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The map label for this gene is nagB

Identifier: 85058836

GI number: 85058836

Start: 1410022

End: 1410849

Strand: Reverse

Name: nagB

Synonym: SG0858

Alternate gene names: 85058836

Gene position: 1410849-1410022 (Counterclockwise)

Preceding gene: 85058839

Following gene: 85058806

Centisome position: 33.82

GC content: 50.72

Gene sequence:

>828_bases
ATGAGACTGATTCCATTAAACACCGCCGCTCAGGTTGGTCAGTGGGCCGCCCGTCATATCGTCAACCGTATCAACGCCTT
CACCCCCAGCGCCGACCGCCCGTTCATTCTCGGTCTGCCCACCGGCAGCACGCCGCTGGAAGCCTATAAAAGCCTGATTG
AATTGCATAAAGCGGGCCAGGTCAGCTTTAAGCATGTGGTGACGTTCAACATGGATGAATATGTCGGCTTACCCGCCGAA
CATCCCGAAAGCTACCATTCATTCATGTTTAATAACTTTTTTAATTATATCGACATTCCACGGGAAAACATCAACCTCCT
TAACGGCAATGCCCCGGATATTGATGCTGAATGTCGCCGTTATGAGGAGAAGATCAAATCTTACGGCAAAACTCACCTGT
TCATGGGCGGTGTCGGCAACGACGGCCACATCGCTTTTAATGAACCGGGTTCATCTTTGGCCTCGCGCACCCGCATCAAA
ACCCTGACCCAGGATACCCGTCGGGCCAATTCACGCTTCTTTAACAATGATATCGAGCAGGTACCCCGCTACGCTCTGAC
GGTGGGCGTCGGTACGCTGCTTGACGCAGAAGAGGTGATGATTCTGGTGATAGGGCACAATAAGGCCCACGCACTGCAGG
CGGCTGTAGAAGGTAATGTCAATCATATGTGGACCATCACTTGCCTACAGTTGCATGCTAAAGCCGTCATGGTGTGCGAT
GATGCGCCGCGCGATAAATGCGCCCATTTCCGTGAGATAAATGGTATCGCTGCGGCGTTTCAATATGTGCAAAAGAATTA
CAATATTAGTGTGTTCTACTTCCTATAA

Upstream 100 bases:

>100_bases
TGCCCCTAAGGGGGGGACAACACGTCGGATTCCAGCGTGGACGCCCAGCGGTTATACTCCCCGGTCAAACACTGCACTGC
AACATATTACGAGGACAAAC

Downstream 100 bases:

>100_bases
ACATCCACCGTGAGATTAACTGGCCCTTTCAGCCGGAGGCGTAGGCCAATTCATCTCTGGCGCAGTGCTGGTATCAACAG
CTTGTAATTTTTTAATATAG

Product: glucosamine-6-phosphate deaminase

Products: NA

Alternate protein names: GlcN6P deaminase; GNPDA; Glucosamine-6-phosphate isomerase [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MRLIPLNTAAQVGQWAARHIVNRINAFTPSADRPFILGLPTGSTPLEAYKSLIELHKAGQVSFKHVVTFNMDEYVGLPAE
HPESYHSFMFNNFFNYIDIPRENINLLNGNAPDIDAECRRYEEKIKSYGKTHLFMGGVGNDGHIAFNEPGSSLASRTRIK
TLTQDTRRANSRFFNNDIEQVPRYALTVGVGTLLDAEEVMILVIGHNKAHALQAAVEGNVNHMWTITCLQLHAKAVMVCD
DAPRDKCAHFREINGIAAAFQYVQKNYNISVFYFL

Sequences:

>Translated_275_residues
MRLIPLNTAAQVGQWAARHIVNRINAFTPSADRPFILGLPTGSTPLEAYKSLIELHKAGQVSFKHVVTFNMDEYVGLPAE
HPESYHSFMFNNFFNYIDIPRENINLLNGNAPDIDAECRRYEEKIKSYGKTHLFMGGVGNDGHIAFNEPGSSLASRTRIK
TLTQDTRRANSRFFNNDIEQVPRYALTVGVGTLLDAEEVMILVIGHNKAHALQAAVEGNVNHMWTITCLQLHAKAVMVCD
DAPRDKCAHFREINGIAAAFQYVQKNYNISVFYFL
>Mature_275_residues
MRLIPLNTAAQVGQWAARHIVNRINAFTPSADRPFILGLPTGSTPLEAYKSLIELHKAGQVSFKHVVTFNMDEYVGLPAE
HPESYHSFMFNNFFNYIDIPRENINLLNGNAPDIDAECRRYEEKIKSYGKTHLFMGGVGNDGHIAFNEPGSSLASRTRIK
TLTQDTRRANSRFFNNDIEQVPRYALTVGVGTLLDAEEVMILVIGHNKAHALQAAVEGNVNHMWTITCLQLHAKAVMVCD
DAPRDKCAHFREINGIAAAFQYVQKNYNISVFYFL

Specific function: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion [H]

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily [H]

Homologues:

Organism=Homo sapiens, GI13027378, Length=241, Percent_Identity=60.9958506224066, Blast_Score=313, Evalue=8e-86,
Organism=Homo sapiens, GI19923881, Length=241, Percent_Identity=59.7510373443983, Blast_Score=308, Evalue=5e-84,
Organism=Escherichia coli, GI1786893, Length=241, Percent_Identity=83.402489626556, Blast_Score=436, Evalue=1e-124,
Organism=Escherichia coli, GI1789530, Length=233, Percent_Identity=28.3261802575107, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI48994958, Length=240, Percent_Identity=26.25, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17554876, Length=241, Percent_Identity=56.8464730290456, Blast_Score=283, Evalue=9e-77,
Organism=Drosophila melanogaster, GI24581960, Length=241, Percent_Identity=61.4107883817427, Blast_Score=330, Evalue=4e-91,
Organism=Drosophila melanogaster, GI19920764, Length=241, Percent_Identity=61.4107883817427, Blast_Score=330, Evalue=4e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006148
- InterPro:   IPR004547
- InterPro:   IPR018321 [H]

Pfam domain/function: PF01182 Glucosamine_iso [H]

EC number: =3.5.99.6 [H]

Molecular weight: Translated: 30914; Mature: 30914

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLIPLNTAAQVGQWAARHIVNRINAFTPSADRPFILGLPTGSTPLEAYKSLIELHKAGQ
CEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCC
VSFKHVVTFNMDEYVGLPAEHPESYHSFMFNNFFNYIDIPRENINLLNGNAPDIDAECRR
CEEEEEEEECCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHH
YEEKIKSYGKTHLFMGGVGNDGHIAFNEPGSSLASRTRIKTLTQDTRRANSRFFNNDIEQ
HHHHHHHCCCEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VPRYALTVGVGTLLDAEEVMILVIGHNKAHALQAAVEGNVNHMWTITCLQLHAKAVMVCD
HCHHEEEECHHHHCCCCCEEEEEEECCHHHHHHHHHCCCCCEEEEEEEEEHHCCEEEEEC
DAPRDKCAHFREINGIAAAFQYVQKNYNISVFYFL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
>Mature Secondary Structure
MRLIPLNTAAQVGQWAARHIVNRINAFTPSADRPFILGLPTGSTPLEAYKSLIELHKAGQ
CEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCC
VSFKHVVTFNMDEYVGLPAEHPESYHSFMFNNFFNYIDIPRENINLLNGNAPDIDAECRR
CEEEEEEEECCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHH
YEEKIKSYGKTHLFMGGVGNDGHIAFNEPGSSLASRTRIKTLTQDTRRANSRFFNNDIEQ
HHHHHHHCCCEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VPRYALTVGVGTLLDAEEVMILVIGHNKAHALQAAVEGNVNHMWTITCLQLHAKAVMVCD
HCHHEEEECHHHHCCCCCEEEEEEECCHHHHHHHHHCCCCCEEEEEEEEEHHCCEEEEEC
DAPRDKCAHFREINGIAAAFQYVQKNYNISVFYFL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA