATGTCAGCGTTTGGGGATAGAGGTGATTGCCGTAGGCCACTATGCCGACGCCCCGGCGATGCACATCGCGC
ATCGCCATCACGTGATCAATATGTTGGATGGCGCAGCGCTTCGCGCCGTGATCGAACATGAGCGCCCGCACTTTATCGTG
CCGGAAATCGAGGCCATTGCCACCGACATGCTGGTCACCCTCGAACAAGAAGGGCACCACGTGGTACCCTGCGCTAACGC
GACACGGTTGACTATGAATCGGGAAGGTATTCGCCGGCTGGCGGCGGAAACGCTCTCTCTTCCCACCTCGCGCTACCGCT
TTGCCGCCGATCGGGCGGAACTTGATAAAGCGGTCGAGGCCATCGGTTATCCCTTCATTATCAAACCCGTCATGAGCTCT
TCCGGCAAAGGGCAAAGCCTGGTGCGGGACGCAGCACAGTTGGATGCCGCCTGGCAATATGCCCAACTCGGTGGTCGGGC
CGGCGGTGGCAAGGTTATCGTCGAAGGGCTGGTGGCGTTTGATTTTGAAATCACCCTCTTGACCATCAACGCCGTAGACG
GCATCCATTTCTGCGCGCCCATCGGTCATCGGCAAGAAGACGGCGACTACCGCGAGTCCTGGCAGCCGCAACACATGAGC
GCGCTGGCGCGCCAGCGTGCGCAGACCATTGCCGCTGACGTCGTCACTGCTTTGGGCGGAAAAGGTTTATTCGGCGTCGA
ACTGTTTATCTGTGGCGACGATGTTATCTTCAGCGAAGTTTCCCCCCGCCCTCATGACACCGGCATGGTGACGCTGATTT
CGCAGGACTTGTCGGAATTTGCCCTGCATGTGCGCGCTTTTCTCGGACTGCCGATCGGCACTATCCGTCAATATGGTCCG
GCGGCCTCGGCGGTGATTTTGCCGGAGCTGCAGAGCGATAACGTCAGCTTTAGCGGCCTGGAGAACGCGCTGGCAGCCGG
GCAACAGATTCGTCTGTTCGGTAAACCCGACCTTAGCGGCAAACGCCGTCTCGGCGTGGCGCTCGCCGTGGCGGCGACGG
TGGAAGAGGCGGTGACGGTGGCGAAAGATGCGGCTGCCGCGGTGCGGGTCACCGGTTAA

Upstream 100 bases:

>100_bases
ATTAACCCAAGCGTTGATATTGTTGCCGCCTGTTGGTCCTGTTCAGATCGCTGAATTTCGTTTATACTGCGCGCAAATTT
TATTACCGCGGATTGACGAG

Downstream 100 bases:

>100_bases
GCCGTTTTGCGTCCCCGGTTCTTTGGCCGGGGAAAGCATCAGTGGGCGCTGATTTACCTGTCGGCCCCGCACAGAAACCC
CAGCAAACCCATCACACCTG

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MITIGTALRPAATRVMLLGAGELGKEVAIECQRLGIEVIAVGHYADAPAMHIAHRHHVINMLDGAALRAVIEHERPHFIV
PEIEAIATDMLVTLEQEGHHVVPCANATRLTMNREGIRRLAAETLSLPTSRYRFAADRAELDKAVEAIGYPFIIKPVMSS
SGKGQSLVRDAAQLDAAWQYAQLGGRAGGGKVIVEGLVAFDFEITLLTINAVDGIHFCAPIGHRQEDGDYRESWQPQHMS
ALARQRAQTIAADVVTALGGKGLFGVELFICGDDVIFSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPIGTIRQYGP
AASAVILPELQSDNVSFSGLENALAAGQQIRLFGKPDLSGKRRLGVALAVAATVEEAVTVAKDAAAAVRVTG

Sequences:

>Translated_392_residues
MITIGTALRPAATRVMLLGAGELGKEVAIECQRLGIEVIAVGHYADAPAMHIAHRHHVINMLDGAALRAVIEHERPHFIV
PEIEAIATDMLVTLEQEGHHVVPCANATRLTMNREGIRRLAAETLSLPTSRYRFAADRAELDKAVEAIGYPFIIKPVMSS
SGKGQSLVRDAAQLDAAWQYAQLGGRAGGGKVIVEGLVAFDFEITLLTINAVDGIHFCAPIGHRQEDGDYRESWQPQHMS
ALARQRAQTIAADVVTALGGKGLFGVELFICGDDVIFSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPIGTIRQYGP
AASAVILPELQSDNVSFSGLENALAAGQQIRLFGKPDLSGKRRLGVALAVAATVEEAVTVAKDAAAAVRVTG
>Mature_392_residues
MITIGTALRPAATRVMLLGAGELGKEVAIECQRLGIEVIAVGHYADAPAMHIAHRHHVINMLDGAALRAVIEHERPHFIV
PEIEAIATDMLVTLEQEGHHVVPCANATRLTMNREGIRRLAAETLSLPTSRYRFAADRAELDKAVEAIGYPFIIKPVMSS
SGKGQSLVRDAAQLDAAWQYAQLGGRAGGGKVIVEGLVAFDFEITLLTINAVDGIHFCAPIGHRQEDGDYRESWQPQHMS
ALARQRAQTIAADVVTALGGKGLFGVELFICGDDVIFSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPIGTIRQYGP
AASAVILPELQSDNVSFSGLENALAAGQQIRLFGKPDLSGKRRLGVALAVAATVEEAVTVAKDAAAAVRVTG

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=392, Percent_Identity=75.5102040816327, Blast_Score=612, Evalue=1e-176,
Organism=Escherichia coli, GI1786733, Length=308, Percent_Identity=25.6493506493506, Blast_Score=72, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6324702, Length=379, Percent_Identity=22.9551451187335, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_SODGM (Q2NTH8)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_454952.1
- ProteinModelPortal:   Q2NTH8
- SMR:   Q2NTH8
- STRING:   Q2NTH8
- GeneID:   3866382
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG1272
- NMPDR:   fig|343509.6.peg.2626
- eggNOG:   COG0027
- HOGENOM:   HBG516369
- OMA:   PDYIVPE
- PhylomeDB:   Q2NTH8
- ProtClustDB:   PRK09288
- BioCyc:   SGLO343509:SG1272-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: 2.1.2.- [C]

Molecular weight: Translated: 41848; Mature: 41848

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 82-82 BINDING 114-114 BINDING 155-155 BINDING 203-203 BINDING 267-267 BINDING 279-279 BINDING 286-286 BINDING 355-355

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITIGTALRPAATRVMLLGAGELGKEVAIECQRLGIEVIAVGHYADAPAMHIAHRHHVIN
CEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHH
MLDGAALRAVIEHERPHFIVPEIEAIATDMLVTLEQEGHHVVPCANATRLTMNREGIRRL
HHHHHHHHHHHHCCCCCEECCCHHHHHHHHEEEEECCCCEEEECCCCEEEEECHHHHHHH
AAETLSLPTSRYRFAADRAELDKAVEAIGYPFIIKPVMSSSGKGQSLVRDAAQLDAAWQY
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHCCCCCCHHHHHHHHHHHHHHHH
AQLGGRAGGGKVIVEGLVAFDFEITLLTINAVDGIHFCAPIGHRQEDGDYRESWQPQHMS
HHHCCCCCCCCEEEEEEHEEEEEEEEEEEECCCCEEEECCCCCCCCCCCHHHCCCHHHHH
ALARQRAQTIAADVVTALGGKGLFGVELFICGDDVIFSEVSPRPHDTGMVTLISQDLSEF
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEECCCCCCCCCCCEEEEHHHHHHHH
ALHVRAFLGLPIGTIRQYGPAASAVILPELQSDNVSFSGLENALAAGQQIRLFGKPDLSG
HHHHHHHHCCCHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCCC
KRRLGVALAVAATVEEAVTVAKDAAAAVRVTG
CHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
>Mature Secondary Structure
MITIGTALRPAATRVMLLGAGELGKEVAIECQRLGIEVIAVGHYADAPAMHIAHRHHVIN
CEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHH
MLDGAALRAVIEHERPHFIVPEIEAIATDMLVTLEQEGHHVVPCANATRLTMNREGIRRL
HHHHHHHHHHHHCCCCCEECCCHHHHHHHHEEEEECCCCEEEECCCCEEEEECHHHHHHH
AAETLSLPTSRYRFAADRAELDKAVEAIGYPFIIKPVMSSSGKGQSLVRDAAQLDAAWQY
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHCCCCCCHHHHHHHHHHHHHHHH
AQLGGRAGGGKVIVEGLVAFDFEITLLTINAVDGIHFCAPIGHRQEDGDYRESWQPQHMS
HHHCCCCCCCCEEEEEEHEEEEEEEEEEEECCCCEEEECCCCCCCCCCCHHHCCCHHHHH
ALARQRAQTIAADVVTALGGKGLFGVELFICGDDVIFSEVSPRPHDTGMVTLISQDLSEF
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEECCCCCCCCCCCEEEEHHHHHHHH
ALHVRAFLGLPIGTIRQYGPAASAVILPELQSDNVSFSGLENALAAGQQIRLFGKPDLSG
HHHHHHHHCCCHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCCC
KRRLGVALAVAATVEEAVTVAKDAAAAVRVTG
CHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA